Claim Missing Document
Check
Articles

Found 27 Documents
Search

Karakteristik Morfologi dan Fisiologi Isolat Pantoea stewartii subsp. stewartii pada Jagung Yulfi Desi; Trimurti Habazar; Agustian Agustian; Ujang Khairul; Syamsuwirman Syamsuwirman; Prima Novia
Jurnal Fitopatologi Indonesia Vol 10 No 2 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.074 KB) | DOI: 10.14692/jfi.10.2.45

Abstract

Pantoea stewartii subsp. stewartii is the pathogen causing stewart’s wilt disease in maize. The occurrence of the disease was recently reported in Indonesia with limited information. Research was conducted to characterize several isolates of P. stewartii subsp. stewartii isolated from maize plants collected from 6 districts in West Sumatra. Leaves showing yellow stripes symptoms along the vein was ground and suspeneded then the bacteria was isolated and incubated for 3 × 24 h at room temperature in nutrient glucose agar (NGA). Pure bacterial colonies gained from this medium was used for characterization. Assessment of bacterial characters was conducted based on morphological assay, physiological assay, biological assay (hypersensitivity, and pathogenicity) followed by polymerase chain reaction (PCR)-based identification. Unique characters of the family Enterobacteriaceae was observed on 7 isolates based on morphological and physiological characters, and only 4 isolates (PR1, PP, ST1, LA) gave positive reaction on hypersensitivity and pathogenicity test. Further molecular identification confirmed 3 out of 7 isolates had high homology to Pantoea stewartii, i.e. Pantoea stewartii strain R1-104, R1-132, and ATCC 29923 for isolates LA, PR1, and PR2, respectively.
Formulasi Bakteri Endofit Akar Kedelai untuk Pengendalian Pustul Bakteri Trimurti Habazar; Zurai Resti; Yulmira Yanti; Sutoyo Sutoyo; Imelda Imelda
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.147 KB) | DOI: 10.14692/jfi.11.2.51

Abstract

Two isolates of bacterial endophyte from soybean root were found to be effective to control bacterial pustule caused by Xanthomonas axonopodis pv. glycines. Formulation of the bacterial isolates is required to maintain the effectivity of this bacterial isolates during storage, transportation and application. The aim of this research was to obtain the best carrier for formulation to maintain the effectivity of bacterial endophyte in storage to control bacterial pustule on soybean. Three kind of carrier agent was evaluated for formulation of bacterial endophyte i.e. peat soil, tapioca flour and coconut water + 1% palm oil. Each carrier agent was combined with 5 treatment of storage time i.e. 0, 1, 3, 5 and 7 weeks. Soybean plants was inoculated by. X. axonopodis pv. glycines 2 weeks after planting by rubbing bacterial suspension (106 cel mL-1) on lower surface of leaves. The results showed that all formulas of bacterial endophyte were able to suppress the bacterial pustule on soybean. The best formulations were bacterial endophyte in peat soil stored for 1 and 7 weeks (effectivity rate 79.85% and 77.02%) and coconut water + palm oil and storaged for 3 weeks (effectivity rate 77.46%).
Detection and Sequence Diversity of Begomovirus Associated with Yellow Leaf Curl Disease of Pepper (Capsicum annuum) in West Sumatra, Indonesia JUMSU TRISNO; SRI HENDRASTUTI HIDAYAT; TRIMURTI HABAZAR; ISHAK MANTI; . JAMSARI
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.352 KB) | DOI: 10.5454/mi.3.2.2

Abstract

Yellow leaf curl disease of pepper has become an emerging important disease in West Sumatra since early 2000. Several attempts have been made, including disease survey and detection, in order to identify the causal agent of the disease. Pepper (Capsicum annuum) plants showing yellow leaf curl from West Sumatra were analyzed for presence of Begomovirus employing Polymerase Chain Reaction (PCR) with degenerate primers pAL1v 1978 and pARc 715. A DNA fragment of 1.6 kb was successfully amplified and subjected to direct sequencing. A stem loop region was found in the nucleotide sequence obtained, which contains the conserved nucleotide signature sequence TAATATTAC present in begomoviruses. Based on the stem loop region comparisons and phylogenetic analysis, the virus isolates from West Sumatra showed the closest relationship to Pepper yellow leaf curl Indonesia virus (PYLVIV) and Tomato yellow leaf curl Indonesia virus (TYLCIV). The sequence was different from other Asia Begomoviruses reported earlier. These isolates were divided into three groups which were tentatively called Pepper yellow leaf curl Indonesia virus-West Sumatra-[group 1], -[group 2] and -[group 3] {PYLCIV-WS-[group1], -[group2], and -[group3]}.
SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH Zurai Resti; Trimurti Habazar; Deddi Prima Putra; . Nasrun
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 2 (2013): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.607 KB) | DOI: 10.23960/j.hptt.213167-178

Abstract

Screening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012. Laboratory experiment consisted of three steps: (1) isolation of endophytic bacteria from healthy onion roots, (2)In planta /screening of endophytic isolates capable of reducing bacterial leaf blight disease, and (3) molecular identification of potential endophytic isolates. Treatments of in planta test were arranged in Completely Randomized Design. Collected isolates were tested for their capability in controlling bacterial leaf blight disease on shallot. The variables observed were disease incidence, disease severity, and shallot yield. The results showed that out of 82 isolates successfully isolated, 56 isolates (68.29%) were Gram positive, and 26 isolate (31.71%) were Gram negative. All isolates were HR negative and pathogenicity negative. Six endophytic isolates showed better performance in inducing resistance and increasing onion yield. Based on 16S rRNA sequence the six isolates were Bacillus cereus strain P14 , Bacillus cereus strain Se07, Bacillus sp H1, Bacillus sp SJ1 and Serratia marcescens strain PPM4.
Penapisan Isolat Rizobakteri dari Perakaran Tanaman Kedelai yang Sehat untuk Pengendalian Penyakit Pustul Bakteri (Xanthomonas axonopodis pv. glycines) Yulmira Yanti; Trimurti Habazar; Zurai Resti; Dewi Suhalita
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 1 (2013): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.092 KB) | DOI: 10.23960/j.hptt.11324-34

Abstract

Screening of indigenous rhizobacteria from healthy soybean root to control bacterial pustule (Xanthomonas axonopodis pv. glycines) using in planta technique. Plant growth promoting rhizobacteria are a group of bacteria that actively colonize plant roots, increase plant growth and control plant pathogens. The aim of this study was to obtain rhizobacteri isolates which have the ability to control bacterial pustule and increase growth and yield of soybean. This method based on in planta selection of enhanced competitive soil root-colonizing bacteria from soil samples of healthy soybean root at endemic area of bacterial pustule in Darmasraya District and Sijunjung District, West Sumatera. We characterized only the best rhizobacteri isolates which have ability to control bacterial pustule and to increase growth and yield of soybean. This type of characterization has possibility to find new, easy and cheap biocontrol organisms. Ten Rhizobacteri isolates were introduced via seed treatment (108 cfu/ml) and soil drench to 3 week old soybean seedling. Xanthomonas axonopodis pv. glycines were inoculated to one month old of soybean seedling. The effect of rhizobacteria on disease incidence, disease severity, plant growth and yield of soybean were evaluated. We have found that two selected rhizobacteri isolates from soybean (P12Rz2.1 and P14Rz1.1) were the best isolates in promoting growth and the of the soybean plants with the effectiveness 20.62 % and 20.47 %.
POTENSI TRIGONA SPP. SEBAGAI AGEN PENYEBAR BAKTERI RALSTONIA SOLANACEARUM PHYLOTIPE IV PENYEBAB PENYAKIT DARAH PADA TANAMAN PISANG . Mairawita; Trimurti Habazar; Ahsol Hasyim; Nasril Nasir
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 1 (2012): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.575 KB) | DOI: 10.23960/j.hptt.11292-101

Abstract

Banana blood disease (Blood Disease Bacteria, BDB) caused by Ralstonia solanacearum Phylotype IV is the most important banana disease in Indonesia. So far, information on the spread of disease by insects is very limited. The research was aimed to determine the role of insect as a disseminator of R. solanacearum Phylotype IV and to determine the amount of BDB inoculum carried by each individual insect. The experiment was conducted in May - September 2008. Samples of insects (adult insects, the young insects, larvae, eggs), nectar, and pollen were taken from a colony of Trigona spp. collected from BDB endemic area, Baso plateau (876 m asl) using purposive sampling method. Active adult insects were collected from the BDB infected banana flowers and healthy banana flowers. BDB on adult insects was isolated from the caput and abdomen, while for the young insects, larvae and pupae the isolation source were not differentiated. Each of the samples was rinsed, macerated, and cultured on medium containing Triphenyl Tetrazolium Chloride (TTC). BDB isolate characterization and identification were conducted through morphological, physiological, and pathogenicity tests. The parameter observed was the: population of BDB (cfu/ml) on each stage of insect development. The results showed that BDB can be isolated from the outside and the inside of the body of an adult, a young insect, pupa, larva also on pollen and nectar but it was not found in eggs. BDB population was higher in inside part fo the insect body in each phase of the development of the insect. From all phases, the BDB was higher in inner part of the body of adult insects which have visited infected banana flower.
INDUKSI KETAHANAN TANAMAN JAHE TERHADAP PENYAKIT LAYU Ralstonia solanacearum RAS 4 MENGGUNAKAN FUNGI MIKORIZA ARBUSKULAR (FMA) INDIGENUS Netty Suharti; Trimurti Habazar; Nasril Nasir; . Dachryanus; . Jamsari
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 1 (2011): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (585.073 KB) | DOI: 10.23960/j.hptt.111102-111

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum race 4 is a yield-limiting disease of ginger. A study to investigate the ability of indigenous Arbuscular Mycorrhizae Fungi (AMF) to reduce bacteria wilt disease has been done. This research was designed by using Randomized Block Design (RBD) at greenhouse experiments, using eight AMF isolates with six replication. As supporting data, population R. solanacearum race 4 after treatments was assesed. The results showed that aplication of indigenous AMF to ginger rhizome as seed treatment dicreased disease incidence, support ginger growth and production such as the seedling formation 150%, plant height 98.6%, the number of leaf 115,7%. As the result yield of ginger rhizome increased by 400 % and phyloplan 598,3%, respectively, compared to the control.
INTRODUKSI FORMULA RIZOBAKTERIA Bacillusthuringiensis pv. toumanoffi PADA TANAMAN KEDELAI UNTUK PENINGKATAN KETAHANAN TERHADAP PENYAKIT PUSTUL BAKTERI (Xanthomonas axonopodispvglycines) DI LAPANGAN Julio Eiffelt Rossaffelt Rumbiak; Trimurti Habazar; Yulmira Yanti
Jurnal Agroekoteknologi Vol 10, No 1 (2018)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v10i1.5462

Abstract

Bacillus thuringiensis srv.toumanoffi is an indigenous endophytic rizobacteria from healthy soybean rizosphere capable of controlling bacterial pustule disease (Xanthomonas axonopodispvglycines). Rhizobacteria needs to be formulated to remain effective in storage and application. The aimed of this research was to obtain a stable bacterial formula in controlling bacterial pustule disease in soybeans. This research was designed in factorial in a randomized group consisting of 13 treatments with 3 replications. The Treatment was a combination of carrier material (peat, tapioca and coconut water+1% palm oil) and storage time (0, 2, 4, and 6 weeks). Soybean seeds were introduced with each Bacillus thuringiensis srv. toumanoffi formula before planting. The results showed that the formula Bacillus thuringiensissrv. toumanofi was relatively stable in suppressing the incidence and severity of bacterial pustule disease in soybean leaf. The best formula for controlling bacterial pustules was Bacillus thuringiensis srv. toumanoffi in peat, coconut water + palm oil and tapioca each 2 weeks storage.
PENGEMBANGAN PERTANIAN ORGANIK MELALUI BUDIDAYA TANAMAN PALAWIJA DENGAN APLIKASI TEKNOLOGI RIZOBAKTERI INDIGENOS DI NAGARI SUNGAI DURIAN KABUPATEN SOLOK Yulmira Yanti; Munzir Busniah; Trimurti Habazar; Zulfadli Syarief; Intan Sari Pasaribu
LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 1 No 2 (2017)
Publisher : Department of Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Andalas Kampus Limau Manis - Padang, Sumatera Barat Indonesia-25163

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2182.949 KB) | DOI: 10.25077/logista.1.2.88-94.2017

Abstract

ABSTRAK Nagari Sungai Durian Kabupaten Solok merupakan salah satu nagari yang memiliki berbagai permasalahan seperti kekeringan, tingginya jumlah lahan tidur dan jauhnya akses. Tanaman utama yang ditanam saat musim hujan adalah padi sawah, namun sebagian besar lahan menjadi lahan tidur saat musim kemarau. Solusi yang dapat ditawarkan adalah dengan penanaman tanaman palawija yang lebih tahan terhadap kekeringan dan penggunaan rizobakteri sebagai agens pengendali hama dan penyakit. Selain sebagai agens pengendali hama dan penyakit, penggunaan rizobakteri juga sebagai PGPR (Plant Growth Promoting Rhizobacteria) yang mampu meningkatkan pertumbuhan dan hasil tanaman. Tujuan dari program pengabdian masyarakat ini adalah memberikan pengetahuan kepada masyarakat dalam budidaya tanaman palawija dengan aplikasi teknologi rizobakteri indigenos. Kegiatan ini dilakukan dengan cara sosialisasi dan demonstrasi plot. Adapun luaran yang didapat dari program pengabdian masyarakat ini adalah :1) Masyarakat mendapatkan pengetahuan serta menguasai teknik budidaya tanaman palawija yang baik (jagung, singkong dan ubi jalar); 2) pengetahuan mengenai teknologi pemanfaatan rizobakteri sebagai agens hayati dalam pengendalian hama dan penyakit tanaman serta peningkatan pertumbuhan dan hasil tanaman palawija; 3) Mendapatkan produk pertanian organik (jagung, singkong dan ubi jalar) yang bebas dari penggunaan pupuk dan pestisida sintetik. Kata kunci : Agens hayati, Rizobakteri, PGPR, Tanaman palawija ABSTRACT Nagari Sungai Durian of Solok District is one of the village that has various problems such as drought, high number of unused land and the distance of urban access. The main crops in this village grown during the rainy season are wetland paddy, but most of the land becomes unused during the dry season. The solution that can be offered is by planting crops that are more resistant to drought and the use of rhizobacteria as pest and disease control agents. In addition to pest and disease control agents, the use of rhizobacteria which also called PGPR (Plant Growth Promoting Rhizobacteria) that can increase growth and yield of plants. The purpose of this community service program is to provide knowledge to the community in the cultivation of secondary crops with the application of indigenous rhizobacteria technology. This activity is done by socialization and demonstration plot. The outcomes obtained from this community service program are: 1) Communities gain knowledge and master good cultivation of secondary crops (corn, cassava and sweet potatoes); 2) knowledge of rhizobacteria utilization technology as biological agent in plant pests and diseases control and improvement of crops' growth and yield; 3) Obtain organic agricultural products (corn, cassava and sweet potato) that are free from the use of synthetic fertilizers and pesticides. Keywords: Biological agents, Rhizobacteria, PGPR, Secondary crops
PENGUATAN KELEMBAGAAN PETANI BARAMBAN DALAM PEMASARAN SAYUR SEHAT BERSERTIFIKAT PRIMA 3 DI KECAMATAN BANUHAMPU KABUPATEN AGAM, SUMATERA BARAT Melinda Noer; Herviyanti Herviyanti; Trimurti Habazar; Cesar Welya Refdi
Jurnal Hilirisasi IPTEKS Vol 4 No 2 (2021)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v4i2.497

Abstract

The Baramban Farmers Group, since 2014, has been carrying out healthy vegetable farming with vegetable products that have been certified Prima 3. However, until now, the management of healthy vegetable farming by the Baramban Farmers Group in Banuhampu District, Agam Regency has not experienced much development in farming because it has not created a broad market with prices that favor the welfare of farmers. Strengthening farmer institutions in marketing healthy vegetables is thus very important and have become an essential element in developing healthy vegetable agribusiness in local, national, and even global markets. The method used is counseling, coaching and mentoring. This service activity is planned to be carried out over the next five years, starting with building a cooperation agreement with the Baramban farmer group starting in 2020. Several alternative solutions to solve the problems of healthy vegetable farmer group partners proposed and agreed upon by the Baramban Farmer Group are: a. Carry out mentoring activities for healthy vegetable farmers by transferring resources from universities through integrated science an agribusiness perspective, b, empowering farmers based on group institutional strengthening to build collective action that is independent of farmers to drive healthy vegetable markets, c. Facilitate farmers to create healthy vegetable market segments through modern markets and online markets with information technology 4.0 and 4. Building partnerships with Transmart as a modern market on a national scale and has a global-scale marketing network. The conclusion is that farmer groups are willing to become partners of the Community Service Team in a community partnership program scheme to help businesses develop.