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pH dan Total Mikroba pada Starter Mikroorganisme Lokal (MOL) Berbasis Limbah untuk Fermentasi Pakan Restuti Fitria; Novita Hindratiningrum; Muhammad Rayhan
Jurnal Sains Peternakan Vol 11 No 1 (2023): Jurnal Sains Peternakan
Publisher : Fakultas Peternakan, Universitas PGRI Kanjuruhan Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jsp.v11i1.7638

Abstract

Tujuan khusus dari penelitian adalah memanfaatkan limbah di sekitar wilayah Banyumas seperti sisa nasi limbah rumah tangga, ampas tahu dan onggok limbah industri olahan pangan serta sampah sayur limbah pasar untuk dijadikan sebagai starter fermentasi fungsional. Penelitian ini dilakukan dengan metode eksperimental dan terdapat 4 perlakuan. Data yang telah diperoleh kemudian dianalisis secara deskriptif kuantitatif. Adapun perlakuan dalam penelitian antara lain NF1: MOL asal nasi basi; NF2: MOL asal ampas tahu; NF3: MOL asal onggok; dan NF4: MOL asal sampah sayur. Variabel penelitian yang diamati adalah pH starter dan total mikroba. Hasil pengukuran pH starter MOL NF1, NF2, NF3 dan NF4 menunjukan pH yang asam (pH dibawah 7) yaitu berkisar 4,8 – 5,3dan hasil pengukuran total mikroba yang diperoleh baik pada starter NF1, NF2, NF3 dan NF4 menunjukan total mikroba yaitu 1.80x107CFU/ml - 3.08x107CFU/ml. Kesimpulan dari hasil yang diperoleh menunjukan bahwa starter yang berbasis limbah tersebut memiliki potensi untuk dijadikan sebagai starter fermentasi pakan untuk meningkatkan kualitas pakan bermutu rendah
PARAMETER RUMEN TERHADAP TONGKOL JAGUNG AMOFER MENGGUNAKAN STARTER KOMERSIAL DAN UREA PADA LEVEL YANG BERBEDA SECARA IN VITRO Restuti Fitria; Novita Hindratiningrum
Scientific Timeline Vol. 3 No. 01 (2023)
Publisher : UNU Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This research aimed to evaluate the levels of addition of M21 Decomposer (MD) and urea (U) to fermented products (concentrations of VFA and NH3) in the rumen. This research used an experimental method with a completely randomized design (CRD). The treatment in this research was the addition of MD and U at different levels (R0 = corn cob without amofer / control; R1 = corn cob amofer with 0.04% MD + 3% U; R2 = corn cob amofer with 0.06% MD + 3% U; R3 = corn cob amofer with 0.04% MD + 5% U; and R4 = corn cob amofer with 0.06% MD + 5% U). The results showed that treatment had no significant effect (P> 0.05) on the concentration of VFA and NH3. However, the results showed that the NH3 concentration tended to increase along with the increasing addition of urea. The conclusion is that the addition of MD and U at the level used has not been able to increase the concentration of VFA and NH3.
Upaya Peningkatan Keterampilan Pembuatan Starter Mikroorganisme Lokal (MOL) untuk Amofer Limbah Pertanian pada Kelompok Tani Ternak Kambing Terus Jaya, Desa Pegalongan, Banyumas Restuti Fitria; Novita Hindratiningrum; Muhammad Rayhan; Subhanul Arafat Chayatul Luthfi; Herdian Farisi
Jurnal Abdi Masyarakat Indonesia Vol 4 No 2 (2024): JAMSI - Maret 2024
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jamsi.1076

Abstract

Salah satu kelompok tani ternak kambing yang terdapat di Desa Pegalongan adalah KTT Terus Jaya. KTT Terus Jaya berlokasi di Dusun Cunil, Desa Pegalongan, Kecamatan Patikraja, Kabupaten Banyumas. Permasalahan yang sering dihadapi peternak di KTT Terus Jaya adalah sulitnya memperoleh pakan berkualitas terutama saat musim kemarau. Pengolahan limbah pertanian menggunakan teknologi amofer menggunakan starter Mikroorganisme Lokal (MOL) merupakan salah satu solusi untuk mengatasi permasalahan tersebut. Kegiatan pengabdian ini bertujuan untuk meningkatkan keterampilan peternak dalam membuat starter MOL dan amofer limbah pertanian. Metode yang digunakan adalah sosialisasi dan demonstrasi. Pengukuran peningkatan keterampilan dilakukan dengan menggunakan pretest dan Post-test. Hasil yang diperoleh adalah adanya peningkatan keterampilan peternak dalam memanfaatkan limbah di sekitar untuk pembuatan starter MOL berbahan dasar limbah idustri tepung tapioca (onggok) dan amofer jerami padi. Rata-rata skor Post-test yang diperoleh setelah diadakannya sosialisasi dan demonstrasi yaitu sebesar 79,5 menunjukan pemahaman dan keterampilan peternak sudah cukup baik.
Kandungan Lemak Kasar dan BETN Pada Amofer Jerami Padi Dengan Penambahan Starter MOL Asal Nasi Basi dan Onggok Wahyuddin, Akhmad; Fitria, Restuti; Hindratiningrum, Novita
Buletin Peternakan Tropis Vol. 5 No. 1 (2024)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/bpt.5.1.24-30

Abstract

The purpose of the study was to determine the crude fat content and nitrogrn-free extract (NFE) in the production of rice straw amofer using MOL (Local Microoganism) based on stale rice and cassava dregs, and to compare MOL starter based on stale rice and cassava dregs with EM4. The research was conducted using the Completely Randomised Design (CRD) method with 4 treatments and 5 replications. Treatments inthis study were: R0 (rice straw amofer without addition of strater); R1 (rice straw amofer using Molba NF1); R2 (rice straw amofer using Molba NF3); and R3 (rice straw amofer using EM4). The observed variables were crude fat and NFE.  The results showed that the addition of different types of starter had a significant effect on the crude fat and NFE in rice straw amofer (P<0.01). The addition of both MOL from waste and EM4 starter was able to increase crude fat content by 0,74% to 1,18% and was able to reduce NFE content by 1,75% to 4,24%. The conclusion of this study is that the addition of different starters in the production of rice straw amofer can increase the crude fat content and reduce NFE content. The use of MOL from stale rice and cassava dregs gave results for crude fat content and NFE content that were almost the same as EM4.   Key words: Amofer; MOL Starter; Crude Fat; Nitrogen-Free Extract   ABSTRAK Tujuan penelitian yaitu mengetahui kandungan lemak kasar dan BETN (bahan ekstrak tanpa nitrogen) pada pembuatan amofer jerami padi. Penelitian dilakukan menggunakan metode Rancangan Acak Lengkap (RAL) dengan 4 kali perlakuan dan 5 kali ulangan. Perlakuan antara lain: R0 (amofer jerami padi tanpa penambahan starter); R1 (amofer jerami padi menggunakan Molba NF1); R2 (amofer jerami padi menggunakan Molba NF3); dan R3 (amofer jerami padi menggunakan EM4). Variabel penelitian adalah kandungan lemak kasar dan BETN. Hasil penelitian menunjukkan  penambahan berbagai jenis starter yang berbeda berpengaruh sangat nyata terhadap kandungan lemak kasar dan BETN pada amofer jerami padi (P<0,01). Penamabahan starter baik MOL asal limbah dan EM4 mampu meningkatkan kandungan lemak kasar sebesar 0,74% sampai 1,18% serta mampu menurunkan kandungan BETN sebesar 1,75% sampai 4,24%. Kesimpulan dari penelitian ini bahwa perlakuan penambahan berbagai starter pada pembuatan amofer jerami padi mampu meningkatkan kandungan lemak kasar dan menurunkan kandungan BETN. Penggunaan starter MOL asal nasi basi dan onggok diperoleh hasil kandungan lemak kasar dan BETN hampir sama dengan EM4.   Kata kunci: Amofer, Starter MOL, Lemak Kasar, Bahan Ekstrak tanpa Nitrogen
Nutritional Quality of Amofer (Ammonia Fermentation) Corn Straw Using EM4 and M21 Decomposer Fitria, Restuti; Luthfi, Subhanul Arafat Chayatul; Hindratiningrum, Novita
Jurnal Sains dan Teknologi Peternakan Vol 5 No 1 (2023): Jurnal Sains dan Teknologi Peternakan
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/jstp.v5i1.3160

Abstract

This study aimed to identify the best starter levels of commercial cultures and incubation time on crude protein (CP), crude fiber (CF), extract ether (EE), and nitrogen-free extract (NFE) of amofer corn straw. This research used a completely randomized design with ten treatments and four replications. The treatments were the additions of EM4 (EM) and M21 decomposer (MD) at different levels (control/without amofer; 0.04 % EM; 0.06 % EM; 0.04 % MD; and 0.06 % MD) and incubation times (4 weeks and 2 weeks). The obtained data were analyzed by analysis of variance (ANOVA), then continued with the Duncan Multiple Range Test (DMRT). The result showed that the use of commercial starters with different fermentation times could increase the CP and NFE levels while reducing the EE and CF of amofer corn straw. The most effective treatment was the addition of 0,04 % EM and 2 weeks incubation which contained CP of 5.9415 %, CF of 37.8545 %, EE of 4.5183 %, and NFE of 32.4245 %.
Suplementasi Heit-Chrose pada Pakan Sapi Perah Pre-Partum Ditinjau dari Profil Darah dan Recovery Bobot Tubuh Post-Partum Caribu Hadi Prayitno; Restuti Fitria; Muhamad Samsi
Jurnal Agripet Vol 14, No 2 (2014): Volume 14, No. 2, Oktober 2014
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v14i2.1872

Abstract

(Pre-partum supplementation of heit-chrose on post-partum blood profile and recovery of body weight of dairy cows)ABSTRACT. This study aimed to determine the profile of blood, metabolic enzymes and recovery of body weight in dairy cows fed diet supplemented with Heit-CHrose (Feed supplement containing saponins, allicin, organic mineral: Se, Cr, and Zn). The experiments were conducted with 4 treatments, R0 : control diet (CP 13,79% and TDN 67,2%), R1 : R0 supplemented with organic minerals (Cr 1,5 ppm; Se 0,3 ppm; Zn-Lyzinat 40 ppm), R2 : Ro supplemented with Heit-CHrose are given in the component feeding, and R3 : Ro supplemented with Heit-CHrose are given in total mixed ration (TMR). Data were tested using analysis of variance with a completely randomized design using four replications, and HSD test. The results showed that the supplementation of Heit-CHrose to the diet of dairy cows gave significant effect (P 0.01) on blood glucose and recovery of body weight, but had no effect (P 0.05) on hemoglobin, blood urea nitrogen (BUN), total protein and blood cholesterol and metabolic enzyme (alkali phospatase). Supplementation of Heit Chrose in dairy cows diet with component feeding and TMR increases blood glucose and recovery of body weight.
The Nutrition Quality of Cassava Leaf Silage with Different Fermentation Lengths Hindratiningrum, Novita; Fitria, Restuti; Santosa, Setya Agus
ANIMAL PRODUCTION Vol. 25 No. 3 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.3.222

Abstract

This study aimed to observe the nutrition contents (crude protein/CP, crude fiber/CF, crude fat/CFat, and nitrogen-free extract/NFE) of cassava leaf silage using rice bran and starter EM-4 as the source of carbohydrates, with different fermentation lengths. The Completely Randomized Design was applied to four treatments (fermentation lengths) and five replicates. The treatments were P0 = 0-day fermentation, P1 = 7-day fermentation, P2 = 14-day fermentation, and P3 = 21-day fermentation. The obtained data were subjected to Analysis of Variance (ANOVA) and continued with Duncan’s Multiple Range Test (DMRT). The results showed that the fermentation lengths significantly affected the nutrient content of cassava leaf silage. The post hoc DMRT indicated different levels of CP, CFat, and CF across treatments, while R0 and R1 shared equal NFE. The difference and similarity of nutrient quality across treatments were due to different phases in fermentation. Day 0 through day 7 was the lag phase, and day 14 was the exponential phase which would decline until day 21 when it reached the stationary phase. Conclusively, 14-day fermentation was the best duration to make cassava leaf silage.
TOTAL BAKTERI SELULOLITIK DAN KEMAMPUAN HIDROLISIS PADA STARTER MIKROORGANISME LOKAL BERBASIS LIMBAH Fitria, Restuti; Hindratiningrum, Novita; Rayhan, Muhammad
STOCK Peternakan Vol 6, No 2 (2024): Stock Peternakan
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/sptr.v6i2.1551

Abstract

Mikoorganisme lokal merupakan mikroorganisme yang dapat dimanfaatkan untuk perombakan bahan organik dalam proses fermentasi pakan dan dapat diperoleh dengan memanfaatkan limbah yang ada di sekitar antara lain limbah rumah tangga seperti nasi basi, limbah pasar seperti sampah sayur, maupun limbah industri pangan seperti ampas tahu dan onggok. Tujuan penelitian ini untuk menganalisis bakteri selulolitik (total bakteri dan kemampuan hidrolisis) pada starter MOL berbasis limbah. Metode yang digunakan adalah metode eksperimental dengan 4 perlakuan yaitu starter MOL berbasis nasi basi (NF1), ampas tahu (NF2), onggok (NF3), dan sampah sayur (NF4). Variabel yang diukur adalah total bakteri dan kemampuan hidrolisis. Hasil penelitian kemudian dianalisis secara deskriptif kuantitatif. Hasil penelitian diperoleh baik pada perlakuan NF1, NF2, NF3, dan NF4 terkandung bakteri selulolitik dan total bakteri selulolitik dari tertinggi sampai terendah secara berturut-turut yaitu pada perlakuan NF1, NF2, NF3, dan NF4. Selain itu, diperoleh selulolitik isolat terpilih pada NF1, NF2, dan NF4 mampu membentuk zona hidrolisis sedangkan pada NF3 tidak ditemukan selulolitik isolat yang mampu membentuk zona hidrolisis Kesimpulan yang diperoleh adalah total bakteri selulolitik tertinggi diperoleh pada NF1 (3,0 x104 CFU/ml). Namun, kemampuan dalam menghidrolisis selulosa paling baik diperoleh pada selulolitik isolat terpilih pada perlakuan NF4 (1,92±0,13 cm).
Crude Protein and Fiber of Rice Straw Amofer plus Local Microorganims Based on Tomato Waste and Glucose Source: Protein dan Serat Kasar Amofer Jerami Padi yang Ditambah Mikroorganisme Lokal Berbasis Limbah Tomat dan Sumber Glukosa Handoyo, Erik; Hindratiningrum, Novita; Luthfi, SAC.; Fitria, Restuti
Buletin Peternakan Tropis Vol. 6 No. 1 (2025)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/bpt.6.1.72-80

Abstract

By adding tomato waste MOL starter, the purpose of this study is to ascertain the crude protein and crude fiber content of rice straw amofer which is added with different sources of glucose (brown sugar and molasses) and compare it with EM-4.  A Complete Randomized Design (CRD) with four treatments and repeated five times was used in the study.  The treatmens applied were P0: control was the treatment that was used (without adding starter); P1: incorporating brown sugar and tomato waste MOL starting; P2: adding tomato waste MOL starter with the addition of molasses, P3: adding EM4. The data obtained were analyzed by analysis of variance and followed by Duncan's multiple region test (DMRT) if there were differences in treatment.  The variable observed in this study were crude protein and crude fiber. The results showed that the treatment had a significant effect (P<0.05) on the crude protein and crude fiber content. Further DMRT tests showed the highest crude protein and the lowest crude fiber was P0 and if it was compared to other treatments significantly different (P<0.05).  The conclusion is that the addition of tomato MOL and EM-4 starters has not been able to increase crude protein levels and reduce crude fiber due to the limited energy contained in the substrate.
Identification of Lactic Acid Bacteria in Tapioca Waste Based Local Microorganism Starter with Different Molasses Addition: Identifikasi Bakteri Asam Laktat dari Starter Mikroorganisme Lokal Asal Onggok dengan Penambahan Molases yang Berbeda Fitria, Restuti; Hindratiningrum, Novita; Falah, Muhammad
Buletin Peternakan Tropis Vol. 6 No. 1 (2025)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/bpt.6.1.33-41

Abstract

The fermentation process requires a starter as a source of microorganisms. One of the microorganisms involved in the fermentation process is Lactic Acid Bacteria (LAB). Utilizing organic waste such as tapioca waste as a source of local microorganisms (MOL) for feed fermentation starter can not only improve feed quality, but also reduce the negative impact of poorly managed industrial waste on the environment. In addition, the utilization of molasses as a carbohydrate source in the preparation of starters can be done because it doesn’t compete with food. The aim of the study was identify LAB starter of tapioca waste with the addition of molasses at different levels. This research was conducted using descriptive qualitative and quantitative methods. There were 3 treatments, namely tapioca waste MOL starter with molasses addition of 20%(A), 30%(B), and 40%(C) respectively. The research variables observed were pH, morphological characteristics and total of LAB. The results showed that the pH produces ranged from2,45 to 2,63 and the average size of bacteria derived from all treatments was punctiform with white color, circular configuration, flat elevation, and entire margin. Based on gram straining, it was found that the bacteria growing in all treatments were gram-positive, non-spore-forming, and rod-shaped bacteria. The highest total LAB was obtained in treatment A, which was 50,4x105 CFU/ml. The conclusion is that based on the pH produces, the quality of MOL starter from each treatment is very good and the presence of LAB in all treatments with morphological characteristics of cells that are almost the same and show morphological characteristics of Lactobacillus.