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Journal : Jurnal Natural

IMMUNOSTIMULATORY EFFECT OF METHANOL EXTRACT OF FLAMBOYANT LEAF [Delonix regia (Boj. ex Hook.) Raf.] IN MICE Kartini Eriani; Ainsyah Ainsyah; Rosnizar Rosnizar; Yunita Yunita; Ichsan Ichsan; Al Azhar
Jurnal Natural Volume 18, Number 1, February 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (513.493 KB) | DOI: 10.24815/jn.v18i1.9830

Abstract

Abstract. Flamboyant [Delonix regia (Boj. ex Hook) Raf.] leaf contains flavonoid compounds that are expected to have immunostimulatory effect. This research was done to determine the effect of flamboyant leaf extract on immune response by accessing the activity of immune cells and capability test the extract as immunostimulant in mice. Leaf extraction was done by maceration using methanol in the Laboratory of Biology of Chemistry Department, Faculty of Mathematics and Natural Sciences of Syiah Kuala University whereas animal treatment and testing were carried out Micro-technique Laboratory of Biology Department of the same faculty. This research used 20 male mice strain Swiss-Webster aged 7-8 weeks were randomly assigned to 4 treatment groups with five replications each. Group 1 (P0) was untreated control; group 1-3 were mice administration flamboyant leaf extract 250 mg/kg BW (P1), 500 mg/kg BW (P2), and 750 mg/kg BW (P3) per oral. The treatments were given for 14 days after one week of adaptation period. Blood samples were collected before and after extract treatment and used for leukocyte count analysis. Phagocytosis activity was accessed by carbon clearance assay on day 15. At the end of the study, all mice were sacrificed for spleen weight analysis. Data obtained was analyzed by Analysis of Variance followed by Tukey test (Leukocyte count and spleen weight) or regression analysis (carbon clearance). The results showed a flamboyant leaf extract administration resulted in increased leukocyte counts that were significantly different (p0.05) between treatment groups.  Phagocytosis test indicated the extract had moderate to strong immunostimulatory effect whereas spleen weight analysis did not show any difference among treatment groups. In conclusion, flamboyant leaf methanol extract was able to increase immune cells and had potential immunostimulatory activity in mice.Keywords: Delonix regia, immunostimulant, leukocytes, lymphocyte proliferation.
IMMUNOSTIMULATORY EFFECT OF METHANOL EXTRACT OF FLAMBOYANT LEAF [Delonix regia (Boj. ex Hook.) Raf.] IN MICE Kartini Eriani; Ainsyah Ainsyah; Rosnizar Rosnizar; Yunita Yunita; Ichsan Ichsan; Al Azhar
Jurnal Natural Volume 18, Number 1, February 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v18i1.9830

Abstract

Abstract. Flamboyant [Delonix regia (Boj. ex Hook) Raf.] leaf contains flavonoid compounds that are expected to have immunostimulatory effect. This research was done to determine the effect of flamboyant leaf extract on immune response by accessing the activity of immune cells and capability test the extract as immunostimulant in mice. Leaf extraction was done by maceration using methanol in the Laboratory of Biology of Chemistry Department, Faculty of Mathematics and Natural Sciences of Syiah Kuala University whereas animal treatment and testing were carried out Micro-technique Laboratory of Biology Department of the same faculty. This research used 20 male mice strain Swiss-Webster aged 7-8 weeks were randomly assigned to 4 treatment groups with five replications each. Group 1 (P0) was untreated control; group 1-3 were mice administration flamboyant leaf extract 250 mg/kg BW (P1), 500 mg/kg BW (P2), and 750 mg/kg BW (P3) per oral. The treatments were given for 14 days after one week of adaptation period. Blood samples were collected before and after extract treatment and used for leukocyte count analysis. Phagocytosis activity was accessed by carbon clearance assay on day 15. At the end of the study, all mice were sacrificed for spleen weight analysis. Data obtained was analyzed by Analysis of Variance followed by Tukey test (Leukocyte count and spleen weight) or regression analysis (carbon clearance). The results showed a flamboyant leaf extract administration resulted in increased leukocyte counts that were significantly different (p0.05) between treatment groups.  Phagocytosis test indicated the extract had moderate to strong immunostimulatory effect whereas spleen weight analysis did not show any difference among treatment groups. In conclusion, flamboyant leaf methanol extract was able to increase immune cells and had potential immunostimulatory activity in mice.Keywords: Delonix regia, immunostimulant, leukocytes, lymphocyte proliferation.
The immunostimulant effect of jamblang stem bark (Syzygium cumini L.) ethanol extract against mice macrophages phagocytosis activity and capacity Rosnizar, Rosnizar; sari, widya; Syahfitri, Widya; Kusuma, Hendrix Indra; safitri, novi; izzazaya, annisa
Jurnal Natural Volume 25 Number 2, June 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to determine the effect of administering Jamblang Stem Bark Ethanol Extract (Syzygium cumini L.) (JSBEE) on the activity and phagocytosis capacity of macrophages in mice (Mus musculus) infected with Staphylococcus aureus bacteria. The research method used a completely randomized design (CRD) with five treatments and five repetitions, conducted in-vitro and in vivo test. The treatments for in vitro involved administering distilled water (T0), Stimuno (T1), and JSBEE at concentrations of 10 ppm (T2), 100 ppm (T3), and 1000 ppm (T4). Subsequently, in vivo treatment was conducted using distilled water (T0), stimuno (T1), JSBEE at 10 mg/kg (T2), 100 mg/kg (T3), and 1000 mg/kg (T4). Initially, mice underwent in vivo administration of JSBEE, administered orally via catheter tip, with a dosage of 1 mL per 10 g of body weight. JSBEE was administered orally for 10 days, followed by infection with S. aureus on the 11th day. The in vitro tests were conducted by isolating macrophage cells from the intraperitoneal fluid, to which S. aureus and JSBEE were added. Intraperitoneal fluid collected from the mice was used to prepare smears using the thin blood smear method and Giemsa staining. Macrophage phagocytosis activity was observed and assessed based on the percentage activity formula, and the phagocytic capacity of macrophages was measured by the number of S. aureus cells phagocytosed. The results indicated that JSBEE significantly affected (P0.05) both the activity and phagocytic capacity of macrophages in vivo and in-vitro. The best concentration of JSBEE for increasing both the activity value and phagocytic capacity of macrophages was 100 ppm (T3).