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Triva Murtina Lubis

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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Characteristics of frozen-thawed semen on Simmental and Limousin bulls in Ungaran, Indonesia Triva Murtina Lubis; Prabowo Purwono Putro; Aris Junaidi; . Hamny
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (89.668 KB)

The present research aimed to study the characteristics of frozen-thawed semen in beef bulls ex-import in Ungaran, Indonesia. 5 heads Simmental and 5 heads Limousin of 5.5 years old were used in this research. The research was done in Ungaran Artificial Insemination Center, Central Java, Indonesia and Laboratory of Reproduction and Obstetry, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia. Frozen semen was obtained from this AI center. Results of the research indicated that the average individual motility of Simmental and Limousin is 39.00±5.48% and 36.00±2.24%. The average percentage of live spermatozoa is 54.00±10.75% for Simmental and 48.20±9.78% for Limousin while the average proportion of abnormal spermatozoa is 9.60±3.36% and 6.80±4.15% for both Simmental and Limousin, respectively. On average, Simmental frozen-thawed semen have the higher mean proportion of sperm motility, live sperm, and abnormal sperm than that of Limousin frozen-thawed semen

Hematology profile of Sumatran Orangutan (Pongo abelii) in the Sumatran Orangutan Quarantine Center, Sibolangit, Indonesia Maiyus Musrianti; Triva Murtina Lubis; Ahmad Damhoeri
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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This research aims to obtain hematological database of healthy Sumatran orangutans (Pongo abelii) at the Sumatran Orangutan Quarantine Centre, in Batu Mbelin, Sibolangit, North Sumatra, Indonesia. This secondary datacomes from hematological examination results in 52 healthy Sumatran orangutans in Batu Mbelin, from May 2003 to August 2010. This research used a factorial complete randomized design (CRD) that has two factors namely, gender as the first factor and age as the second factor. Data was analyzed using analysis of variance (ANOVA). The results of ANOVA show that gender affects (P0.05) on the value of mean cell hemoglobin concentration (MCHC) in Sumatran orangutans. Age influences (P0.05) on the lymphocytes and monocytes Sumatran orangutans. The results of least significant different (LSD) test of the lymphocyte show that there are significant differences (P0.05) between the lymphocyte value of infants and that of juveniles. The lymphocyte value of infant is also significantly different (P0.05) that of adolescent. While the lymphocyte value of adolescent and juveniles are not significant different. The results of LSD test of the value of monocytes show that there is a very significant difference (P0.01) between the monocyte value of infants and that of juveniles. Furthermore, the value of infant’s and adolescent’s monocytes issignificantly different (P0.05). However, the value of adolescent’s and juvenile’s monocytes does not show any influences. Interaction of sex influences on the red blood cell (RBC) of Sumatran orangutans whereas age do notinfluences on the RBC. The results of Duncan test show that there are significant differences (P0.05) between the RBC values of adolescent female orangutans and adolescent male orangutans. Other hematological values do not show any effects on both gender and age

UJI ANTIBAKTERI EKSTRAK DAUN KELOR (Moringa oleifera L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus (Antibacterial Activity Test of Moringa oleifera L. Extracts on Staphylococcus aureus) Elza Savitri; Fakhrurrazi Fakhrurrazi; Abdul Harris; Erina Erina; Amalia Sutriana; Triva Murtina Lubis
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun kelor (Moringa oleifera L.) terhadap pertumbuhan bakteri Staphylococcus aureus. Ekstrak daun kelor diekstraksi dengan cara maserasi yaitu menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar menggunakan kertas cakram yang telah direndam dalam ekstrak etanol daun kelor dengan konsentrasi 20%, 40%, 60% dan 80% pada media Mueller Hinton Agar (MHA). Parameter yang diukur adalah besarnya diameter zona hambat yang terbentuk disekitar kertas cakram. Hasil uji aktivitas antibakteri dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kelor memiliki daya hambat terhadap bakteri Staphylococcus aureus yaitu pada konsentrasi 80% sebesar 14,02 mm (kategori kuat), 60% sebesar 12,03 mm (kategori kuat), 40% sebesar 9,00 mm (kategori sedang), 20% sebesar 7,98 mm (kategori sedang), kontrol positif 28,63 mm (kategori sangat kuat) dan kontrol negatif tidak menunjukkan efek antibakteri. Dapat disimpulkan bahwa ekstrak daun kelor memiliki antibakteri terhadap pertumbuhan bakteri Staphylococcus aureus. The aim of this research is to observe the antibacterial activity of Moringa oleifera L. extract on Staphylococcus aureus. Moringa oleifera extract was extracted by maceration using ethanol 96%. The antibacterial activity test was carried out using diffusion method used paper disc which has been soaked in etanol extract of Moringa oleifera with concentration of 20%, 40% and 60% on Mueller Hinton Agar (MHA). Parameters measured were the diameter of inhibition zone formed around the paper disc. The results of antibacterial activity test were analyzed descriptively. The result showed that etanol extract of Moringa oleifera had inhibitory on Staphylococcus aureus. Moringa oleifera extract had inhibitory on Staphylococcus aureus at concentration 80% was 14,02 mm (strong category), 60% was 12,03 mm (strong category), 40% was 9,00 mm (middle category), 20% was 7,98 mm (middle category), positive control was 28,63 mm (very strong category) and negative control did not show antibacterial effect. It can be concluded that Moringa oleifera L. extract has an antibacterial effect on Staphylococcus aureus.

INTEGRITAS MEMBRAN PLASMA SPERMATOZOA SAPI ACEH PASCA PEMBEKUAN DALAM MEDIA SITRAT KUNING TELUR DENGAN WAKTU EKUILIBRASI YANG BERBEDA (The Plasma Membrane Integrity Of Post-Freezing Aceh Cattle Spermatozoa In Yolk-Citrate Medium In Different Equilibration Times) nikhofebri hidayat; dasrul dasrul; hamdan hamdan; husnurrizal husnurrizal; muslim akmal; triva murtina lubis
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Penelitian ini bertujuan untuk mengetahui pengaruh waktu ekuilibrasi terhadap integritas membran plasma spermatozoa sapi aceh pasca pembekuan dengan media sitrat kuning telur. Sampel yang digunakan adalah semen sapi segar yang dikoleksi menggunakan vagina buatan. Sampel semen dikoleksi dari 1 ekor sapi aceh jantan sehat berumur 3 tahun sebanyak satu kali dalam seminggu selama lima minggu. Semen yang berkualitas baik diencerkan dengan pengencer sitrat kuning telur. Selanjutnya dibagi menjadi 5 kelompok perlakuan dengan waktu ekuilibrasi yang berbeda-beda (K1); 2 jam (K2); 3 jam (K3); 4 jam (K4); 5 jam (K5); 6 jam dan masing-masing kelompok diulangi sebanyak 5 kali. Kemudian dilakukan pembekuan dengan medote vitrifikasi di atas uap nitrogen cair (suhu sekitar (-110 ºC) selama 13 menit dan disimpan di dalam kontainer berisi nitrogen cair (-196ºC). Setelah penyimpanan selama 1 minggu, masing-masing semen beku dilakukan thawing dan selanjutnya dihitung persentase membran plasma utuh (MPU) setiap straw perlakuan. Data yang diperoleh dianalisis menggunakan analysis of variance (ANOVA) dan dilanjutkan dengan uji Duncan. Rata-rata MPU spermatozoa sapi aceh pada media sitrat kuning telur K1; K2; K3; K4 dan K5adalah 34,52±4,59%; 52,06±5,86%; 57,24±5,07%; 48,22±3,70% dan 5,44±1,07%. Hasil penelitian menunjukkan waktu ekuilibrasi berpengaruh nyata (P0,05) terhadap persentase MPU spermatozoa sapi aceh. Penggunaan waktu ekuilibrasi 4 jam menghasilkan MPU spermatozoa sapi aceh yang lebih baik dibandingkan lama waktu ekuilibrasi lainnya pasca pembekuan. (This study is aimed to determine the effect of equilibration time on the integrity of aceh cattle spermatozoa plasma membrane. The sample used was fresh cattle semen collected using artificial vagina. The semen samples were collected from one healthy cattle aged three years old once in a week during five weeks. Good quality semen was divided into five treatment groups with different equilibration times which are (K1); 2 hours (K2); 3 hours (K3); 4 hours (K4); 5 hours (K5); 6 hours and the collecting process was repeated five times for each group. Then, the samples were frozen by vitrification method on the top of liquid nitrogen vapor (ambient temperature (-110 ºC)) for 13 minutes and stored in containers of liquid nitrogen (-196ºC). After one week of storage, each frozen semen was thawed and the percentage of the intact plasma membrane (MPU) was calculated for each treatment of straw. The data obtained were analyzed by using analysis of variance (ANOVA) and continued by using Duncan test. The mean of MPU aceh cattle spermatozoa on citrate yolk medium K1; K2; K3; K4 and K5 are 34,52±4,59%; 52,06±5,86%; 57,24±5,07%; 48,22±3,70% and 5,44±1,07%. The study results showed equilibration times have a real impact (P0,05) on the of MPU aceh cattle spermatozoa. The use of a 4-hour equilibration time resulted in a better percentage of MPU of aceh cattle spermatozoa compared to other post-freezing equilibration times.)

ANALISIS KONSENTRASI HORMON IGF-1 CAIRAN FOLIKEL OVARIUM SAPI ACEH DARI LIMBAH RUMAH POTONG HEWAN (RPH) BANDA ACEH. (Analysis of IGF-1 Hormone Concentration in Ovary Follicular Fluid of Aceh Cow from Animal Slaughterhouse in Banda Aceh) Nabilah Putroe Agung; Mulyadi Adam; Gholib Gholib; Juli Melia; Ummu Balqis; Triva Murtina Lubis
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 2 (2022): FEBRUARI-APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i2.7873

Penelitian ini bertujuan untuk mengukur konsentrasi hormon IGF-1 pada cairan folikel ovarium sapi aceh. Cairan folikel diaspirasi dari 30 pasang ovarium yang dikoleksi dari 30 ekor sapi aceh di Rumah Potong Hewan (RPH) Banda Aceh. Cairan ovarium dikoleksi berdasarkan ukuran folikel dan diidentifikasi ada tidaknya korpus luteum (CL). Ukuran folikel dikategorikan menjadi tiga (3) kategori yaitu folikel kecil (Ø1˗˗5 mm), folikel sedang (Ø5˗˗8,5 mm), dan folikel besar (Ø≥8,5 mm). Pengukuran konsentrasi hormon IGF-1 dilakukan dengan metode Enzyme Linked Immunosorbent Assay (ELISA). Data dianalisis menggunakan uji Kruskal-Wallis. Hasil penelitian menunjukkan bahwa sebanyak 12 ovarium (40%) memiliki CL dan 18 ovarium (60%) tidak memiliki CL. Konsentrasi hormon IGF-1 pada ovarium yang memiliki CL dengan kategori folikel kecil dan sedang masing-masing 17,39±7,24 ng/ml dan 0,1 ng/ml. Konsentrasi hormon IGF-1 pada ovarium tanpa CL dengan kategori folikel kecil, sedang, dan besar masing-masing 8,81±2,73 ng/ml, 2,09±0,27 ng/ml, 1,38±0,93 ng/ml. Rataan IGF-1 pada folikel kecil terlihat lebih tinggi baik pada ovarium yang memiliki CL maupun pada ovarium yang tidak memiliki CL, namun secara statistik tidak berbeda (p0,05). Dapat disimpulkan bahwa konsentrasi hormon IGF-1 pada semua kategori folikel tidak berbeda baik pada ovarium yang memiliki CL maupun ovarium yang tidak memiliki CL. (This study was conducted to measure concentration of IGF- hormone in ovary follicular fluid of aceh cow. Follicular fluid was aspired from 30 ovarian pairs collected from 30 aceh cows in Banda Aceh animal slaughterhouse. Ovarian fluids are collected based on the follicle size and identified by the presence of the corpus luteum (CL). The size of the follicle are categorized in three categories: small (Ø1˗˗5 mm), medium (Ø5˗˗8.5 mm), and large (Ø≥8.5 mm) follicles. Measurement of IGF-1 concentration using an Enzyme Linked Immunosorbent Assay (ELISA) method. Data were analyzed using a Kruskal-Wallis test. The results showed that 12 ovaries (40%) have CL and 18 ovaries (60%) have not CL. The concentrations of IGF-1 in the ovaries that have CL in small and medium follicles were 17.39±7.24 ng/ml and 0.1ng/ml, respectively. The concentrations of IGF-1 in the ovaries without CL in small, medium, and large follicles were 8.81±2.73 ng/ml, 2.09±0.27 ng/ml, 1.38±0.93 ng/ml, respectively. The mean of IGF-1 concentration in small follicles was higher in both ovaries with CL or without CL, but it was not significantly different (p0.05). It can be concluded that the concentration of IGF-1 in all categories of follicles did not differ either in ovaries with CL and without CL.)

GAMBARAN LEUKOSIT KELINCI SETELAH PEMASANGAN IMPLAN PLATE MATERIAL LOGAM(PARAMETER OF RABBIT LEUKOCYTE AFTER INSTALLATION METAL PLATE IMPLANT) HEFRI YUNALDI; erwin erwin; nuzul asmilia; sugito sugito; triva murtina lubis; al azhar
JURNAL ILMIAH MAHASISWA VETERINER Vol 3, No 2 (2019): FEBRUARI-APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

ABSTRAKLeukosit adalah sel pertahanan tubuh yang berfungsi memfagosit seluruh benda yang dianggap asing oleh tubuh. Penelitian ini bertujuan untuk mengetahui jumlah total leukosit dan persentase diferensial leukosit kelinci setelah pemasangan implant plate material logam. Penelitian ini menggunakan 6 ekor kelinci yang dibagi menjadi 2 kelompok. Kelompok I (K-I) diimplan plate tantalum dan kelompok II (K-II) diimplan plate besi. Pengambilan darah melalui vena auricularis pada hari ke 0, 7, 14, 28, dan 56 setelah implan. Hasil penelitian menunjukkan implan plate tantalum dan plate besi tidak berpengaruh terhadap total leukosit dan diferensial leukosit. Total leukosit diantara hari pengamatan menunjukan perbedaan pada masing-masing kelompok, namun masih dalam nilai normal. Penggunaan implan plate tantalum dan plate besi tidak memiliki efek terhadap gambaran sel darah putih dan persentase diferensial leukosit.Kata kunci : leukosit, diferensial leukosit, plate besi, plate tantalumABSTRACTLeukocytes are the body's defense cells that function to phagocyte all objects considered as foreign by the body. This study aims to determine the total leukocytes and differential percentage of rabbit leukocytes that paired of plate implants with metal material. This study used 6 rabbits divided into 2 groups. Group I was implanted with tantalum plate and group II was implanted using an iron plate. Blood collection through the auricular vein on days 0, 7, 14, 28, and 56. Leukocyte and differential counts leukocytes are calculated using the Mindray hematology analyzer. The results of the study showed that tantalum implants and iron plates have no effect on total leukocytes and differentials leukocytes. The total leukocytes between observations show differences, but are still deep in normal range. The use of tantalum implants and iron plates has no effect against white blood cell images and differential leukocyte percentages.Keywords: leukocytes, differential leukocytes, iron plate, tantalum plate

Pengaruh Crossbreeding Ayam ALPU dan Kamaras terhadap Kadar Protein pada Daging Dada Ayam Alaras; The Effect of Crossbreeding between ALPU and Kamaras Chicken on Protein Content in Alaras Chicken Breast Meat Elsha Amalia Putri; M Aman Yaman; Sugito Sugito; Triva Murtina Lubis; M Jalaluddin; Rastina Rastina
JURNAL ILMIAH MAHASISWA VETERINER Vol 4, No 2 (2020): FEBRUARI-APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v4i2.4527

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh crossbreeding ayam ALPU dan Kamaras terhadap kadarprotein pada daging dada ayam Alaras. Sampel yang digunakan pada penelitian ini adalah daging dada ayam Alaras hasil persilangan ALPU dan Kamaras yang berumur 90 hari sebanyak 12 ekor.Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 kelompok perlakuan dan 3 kali ulangan.Setiap kelompok terdiri atas 3 ekor ayam.Analisakadar protein menggunakan ujiproksimatdengan metode Kjehdahl.Hasil rata-rata (± SD) kadar protein pada daging dada ayam hasil persilangan ALPU (AmAb), Alaras1 (AmKb), Kamaras (KmKb) dan Alaras2 (KmAb) secara berturut-turut yaitu 22.86±0.94 %, 23.99±1.26 %, 23.92±1.74 % dan 22.89±0.83 %. Dari hasil analisa statistik menunjukkan bahwa crossbreeding tidak berpengaruh nyata (P0,05) terhadap kadar protein pada daging dada ayam Alaras.Kata kunci: Alaras, Protein, Daging Dada ABSTRACT The objective of this research was to determine the effect of crossbreeding between ALPU and Kamaras chicken on protein content in Alaras chicken breast meat. Samples were of this research were breast meat from 12 chickens (age 90 days). The research design usedwas a completely randomized design (RAL) with 4 treatments and 3 replications of each. Each treatments consisted 3 chickens.Protein content were analyzed by proximate analysis with Kjehdahl method. The result of protein content in breast meat crossbred chicken ALPU (AmAb), Alaras1 (AmKb), Kamaras (KmKb) and Alaras2 (KmAb) were 22.86±0.94 %; 23.99±1.26; 23.92±1.74 % and 22.89±0.83 % respectively. Based on statistical analysis of this research showed that crossbreeding did not effect significantly (P0.05) on protein content in Alaras chicken breast meat.Keywords: Alaras, Protein, Breast Meat

JUMLAH LEUKOSIT DAN DIFERENSIAL LEUKOSIT GAJAH SUMATERA (Elephas maximus sumatranus) JANTAN BERDASARKAN TINGKATAN UMUR DI PUSAT LATIHAN GAJAH (PLG) MINAS RIAU (Total Leukosit and Differential Leukosit of Male Sumatran Elephant (Elephas maximus sumatranus) Based on Age Level in the Elephant Exercise Center (PLG) Minas, Riau) Atikah Rahma Putri; Triva Murtina Lubis; Arman Sayuti
JURNAL ILMIAH MAHASISWA VETERINER Vol 6, No 2 (2022): FEBRUARI-APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v6i2.8596

Penelitian ini bertujuan untuk mengetahui pengaruh umur terhadap jumlah leukosit total, basofil, eosinofil, neutrofil, limfosit, dan monosit gajah sumatera (Elephas maximus sumatranus) jantan berdasarkan umur di PLG Minas, Riau. Penelitian ini menggunakan sampel darah yang diambil dari 10 ekor gajah sumatera jantan dengan kisaran umur 6-35 tahun. Pengambilan darah dilakukan pada vena auricularis posterior dengan menggunakan vacutainer yang berisi antikoagulan. Jumlah leukosit dan diferensial leukosit ditentukan dengan hematology analyzer Sysmex XN-550. Data yang diperoleh dikelompokkan berdasarkan umur (25 tahun dan 25 tahun) dan dianalisis dengan uji t. Hasil penelitian menunjukkan bahwa rata-rata (±SD) jumlah leukosit total, basofil, eosinofil, neutrofil, limfosit, dan monosit pada umur 25 tahun berturut-turut adalah 14,50±2,34 x103/µl, 0,18±0,08 %, 5,10±2,50 %, 23,56±5,28 %, 36,10±7,99 %, dan 35,06±7,66 % sedangkan pada umur 25 tahun berturut-turut adalah 11,09±3,61 x103/µl, 0,26±0,04 %, 3,74±1,51 %, 22,80±4,74 %, 38,82±12,56 %, dan 34,38±11,61 %. Berdasarkan hasil penelitian dapat disimpulkan bahwa umur tidak berpengaruh (P0,05) terhadap jumlah leukosit total, basofil, eosinofil, neutrofil, limfosit, dan monosit pada gajah sumatera jantan di PLG Minas, Riau. (The aim of this study was to determine the effect of age on total number of leukocytes, basophils, eosinophils, neutrophils, lymphocytes, and monocyte of male sumatran elephants (Elephas maximus sumatranus) base on age in PLG Minas Riau. In the study was used blood samples collected from 10 males sumatran elephants with age range 6-35 years old. Blood was collected is done in the posterior auricular vein using a vacutainer containing anticoagulants. The leukocyte count and leucocyte differentials were determined by the Sysmex XN-550 hematology analyzer. The data obtained were classified by the age (25 years and 25 years) and analyzed by t test. The results showed that the mean (± SD) total leukocyte count, basophils, eosinophils, neutrophils, lymphocytes, and monocytes at age 25 years were 14.50±2.34 x103/μl, 0.18±0,08 %, 5.10±2.50 %, 23.56±5.28 %, 36.10±7.99 %, and 35.06±7.66 % while at age 25 years were 11.09±3.61 x103/μl, 0.26±0.04 %, 3.74±1.51 %, 22.80±4.74 %, 38.82±12.56 %, and 34.38±11.61%. Based on the result, it can be concluded that age has no effect (P0,05) on total leukocyte, basophils, eosinophils, neutrophils, lymphocytes, and monocytes in male sumatran elephants in PLG Minas, Riau.)

Gambaran Histopatologi Ginjal Babi Hutan (Sus scrofa) yang Terinfeksi Parasit Internal di Kawasan Lhoknga, Aceh Besar (HISTOPATHOLOGY OF WILD SWINE (Sus scrofa) KIDNEY INFECTED BY INTERNAL PARASITE IN LHOKNGA REGION, ACEH BESAR) Rhoza Indra; Muttaqien Muttaqien; Ummu Balqis; Winaruddin Winaruddin; Cut Dahlia Iskandar; Triva Murtina Lubis
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 4 (2018): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

ABSTRAKPenelitian ini bertujuan untuk mengetahui gambaran histopatologi ginjal babi hutan (Sus scrofa) yang terinfeksi parasit internal. Sampel penelitian menggunakan 3 ekor babi hutan dengan kriteria terinfeksi parasit internal. Babi hutan (Sus scrofa) di eutanasia dan dilakukan pemeriksaan darah dan feses lalu dinekropsi untuk mengambil ginjalnya. Beberapa bagian dari ginjal diambil untuk diperiksa secara patologi anatomi (PA), setelah itu dibuat sediaan secara histopatologi (HP) dengan menggunakan pewarnaan Haemotoksilin Eosin (HE). Pembuatan sediaan histopatologi diawali dari fiksasi, stopping point, dehidrasi, penjernihan, infiltrasi, penanaman dan pemotongan dengan ketebalan 5 µm. Hasil penelitian menunjukkan bahwa pada pemeriksaan darah babi pertama (B1) anakan jantan terinfeksi Anaplasma marginalis, pemeriksaan PA tidak ada perubahan dan secara HP mengalami nekrosis tubulus, infiltrasi sel radang dalam glomerulus, penyempitan lumen tubulus dan infiltrasi leukosit. Pada pemeriksaan feses babi kedua (B2) anakan jantan ditemukan telur cacing Strongyloides ransomi, pemeriksaan PA tidak ada perubahan dan secara HP mengalami infiltrasi sel radang dalam glomerulus, kapsula bowman mulai menipis, piknotis, kariolisis, karioreksis, dan infiltrasi leukosit. Sementara hasil pemeriksaan pada babi ketiga (B3) anakan jantan tidak terinfeksi parasit namun pada pemeriksaan HP terdapat infiltrasi sel radang.Kata kunci : Babi hutan, Ginjal, Parasit internal, Anaplasma marginalis, Strongyloides ransomi. ABSTRACT This study aims to determine the histopathological picture of wild swine (Sus scrofa) kidney infected with internal parasites. The study sample used 3 wild swine with criteria for infection with internal parasites. Wild swine (Sus scrofa) is euthanized and blood and feces are examined and then decanted to take the kidney. Some parts of the kidneys were taken for anatomical pathology (PA), after which histopathology (HP) was prepared using Haemotoxicillin Eosin (HE) staining. The preparation of histopathological preparations begins with fixation, stopping points, dehydration, purification, infiltration, planting and cutting with a thickness of 5 µm. The results showed that in the first pig blood examination (B1), male tillers were infected with Anaplasma marginalis, PA examination had no change and HP had tubular necrosis, glomerular inflammation in the cell infiltration, narrowing of the tubular lumen and leukocyte infiltration. On the second pig stool examination (B2), male tillers were found on Strongyloides ransomi worm eggs, PA examination had no changes and HP had inflammatory cell infiltration in the glomerulus, the bowman capsule began to thin, picnosis, kariolysis, karioreksis, and leukocyte infiltration. While the results of the examination on the third pig (B3) male tillers were not infected with parasites but on HP examination there was an infiltration of inflammatory cells.Keywords : Wild swine, kindey, internal parasite, Anaplasma marginalis, Strongyloides ransomi

MOTILITAS DAN VIABILITAS SPERMATOZOA SAPI ACEH SETELAH PEMBEKUAN MENGGUNAKAN PENGENCER SITRAT KUNING TELUR DENGAN PENAMBAHAN EKSTRAK KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)(Motility and Viability Spermatozoa Aceh Cattle after Freezing Using Yolk Citrate Diluent with Additional of Peels Red Dragon Fruit Extract (Hylocereus polyrhizus)) melisa lola anggraini; dasrul dasrul; juli melia; triva murtina lubis; rosmaidar rosmaidar; hamdan hamdan
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Penelitian ini bertujuan mengetahui pengaruh penambahan ekstrak kulit buah naga merah (Hylocereus polyrhizus) dalam pengencer sitrat kuning telur terhadap motilitas dan viabilitas spermatozoa sapi aceh setelah pembekuan. Penelitian ini menggunakan semen segar sapi aceh yang dikoleksi menggunakan vagina buatan dan dibagi ke dalam 5 kelompok perlakuan pengencer dengan 5 kali ulangan. Kelompok perlakuan kontrol yaitu semen diencerkan dalam pengencer sitrat kuning telur tanpa penambahan ekstrak kulit buah naga merah (P0), kelompok perlakuan semen diencerkan dalam pengencer sitrat kuning telur ditambah ekstrak kulit buah naga merah 0,2% (P1), 0,4% (P2), 0,6% (P3) dan 0,8% (P4). Data dianalisis menggunakan uji analysis of variance (ANOVA) satu arah dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa penambahan ekstrak kulit buah naga merah konsentrasi 0,0; 0,2; 0,4; 0,6 dan 0,8% dalam pengencer sitrat kuning telur menghasilkan persentase motilitas adalah 23,60 ± 4,72; 26,60 ± 5,94; 36,40 ± 4,98; 45,40 ± 4,56 dan 33,40 ± 10,13. Persentase viabilitas spermatozoa adalah 29,10 ± 5,82; 35,34 ± 5,85; 42,52 ± 4,87; 51,76 ± 7,13 dan 48,6 ± 6,05. Penambahan ekstrak kulit buah naga merah konsentrasi 0,6% menghasilkan persentase motilitas dan viabilitas spermatozoa yang lebih baik dibandingkan konsentrasi perlakuan lainnya. Simpulan penambahan ekstrak kulit buah naga merah dalam pengencer sitrat kuning telur dapat meningkatkan persentase motilitas dan viabilitas spermatozoa sapi aceh setelah pembekuan.(This study aims to determine the effect of addition peels red dragon fruit extract (Hylocereus polyrhizus) in the yolk citrate diluent to the motility and viability of the aceh cattle spermatozoa after freezing. This study used fresh semen of aceh cattle collected using artificial vagina and divided into 5 groups of diluent treatments with 5 replications. The control group of cement was diluted in the yolk citrate dilator without the addition of red dragon fruit skin extract (P0), semen treatment group was diluted in the yolk citrate diluent plus peels red dragon fruit extract 0.2% (P1), 0.4% (P2), 0.6% (P3) and 0.8% (P4). Data were analyzed by using analysis of variance (ANOVA) and continued by Duncan test. The results showed that the addition of peels red dragon fruit extract 0.0; 0.2; 0.4; 0.6 and 0.8% of yolk citrate diluent yields motility percentage is 23.60 ± 4.72; 26.60 ± 5.94; 36.40 ± 4.98; 45.40 ± 4.56 and 33.40 ± 10.13. Percentage of spermatozoa viability were 29.10 ± 5.82; 35.34 ± 5.85; 42.52 ± 4.87; 51.76 ± 7.13 and 48.6 ± 6.05.The addition of peels red dragon fruit extract 0.6% yields better percentage of motility and spermatozoa viability than other treatment doses. The conclusion of addition peels red dragon fruit extract in yolk citrate diluent can increase the percentage motility and viability spermatozoa of aceh cattle after freezing)

Co-Authors Abd. Rasyid Syamsuri Abdul Harris Ahmad Damhoeri Al Azhar Amalia Sutriana Amiruddin . Amiruddin Amiruddin Aris Junaidi Arman Sayuti Atika Agusty Atikah Rahma Putri Awaluddin Awaluddin Baradillah Abdyad Cut Dahlia Iskandar Dasrul Dasrul Dasrul Dasrul Elsha Amalia Putri Elza Savitri Erdiansyah Rahmi Erdiansyah Rahmi Erina Erina Erwin Erwin Fakhrurrazi Fakhrurrazi Gholib Gholib Hamdan Hamdan Hamny Sofyan HEFRI YUNALDI husnurrizal husnurrizal Idawati Nasution Indra Saputra Simbolon Juli Melia M Aman Yaman M Jalaluddin M. Hasan Maiyus Musrianti melisa lola anggraini Muhammad Hambal Muhammad Hambal Muhammad Hasan Muhammad Toras Mulyadi Adam Muslim Akmal Muslim Akmal Muttaqien Muttaqien Nabilah Putroe Agung nikhofebri hidayat Nuzul Asmilia Prabowo Purwono Putro Rahmat Nazif Rastina Rastina Rhoza Indra Rinidar - Rosmaidar Rosmaidar Rusli - Rusli Rusli Sugito Sugito Teuku Armansyah Ummu Balqis Winaruddin Winaruddin Yopi Mardian Yudha Bhaskara Yudha Fahrimal Zaid Zaid Zuhrawati NA Zuhrawaty NA Zuraidawati Zuraidawati

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