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All Journal Resona : Jurnal Ilmiah Pengabdian Masyarakat Chemistry Indonesian Journal of Chemical Research Molluca Journal of Chemistry Education (MJoCE) Agrokompleks Biofaal Journal Rumphius Pattimura Biological Journal Jurnal Rosenberg Teknologi Penangkapan Ikan Balobe: Jurnal Pengabdian Masyarakat Innovation for Community Service Journal
Hendro Hitijahubessy
Fisheries Biotechnology Study Program, Department Of Fisheries Product Technology, Tual State Fisheries Polytechnic, Jalan Raya. Langgur, Maluku, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Journal : Biofaal Journal

 1 
PENGARUH EKSTRAK ETANOL DAUN MANGKOKAN (Nothopanax scutellarium) YANG DIMANFAATKAN SEBAGAI ANTIBAKTERI DARI BAKTERI Vibrio sp Hitijahubessy, Hendro; Hanoatubun, Marthinus Imanuel Halaay; Fangohoi, Sesilia; Tumiwa, Bruri Berel; Metungun, Jakomina; Madubun, Usman
Biofaal Journal Vol 5 No 2 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i2pp091-099

Abstract

One of the pathogenic bacteria found in aquatic environments is Vibrio sp., which causes vibriosis. The use of mangkokan leaf extract as an antibacterial agent against Vibrio has not been studied before. The aim of this research was to evaluate the antibacterial activity of mangkokan leaf extract in inhibiting the growth of Vibrio sp. The antibacterial test in this study used the disc diffusion method. In addition, the study also conducted tests for yield and phytochemical analysis. The results showed that mangkokan leaf extract at concentrations of 100% and 75% exhibited moderate antibacterial activity, with average inhibition zone diameters of 8.5 mm and 6.35 mm, respectively. The yield of the ethanol extract from mangkokan leaves was 10.2%. The mangkokan leaf extract contains secondary metabolites such as flavonoids, steroids, alkaloids, tannins, and saponins.
UTILIZATION OF ACTIVATED CHARCOAL FROM NUTMEG SHELL WASTE AS AN ADSORBENT TO IMPROVE THE QUALITY OF VIRGIN COCONUT OIL (VCO) FERMENTATION METHOD Hitijahubessy, Hendro; Huwae, Laury Marcia Ch
Biofaal Journal Vol 2 No 2 (2021): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.599 KB) | DOI: 10.30598/biofaal.v2i2pp81-86

Abstract

Virgin coconut oil (VCO) is a form of processed coconut that is widely produced by people in Indonesia. VCO is often called pure coconut oil. VCO with good quality is by fermentation method using yeast. The quality of VCO can be measured by 3 types of analysis: water content, free fatty acids and peroxide number. The results of this analysis will be compared with the standards of the Asian and Pacific Coconut Community (APCC). VCO from fermentation method will be combined with the absorption method using nutmeg shell activated charcoal to see if there is a much better quality improvement. The results showed that there was a significant increase in quality with a decrease in the average water content from 0,1665% to 0,1005%. In addition, there was a significant decrease in the average free fatty acid content from 0,4681% to 0,3790% and significant decrease in average peroxide number from 0,1991 meq/kg to 0,0997 meq/kg. The results of the research on three VCO quality standards showed a value that was in accordance with the APCC standard.
ANTIBACTERIAL ACTIVITY EFFICACY OF EXTRACT SEAWEED Kappaphycus alvarezii AND LEAVES OF MANGROVE (Rhizophora apiculata) AGAINST Aeromonas hydrophilla CAUSES OF ICE-ICE DISEASE Hitijahubessy, Hendro; Bandjar, Muniati Nur Aisyah; Huwae, Laury Marcia Ch.; Hanoatubun, M. I. H.
Biofaal Journal Vol 4 No 1 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i1pp40-48

Abstract

Southeast Maluku area, seaweed is the livelihood of the community. However, in its cultivation there are some common obstacles that are often experienced. One of them is ice-ice disease caused by the presence of bacteria such as Aeromonas hydrophilla. Therefore, prevention that can be used as a solution is antibacterial. One of the natural ingredients that can be used as an antibacterial is seaweed, because it contains compounds that can inhibit bacterial growth. In general, the most common bioactive compounds found are flavonoids, phenols, and tannins. In addition to seaweed, mangrove plants also have compounds that can be antibacterial. The extract method used in this study is a simple extraction method that uses only one type of solvent, namely distilled water. And a comparison of concentrations between 100%, 80%, 60% and 40% was carried out. Then the inhibition zone test was carried out using the paper disc diffusion method, where the disc paper was dipped into each extract with different concentrations and placed in a petri dish that had been overgrown with bacteria. Measurement of the inhibition zone formed was carried out after the plates were incubated for 24 hours at 37oC. The measurement results show that both aquades extracts have medium to strong inhibitory power. The conclusion of this research is that aquades extract of seaweed and mangrove leaves has inhibitory power against Aeromonas hydrophilla bacteria.
UJI AKTIVITAS ANTIBAKTERI Vibrio sp. DENGAN EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L.) Sianturi, Anggreni; Hitijahubessy, Hendro; Rumangun, Anastasia Putri Marshanda; Samid, Aprianti
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp072-080

Abstract

Study of the antibacterial ability of soursop leaf extract (Annona muricata L.) in inhibiting the growth of Vibrio sp. The maceration method with 70% ethanol solvent was used in this research to determine the yield, then the extract was diluted in stages from a concentration of 100% to 3.125%. The antibacterial test was done using the disc diffusion method in TCBS media and a phytochemical test was carried out on soursop leaves to analyze the secondary metabolite content. The extract yield obtained in this study was 3.77%. The test results showed that the largest zone of inhibition was found at 100% extract concentration of 11.45mm. The smallest inhibition zone was found in the 6.25% extract with an inhibition zone of 1.44mm, the diameter of the positive control (tetracycline) inhibition zone was 22.75mm, the diameter of 10% DMSO as a negative control was 0 mm. Phytochemical tests of soursop leaves show that they contain alkaloids, flavonoids, steroids, saponins and tannins.
EFEKTIFITAS ANTIBAKTERI Vibrio sp. DARI EKSTRAK DAUN MANGROVE Rhizopora apiculata Hitijahubessy, Hendro; Irmawati, Yuni
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp081-089

Abstract

Research was carried out by analyzing the antibacterial ability of the ethanol extractof mangrove Rhizopora apiculata leaves against Vibrio sp. Maceration using 70%ethanol resulted in an extract yield of 3.09%. The highest average inhibition zoneresults were found in mangrove leaf extract at a concentration of 100% with aninhibition zone of 24.68 mm. The smallest inhibition zone was found in mangrove leafextract with a concentration of 6.25% with an inhibition zone of 3.85 mm, a positivecontrol inhibition zone (30 mg tetracycline) of 26.35 mm and a negative controlinhibition zone (DMSO 10%) of 0 mm. This can be seen from the results of theaverage inhibition zone which shows strong antibacterial activity of mangrove leafextract at a concentration of 100% and moderate antibacterial activity of mangroveleaf extract at a concentration of 75% to 50% and no antibacterial activity atconcentrations of mangrove leaf extract below 25 %, the positive control inhibitionzone (30 mg tetracycline) was 26.35 mm and the negative control inhibition zone(DMSO 10%) was 0 mm. Phytochemical analysis was also carried out on mangroveleaves with the results showing the presence of several visible secondary metabolites.The secondary metabolites found in Rhizopora apiculata mangrove leaves consist ofalkaloids, flavanoids, steroids, saponins and tannins.
ANALISIS KEMAMPUAN DARI EKSTRAK ETANOL RUMPUT LAUT Ulva lactuca SEBAGAI ANTIBAKTERI Vibrio sp. DAN KAJIAN FITOKIMIANYA Metungun, Jakomina; Beruatjaan, Mariana Yerminal; Hitijahubessy, Hendro; Tamher, Endang
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp100-107

Abstract

An analysis of the antibacterial ability of Vibrio sp. has been carried out from the ethanol extract of Ulva lactuca seaweed and its phytochemical study. Analysis of the antibacterial ability of Vibrio sp. using the disk diffusion method was the main thing carried out in this research, then the extract yield was calculated from the maceration process of Ulva lactuca seaweed, as well as phytochemical analysis to support the antibacterial ability of Ulva lactuca seaweed against the bacteria Vibrio sp. The results of the extraction of Ulva lactuca seaweed with ethanol solvent obtained a yield of 3.67%. The results showed a moderate antibacterial activity of Vibrio sp. from Ulva lactuca seaweed extract at a concentration of 100% was 17.3 mm. Weak antibacterial activity was at a concentration of 75% of 12.525 mm and extracts below a concentration of 75% did not have inhibitory power against Vibrio sp bacteria. Phytochemical studies of Ulva lactuca seaweed found secondary metabolite compounds in the form of alkaloids, flavonoids, steroids, tannins and saponins. Based on the results of this research, it is hoped that the antibacterial ability of Vibrio sp. can be further investigated from ethanol extract of Ulva lactuca seaweed in vivo.
ANALISIS KUALITAS HAND SANITIZER DARI KOMBINASI VIRGIN COCONUT OIL (VCO) SEBAGAI PELEMBUT DAN ANTIBAKTERI DENGAN CAMPURAN ETANOL Hitijahubessy, Hendro; Parlindungan, John Yoro
Biofaal Journal Vol 2 No 2 (2021): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.404 KB) | DOI: 10.30598/biofaal.v2i2pp87-93

Abstract

Era pandemi Covid-19 dalam kehidupan masyarakat sangat dibutuhkan pelindung diri yang dapat menangkal virus korona, salah satu pelindung diri yang digunakan saat ini adalah hand sanitizer. Penelitian ini akan dibuat hand sanitizer dengan kombinasi Virgin Coconut Oil (VCO) dengan alkohol. Fungsi kedua bahan ini sebagai antibakteri memungkinkan paduan kedua bahan ini sangat efektif untuk dikombinasikan menjadi produk hand sanitizer. Metode pengujian yang dilakukan dalam penelitian ini adalah uji organoleptic dan uji zona bening untuk mengetahu kualitas dari hand sanitizer. Hasil penelitian yang diperoleh melalui uji organoleptik dan uji zona bening dapat disimpulkan bahwa hand sanitizer yang terbaik berada pada konsentrasi VCO 25% karena sifat lembut di area telapak tangan dan kemampuan zona hambat yang sangat kuat terhadap bakteri yang berasal dari tangan manusia sebesar 20,05 mm. Pemanfaatan kelapa sebagai tanaman pesisir untuk dijadikan VCO dan bahan dasar pembuat hand sanitizer maka diharapkan dapat digunakan dengan mudah oleh masyarakat.
UJI AKTIVITAS ANTIBAKTERI Vibrio sp. DARI DAUN SERNAI (Wedelia biflora) Hitijahubessy, Hendro; Samid, Aprianti; Jalmaf, Welda Kristiyanti; Hasanela, Nurani; Huwae, Laury Marcia Ch
Biofaal Journal Vol 3 No 1 (2022): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v3i1pp43-50

Abstract

Penyakit Vibriosis pada ikan kakap, udang vaname dan rumput laut sering disebabkan disebabkan oleh bakteri Vibrio sp. Penyakit tersebut dapat dideteksi dengan mengisolasi bakteri dan menanamnya pada media agar selektif Vibrio sp. yaitu Thiosulfate Citrate Bile Sucrose (TCBS) agar. Daun sernai (Wedelia biflora) adalah tumbuhan yang banyak diteliti dan berguna sebagi antimikroba atau antibakteri. Berdasarkan kegunaan sebagai anti bakteri diharapkan dalam penelitian ini bahwa daun sernai dapat menjadi antibakteri Vibrio sp. Efektifitas dan efisiensi dari daun sernai sebagai antibakteri Vibrio sp. dalam penelitian ini dapat diperoleh dengan menentukan rendemen ekstrak, analisis fitokimia dan uji aktifitas ekstrak sebagai antibakteri Vibrio sp dengan metode disc diffusion Kirby-Bauer. Rendemen ekstrak daun sernai yang dihasilkan sebesar 11,7 %, dengan kandungan metabolit sekunder hasil uji fitokimia serbuk daun sernai menunjukan positif adanya senyawa alkaloid, terpenoid, flavonoid, dan saponin. Ekstrak daun sernai dengan konsentrasi 100 % mampu menghambat bakteri dengan respon hambat pertumbuhan bakteri Vibrio sp. yang kuat atau sensitif dan mempunyai respon hambat yang sama dengan tetrasiklin 30 mg.
ANALISIS KEMAMPUAN ANTIBAKTERI DARI EKSTRAK DAUN BELIMBING WULUH (Averrhoa bilimbi L.) TERHADAP Vibrio sp. Hitijahubessy, Hendro; Dumatubun, Alicia Royani; Hanoatubun, Marthinus Imanuel Halaay; Sianturi, Anggreni; Tumiwa, Bruri Berel
Biofaal Journal Vol 5 No 1 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i1pp053-064

Abstract

Pengobatan vibriosis dalam industri akuakultur memilih memberi makan ikan yang terinfeksi dengan obat antibiotik. Pemberian antibiotik sintesis bisa menyebabkan timbulnya resistensi antibiotik, dibutuhkan antibiotik baru dari bahan alam untuk mengurangi dampak bahaya bagi ikan, rumput laut, udang dan lingkungan sekitarnya. Salah satu tumbuhan yang dapat berfungsi sebagai antibakteri adalah tanaman belimbing wuluh. Penelitian ini bertujuan untuk memperoleh rendemen, mengukur kemampuan antibakteri Vibrio sp. dengan metode difusi cakram dan mengkaji kandungan metabolit sekunder dalam daun belimbing wuluh yang dilakukan maserasi dengan menggunakan pelarut etanol 70%. Tujuh perlakuan konsentrasi ekstrak yaitu ekstrak 100%, 75%, 50%, 25%, 12,5%, 6,25%, 3,125%. Hasil rendemen ekstrak etanol daun belimbing wuluh belimbing wuluh (Averrhoa bilimbi L.) sebesar 5%. Data hasil penelitian menunjukan adanya aktifitas antibakteri yang sedang dari ekstrak daun belimbing wuluh konsentrasi 100%, dengan zona hambat sebesar 16,95 mm. Aktifitas antibakteri yang lemah berada pada ekstrak konsentrasi 75% dengan zona hambat sebesar 11,75 mm. Konsentrasi ekstrak daun belimbing wuluh di bawah konsentrasi 75% tidak menghambat bakteri. Kajian fitokimia yang dilakukan dalam penelitian ini untuk mendukung sifat antibakteri Vibrio sp. Dari esktrak daun belimbing wuluh dan metabolit sekunder yang terkandung dalam daun belimbing wuluh adalah alkaloid, steroid, flavanoid, tanin dan saponin.
Co-Authors Alfianti Yuliastiwi Susiyanto Amahorseja, Anggeline Lioni Aprianti Samid Bandjar, Adriani Bandjar, Muniati Nur Aisyah Beruatjaan, Mariana Yerminal Dumatubun, Alicia Royani Fangohoi, Sesilia Hanoatubun, M. I. H. Hanoatubun, Marthinus Imanuel Halaay Hasanela, Nurani I. H. Hanoatubun, Marthinus Irmawati, Yuni Isti’anah, Ismi Jalmaf, Welda Kristiyanti Jamaludin Kabalmay, Jamaludin John Yoro Parlindungan Kabi Teniwut, Roberto Mario Latar, Dullah Irwan LAURY CHARA HUWAE Madubun, Usman Melissa Justine Renjaan Metungun, Jakomina Nara, Selfia Martha Nussy Lokra Latar Oscar Cesar R. Huwae, Jilian Rosulva, Indah Rumangun, Anastasia Putri Marshanda S. Kanuna, Resky Samid, Aprianti Samsul Bugis Sesilia Fangohoi Sianturi, Anggreni Tamher, Endang Tumiwa, Bruri Berel Warbal, Triyune Christovena Yanyaan, Virenze K.