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All Journal AVERROUS: Jurnal Kedokteran dan Kesehatan Malikussaleh Narra J Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Trends in Infection and Global Health Animal Production
Gholib Gholib, Gholib
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Effect of black garlic (Allium sativum) on gonadosomatic index, follicle-stimulating hormone level and spermatozoa quality: A study in monosodium glutamate-exposed rat model Bani, Ika I.; Zulkarnain, Zulkarnain; Gholib, Gholib; Syahrizal, Dedy; Nugraha, Taufiq P.; Ramadhan, Akmal; Akmal, Muslim; Maulina, Nora
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.617

Abstract

Infertility rates have risen significantly, one of which is due to monosodium glutamate (MSG) consumption. Recent studies have shown that flavonoids in black garlic (Allium sativum) act as antioxidants. The aim of this study was to assess the effect of black garlic extract (BGE) on gonadosomatic index, follicle-stimulating hormone (FSH) levels, and spermatozoa quality in rats exposed to MSG. Twenty-five healthy rats, aged ten to twelve weeks, were divided equally into five experimental groups: (1) negative control (NC), no intervention; (2) positive control (PC), fed with MSG 8 mg/kg; and (3) fed with MSG + BGE 200 mg/kg; (4) fed with MSG + BGE 400 mg/kg; and (5) fed with MSG + BGE 600 mg/kg. Oral MSG was administered once a day for two weeks before BGE administration was started for two weeks. The measured endpoints were gonadosomatic index, FSH levels, and spermatozoa concentration and quality (spermatozoa motility and abnormality). Analysis of variance (ANOVA) followed by Duncan's post hoc analysis was used to assess the measurement differences. The result suggested that the administration of BGE did not significantly affect the gonadosomatic index (p=0.513). Significant decreases in FSH levels (p=0.005) and spermatozoa concentration were observed in the PC group compared to other groups (p<0.001). Additionally, spermatozoa motility was significantly lower in the PC group compared to NC, BGE200, BGE400, and BGE600 (p<0.001), with higher motility noted in BGE200, BGE400, and BGE600 compared to PC (p<0.001). Furthermore, PC had significantly higher spermatozoa abnormalities compared to NC, BGE200, BGE400, and BGE600 (p<0.001). In conclusion, administration of BGE had a significant effect on the improvement of FSH levels and the quality of spermatozoa in rats exposed to MSG.
Black garlic exhibited hepatoprotective effect against monosodium glutamate-induced hepatotoxicity in animal model Maulina, Nora; Zachreini, Indra; Gholib, Gholib; Suwandi, Abdulhadi; Akmal, Muslim
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.799

Abstract

Monosodium glutamate (MSG) is commonly used as a flavor-enhancing agent in foods, and studies have demonstrated its toxic effects in animal models. Black garlic is known for its antioxidant and anti-inflammatory properties; however, there is a lack of studies on the potential hepatoprotective effect of black garlic ethanol extract (BGE) against MSG-induced hepatotoxicity in rats. The aim of this study was to investigate the hepatoprotective effects of ethanol extract of black garlic against MSG-induced liver damage in animal model. Twenty-five male Wistar rats were randomly assigned to five groups (n=5): negative control, MSG only, and MSG with three different doses of BGE. The MSG only and MSG with BGE groups were orally administered with 8 mg/kg MSG daily. After MSG treatment, the MSG with BGE groups received BGE orally at daily doses of 200, 400, or 600 mg/kg body weight for 16 consecutive days. Subsequently, the levels of serum liver enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), interferon-gamma (IFN-γ), and cyclooxygenase-2 (COX-2) were measured. Our data indicated that the group treated with 200 mg/kg BGE had significant lower levels of AST and ALT significantly compared to the MSG-only group. The MSG-treated group had higher levels of the inflammatory markers COX-2 and IFN-γ, which were lowered by administration of 200 mg/kg BGE. In contrast, higher doses of BGE led to greater levels of COX-2 and IFN-γ compared to those in the MSG-only group. This study suggested that BGE might have hepatoprotective effects at low dose, potentially mitigating MSG-induced liver damage. However, the higher dose of black garlic extract did not alleviate inflammation, as shown by the higher levels of COX-2 and IFN-γ.
Potential of ant nest extract (Hydnophytum formicarum) for protection of testicular morphometry, epididymal functions, and sperm quality in male rats with alloxan-induced diabetes Roslizawaty, Roslizawaty; Abrar, Mahdi; Khairan, Khairan; Gholib, Gholib; Siregar, Tongku N.; Syafruddin, Syafruddin; Sutriana, Amalia; Azzahra, Putri; Aini, Hanipah
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.922

Abstract

Medicinal herbs, such as the ant nest plant (Hydnophytum formicarum), are promising for the management of diabetes mellitus-associated infertility. The aim of this study was to evaluate the biological activity of the ant nest plant and its capacity to mitigate the adverse effects of alloxan-induced diabetes on testicular morphology, epididymal function, and sperm quality in male rats. The tuber of the ant nest plant was extracted using methanol and then subjected to phytochemical screenings. For the experiment, 20 male white rats (Rattus norvegicus), aged 3–4 months and weighing 150–200 g, were equally divided into four groups. The ant nest extract was administered orally using oral gavage over 14 days. The testes, epididymis, and sperm were collected for weighing, morphometric measurements, and quality evaluation. Qualitative testing of phytochemical compounds indicated the presence of flavonoids, tannins, steroids, terpenoids, and phenolic compounds in the plants. The results revealed the protective effects of ant nest extract against the adverse fertility effects induced by alloxan and a high-fat diet, as observed in testicular weight (p=0.045), epididymal weight (p=0.041), and sperm quality (p>0.05).
The Quality of Organic Waste Market Ensiled Using Rejected Commercial Syrup as an Alternative Ruminant Livestock Feed Sadarman, Sadarman; Febrina, Dewi; Rinaldi, Satria Trisna; Hendri, Hendri; Ichwan Ilyazar, Mohammad; Weno, Weno; Alfian, Aldi; Amalia Nurfitriani, Rizki; Qomariyah, Novia; Sukmara, Anne; Koswara, Eko; Prihambodo, Tri Rachmanto; Gholib, Gholib; Faiz Mohd Azmi, Amirul
ANIMAL PRODUCTION Vol. 25 No. 3 (2023)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2023.25.3.257

Abstract

Market organic waste (LOP) is organic residue or residue generated from market trading activities such as residual vegetables, fruits, and other organic foodstuffs. This waste can be a source of environmental problems if not managed properly because it can rot and cause unpleasant odors. Still, if managed properly, it can be used as an alternative feed for livestock. This study aims to evaluate the quality of LOP silage using rejected commercial syrup as silage additives. The manufacture, harvesting, drying, and siege of silage are conducted at the Laboratory of Nutrition and Feed Technology, Faculty of Agriculture and Animal Husbandry, Sultan Syarif Kasim State Islamic University, Riau. The experimental method with a Complete Randomized Design consisting of 5 treatments and five repeats was used in this study. The treatment in question was P1: spinach 33.3% + cabbage 33.3% + kale 33.3% as control, for P2, P3, P4, and P5 plus SKA of 1%, 2, 3, and 4%, respectively, based on dry matter, then enzymatic for 30 days at room temperature. The parameters measured are temperature, humidity, mold growth, and silage pH. The data obtained were analyzed based on variety analysis, and the difference in parameter values between treatments was further tested with a DMRT level of 5%. The results of the variety analysis showed that the use of SKA had a significant effect (P<0.05) on temperature, humidity, fungal growth, and silage pH. The silage temperature at the time of harvesting averages 30±0.39°C; the average humidity is 77±0.25%; mushroom growth averaged 2.87±0.39%; and an average silage pH of 3.67±0.45. The conclusion is that SKA can improve the quality of market organic waste silage by optimizing temperature and humidity during ensilage to minimize pH and mold growth.
The number of Leydig cells in highfat diet-fed rats after administration of kepok banana peel extract Zulkifli, Baidillah; Akmal, Muslim; Wahyuni, Sri; Gholib, Gholib; Adam, Mulyadi; Zainuddin, Zainuddin; Lubis, Triva Murtina; Usrina, Nora; Nirmala, Fadya
The International Journal of Tropical Veterinary and Biomedical Research Vol 7, No 2 (2022): Vol. 7 (2) November 2022
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v7i2.30689

Abstract

The impact of free radicals on testicular Leydig cells can result in the disruption of male reproductive health. The present study aimed to evaluate the effects of a high-fat diet on Leydig cells and the potential of kepok banana peel extract as a medication to mitigate these effects. In total of 20 adult male Wistar rats were assigned to five groups and treated with standard feed (P1), high-fat feed (P2), high-fat feed with simvastatin (P3), and high-fat feed with banana peel extract at doses of 100 and 200 mg/kg BW (P4 and P5). After 60 days of treatment, the number of Leydig cells was determined using HE staining methods. Data were analyzed using an One Way ANOVA test. The results showed that the administration of 100 mg/kg BW of kepok banana peel extract (P4) was able to maintain the number of Leydig cells and counteract the negative effects of a high-fat diet. These findings suggest that kepok banana peel extract may have the potential as a herbal medicine for supporting male reproductive health.
Effects of Black Garlic Extract Against Monosodium Glutamate-Induced Liver Damage in Rats: Macroscopic Studies Maulina, Nora; Zachreini, Indra; Gholib, Gholib; Akmal, Muslim
AVERROUS: Jurnal Kedokteran dan Kesehatan Malikussaleh Averrous, Volume 11 No.1 Mei 2025
Publisher : Fakultas Kedokteran Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/averrous.v11i1.18815

Abstract

Monosodium glutamate (MSG) enhances flavor during food processing by providing an umami taste, but its metabolic by-products can cause liver injury. The liver is crucial for the metabolism of xenobiotics including glutamate. Black garlic, derived from fresh garlic (Allium sativum L.) through chemical reactions and heat treatment, may have hepatoprotective properties; however, studies are limited. This study assessed the hepatoprotective effects of black garlic extract (BGE) on MSG-induced liver damage. Twenty-five Wistar rats (Rattus norvegicus) were divided into five groups (n = 5): P0 (negative control); P1 (MSG group), 8 mg/kg MSG for 21 days; and P2, P3, and P4, receiving the same MSG protocol as P1 plus BGE doses of 200, 400, and 600 mg/kg body weight, respectively, for 16 days. After the experiment, rats were euthanized for liver tissue extraction. Although liver weights in the BGE-treated groups were lower than those in the MSG group, the difference was not statistically significant. These results indicated that a BGE dose of 200 mg/kg body weight mitigated MSG-induced hepatic damage. Further biochemical and histological studies are required to elucidate the mechanism of BGE's hepatoprotective action of BGE against MSG-induced hepatotoxicity.
EFFECT OF ETHANOLIC NEEM (Azadirachta indica) LEAVES EXTRACT ON DEVELOPMENT OF SPERMATID AND ANDROGEN RECEPTOR EXPRESSION IN THE TESTIS OF RABBIT Aulia, Usma; Wahyuni, Sri; Gholib, Gholib; Dasrul, Dasrul; Adam, Mulyadi; Rahmi, Erdiansyah; Hamzah, Abdullah
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i1.30042

Abstract

The objective of this study was to determine the effect of ethanol extract of neem (Azadirachta indica) leaves on the number of spermatids and androgen receptor expression in the testicular tissue of New Zealand White (NZW) rabbits. Twelve adults male NZW rabbits with body weight (BW) 2-2.5 kg were divided into three treatment groups (n = 4), namely P1 (control group) given 0 mg/kg BW, P2 and P3 were given 200 mg/kg BW and 300 mg/kg BW of neem leaves ethanol extract respectively. The extract was administered orally using a gastric tube for 52 days. At the end of the treatment, the rabbits were terminated and the testes were collected to be processed into histological preparations and stained with Periodic Acid Schiff (PAS) staining to detect the number of spermatid and Immunohistochemical (IHC) staining to detect androgen receptor expression. The results showed that neem leaves extract administered at P3 rabbits significantly decreased the number of spermatids and androgen receptor expression in myoid peritubular cells and connective tissue P0.05), but not significantly different in the Leydig cells and blood vessels (P0.05) of testicular tissue. The conclusion of this study is that the ethanol extract of neem leaves at a dose of 300 mg/kg BW can be used as a contraceptive candidate compared to a dose of 200 mg/kg BW.
CHARACTERIZATION OF GLYCOCUNJUGATE DISTRIBUTION IN THE EPIDIDYMIS OF THE JAVAN MUNTJACS (Muntiacus muntjak muntjak) Wahyuni, Sri; Sofyan, Hamny; Hafizuddin, Hafizuddin; Adnyane, I Ketut Mudite; Gholib, Gholib; Jalaluddin, Muhammad; Gani, Fadli A.; Adam, Mulyadi; Siregar, Tongku Nizwan; Agungpriyono, Srihadi
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.24418

Abstract

The presence of glycoconjugates in caput, corpus, and cauda epididymis of the Javan muntjacs was performed using lectin histochemical method. The epididymis organs were obtained from two adult male Javan muntjacs in hard antler stage and then immediately fixed in Bouins solution and processed in histological manner. Six types of biotinylated lectins namely Peanut agglutinin (PNA), Soybean agglutinin (SBA), Wheat germ agglutinin (WGA), Ricinus communis agglutinin (RCA), Concanavalin A (Con A), and Ulex europaeus agglutinin I (UEA I) were applied to detect the specific glycoconjugates in the epididymal tissue of muntjacs. The result showed the presence of glycoconjugates were detected by five types of lectins except UEA I in caput, corpus, and cauda epididymis with specific distribution and also the intensity of lectins binding. Glycoconjugates -galactose, -glucose, mannose, N-acetyl-galactosamine, N-acetyl-glucosamine, and sialic acid were stained with various intensity in epithelial cells, basal cells, stereocilia, spermatozoa, and stroma of caput, corpus, and cauda epididymis. The presence of sialic acid on the surface of spermatozoa detected by WGA are thought to be play a role on sperm protection during their maturation and transit along epididymal lumen. In conclusion, the difference of glycoconjugates distribution pattern and lectin binding in the epididymal caput, corpus, and cauda of the Javan muntjac indicates the regional differences in epididymal function in spermatozoa maturation.
CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE Adam, Mulyadi; Gholib, Gholib; Hafizuddin, Hafizuddin; Zamzami, Rumi Sahara; Bahi, Muhammad
Jurnal Kedokteran Hewan Vol 13, No 4 (2019): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i4.12890

Abstract

The objective of this study was to characterize the morphology of ovary from slaughtered Aceh cow and measuring the concentration of estradiol in the follicular fluid to examine the potential utilizing for in-vitro fertilization (IVF) development in Aceh cattle. In total, 30 pairs of ovaries were collected from 30 Aceh cattle slaughtered at Banda Aceh abattoir. Morphological characteristics of ovaries observed were the size of ovaries, weight of ovaries, number of follicles, the diameter of follicles, corpus luteal (CL), and volume of follicular fluid. The follicular fluid was aspirated using a disposable syringe and analyzed the estradiol concentrations using enzyme-linked immunosorbent assay (ELISA). The follicles were grouped into 3 categories based on the diameter () of follicles: small follicles ( 1 - 5 mm), medium follicles ( 5 - 8 mm), and large follicles ( 8 mm). The morphological characteristic of ovaries varied between right and left ovaries in which right ovaries showed bigger size and weight compared to the left ovaries (P0.05), whereas the number of follicles and follicular fluid did not significantly different (P0.05). The concentrations of estradiol in follicular fluid of large and medium follicles were significantly higher compared to small follicles (P0.05), whereas the concentration of estradiol between large and medium follicles, did not significant different (P0.05). In conclusion, the follicles from abattoir can be used as potential oocytes resources to support IVF development for Aceh cattle in the future. The follicular fluid provided from these follicles can be an alternative for in vitro maturation media of oocytes due to containing high estradiol concentrations.
STEROID LEVEL AND PREGNANCY RATE OF ACEH COWS IN RESPONSE TO OVULATION INDUCTION USING PRESYNCHOVSYNCH METHOD Adam, Mulyadi; Siregar, Tongku Nizwan; Wahyuni, Sri; Gholib, Gholib; Ramadhana, Cut Erika; Ananda, Riski; Afifuddin, Afifuddin
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.8994

Abstract

Penelitian ini bertujuan mengetahui peningkatan level steroid dan persentase kebuntingan sapi aceh terhadap induksi ovulasi dengan metode presynch-ovsynch. Dalam penelitian ini digunakan sepuluh ekor sapi aceh betina dengan status tidak bunting, minimal dua bulan pascapartus, sudah pernah beranak, dan sehat secara klinis. Sapi dibagi atas dua kelompok, yang masing-masing terdiri atas lima ekor sapi. Kelompok pertama (K1) disinkronisasi berahi dengan metode presynch-ovsynch. Pada kelompok kedua (K2), disinkronisasi berahi menggunakan 5 ml PGF2 secara intramuskulus dengan pola penyuntikan ganda dengan interval 12 hari. Setelah 48 jam akhir perlakuan, sapi pada K1 dan K2 diinseminasi menggunakan semen beku fertil. Observasi berahi dilakukan setelah penyuntikan terakhir. Koleksi darah untuk pemeriksaan level estradiol dilakukan segera setelah inseminasi dilakukan sedangkan koleksi darah untuk pemeriksaan progesteron dilakukan pada hari ke-7 pasca-inseminasi. Level steroid diukur menggunakan teknik enzyme-linked immunosorbent assay (ELISA). Pemeriksaan kebuntingan dilakukan 90 hari pasca-inseminasi menggunakan teknik palpasi rektal. Seluruh sapi menunjukkan gejala berahi setelah perlakuan. Level estradiol dan progesteron pada K1 vs K2 masing-masing adalah 294,98110,48 vs 392,7611,6 pg/ml (P0,05) dan 23,8515,14vs 12,695,64ng/ml (P0,05). Persentase kebuntingan pada K1 vs K2 masing-masing adalah 60,0 vs 0,0%. Dari hasil penelitian disimpulkan bahwa metode presynch-ovsynch tidak dapat meningkatkan level steroid tetapi dapat meningkatkan persentase kebuntingan pada sapi aceh.
Co-Authors Abdullah Hamzah, Abdullah Afifuddin Afifuddin, Afifuddin Aini, Hanipah Alfian, Aldi Amalia Sutriana Antje Engelhardt, Antje Aulia, Usma Azzahra, Putri Bambang Purwantara Bani, Ika I. Dasrul Dasrul Dedy Syahrizal Dewi Febrina Dwinna Aliza Erdiansyah Rahmi Faisal Mustafa Faiz Mohd Azmi, Amirul Fajar Fakri Fajri, Fauzan Gani, Fadli A. Hafizuddin Hafizuddin Hamny Hamny Hamny Sofyan Hanifah Yusuf Heistermann, Michael Hendri Hendri I. Ketut Mudite Adnyane, I. Ketut Mudite Ichwan Ilyazar, Mohammad Iman Supriatna Ketut Adnyane Mudite Khairan Khairan Koswara, Eko Mahdi Abrar Masda Admi MUHAMMAD AGIL Muhammad Bahi Muhammad Jalaluddin, Muhammad Mulyadi Adam, Mulyadi Muslim Akmal Nanda Yulian Syah Nirmala, Fadya Nora Maulina, Nora Nora Usrina Putri Handayani Qomariyah, Novia Ramadhan, Akmal Ramadhana, Cut Erika Rinaldi, Satria Trisna Riski Ananda, Riski Rizki Amalia Nurfitriani, Rizki Roslizawaty Roslizawaty, Roslizawaty Sadarman , Siregar, Tongku N. Sri Wahyuni Srihadi Agungpriyono Sukmara, Anne Suwandi, Abdulhadi Syafruddin Syafruddin Taufiq P. Nugraha, Taufiq P. Tongku Nizwan Siregar Tri Rachmanto Prihambodo Triva Murtina Lubis Weno, Weno Yusuf, Hanifa Zachreini, Indra Zainuddin, Zainuddin Zamzami, Rumi Sahara Zulfitri Zulfitri, Zulfitri Zulkarnain Zulkarnain Zulkifli, Baidillah