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All Journal HAYATI Journal of Biosciences Jurnal Biologi Udayana Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Sinergitas PkM & CSR FaST : Jurnal Sains dan Teknologi Jurnal Ilmu Kefarmasian Indonesia Kumawula: Jurnal Pengabdian Kepada Masyarakat Prosiding Konferensi Nasional PKM-CSR
Sugata, Marcelia
Universitas Pelita Harapan
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health Social Sciences

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Protein Profile and Decolorization Potential of Copper-Resistant Klebsiella pneumonia CKJ 500 2.1.2 in Response to Textile Dyes and Copper Irawati, Wahyu; Pinontoan, Reinhard; Marcellie, Jessica; Valiant, Andreas; Sugata, Marcelia
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.584-595

Abstract

Heavy metals and synthetic dyes are major environmental pollutants, particularly in industrial effluents. The development of effective and safe bioremediation strategies to mitigate their ecological impact is therefore critical. In this study, the copper-resistant bacterium Klebsiella pneumoniae CKJ 500 2.1.2 was investigated for its capacity to decolorize textile dyes—specifically malachite green—and its associated enzymatic activities were characterized. Bacterial resistance was assessed using Luria–Bertani agar containing varying concentrations of copper and dyes. Decolorization efficiency was evaluated spectrophotometrically, protein expression was analyzed using SDS-PAGE, and dye degradation products were identified using gas chromatography–mass spectrometry (GC-MS). The strain exhibited high tolerance to both copper and dyes, achieving 99.4% decolorization of malachite green and 81% for Congo red. The presence of copper inhibited the decolorization of most dyes, except malachite green and methylene blue. SDS-PAGE analysis identified three key enzymes: laccase (~60 kDa), manganese peroxidase (~39 kDa), and azoreductase (~22 kDa). GC-MS revealed both toxic and non-toxic degradation intermediates, indicating partial detoxification. These findings highlight the potential of K. pneumoniae CKJ 500 2.1.2 for bioremediation of dye-contaminated effluents. However, further research is required to elucidate the complete enzymatic pathways involved and to ensure environmentally safe dye degradation.
Co-Authors Abigail Christy, Suawa Natania Adhiwijaya, Ruben Patrick Agustina Ika Susanti Anasthasia Nathania Widjaja Ariela Samantha Ariela Samantha Callista Levina Wahyudi Candra, Tasya Mariska Celeste Abigail Thanos Chelsea Valeria Simamora Christy, Suawa Natania Abigail Daniel Elia Firmansyah Daniel, Juan Dela Rosa Denny Juvi Erlangga Kodrat Febriani, Enjelia Ferren Chrislin Figo, Figo Firmansyah, Daniel Elia JAP, LUCY Jessica Novia Jessica Novia Jessica Trisina Juandy Jo Kandou, Abigail Sabrina Karnelasatri Karnelasatri Kodrat, Erlangga Litanto, Vivian Marbun, Kristin Talia Marcellie, Jessica Meiryanti Layarda Octaviana, Cindy Pongajow, Billy Yosua Costantin Reinhard Pinontoan Riskianto Riskianto Ruben Patrick Adhiwijaya Samantha, Ariela Sanjaya, Astia Simamora, Chelsea Valeria Soewono, Effendi Sophiano, Theophilus Suawa Natania Abigail Christy Sunardi, Jessica Suphandi, Michell Tan Tjie Jan, Tan Tjie Thanos, Celeste Abigail Timothy, Michael Valiant, Andreas Victor, Hans Vidian, Valerie Vivian Litanto WAHYU IRAWATI Wahyudi, Callista Levina Watumbara, Medista Lisa Widjaja, Anasthasia Nathania Yohan Aditya Manik