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All Journal International Journal of Biosciences and Biotechnology Agrikultura AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrosains: Jurnal Penelitian Agronomi PRIMA: Journal of Community Empowering and Services Journal of Biodiversity and Biotechnology Biodiversitas Journal of Biological Diversity Nusantara Bioscience
. Hadiwiyono
Department Of Agriculture Science, Graduated School Of Sebelas Maret University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Other

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Potential of Trichocompost to Improve Organic Garlic Production in Fusarium Wilt–Endemic Fields in Tawangmangu, Central Java Susilo Hambeg Poromarto; Supyani Supyani; Salim Widono; Dwiwiyati Nurul Septariani; Hadiwiyono Hadiwiyono; Argha Hyta Dimas Enggartiasto
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.111410

Abstract

Garlic is a high-value commodity in Tawangmangu, one of its major production centers in Central Java. However, productivity in this region is seriously affected by Fusarium, the pathogen causes basal rot and wilting in the upper parts of the plant. The disease known as basal rot or Fusarium wilt, with the disease intensity in the field can exceeding  60%. Currently, chemical control remains the primary management strategies, which in organic farming, this practice is not acceptable. Therefore, environmentally friendly alternatives are required, such as using organic fertilizers, biological control agent like Trichoderma or combination. Trichoderma is also known as a cellulolytic microbe, which can enhance the composting process and produce Trichompost with improved quality. Field experiment results indicate that Trichocompost (TC) performs better compared to compost (C), Trichoderma (T), or untreated treatment. This findings suggest that garlic treated with Trichocompost and organic fertilizer show better growth with lower wilting intensity. Therefore, there is an indication that increased plant growth can reduce the intensity of Fusarium wilt in garlic.
Pulsed Field Gel Electrophoresis (PFGE): a DNA finger printing technique to study the genetic diversity of blood disease bacterium of banana HADIWIYONO HADIWIYONO; JAKA WIDADA; SITI SUBANDIYAH; MARK FEGAN
Biodiversitas Journal of Biological Diversity Vol. 12 No. 1 (2011)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d120103

Abstract

Hadiwiyono, Widada J, Subandiyah S, Fegan F (2011). Pulsed Field Gel Electrophoresis (PFGE): a DNA finger printing technique to study the genetic diversity of blood disease bacterium of banana. iodiversitas 12: 12-16. Blood disease bacterium (BDB) is the most important pathogen of bananas in Indonesia. In some field, the disease incidence reaches over 80%. Epidemiologically, the disease is similar to Moko disease in South America and Bugtok disease in the Philippines caused by Ralstonia solanacearum race 2. However, BDB is different in phenotype and genotype from the two diseases. Previously BDB was limited in South Sulawesi since 1920s – 1980s and recently was reported in 27 of 30 provinces in Indonesia. Pulsed-Field Gel Electrophoresis (PEGE) is a genomic DNA fingerprinting method, which employs rare cutting restriction endonucleases to digest genome prior to electrophoresis using specialized condition to separate of large DNA fragments. The results showed that PFGE analysis was a discriminative tool to study the genetic diversity of BDB. Based on the PFGE analysis, BDB isolates obtained from different localities in Yogyakarta and Central Java were quit diverse.
Induced resistance by Bacillus subtilis on oil palm seedling infected by Ganoderma boninense FIFI PUSPITA; HADIWIYONO; SUSILO HAMBEG POROMORTO; DEWI INDRIYANI ROSLIM
Biodiversitas Journal of Biological Diversity Vol. 21 No. 1 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210105

Abstract

Abstract. Puspita F, Hadiwiyono, Poromorto S. H, Roslim D. I. 2020. Induced resistance by Bacillus subtilis on Ganoderma boninense infected oil palm seedling. Biodiversitas 21: 28-33. Basal Stem Rot (BSR) caused by Ganoderma boninense Pat is the most important disease in oil palm plantations. The use of biological resistance inducer is a promising alternative disease-control. The research evaluated the potential and mechanism of endophytic Bacillus subtilis as resistance inducer agent of oil palm seedlings to infection of G. boninense. The Experiment was carried out using a completely randomized design consisting of 4 treatments and 3 replications namely of endophytic B. subtilis with four inoculum densities (0, 1011, 1012, and 1013 cfu.mL-1) on oil palm before inoculation of G. boninense. The observation variables were incubation period, disease intensity, growth and concentration of salicylic acid in leaf extract of oil palm seedlings. In addition, the ability of B. subtilis to produce IAA in liquid Pikovskaya medium enriched by tryptophan was assayed. The results showed that B. subtilis was potential as biological resistance inducer agents of oil palm seedlings to infection of G. boninense. The induced resistance was related to increasing growth, salicylic acid in leaf the seedlings and the capability was assayed to produce IAA.
Short Communication: Biological control of Fusarium wilt on banana plants using biofertilizers Arief Widyantoro; HADIWIYONO; SUBAGIYA
Biodiversitas Journal of Biological Diversity Vol. 21 No. 5 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210540

Abstract

Abstract. Widyantoro A, Hadiwiyono, Subagiya. 2020. Biological control of Fusarium wilt on banana plants using biofertilizers. Biodiversitas 21: 2119-2123. Fusarium wilt is an important disease of banana caused by Fusarium oxysporum f. sp. cubense (FOC). The FOC is a weak parasite that attacks many bananas whose conditions are weak such as nutrients. Therefore, controlling Fusarium wilt through fertilization is important so that bananas are not nutrient deficient which can cause plants to be susceptible to FOC. The research aimed to study the effect of biofertilizer applications on FOC suppression in planta. Seven treatments were tested on banana seedlings cv. Ambon Kuning under a completely randomized design: (i) no biofertilizer, (ii) carrier material of biofertilizer, (iii) comparative biofertilizer product, (iv) Azotobacter, (v) Azospirillum, (vi) Streptomyces and (vii) Bacillus. The present study showed that biofertilizer agents were antagonistic to Fusarium wilt. The results showed that biofertilizer agents had the potential to suppress the Fusarium wilt in planta. Streptomyces and Bacillus were the most effective in controlling the Fusarium wilt. Azotobacter and Azospirillum had not been able to prevent the incidence of wilt disease.
Characterization and biocontrol of Pantoea ananatis, the causal agent of rice bacterial leaf blight in Malang, Indonesia LUTFI TRI ANDRIANI; SUSILO HAMBEG POROMARTO; SUPYANI SUPYANI; EDI PURWANTO; HADIWIYONO HADIWIYONO
Biodiversitas Journal of Biological Diversity Vol. 26 No. 4 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260405

Abstract

Abstract. Andriani LT, Poromarto SH, Supyani, Purwanto E, Hadiwiyono. 2025. Characterization and biocontrol of Pantoea ananatis, the causal agent of rice bacterial leaf blight in Malang, Indonesia. Biodiversitas 26: 1537-1544. Disease outbreaks pose a significant obstacle to sustainable agricultural production. Two major diseases affecting rice crops are bacterial leaf blight and blast disease. Bacterial leaf blight caused by Pantoea ananatis is considered new, based on field observations, the cause of bacterial leaf blight is still Xanthomonas oryzae pv. oryzae. Research on P. ananatis is important to provide new knowledge about the causes of bacterial leaf blight other than X. oryzae pv. oryzae. The symptoms of bacterial leaf blight include yellowing and browning of the leaf, followed by drying, resulting in death of the leaf cells. The objectives of this study were to isolate the bacteria, identify them morphologically and molecularly, determine physiological characteristics and explore their biocontrol potential using rhizobacteria. Bacterial characterization was performed both morphologically and physiologically. This study determined the hypersensitive response of tobacco leaves, physiological and biochemical characteristics, pathogenicity tests, and molecular identification via 16S rRNA gene sequencing. The bacteria formed yellow colonies on yeast chalk dextrose agar at 36°C. Isolate was positive for indole and Levan tests, used citrate as a carbon source, and liquefied gelatin at low temperatures. The bacteria showed positive results in tobacco hypersensitivity and pathogenicity tests on paddy rice. Molecular identification results showed that the bacterial pathogen was identified as P. ananatis. The result of biocontrol potential test was shown that bacteria with the code UNS-R2 can control the pathogenic bacteria P. ananatis using spray method. These findings can be used as new knowledge about the types of leaf blight pathogens other than X. oryzae and their control alternatives to determine tactical steps in integrated crop management toward sustainable agriculture. This is the first report on the identification and characterization of P. ananatis in rice in Indonesia and the biocontrol potential using rhizobacteria.
Blood bacterial wilt disease of banana: the distribution of pathogen in infected plant, symptoms, and potentiality of diseased tissues as source of infective inoculums HADIWIYONO HADIWIYONO
Nusantara Bioscience Vol. 3 No. 3 (2011)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n030307

Abstract

Abstract. Hadiwiyono. 2011. Blood bacterial wilt disease of banana: the distribution of pathogen in infected plant, symptoms, and potentiality of diseased tissues as source of infective inoculums. Nusantara Bioscience 3: 112-117. Bacterial wilt caused by blood disease bacterium (BDB) is the most important disease of banana in Indonesia. The disease was difficult to control due to by poorly understood of ecology and epidemiology of the disease. This paper reports the distribution of pathogen-infected plant, symptoms, and potentiality of diseased tissues as source of inoculums. For studying the distribution of BDB in diseased banana, a number of 14 points of plant organ tissue was sampled and the pathogen was detected by PCR using a couple of specific primer for BDB, 121F, and 121R. In addition to the detection of BDB using PCR, both external and internal symptoms were observed. All the points of detection were also used as source of inoculums to search the potentiality of the tissues as source of infective inoculums. The results showed that BDB was distributed systemically in infected banana. The pathogen infection caused systemic symptom and all part of infected banana were potential as source of infective inoculums.
Co-Authors . Sholahuddin Agus Siswanto Andhika Wahyu Nugroho Argha Hyta Dimas Enggartiasto Arief Widyantoro Arief Widyantoro Bayu Rahmad Bernadip Budi Tjahjono Claudia Sandy Sofani Dedy Prasetyo Dedy Prasetyo Devi Puji Rahayu Devi Puji Rahayu DEWI INDRIYANI ROSLIM Dewi Indriyani Roslim Dwiwiyati Nurul Septariani Dwiwiyati Nurul Septariani Dwiwiyati Nurul Septariani, Dwiwiyati Nurul EDI PURWANTO Endang Sulastri Fifi Puspita FIFI PUSPITA Fitriana, Faedah Haqiki, Zahra Tazkiyatun Hardjono Sri Gutomo, Hardjono Sri I Gusti Bagus Wiksuana I Ketut Suada JAKA WIDADA Kumala Sari Lulu'il Maknun LUTFI TRI ANDRIANI MARK FEGAN Mawar, Munti'ah Mohammad Fikri Halim Madjid Natya Lakshita Poromarto, Susilo Hambeg Puji Harsono, Puji Salim Widono Salim Widono SITI SUBANDIYAH Subagiya Subagiya Sudadi Sudadi Sudadi Sudadi Sudadi Sudadi SUPYANI SUPYANI Supyani Supyani Supyani Supyani Supyani Supyani SUSILO HAMBEG POROMARTO SUSILO HAMBEG POROMORTO Susilo Hambeg Poromarto Susilo Hambeg Poromarto Susilo Hambeg Poromarto Susilo Hamberg Poromarto Vita Ratri Cahyani Vita Ratri Cahyani Zahra Tazkiyatun Haqiki