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All Journal ARSHI Veterinary Letters Jurnal Kedokteran Hewan Jurnal Medika Veterinaria
Budianto Panjaitan
Laboratorium Klinik Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : ARSHI Veterinary Letters

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Gambaran histologi ovarium sapi aceh pascavitrifikasi menggunakan dimetyl sulfoksida dengan konsentrasi berbeda . Syafruddin; Arman Sayuti; Rahmat Aditya Sumardi; Budianto Panjaitan
ARSHI Veterinary Letters Vol. 2 No. 4 (2018): ARSHI Veterinary Letters - November 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.431 KB) | DOI: 10.29244/avl.2.4.77-78

Abstract

This study aims to determine the morphology of Aceh bovine ovarian pasca vitrification, after its been exposed to dimetyl sulfoksida (DMSO) cryoprotectant. This study use a completely randomized design of one-way pattern ANOVA acquire three replications. Ovaries used in this study are Aceh cattle ovarian which collected from slaughter house (RPH) accounted as 4 organs, to the next sliced into 9 pieces. Ovarian pieces are then grouped into 3 treatment groups namely ovaries which exposed into solution containing DMSO 10% (P1), 20% (P2), and 30% (P3). The results showed that the average number of normal follicles is highest at P1 41,33 ± 32,51; followed by P2 20,00 ± 16,09 , and P3 15,66 ± 10,50. It was concluded that  the ovarian tissue was exposed with DMSO 10% was able to maintain of the normal follicle than DMSO 20% and 30% although statistical test results showed no significant difference between group (P>0,05).
Gambaran histologis folikel ovarium sapi aceh pascavitrifikasi menggunakan etilen glikol Arman Sayuti; Jamilatun Hidayah; Muslim Akmal; Budianto Panjaitan
ARSHI Veterinary Letters Vol. 3 No. 1 (2019): ARSHI Veterinary Letters - Februari 2019
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.688 KB) | DOI: 10.29244/avl.3.1.3-4

Abstract

Penelitian ini bertujuan mengetahui gambaran histologis folikel ovarium sapi aceh pasca vitrifikasi menggunakan etilen glikol (EG) dengan berbagai konsentrasi berbeda yaitu 10%, 20% dan 30%. Phosphate buffered saline (PBS), 0.25 M sukrosa, 0.5 M sukrosa dan krioprotektan EG digunakan sebagai larutan vitrifikasi. Ovarium dipaparkan dengan larutan mengandung krioprotektan EG konsentrasi 10%, 20%, dan 30% selama 5 menit masing-masing pada suhu kamar, dikemas dalam straw, lalu divitrifikasi dalam nitrogen cair (-196 ⸰C), dan thawing dalam air pada 37 ⸰C. Kondisi normal dan keutuhan folikel pascavitrifikasi diamati secara histologi. Hasil pengamatan menunjukkan bahwa ovarium setelah vitrifikasi dengan EG 10% dan 20% memperlihatkan presentase keutuhan folikel 32.83% dan 45.04%, sedangkan EG 30% memperlihatkan presentase tertinggi yaitu 54.96%. Dari hasil penelitian ini dapat disimpulkan bahwa EG 30% lebih baik dalam mempertahankan keutuhan folikel ovarium sapi aceh dibandingkan dengan EG 10%  dan 20%.
Co-Authors . Syafruddin Amalia Sutriana Amiruddin A Arman Sayuti Cut Nila Thasmi Dasrul Dasrul Dian Nurcahaya Dwinna Aliza Erdiansyah Rahmi Ginta Riady Hamdan h Hamdan Hamdan Harizqi Fatwa Lingga Husnur rizal Ira Khubairoh Marpaung Ismail Ismail Jamilatun Hidayah Juli Melia maikhar gita eldora Mefrianti Efendi Muslim Akmal Rahmat Aditya Sumardi Razali Razali rina aulia barus Siti Aisyah Siti Rizki Hardyana Siregar Sulasmi Sulasmi Syafruddin S Syafruddin Syafruddin T. Armansyah Tongku Nizwan Siregar tongku nizwar siregar yusmadi y