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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Sains Dasar Jurnal Riset Kimia JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya Chimica et Natura Acta Acta Pharmaciae Indonesia : Acta Pharm Indo Jurnal Biologi Tropis Jurnal Kartika Kimia Jurnal Inovasi Teknik Kimia Berkala Penelitian Hayati Prosiding Konferensi Nasional PKM-CSR Jurnal Pengabdian Isola
Lela Lailatul Khumaisah
Universitas Muhammadiyah Sukabumi
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Uji Efektivitas Formula E-Liquid Minyak Sereh Wangi (Cymbopogon nardus L.) sebagai Repelan terhadap Aedes aegypti Aji Achmad Saputra; Dikdik Mulyadi; Lela Lailatul Khumaisah
Chimica et Natura Acta Vol 8, No 3 (2020)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v8.n3.26257

Abstract

Formulasi e-liquid berbahan minyak sereh wangi (Cymbopogon nardus L.), analisis minyak sereh wangi dan e-liquid dengan GC-MS dan uji aktivitas e-liquid sebagai repelan terhadap nyamuk Aedes aegypti telah dilakukan. Minyak sereh wangi digunakan sebagai bahan tambahan e-liquid karena mengandung senyawa minyak atsiri berupa sitronelol, sitronelal dan geraniol yang memiliki kemampuan sebagai repelan terhadap nyamuk, sehingga diharapkan asap yang keluar dari e-liquid dapat menolak nyamuk. Konsentrasi minyak sereh wangi yang dibuat untuk formulasi e-liquid terdiri dari 0,25; 2,5; 5 dan 10%. Hasil uji efektivitas repelan e-liquid selama satu jam pengamatan diperoleh nilai 100% pada konsentrasi 10%.  Hasil analisis GC-MS diketahui bahwa pada minyak sereh wangi maupun e-liquid terdapat 3 senyawa di atas sehingga berpotensi sebagai repelan (penolak nyamuk). Uji aktivitas e-liquid sebagai repelan terhadap nyamuk Aedes aegypti dilakukan dengan variasi konsentrasi 0,25; 2,50; 5 dan 10% selama 24 jam pengamatan. Persentase kematian rata-rata nyamuk pada konsentrasi tertinggi sebesar 33% dengan nilai LC50 13% dan nilai LT50 62 menit.
Phytochemical Screening and Antibacterial Test of Leaf Extract of Canar Susu (Smilax macrocarpa Blume) Against Eschercihia coli, Pseudomonas aeruginosa, and Staphylococcus epidermidis Lela Lailatul Khumaisah; Vina Juliana Anggraeni; Muhamad Salman Fareza
Acta Pharmaciae Indonesia : Acta Pharm Indo Vol 7 No 1 (2019): Acta Pharmaciae Indonesia : Acta Pharm Indo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (564.226 KB) | DOI: 10.20884/1.api.2019.7.1.2451

Abstract

Smilax adalah salah satu genus Smilacaceae yang banyak dimanfaatkan sebagai tumbuhan obat karena mengandung berbagai metabolit sekunder dengan beberapa bioaktivitas, seperti anti-inflamasi, antirematik, analgentik, antioksidan, antikanker dan antibakteri. Spesies Smilax yang belum pernah dikaji dan hanya tumbuh di Indonesia adalah Smilax macrocarpa Blume (canar susu). Penelitian bertujuan untuk mengkaji karakteristik simplisia, kandungan fitokimia, beserta sifat toktsisitas dan antibakterinya dari ekstrak daun tumbuhan ini. Penelitian dilakukan dengan metode ekstraksi menggunakan teknik maserasi dengan pelarut metanol. Selanjutnya terhadap ekstrak S. macrocarpa Blume dilakukan karakteristik simplisia, uji toksisitas dengan metode BSLT, skrining fitokimia menurut metode Harborne, dan uji aktivitas antibakteri menggunakan metode mikrodilusi terhadap bakteri Escherihia coli, Pseudomonas aeruginosa, dan Staphylococcus epidermidis. Berdasarkan hasil yang diperoleh diketahui bahwa ekstrak metanol daun canar susu mengandung alkaloid, flavonoid, steroid, tanin, terpenoid, saponin dan glikosida. Kadar air, kadar abu, kadar abu tak larut asam, kadar sari air, dan kadar sari alkohol berturut-turut 8,74%; 3,60%; 0,11%; 19,01% dan 5,40%. Hasil toksisitas ekstrak diperoleh nilai LC50 sebesar 680,07 ppm. Pada uji aktivitas antibakteri terhadap E. coli memiliki nilai MIC 625 ppm, sedangkan pada P. aeruginosa dan S. epidermidis ATCC 12228 masing-masing 1.250 ppm. Adapun nilai MBC untuk E. coli, P. aeruginosa dan S. epidermidis ATCC 12228 masing-masing sebesar 5.000 ppm. Dari hasil ini canar susu tidak berpotensi sebagai antibakteri, tetapi bisa berpotensi sebagai biopestisida dilihat dari nilai toksisitasnya.
KOMPOSISI KIMIA DAN UJI AKTIVITAS ANTIBAKTERI MINYAK KEMANGI (Ocimum americanum L.) TERHADAP BAKTERI Escherichia coli, Shigella sonnei, DAN Salmonella enteritidis Asep Kadarohman; Gebi Dwiyanti; Yuni Anggraeni; Lela Lailatul Khumaisah
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/287

Abstract

The chemical composition and antibacterial activity of basil oil (Ocimum americanum L.) against Escherichia coli, Shigella sonnei and Salmonella enteritidis have been determined. Basil oils were isolated from leaves and stems of basil (Ocimum americanum L.) with percolation method and analyzed by FTIR and GC-MS spectrometry. The antibacterial activity of basil oils were obtained by Agar diffusion method with various concentration (v/v) 2, 4, 6, 8, and 10% with ethanol p.a. as negative control, thiamfenicol, and tetracycline 500 mg as positive control. The produces essential oils from leaves and stems of basil by percolation are 1.06 and 0.22%, respectively. There are 22 components of basil oil were identified, with a major component are citral (35.58%) and neral (29.56% ). Basil oil has not effectively against Escherichia coli and Shigella sonnei but effectively against Salmonella enteritidis at concentration 8 and 10%, with inhibition zone diameter are 10.25 and 10.93 mm respectively.
Antifungal Activity of Red Galangal Oil (Alpinia purpurata K. SCHUM) Against Malassezia furfur Hernandi Sujono; Senadi Budiman; Yusi Fudiesta; Ahmad Sahroni; Jasmansyah Jasmansyah; Lela Lailatul Khumaisah
Jurnal Kartika Kimia Vol 2 No 2 (2019): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, Jenderal Achmad Yani University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (494.865 KB) | DOI: 10.26874/jkk.v2i2.40

Abstract

Plant red galangal (Alpinia purpurata K. Schum) is one of the many spices used as bio-pharmacy products which contain essential oil that is active as an antifungal and antibacterial agent. Isolation of volatile oil from red galangal rhizome (Alpinia purpurata K. Schum) uses the method of water and steam distillation so as to get the rendemen 0.1062%, the density 0.9524 g/mL and the index of refraction by 1.4862. Results from GC-MS (Gas Chromatography-Mass Spectrometry) retrieved 30 compounds contained in red galangal rhizome oil with 10 major compounds i.e. 1,8-cineole (40.92%), acetyl chavicol (10.33%), cis β-farnesene (6.91%), 1-caryophillene (6.32%), 1-β-bisabolene (3.37%), β-elemene (3.23%), α-pinene (3.20%), β-sesquiphellandrene (2.32%), β-pinene (2.21%), and germacrene-D (1.90%). The inhibition test was done by agar diffusion method and different variations of concentration (1, 2, 3 and 4%) compared with ketoconazole 2% as positive controls and dimethyl sulfoxide (DMSO) as negative controls with an incubation period of 2x24 hours. The test results show that the essential oil of red galangal rhizome (Alpinia purpurata K. Schum) can inhibit the growth of the fungus Malassezia furfur with the inhibitory power on concentrations1, 2, 3 and 4% were 7.15 mm, 13.87 mm, 16.05 mm and 20.05 mm, respectively. As for comparison, ketoconazole 2% was used as positive (+) control that produces inhibitory zone 27.20 mm and dimethyl sulfoxide (DMSO) as negative control (-) which does not produce inhibitory zones. Keywords: Essential oil, red galangal rhizome (Alpinia purpurata K. Schum), Malassezia furfur
Pemanfaatan Tanaman Minyak Atsiri Sebagai Pengendali Hama Tikus Padi (Biopestisida) Di Kabupaten Sukabumi Lela Lailatul Khumaisah; Lela Mukmilah Yuningsih; Asep Kadarohman
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 2 (2019): Peran Perguruan Tinggi dan Dunia Usaha dalam Mempersiapkan Masyarakat Menghadapi Era I
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.177 KB) | DOI: 10.37695/pkmcsr.v2i0.450

Abstract

The chemical content in essential oils have activities on microbes, biopesticides, pathogenic vector insects in humans and animals, including Mentha arvensis and Mentha piperita (mint plants) which have antifertility and antibacterial effects, Vetiveria zizanoides (vetiver root) as insects repellent, Cymbopogon nardus (lemongrass) has an activity against plant pathogenic bacteria and antifungals, and Rosmarinus officinalis (rosemary) has locomotor activity of mice and antibacterial. Essential oils are effective against target organisms, compatible, safe and non-toxic, so they have enormous potential to be developed as biopesticides in controlling rice pests. On the other hand, pests and diseases are problems that always disturb rice farmers, including in Sukaresmi and Kebon Pedes villages, Sukabumi. Therefore, the use of essential oil plants has been carried out to control rice mouse pests. This has been started from the preparation of seeds to process of planting the four types of essential oils (mint, lemongrass, and rosemary) in the fields as intercrops and the procurement of essential oil distillation sets. Based on observations, the growth and development of essential oil plants as intercropping plants on rice runs normally and its impact on rice pests, especially in mice, is quite significant with not found rats around the planting area. Keywords: Mint, lemongrass, rosemary, essential oil, biopesticide
Aktivitas Antibakteri Nanokomposit TiO2/Cu dan TiO2/CuO terhadap Bakteri Bacillus cereus Ine Nuhaeroh; Devi Indah Anwar; Lela Lailatul Khumaisah
Jurnal Sains Dasar Vol 11, No 2 (2022): October 2022
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v11i2.53247

Abstract

Perkembangan nanoteknologi sebagai nanomaterial telah banyak dimanfaatkan salah satunya dalam bentuk nanokomposit. Nanokomposit banyak diaplikasikan sebagai fotokatalis untuk mendegradasi logam berat dan dewasa ini banyak penelitian mengenai manfaat nanokomposit sebagai bahan antibakteri. Penelitian ini bertujuan menganalisis aktivitas antibakteri dari nanokomposit TiO2/Cu dan TiO2/CuO hasil sintesis terhadap Bacillus cereus. Metode yang digunakan dalam melakukan sintesis nanokomposit adalah metode impregnasi basah. CuSO4 merupakan prekursor yang digunakan untuk mensinteis TiO2/Cu sedangkan TiO2/CuO menggunakan CuCl2. Suhu kalsinasi yang digunakan adalah 6000C. Material TiO2/Cu yang dihasilkan berukuran 29.01 nm sedangkan TiO2/CuO 27.64 nm. Uji karaterisasi X-Ray Diffraction (XRD) dilakukan untuk mengetahui pembentukkan nanokomposit TiO2/Cu dan TiO2/CuO. Puncak difraksi pada sudut 2θ TiO2 yaitu 25.33o, CuO 38.70, Cu 43.60 dan menandakkan nanokomposit berhasil terbentuk. Hasil pengujian aktivitas antibakteri terhadap Bacillus cereus dengan menggunakan metode difusi cakram menunjukkan TiO2/Cu memiliki zona hambat bakteri 8.10 mm dan TiO2/CuO 11.40 mm dan termasuk ke dalam antibakteri kuat sedangkan nanopartikel CuO merupakan antibakteri sedang dengan nilai sebesar 7.85 mm. Hasil ini menunjukkan nanokomposit memiliki daya hambat bakteri lebih tinggi dibandingkan nanopartikelnya.
Antibacterial Activity against Gram-Positive and Gram-Negative Bacteria and Biolarvicide to Aedes aegypti from Cocok Bubu (Elatostema rostratum (Blume) Hassk) Leaves Extract Lela Lailatul Khumaisah; Rika Ayuningtias; Salih Muharam; Reni Mulyani; Luthfi Awaliah
Jurnal Riset Kimia Vol. 14 No. 2 (2023): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v14i2.613

Abstract

Tropical diseases are infectious diseases that commonly occur in tropical climates. Infectious diseases are caused by bacteria, viruses, parasites, and are transmitted by several vectors. There are 8 neglected tropical diseases (NTDs) that have spread in Indonesia. Therefore, massive efforts are needed to overcome this disease. Active plant substances have long been popular in treating various diseases. Cocok Bubu (Elatostema rostratum (Blume) Hassk) is an endemic plant of Indonesia. Ethnobotanical studies of this plant are used to treat fever and diarrhea. But, there are no pharmacological studies on previous research because this species belongs to a limited distribution plant. However, Elatostema has a secondary metabolite with various pharmacological activities. This study aims to determine the activity of antibacterial and biolarvicides from Cocok bubu leaf extract. The extraction using the maceration method with acetone, phytochemicals screening, toxicity test using BSLT, antibacterial test with disc diffusion to Escherichia coli, Staphylococcus aureus, Streptococcus mutant, and Streptococcus sanguinis, and biolarvicide activity against Aedes aegypti. Based on the results, Cocok bubu leaf extract contains flavonoids, alkaloids, saponin, tannin, terpenoids, and steroids with a toxicity of 758.45 ppm. From antibacterial activity results, Cocok bubu exctract had the best inhibition zone against Staphylococcus aureus (10 – 18 mm). According to the biolarvicide test, it is effective as a biolarvicide to Aedes aegypti with LC50 51.099 ppm and LT50 of 5 h 43 min. This study shows that Cocok bubu was more effective for treating and preventing NTDs in Indonesia, especially dengue and chikungunya fever in the future.
POTENSI LIMBAH CANGKANG BIJI KARET DAN SEKAM PADI UNTUK BAHAN DASAR GRAFENA OKSIDA TEREDUKSI (rGO) SEBAGAI ADSORBEN PENJERNIH MINYAK Devi Indah Anwar; Lela Lailatul Khumaisah; eri rizki hariyadi
Jurnal Inovasi Teknik Kimia Vol 9, No 2 (2024)
Publisher : Fakultas Teknik Universitas Wahid Hasyim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31942/inteka.v9i2.9716

Abstract

Cangkang biji karet (CBK) dan sekam padi (SP) merupakan limbah biomassa yang mengandung bahan lignoselulosa seperti selulosa, hemiselulosa dan lignin. Dengan adanya kandungan senyawa tersebut cangkang biji karet dan sekam padi berpotensi digunakan sebagai bahan baku dalam sintesis grafena oksida tereduksi (rGO). Grafena oksida tereduksi merupakan senyawa yang dihasilkan melalui reduksi atau penghilangan gugus fungsi yang mengandung oksigen dari grafena oksida. Pada penelitian ini, grafena oksida tereduksi disintesis menggunakan metode Hummer termodifikasi yaitu mengganti NaNO3 dengan H3PO4 agar tidak dihasilkan gas beracun NO2 dan N2O4 serta menggunakan agen pereduksi berupa Zn. Hasil sintesis dikarakterisasi dengan menggunakan XRD dan FTIR, serta dipalikasikan sebagai adsorben untuk menjernihkan minyak. Berdasarkan hasil karakterisasi XRD, rGO CBK dan SP masing-masing memiliki puncak 2θ = 23,5° dan 21,2o dengan kristalinitas 17 dan 13%. Karakterisasi FTIR menunjukkan perubahan struktur dengan berkurangnya atom O dan hilangnya gugus OH. Adsorpsi untuk menjernihkan minyak menunjukkah persen penurunan sebesar 86% untuk rGO CBK dan 74% untuk rGO SP.
Biolarvicide activity of extract and essential oil of culantro (Eryngium foetidum L.) on Culex quinquefasciatus larvae in silico and in vitro Winarti, Sri Ayu; Khumaisah, Lela Lailatul; Anwar, Devi Indah
Acta Pharmaciae Indonesia Vol 11 No 2 (2023): Acta Pharmaciae Indonesia: Acta Pharm Indo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.api.2023.11.2.8925

Abstract

Background: Filariasis, a priority tropical disease in Indonesia, is transmitted by the Culex quinquefasciatus mosquito. The use of conventional synthetic larvicides can lead to environmental and health issues, including poisoning and resistance in target insect populations. Objective: This study explores the utilization of biolarvicides derived from culantro (Eryngium foetidum) to mitigate these adverse efects, focusing on analyzing the components and evaluating the larvicidal eficacy of both the extract and essential oil of E. foetidum. Method: This study employed a molecular docking approach to examine in silico biolarvicidal activity against the odorant binding protein (OBP) receptor and conducted in vitro experiments on Cx. quinquefasciatus larvae using varying concentrations of E. foetidum extract (100, 250, and 500 ppm) and essential oil (10, 50, and 100 ppm). Results: The in silico study identified hynokiflavone and longifenaldehyde as the compounds with the most potent activity, demonstrating binding a inities of -10.2 and -9.5 kcal/mol, respectively. The in vitro assays revealed that the E. foetidum extract achieved 75% larval mortality at an LC50 of 78.59 ppm, while the essential oil resulted in 88% mortality with an LC50 of 10.13 ppm. Conclusion:The extract and essential oil of E. foetidum exhibit significant biolarvicidal activity against Culex quinquefasciatus, offering promising plant-based alternatives to traditional larvicides, with implications for safer and more sustainable vector control strategies.
SINTESIS GRAFENA OKSIDA TEREDUKSI BERBAHAN DASAR CANGKANG BIJI KARET DAN SEKAM PADI SERTA KOMPOSITNYA DENGAN METODE HUMMER TERMODIFIKASI Anwar, Devi Indah; Khumaisah, Lela Lailatul; Haryadi, Eri Rizki
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 12 No 1 (2024): Cakra Kimia (Indonesia E-Journal of Applied Chemistry)
Publisher : Graduate Program of Applied Chemistry, Udayana University, Bali-INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK: Grafena merupakan material dua dimensi monoatomik dari satu lapis grafit dengan ketebalan sekitar satu atom karbon yang memiliki transparansi optik hingga 97.7%. Kegunaan grafena diantaranya adalah sebagai antibakteri, adsorben, biosensor, dan kapasitor. Metode sintesis grafena sudah banyak dilakukan diantaranya menggunakan metode Chemical Vapor Decomposition (CVD), micromechanical ekfoliation (ME) dan metode Hummer. Namun beberapa metode tersebut memiliki banyak kekurangan yakni waktu yang diperlukan cukup lama, biaya yang relatif tinggi, serta dapat menghasilkan gas beracun seperti NO2 dan N2O4. Alternatif metode yang dapat digunakan adalah metode Hummer termodifikasi dengan mengganti NaNO3 dengan H3PO4 yang akan menghasilkan material grafena berupa grafena oksida tereduksi (rGO). Adapun bahan dasar sintesis grafena yang digunakan pada penelitian ini berasal dari cangkang biji karet (CBK) dan sekam padi (SP). Hal ini dikarenakan CBK mengandung 48.64% selulosa dan 21.60% lignin. Sedangkan 38% selulosa, 18% hemiselulosa, 22% lignin dan 19% silika oksida terkandung pada sekam padi. Tujuan penelitian ini adalah untuk mensintesis grafena oksida tereduksi (rGO) dari cangkang biji karet, sekam padi dan komposit keduanya (KCS), serta mengkarakterisasi rGO yang terbentuk menggunakan X-Ray Diffraction (XRD) dan FTIR. Berdasarkan hasil analisis XRD diperoleh sudut difraksi 2? dari rGO CBK, SP, dan KCS berturut-turut 23.5, 21.2, dan 24.3° dengan kristalinitas sebesar 17%, 13%, dan 10%. Hasil tersebut menunjukan sifat material yang amorf. Pada karakterisasi FTIR menunjukkan perubahan struktur pada rGO setelah direduksi dengan berkurangnya atom O serta hilangnya gugus O-H yang terdapat pada rGO. ABSTRACT: Graphene is a monoatomic two-dimensional material made of one layer of graphite with a thickness of about one carbon atom which has an optical transparency of up to 97.7%. The uses of graphene itself include being an antibacterial, adsorbent, biosensor, and capacitor. Many graphene synthesis methods have been carried out, including using the Chemical Vapor Decomposition (CVD) method, micromechanical exfoliation (ME) and the Hummer method. However, some of these methods have many drawbacks, namely the time required is quite long, the cost is relatively high and can produce toxic gases such as NO2 and N2O4. An alternative method that can be used is the modified Hummer method by replacing NaNO3 with H3PO4 and producing graphene material which is formed in the form of reduced graphene oxide (rGO), because it has a very good content. The content in the rubber seed shell is 48.64% cellulose and 21.60% lignin. Meanwhile, 38% cellulose, 18% hemicellulose, 22% lignin and 19% silica oxide are contained in rice husks. The aims of this study were to synthesize graphene from rubber seed shells, rice husks and their composite (KCS), as well as to characterize the rGO formed using X-Ray Diffraction (XRD) and FTIR. Based on the results of XRD analysis, the diffraction angle of 2? from rGO CBK, SP, and KCS was 23.5, 21.2, and 24.3° respectively with crystallinities of 17%, 13%, and 10%. These results indicate the amorphous nature of the material. In FTIR characterization, it shows changes in the structure of rGO after being reduced by reducing O atoms and the loss of O-H groups present in rGO
Co-Authors Ahmad Sahroni Aji Achmad Saputra Anggraeni, Vina Juliana Asep Kadarohman Asep Kadarohman Devi Indah Anwar Dikdik Mulyadi eri rizki hariyadi Gebi Dwiyanti Haryadi, Eri Rizki Hayati, Mila Nurmala Hernandi Sujono Ine Nuhaeroh Jasmansyah Jasmansyah Juliansyah, Aris Lela Mukmilah Yuningsih Luthfi Awaliah Muhamad Salman Fareza Reni Mulyani Rika Ayuningtias Salih Muharam Senadi Budiman Suardi, Cici Winarti, Sri Ayu Yuni Anggraeni Yuniarti, Nia Yusi Fudiesta