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Spermatogenesis and semen quality of male muntjak (Muntiacus muntjak muntjak) during antler growth periods Sri Wahyuni; Srihadi Agungpriyono; Muhammad Agil; Idawati Nasution; . Hamny; Tuty L. Yusuf
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (512.016 KB)

Abstract

Muntjak (Muntiacus muntjak muntjak) belongs to Cervidae family which distributed in Java Island and Southern part of Sumatera. This cervid has been protected by Indonesian Government since 1999. In order to support breeding program of the species and to avoid them from extinction, itsreproductive biology such as spermatogenesis and the correlation to semen quality is important to be investigated. Therefore, the objective of this study was to examine spermatogenesis and semen quality of two adult male muntjaks during antler growth periods that consist of hard antler (H), casting (C), andvelvet antler (V). Testicular tissues and semen (ejaculates) were obtained by core needle biopsy and electroejaculation methods respectively. Testicular tissues were processed histologically and stained with periodic acid Schiff (PAS) to observe spermatogenesis whereas semen was evaluated to obtaine itsquality. The results showed that spermatogenic activities were detected in all antler periods which marked by PAS positive staining (magenta colour) of round and elongated spermatid acrosomes. In H period, spermatogenic activity was higher than those C and V periods. According to semen evaluation,motile spermatozoa were found with different concentration in all antler periods. The highest sperm concentration (x 106 spermatozoa/ml) in both of muntjaks was found in H period (506.25 ± 61.87), and slightly decreased in C (288.75 ± 37.12), and V periods (362.60 ± 17.68). These finding showed thatspermatogenesis to produce spermatozoa is taken place while muntjaks are in C and V periods with differ activities that provable with the existence of motile spermatozoa from ejaculates in both of male muntjaks. Therefore, muntjaks could provide reproductive function throughout the year of reproductiveaseasonality which is similar to the reeves and formosan muntjaks
RASIO KETEBALAN DINDING TERHADAP DIAMETER TULANG HUMERUS AYAM KAMPUNG (Gallus domesticus) DAN BURUNG MERPATI (Columba domestica) Idawati Nasution; Shinta Mutia RM; Hamny -
Jurnal Medika Veterinaria Vol 7, No 1 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v7i1.2264

Abstract

Penelitian ini bertujuan mengetahui perbandingan ketebalan dinding terhadap diameter tulang humerus pada ayam kampung (Gallus domesticus) dan burung merpati (Columba domestica). Dua puluh tulang humerus yang berasal dari 10 ekor burung merpati jantan dipisahkan dari jaringan di sekitarnya, kemudian tulang humerus tersebut direndam dalam formalin 5% dan dipotong secara melintang dengan menggunakan gergaji tulang. Ketebalan dinding terhadap tulang humerus diukur dengan menggunakan jangka sorong. Hasil penelitian dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa perbandingan ketebalan dinding dan diameter tulang humerus pada ayam kampung adalah 1 : 4,94 sedangkan pada burung merpati adalah 1 : 5,80. Tulang humerus ayam kampung lebih tebal dari pada burung merpati jika dibandingkan diameter tulang dari masing-masing unggas tersebut.Kata kunci: Gallus domesticus, Columba domestica, tulang humerus, rasio, diameter, ketebalan
INTERPRETASI UKURAN JANTUNG ORANGUTAN SUMATERA(Pongo abelii) BERDASARKAN FOTO RONTGEN TORAKS DI PUSAT KARANTINA ORANGUTAN SUMATERA UTARA Qaida Minati; Arman Sayuti; Idawati Nasution
Jurnal Medika Veterinaria Vol 7, No 2 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v7i2.2936

Abstract

Penelitian ini bertujuan mengetahui ukuran jantung orangutan sumatera (Pongo abelii) berdasarkan foto rontgen toraks dengan proyeksi posterior anterior. Foto yang digunakan adalah foto rontgen orangutan sehat sebanyak 48 ekor di Pusat Karantina Orangutan Sumatera Utara yang terdiri atas 24 ekor jantan dan 24 ekor betina yang merupakan sitaan dari Mei 2003 hingga November 2011.  Foto rontgen dikelompokkanberdasarkan jenis kelamin dan tingkatan umur. Pengukuran ukuran jantung dilakukan berdasarkan foto rontgen toraks dan selanjutnya  dilakukanperhitungan dengan cardiothoracic ratio (CTR). Nilai rata-rata CTR pada orangutan jantan dengan kelompok umur  bayi (0-2,5 tahun), anak(2,5-7 tahun), remaja (7-10 tahun) masing-masing adalah 45,2;  41,6; dan 38,8% sedangkan untuk orangutan betina masing-masing adalah 45,6;42,1; dan 40,3%.  Bayi orangutan memiliki nilai CTR yang paling besar.  Cardiothoracic ratio (CTR) menurun pada setiap peningkatan umurdari kedua jenis kelamin
PREVALENSI PERUBAHAN PERILAKU ANJING LOKAL (Canis familiaris) JANTAN YANG DIKANDANGKAN DENGAN PRINSIP KESEJAHTERAAN HEWAN SELAMA 60 HARI (Prevalence of Behavioural Changes in Male Local Dog (Canis familiaris) Caged Based on Animal Welfare Principles for 60 Days) Nofri Alfi; Teuku Reza Ferasyi; Erdiansyah Rahmi; Mulyadi Adam; Idawati Nasution; Ismail Ismail
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3823

Abstract

The aim of this research was to determine the prevalence of behavioral changes of male local dog caged based on animal welfare principles. A total of three male local dogs was used as the experiment object. Dog behavior was observed using focal animal sampling method. Dogs were caged for 60 days, provided with sufficient food and water ad libitum. The dogs were identified based on their color and body size. Their behavior were observed three times each days in the morning, midday, and afternoon with 3 periods: period I (day 1 to day 20), period II (day 21 to day 40), and period III (day 41 to day 60). The observation was carried out for 60 days with allocation time was 30 minutes/ each observations. The result obtained indicated that the prevalence of behavior in dogs during the first period was dominated by sitting and sleeping. In addition, they were showed the behavior of running, walking, playing, sniffing, and eating. During this period, they were also expressed of stress behavior, such as barking, digging, backward/hiding, biting, and climbing. In the second and third periods of observation, the behaviors of sitting and sleeping are increased while behaviors of stress are reduced. Based on this results, it can be concluded that local dogs indicate stress behavior during the first 20 days caged, then they become more calm during the following days when animal welfare principle is fulfilled.Key words: local dog, dog behavior, animal welfare
TINJAUAN ASPEK KESEJAHTERAAN HEWAN PADA SAPI YANG DIPOTONG DI RUMAH PEMOTONGAN HEWAN KOTAMADYA BANDA ACEH (Study of the Animal Welfare Aspect on Cattle Slaughtered in Slaughter house in Banda Aceh) Yudha Bhaskara; Mulyadi Adam; Idawati Nasution; Triva Murtina Lubis; Teuku Armansyah; Muhammad Hasan
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3806

Abstract

This study was conducted to obtain the information about animal welfare on cattle slaughtered in Slaughterhouse in Banda Aceh. The animal welfare parameter was observed related to the transport aspect, shelter aspect, and slaughter aspect. The three aspects compared to the recommendation of Meat Livestock Australia (MLA) and Indonesian National Standard 02-4509-1998. Method used to determine the animal welfare was scoring evaluation. The result of study showed that transport aspect get 20 score with a percentage of 50% which categorized as sufficient. Shelter aspect get 33 score with percentage of 82,5% which categorized as good, and slaughter aspect get 32 score with percentage of 80% which categorized as good. Based on scoring evaluation of shelter aspect and slaughter aspect, the animal welfare of cattle slaughtered in Slaughter house in Banda Aceh is considered good, meanwhile the transport aspect is considered sufficient.Key words: animal welfare, cattle, slaughterhouse
EFEK EKSTRAK DAUN PEGAGAN (Centella asiatica (L.) Urban) TERHADAP PERKEMBANGAN SEL SPERMATID TIKUS (Rattus norvegicus) Wahyu Sihombing; Muslim Akmal; Sri Wahyuni; Idawati Nasution; Rinidar -; Hamdan -
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i1.3003

Abstract

Penelitian ini bertujuan mengetahui efek pemberian ekstrak daun pegagan terhadap perkembangan sel spermatid tikus. Penelitian ini merupakan penelitian eksperimental dengan menggunakan rancangan acak lengkap (RAL) pola one way analysis of varian (ANOVA). Tikus yang digunakan adalah tikus jantan berumur 3,5 bulan dengan bobot badan 150-250 g sebanyak 12 ekor tikus. Tikus-tikus tersebut dikelompokkan menjadi empat kelompok perlakuan dengan masing-masing tiga ulangan. Kelompok pertama merupakan kelompok kontrol (K0) yang tidak diberi perlakuan, sedangkan kelompok K1, K2, dan K3 diberi perlakuan berupa ekstrak daun pegagan dengan dosis bertingkat, yaitu 125, 250, dan 500 mg/kg bobot badan selama 30 hari. Pada akhir perlakuan, tikus dikorbankan dengan diberi eter, lalu testis dinekropsi untuk selanjutnya dibuat preparat histologis.Preparat histologis diwarnai dengan pewarnaan periodic acid Schiff (PAS) untuk mengetahui perkembangan sel spermatid dengan cara mendeteksi karbohidrat netral pada tudung akrosom sel spermatid tersebut. Data dianalisis dengan ANOVA dan dilanjutkan dengan uji Tukey HSD. Hasil penelitian memperlihatkan bahwa rataan jumlah sel spermatid mengalami penurunan dibandingkan kontrol (K0). K0 mempunyai rataan jumlah sel spermatid tertinggi yaitu 308,00±56,33; diikuti oleh K1 yaitu 234,38±19,81; K2 yaitu 218,50±5,48; dan K3 yaitu 208,05±27,35. Hasil uji statistikmenunjukkan adanya pengaruh yang signifikan (P0,05) antara dosis dan rataan jumlah sel spermatid antara K0 dengan K2 dan K0 dengan K3. Kesimpulan dari penelitian ini adalah ekstrak daun pegagan dapat mengurangi laju perkembangan sel spermatid tikus.
GANGLION SIMPATIS PADA KAMBING LOKAL (Capra sp.) Idawati Nasution; Nanda Yuliansyah; Hamny -
Jurnal Medika Veterinaria Vol 7, No 1 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v7i1.2911

Abstract

Penelitian ini bertujuan mengetahui struktur anatomi dan letak ganglion simpatis pada kambing lokal (Capra sp.).  Penelitian ini menggunakan empat ekor kambing (dua ekor jantan dan dua ekor betina), rata-rata berumur 2 tahun. Struktur anatomi ganglion dilakukan dengan cara inspeksi dan palpasi setiap ganglion yang diamati. Letak ganglion dideskripsikan berdasarkan letak ganglion terhadap buluh darah atau nervus yang berada di sekitar ganglion. Hasil pengamatan dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa terdapat empat ganglion dari syaraf simpatis pada kambing lokal yaitu ganglion cervicale cranial yang terletak di dorsal arteri occipitalis dan di caudo-medial nervus vagus. Ganglion cervico-thoracicum/stellatum berada di cranial ramus communicantes, di caudal arteri dan vena costocervicovertebralis, di lateral trakea dan di ventral nervus vertebralis. Ganglion celiaco-mesentericum/solare terletak di dorsal arteri mesenterica cranialis dan caudal arteri celiaca. Ganglion mesentericum caudale terletak di antara arteri umbilicalis dextra dan sinistra serta di dorsal arteri sacralis mediana.Dsimpulkan bahwa ganglion celiaco-mesentericum/solare memiliki struktur anatomi yang berbeda dibandingkan dengan ketiga ganglion simpatis lainnya pada kambing lokal