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Penanganan Paraparesis Kaki Belakang pada Kucing Domestik dengan Metode Terapi Elektroakupunktur Mudhita Zikkrullah Ritonga; Cut Nila Thasmi; Sri Wahyuni; M. Nur Salim; M. Ikhsanuddin
Acta VETERINARIA Indonesiana Vol. 10 No. 1 (2022): Maret 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.1.51-57

Abstract

Paraparesis atau paresis merupakan suatu kondisi ditandai oleh lemahnya gerak badan, atau hilangnya sebagian gerakan badan atau adanya gangguan gerakan. Kucing domestik berumur ±4 bulan, berjenis kelamin jantan, ras kucing domestik campuran, warna rambut putih bercampur coklat muda, dengan berat badan 1,5 kg mengalami kelemahan pada kedua kaki belakang, tidak bisa berjalan disertai dengan kondisi tubuh yang tampak lemah serta lesu, dan tidak memberikan respon rasa sakit saat dilakukan penekanan pada regio femur dan digit sehingga menyebabkan ketidakmampuan pada kedua kaki belakang untuk mengangkat tubuh bagian belakang dengan sempurna sehingga menunjukkan adanya gangguan pada sistem saraf yang mempengaruhi anggota gerak dan muskuloskeletal. Hasil foto x-ray menunjukkan bahwa tidak adanya tulang yang fraktur dan pada organ visceral juga tidak menunjukkan perubahan ataupun kelainan yang signifikan. Namun terdapat suatu kondisi radiopaque dari perkembangan osteofit pada tulang belakang diantara L6-L7 sehingga menunjukkan diagnosa yaitu paraparesis kaki belakang. Terapi paraparesis kaki belakang pada kucing dengan metode elektroakupunktur dilakukan di titik BL-23, BL-25, BL-32 dan ST-36 pada tubuh kucing selama 4 minggu dengan durasi setiap terapi selama 15 menit yang dilaksanakan seminggu sekali selama 4 minggu. Kucing diberikan terapi Neurobion® injeksi 0,15 cc intra muscular setelah setiap terapi akupunktur dan diresepkan neurobion® sebanyak seperdelapan tablet satu kali sehari. Kesimpulannya yaitu metode terapi elektroakupunktur dengan titik akupunktur BL-23, BL-25, BL-32 dan ST-36 pada kucing yang mengalami paraparesis kaki belakang menunjukkan perkembangan yang membaik setelah dilakukannya terapi selama empat kali.
Perbandingan Kinerja Berahi dan Level Estradiol Kambing Kacang dan Kambing Nubian yang Diinduksi dengan PGF2α Mulyadi Adam; Raja Nurul Huda; Widya Zahara; Tongku Niswan Siregar; Sri Wahyuni; Cut Nila Thasmi; Rosmaidar Rosmaidar
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (10432.789 KB) | DOI: 10.22146/jsv.38444

Abstract

The experiment was conducted to compare estrous performance between and estradiol level kacang and Nubian goats estrous induction by prostaglandin F2 alpha (PGF2α). Eight female goats were divided into two groups, groups 1 (K1) consisted five Nubian goats and group 2 (K2) included three kacang goats with criterias as follows: age 1.5-3.0 years old, not pregnant, at least two months postpartum, and healthy clinically. All goats were estrous synchronized by double injection 7,5 mg PGF2α intramuscularly with 10 days interval. Goats that appeared estrus symptoms were mated naturally to a male goat. Determination of estrous intensity was performed using scoring method. Blood samples were taken at the first time the goats were mated (day 0). The measurement of estradiol concentration was done by enzyme linked immunosorbent assay (ELISA) method. Data of intensity, onset, duration, and estradiol level were analyzed using t test. The results showed that the rate of intensity, onset, duration, and estradiol level of estrous at KI vs KII were 2.08±0.52 vs 1.85±0.67, 56.00±34.11 vs 40.80±18.20 hours, and 24.00±26.15 vs 33.80±9.10 hours, and 103.51±42.49 vs 67.95±38.44 pg/ml (P>0,05). It can be concluded there was no difference of estrous performance and estradiol level between kacang and Nubian which estrous induction with PGF2α. The relationship between the intensity of estrus estradiol level in the Nubian goats was 0.995 while the kacang goats was 0.890.
Profil Biokimia Darah Sapi Aceh yang Mengalami Kawin Berulang Cut Nila Thasmi; Husnurrizal Husnurrizal; Muslim Akmal; Sri Wahyuni; Tongku Nizwan Siregar
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.013 KB) | DOI: 10.19087/jveteriner.2021.22.1.26

Abstract

Tujuan penelitian ini adalah mengetahui profil biokimia darah sapi aceh yang mengalami kawin berulang/repeat breeding (RB). Dalam penelitian ini digunakan 16 ekor sapi aceh yang terdiri atas tujuh ekor sapi aceh fertil dan sembilan ekor sapi aceh RB, yang berumur 3-8 tahun dengan skor kondisi tubuh (SKT) 3-4. Koleksi serum dilakukan untuk pemeriksaan profil biokimia darah meliputi kadar kolesterol, protein, dan glukosa. Kadar kolesterol; protein; dan glukosa sapi aceh fertil vs RB masing-masing adalah 185,86±45,34 vs 144,00±40,69 mg/dL; 6,57±1,55 vs 6,96±2,05 g/dL; dan 67,43±13,72 vs 73,78±15,83 mg/dL. Disimpulkan bahwa kadar glukosa sapi aceh RB lebih tinggi dibandingkan sapi fertile, sedangkan kadar kolesterol dan protein relatif sama.
THE POTENTIAL OF TOMATO EXTRACT (Lycopersicum esculentum L.) IN INCREASING THE SERTOLI CELLS AND SPERMATOGENIC CELLS COUNT OF THE CONTRALATERAL TESTICULAR SEMINIFEROUS TUBULES OF RATS (Rattus norvegicus) THAT HAVE TESTICULAR TORSION Dasrul Dasrul; Cut Nila Thasmi; Triva Murtina Lubis; Awaluddin Awaluddin; Winaruddin Winaruddin; Rasmaidar Rasmaidar; Yola Alifa
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (631.529 KB) | DOI: 10.21157/j.ked.hewan.v15i3.20720

Abstract

The purpose of this study was to analyze the potential of tomato extract (Lycopersicum esculentum L.) in increasing the Sertoli and spermatogenic cells count of the contralateral testis in white rats (Rattus novergicus) with unilateral torsion. A total of 24 male Wistar white rats, aged 3-4 months old with a weight of 180-200 g were used in this study. The rat samples were randomly divided into 4 treatment groups: Group 1 as a negative control (NC) which consisted of rats without testicular torsion and given tomato extract; Group 2 as a positive control (PC) which consisted of rats with unilateral testicular torsion 720° for 6 hours without given tomato extract; Group 3 as treatment 1 (T1) consisted of rats that were subjected to unilateral testicular torsion 720o for 6 hours and were given tomato extract at a dose of 100 mg/kg BW/day; and Group 4 as treatment 2 (T2) consisted of rats that were subjected to unilateral testicular torsion 720° for 6 hours and were given tomato extract at a dose of 200 mg/kg BW/day. Each group consisted of six rats. Tomato extract was administered orally for 30 days. The observations of the number of Sertoli cells and spermatogenic cells (spermatogonia cells, spermatocytes, and spermatids) were carried out histologically with Hematoxylin-Eosin (HE) staining and the slides were examined with a light microscope. The data obtained was analyzed by ANOVA and continued with Duncan's test. The results showed that the administration of tomato extract significantly increased (P0.05) the number of Sertoli cells and spermatogenic cells of the contralateral testicular seminiferous tubule. Giving tomato extract 100 mg/kg BW/day resulted in an increase in the number of Sertoli cells and better spermatogenic cells than tomato extract 200 mg/kg BW/day. In conclusion the administration of tomato extract after detorsion can increase the number of Sertoli cells and spermatogenic cells of the contralateral testicular seminiferous tubules in white rats subjected to unilateral torsion of 720° for 6 hours
THE IMPROVEMENT OF SEMEN QUALITY AND TESTOSTERONE LEVEL OF BALI CATTLE AFTER PROSTAGLANDIN F2α ADMINISTRATION Eka Meutia Sari; Satria Tanjung; Dewi Ratna Sari; Muslim Akmal; Tongku Nizwan Siregar; Cut Nila Thasmi
Jurnal Kedokteran Hewan Vol 13, No 4 (2019): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.178 KB) | DOI: 10.21157/j.ked.hewan.v13i4.14821

Abstract

This study aimed to determine the effect of prostaglandin F2α (PGF2α) administration on improving semen quality and testosterone levels on Bali cattle. This study used 3 Bali cattle aged 2, 3.5, and 5 years old. The sample used in this study was Bali cattle semen. In this study, the Latin Square Design was used with three different treatments were administered over three time periods. The treatments performed were P1 (5 mL physiological NaCl), P2 (25 mg prostaglandin), and P3 (37.5 mg prostaglandin) while treatment period was three weeks of treatment administration, which were 1st. 2nd, and 3rd week. Blood collected through coccygea vein 30 minutes after treatment to measure testosterone levels using enzyme-linked immunosorbent assay (ELISA) technique. Statistical analysis showed that PGF2α administration at a dose of 25 mg and PGF2α at a dose of 37.5 mg had no effect (P0.05) on volume, color, pH, consistency, concentration, and motility of Bali cattle spermatozoa. The volume of semen obtained from P1; P2; and P3 were 6.5±0.9; 6.3±0.6; and 6.2±1.2 mL, respectively. The color of the semen in P1, P2, and P3 were beige and milky white. The pH in groups P1; P2; and P3 were 6.7±0.6; 7.1±0.2; and 6.5±0.2, respectively. Spermatozoa concentration in P1; P2; and P3 were 1,328±96.43 x 106 cells/mL; 1,354±102.19 x 106 cells/mL; and 1,353.3±88.55 x 106 cells/mL, respectively. Spermatozoa motility in P1; P2; and P3 were 73.3±2.8%; 71.6±2.8%; and 73.3±2.8%, respectively. Testosterone levels in P1 and P3 were 5.05±0.22 and 6.74±1.38 ng/mL, respectively (P0.05). Based on the results of this study, it was concluded that the administration of PGF2α does not affect semen quality but can increase the level of testosterone on Bali cattle.
DETECTION OF SERUM MACRO MINERALS CONCENTRATION IN REPEAT BREEDING ACEH CATTLE Cut Nila Thasmi; Husnurrizal Husnurrizal; Muslim Akmal; Sri Wahyuni; Tongku Nizwan Siregar
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.373 KB) | DOI: 10.21157/j.ked.hewan.v14i2.16543

Abstract

The purpose of this study was to determine the macro minerals concentration in serum of repeat breeding (RB) Aceh cattle. In this study 16 Aceh cattle were examined; they consisted of 7 fertile Aceh cattle (P1) and 9 Aceh cattle with RB (P2), all of which were 3-8 years old with abody condition score (BCS) of 3-4. Serum collection was carried out for examining the level of serum minerals, including magnesium, phosphorus, sodium, potassium, chloride, and calcium. Data was analysed using T-test. The levels of magnesium, phosphorus, sodium,potassium, chloride, and calcium in P1 vs P2 were 2.18±0.60 vs. 2.20±0.34 mg/dL; 6.18±1.34 vs. 6.48±0.74 mg/dL; 142.71±5.09 vs.142.44±2.29 mmol/L; 4.81±0.76 vs. 4.76±0.51 mmol/L; 104.57±4.35 vs. 107.67±7.36 mmol/L; and 9.07±0.45 vs. 9.90 ± 0.60 mg/dL (P0.05), respectively. It was concluded that the concentration of serum macro minerals do not affect the incidence of RB in Aceh cattle.
ANGKA KEBUNTINGAN SAPI LOKAL SETELAH DIINDUKSI DENGAN PROTOKOL OVSYNCH (Conception Rates of Local Cows after Induction with Ovsynch Protocols) Mefrianti Efendi; Tongku Nizwan Siregar; Hamdan Hamdan; Dasrul Dasrul; Cut Nila Thasmi; Razali Razali; Arman Sayuti; Budianto Panjaitan
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3804

Abstract

This study aims to know the effect of estrus synchronization using ovsynch method on local cow pregnancy rate. This study used 10 local cows without pregnant status and clinically healthy. All cows were divided into 2 treatment groups. Cows in group I (K1) were synchronized estrus using 5 mg/ml PgF2α intramuscularly with multiple injection patterns with intervals of 10 days. Group II (KII) was synchronized by ovsynch protocol, initiated with injection of 1ml GnRH on day-0. On the 7th day, the whole cows in K II were injected with 2 ml PgF2α then followed by re-injected with 1 ml of GnRH on the 9th day. Artificial insemination was performed 24 hours after GnRH last injection. The data were analyzed descriptively. The results showed that 3 cows in group I were diagnosed pregnant (60%), whereas in group II, 4 cows werediagnosed pregnant (80%). In conclusion, the estrus synchronization with ovsynch protocols provide a higher pregnancy rate than the conventional estrus synchronization.Key words: synchronization, ovsynch, GnRH, PgF2α
30. Quality of Simeulue buffalo spermatozoa after freezing with AndroMed®diluent Zamma Khasyarif; Dasrul Dasrul; Ginta Riady; Cut Nila Thasmi; Azhar Azhar
Jurnal Medika Veterinaria Vol 13, No 2 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i2.3364

Abstract

The study aims to determine the effect of diluent concentration Adromed® against the quality of spermatozoa of simeulue buffalo following freezing process. This study uses a completely randomized design (CRD) with 3 treatment groups. Group 1  used AndroMed® diluent 15%; Group 2 with  AndroMed® 20%; and, Group 3 with AndroMed® 25%. Each group was repeated 3 times. Quality of spermatozoa assessed includes the motility and viability of spermatozoa. The data on the quality results  were analyzed by analysis of variance (ANOVA)  in one direction followed by Duncant test. The average percentage of motility after freezing were found in the group P1, P2 and P3 respectively is 32.60 ± 3.27 %; 41.69 ± 4.14 %  and 47.58 ± 3.11%; spermatozoa viability is 36.56 ± 3.42%; 47.77 ± 4.81 % and 51.38 ± 3.89%. Statistical analysis showed that the concentration of diluent AndroMed® significantly affected (P 0.05) the motility and the percentage of live sperms. The percentage of live sperm motility and the live sperms did not differ significantly between G1 with G2 treatment groups and both are significantly different (P0.05) compared with group G3. AndroMed®  concentration affects the quality of simeulue buffalo spermatozoa after freezing. AndroMed® concentration of 20% and 25% were improved the quality of buffalalo spermatozoa following freezing compared with that with AndroMed® 15% .
9. The Effectiviteness of Jatropha salve (Jatropha curcas L) in Inflammatory Phase of cutaneous wound healing in Mice (Mus musculus) Histopathologically Indra Sitorus; M Nur Salim; Nazaruddin Nazaruddin; Dwinna Aliza; Denny Irmawati; Awaluddin Awaluddin; Cut Nila Thasmi; Muslim Akmal; Dian Masyitha
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.4374

Abstract

This study aims to determine the effectiveness of 10% Jatropha salve (Jatropha curcas L. )    to inflammation reaction cutaneous wound healing in mice (Mus musculus ) in the inflammatory phase. This experiment used 9 male mices, weighing 25-40 grams and age 2-3 months old, were divided into three treatment groups, each treatment consisting of three mices. The wound were made along 2 cms in the back area. Wound therapy was carried out  twice a day for 3 days.  Group KI was administered  and given by yellow vaseline, KII was given 10% Jatropha salve, and KIII was given gentamicin salve. The parameters measured are the increase in the number infiltrations of inflammatory cells,  neovascular, and re-epitelization. All quantitative data were measured using ANOVA and continued with Duncan Test, the qualitative data were presented descriptively by showing the results of observation under a microscope. The statistical test on the infiltrations of inflammatory cells and neovasculars parameter 10% Jatropha salve group significantly different (P0.05) compared to KI and KIII group. Histopatologycal observations indicated the 10% Jatropha salve could be reduce the infiltrations of inflammatory cells, increase neovasculars and re-epitelization percentage. According to the result can be concluded the 10% Jatropha salve can accelerate the inflammation phase cutaneous wound healing in mice.
PENGARUH PEMBERIAN PMSG DAN HCG TERHADAP PENINGKATAN JUMLAH KELAHIRAN HAMSTER CAMPBELL (Phodopus campbelli) (Effect of PMSG and hCG to Increase Birth Number in Campbell’s Hamsters (Phodopus campbelli)) Novi Afriani Nur Afriani Nur; Tongku Nizwan Siregar; Hamdan Hamdan; Idawati Nasution; Cut Nila Thasmi; Dasrul Dasrul
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i1.4041

Abstract

The aim of this study was to determine the effect of superovulation induction through Pregnant Mare’s Serum Gonadotropin (PMSG) and Human Chorionic Gonadotropin (hCG) injection to increased birth number in Campbell’s hamsters (Phodopus campbelli). This study used 10 unpregnant female Campbell’s hamster, aged 4-6 months, weighing of 25-30 grams. Hamsters were divided into K1 and K2, each consist of five hamsters. K1 injected with 1 ml physiologic NaCl, while K2 intraperitoneally injected with PMSG and hCG in 5 IU. Mating was held after injection. Male and female hamsters were put in one box with ration of 1:1. The observation of vagina plug was done in order to confirm whether the mating successes or not. The average amount of fetus birth through superovulation on K1 and K2 were 3.40±1.3 and 7.80±1.1, respectively. In conclusion, the superovulation induction using the combination of PMSG and hCG increase birth number on Campbell’s hamsters.