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All Journal Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research
Hamny Hamny
Fakultas Kedokteran Hewan, Universitas Syiah Kuala
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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STUDI ANATOMIS DAN HISTOLOGIS PANKREAS BIAWAK AIR (Varanus salvator) (Anatomical and Histological Study of the Pancreas of Water Monitor Lizard (Varanus salvator)) Hamny, Hamny; Iqbal, Muhammad; Wahyuni, Sri; Sabri, Mustafa; Jalaluddin, M.; Rinidar, Rinidar
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5047

Abstract

The aim of this research was to determine the anatomy and histology of pancreas of water monitor lizard (Varanus salvator). Two pancreas were used in this research (a male and a female). Water monitor lizard was euthanized using chloroform and subsequently necropsied to collect the pancreas. The pancreas was washed in 0.9 % NaCl solution. Anatomical observations were carried out including location, shape, and color of pancreas, also the length and weight measurements. Then, pancreas was fixed with 4% paraformaldehyde for 7 days to proceed to histological preparation and hematoxylin eosin (HE) staining. The results of the anatomical observations showed that pancreas of water monitor lizard have two lobes, dorsal lobe (splenic lobe) and ventral lobe (duodenal lobe). Dorsal lobe which was in oval form adhered with the spleen, and ventral lobe was located in the cranial of intestines with the form of long spherical. Both of pancreas lobes were white-yellow color with the length of 1.450.64 cm (dorsal lobe) and 7.000.42 cm (ventral lobe). Weight of dorsal lobe was 0.150.01 g and ventral lobe was 6.352.30 g. The histological observations found the acinar cells, ducts, and blood vessels in both of pancreas lobes. Additionally, the similar structure of Langerhans islet only found at the ventral lobe of pancreas. In conclusion, pancreas of water monitor lizard consists of two lobes which located differently. Ventral lobe is larger than the dorsal lobe. Both lobes have similar histological structure except the Langerhans islets are only appeared in the ventral lobe.
STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)) Wahyuni, Sri; Agungpriyono, Srihadi; Adnyane, I. Ketut Mudite; Hamny, Hamny; Jalaluddin, Muhammad; Gholib, Gholib; Akmal, Muslim; Adam, Mulyadi; Aliza, Dwinna; Siregar, Tongku Nizwan
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3396

Abstract

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 g/ml of concentration for PNA lectins and 15g/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates -galactose, -glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis.Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak
Histology and histomorphometry of the skin in the ventral abdominal region of domestic fowl (Gallus gallus domesticus), duck (Anas platyrhynchos), and doves (Columba livia) Zainuddin, Zainuddin; Rahmi, Erdiansyah; Akmal, Muslim; Masyitha, Dian; Ismail, Ismail; Hamny, Hamny; Pratiwi, Siska; Zulkifli, Baidillah
The International Journal of Tropical Veterinary and Biomedical Research Vol 9, No 1 (2024): Vol. 9 (1) May 2024
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v9i1.40134

Abstract

This study aims to determine the histological and histomorphometric characteristics of the skin of domestic fowl (Gallus gallus domesticus), ducks (Anas platyrhynchos), and doves (Columba livia) in the ventral abdominal region. Each study sample consisted of six individuals. The samples were processed into histological preparations with a thickness of 5 m and then stained using Haematoxylin-Eosin (HE). The results showed that the histological structure of the skin of domestic fowl, ducks, and doves is relatively thin compared to the structure of mammalian skin and consists of the epidermis, dermis, and hypodermis (subcutis). The histomorphometric data analysis showed that the thickness of the epidermal layer of the skin in the ventral abdominal region of domestic fowl, ducks, and doves was not significantly different (P0.05), but there were significant differences (P0.05) in the thickness of the dermis and hypodermis. It can be concluded that a similarity exists in the histological structure of the skin of domestic fowl, ducks, and doves, but differences are observed in the histomorphometric properties of the skin in the ventral abdominal region of these birds.
Co-Authors Dian Masyitha Dwinna Aliza Erdiansyah Rahmi Faisal Mustafa Gholib Gholib, Gholib I. Ketut Mudite Adnyane, I. Ketut Mudite Ismail Ismail M. Jalaluddin, M. Muhammad Iqbal Muhammad Jalaluddin, Muhammad Mulyadi Adam, Mulyadi Muslim Akmal Rinidar Rinidar, Rinidar Siska Pratiwi Sri Wahyuni Srihadi Agungpriyono Tongku Nizwan Siregar Zainuddin, Zainuddin Zulkifli, Baidillah