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Analisis Penambahan Ion Na dan Ca pada Hidrolisis Pati Singkong (Manihot esculanta) untuk Meningkatkan Aktivitas Enzim: Studi Kinetika Hidrolisis Hargono, Hargono; Jos, Bakti; Satriadi, Hantoro; Zakaria, Muhammad Fahmi
TEKNIK Vol. 43, No. 1 (2022): May 2022
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/teknik.v43i1.41985

Abstract

Sweet cassava is tuber that contains large amount of starch so that it can produce reducing sugars. The addition of metal ions can increase enzyme activity. The purpose was to study the addition of Na and Ca ions on reducing sugars concentration and to study the hydrolysis kinetics. Experiments were carried out at various concentrations of substrates (100, 200, and 300 g/L), enzyme concentrations (1 and 1.5% (w/w)), addition of Na and Ca ions of 60 ppm. The hydrolysis method was conducted at temperature of 30°C with the enzyme StargenTM 002 at pH 4, for 24 hours. The results showed that starch concentrations of 100-200 g/L were able to significantly increase the reducing sugars at enzyme concentration of 1.5% (w/w). The addition of Ca ions increased the concentration of reducing sugar by 64% while the addition of Na ions increased it by 18.9%. The best reducing sugar concentration (68.79 g/L) was obtained at starch concentration of 200 g/L and enzyme concentration of 1.5% (w/w) with the of Ca ions. Kinetic studies show that phenomenon follows Michaelis-Menten equation with Vmax valued of 6.053; 8,881; 15.106 g/L.hour, respectively. So that, addition of Na and Ca ion can increase the enzyme activity
Composite Encapsulating Agent for NaFeEDTA Microencapsulation Using Spray Drying Method Handayani, Noer Abyor; Aryanti, Nita; Haryani, Kristinah; Hargono, Hargono; Adina, Alifia Rizki; Yuliani, Sari; Adristy, Vania Zulfa
Reaktor Volume 25 No.2 August 2025
Publisher : Department of Chemical Engineering, Faculty of Engineering, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/reaktor.25.2.%p

Abstract

Iron microencapsulation is one of the solutions to protect iron from reacting with other substances that result in quality declines of fortified food products. The coating materials used should be appropriate for the specified encapsulation process. This study combined glucomannan and maltodextrin as coating materials and added HPMC as an additive to encapsulate NaFeEDTA. The results showed that such a combination could increase the encapsulation efficiency. The morphologies of the resulting microcapsules obtained from four formulations applied in this study were identical; numerous wrinkles appear on the particle’s surfaces due to the presence of HPMC. All formulations had a narrow size distribution with an average particle size between 105 and 111 µm, thermal resistance up to a temperature of ± 200°C, and the same functional groups but with different intensities. The NaFeEDTA included in all formulations had a bioavailability value of 2.3 - 2.9%. The best formulation, based on the gastrointestinal digestion simulation, was shown by the formulation with a glucomannan:maltodextrin ratio of 2:3 (in 1% w/v coating material) with a release percentage of 64.28% in SGF solution and an increase of 2.27% in SIF solution. To prevent anemia, ± 400 mg of iron microcapsules resulting from the best formulation in this study are required to meet 75% of the daily intake.