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All Journal Microbiology Indonesia Jurnal Ilmiah Mahasiswa Kelautan Perikanan Unsyiah Aceh International Journal of Science and Technology Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Sofyatuddin Karina
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Immunology & microbiology Medicine & Pharmacology

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Pemanfaatan ekstrak daun inai (Lawsonia inermis l.) sebagai bakterisida terhadap Aeromonas hydrophila yang menginfeksi ikan lele sangkuriang (Clarias gariepinus) Sofyatuddin Karina; Mimie Saputri; Muhammad Naufal
Depik Vol 4, No 3 (2015): DECEMBER 2015
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.4.3.3056

Abstract

Abstract. The objective of this research was to find out the effectiveness of henna leaf extract (Lawsonia inermis L.) on A. hydrophila in vitro and its effect on the survival rate of Clarias gariepinus that infected by those bacterias in vivo. The research was conducted at chemistry laboratory of Teacher Training and Education Faculty, Syiah Kuala University and at Microbiology laboratory, Brackishwater aquaculture Department Center, Ujong Batee on Avril to September, 2015. The research involved in vitro test with the inhibition zone as measured parameter and in vivo test with the survival rate value as measured parameter. The treated concentrations were 1, 2, 3, 4 and 5% of extract. The result of in vitro test showed that henna leaf extract was effective against A. hydrophila with the inhibition zone for 1, 2, 3, 4, and 5% extract were 5.45; 5.7; 6.5; 7.5; and 9.6 mm, respectively. The result of ANOVA test showed that henna leaf extract has a significant effect on the survival rate of catfish (P0.05). The best survival rate was obtained at 4% of extract  concentration.  Keywords: Aeromonas hydrophila; henna leaf extract; catfishAbstrak. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak daun inai (Lawsonia inermis L.) terhadap A. hydrophila secara in vitro dan pengaruhnya secara in vivo terhadap kelangsungan hidup ikan lele sangkuriang (Clarias gariepinus) yang terinfeksi bakteri ini dengan cara perendaman. Penelitian ini dilakukan di Laboratorium kimia, FKIP Unsyiah dan Laboratorium Mikrobiologi Balai Budidaya Perikanan Air Payau (BBAP) Ujong Batee pada dari bulan April hingga bulan September 2015. Penelitian ini meliputi uji in vitro dengan parameter uji berupa zona hambat ekstrak terhadap bakteri dan uji in vivo dengan parameter uji berupa nilai kelangsungan hidup ikan lele sangkuriang pasca perlakuan dengan ekstrak. Konsentrasi perlakuan yang dilakukan adalah 1, 2, 3, 4, dan 5%. Hasil uji in vitro menunjukkan bahwa ekstrak daun inai (Lawsonia inermis L.) terbukti efektif terhadap Aeromonas hydrophila dengan nilai zona  hambat secara berturut-turut terhadap konsentrasi 1, 2, 3, 4 dan 5% adalah 5,45; 5,7; 6,5; 7,5; dan 9,6 mm. Hasil uji ANOVA menunjukkan bahwa ekstrak daun inai berpengaruh nyata (P0,05) terhadap kelangsungan hidup ikan lele sangkuriang. Tingkat kelangsungan hidup ikan terbaik diperoleh pada konsentrasi ekstrak 4%.Kata kunci : Aeromonas hydrophila; ekstrak daun inai; ikan lele sangkuriang   
Co-Authors AA Sudharmawan, AA Ade Siswanto Aris Munandar Chitra Octavina Cut Yulvizar Desi Warni Dian Rahmi Dwinna Aliza Eggy Havid Parmadi FARAH SYAHLIZA Gian Rieza Pratama Hayatun Nufus Ichsan Setiawan Iko I. Arisa Ilham Gianda Tilas Imelda Astuti Irma Dewiyanti Jamnur Syahputra Januar Nanda Khairunnisa Khairunnisa Kinang Kinang Latifah Rizkiana Lindawaty Lindawaty M. Sarong Ali Mimie Saputri Muhammad Naufal Muhammad R. Maulana Muhammad Rizki Ramadhan Muhammad Syukur Muhibuddin Muhibuddin Musri Musman Nofra Rizqiyah Novita Novita Nurfadillah Nurfadillah Panji Muhammad Maulana Putri Aprilia Rahmatullah Rahmatullah Ramelan Ramelan Rifki Rahman Riski Muliawan Riza Andrila Rizka V Ayudia Rizky Rajuliansyah Lubis Rony, Zahara Tussoleha Ryansyah Putra Affandi Saniah . Sasnita Sasnita Sayyid Afdhal El-Rahimi Siska Mellisa SUCI FARADILLA Sugeng, Santoso Syahrul Purnawan Syahrul Purnawan Tarmizi Tarmizi TATI NURHAYATI Teuku Reza Ferasyi Umi Karmila Viqqi Kurnianda Yopi Ilhamsyah