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Karakteristik Kalus dari Eksplan Batang Planlet Krisan (Chrysanthemum morifolium Ramat) pada Media dengan Konsentrasi 2,4-Dichlorophenoxyacetic Acid (2,4-D) dan 6-Benzylaminopurine (BAP) serta Kondisi Pencahayaan Setiawati, Tia; Arofah, Annisa Nur; Lestari, Ani; Hasan, Rusdi
Jurnal Pro-Life Vol. 11 No. 3 (2024): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v11i3.6404

Abstract

This research aimed to obtain the optimal concentration combination of 2,4-D and BAP growth regulators for inducing callus from the stem explant of Chrysanthemum (C. morifolium Ramat) in bright and dark conditions. Stem explants of chrysanthemum were grown in culture medium supplemented with various concentrations and combinations of 2,4-D and BAP under different lighting conditions for 45 days after culture. A laboratory-scale experimental method was used in this research using Completely Randomized Design (CRD). The observed parameters were induction time, percentage formation, size, fresh weight, dry weight, texture, color, and various respones generated by callus. All data were analyzed descriptively. The results showed that all concentrations and combinations of growth regulators could induce callus. In bright condition, the fastest callus induction time was 7 days after culture; most calli were dark green and dark brown colored with compact texture; the callus size was 1.36 cm; also, the highest dry weight generated by callus was 0.17 gram. Meanwhile, in dark conditions, the fastest callus induction time was 6 days after culture; most calli were light green and light brown colored with compact texture; the callus size was 1.18 cm; and the highest dry weight generated by callus was 0.15 gram.
Microteaching Lesson Study of Biology Prospective Teacher to Develop Lesson Plan Design and Teaching Ability Lukitasari, Marheny; Hasan, Rusdi; Murtafiah, Wasilatul
Jurnal Pendidikan Indonesia Vol 10 No 3 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.09 KB) | DOI: 10.23887/jpi-undiksha.v10i3.22701

Abstract

This research aimed to develop the learning tools of the biological subject in high school and the teaching biology ability of the teacher candidates through microteaching-lesson study (MLS). The combination of MLS activities was unfolded in preparing the real learning conditions for prospective teachers who will conduct the field practice in the actual classroom. Data were collected through the observation sheets, interviews, and microteaching scores that represented the students' teaching ability. The data were analyzed descriptively. The results showed that each student capable of designing three learning plans with good and excellent categories. The teaching ability and classroom management of students increase by the three activities that conducted, as can be seen from the mean of first (55,77), the second (71,88), and the third (84,38) practice values. However, the students experienced difficulties in the stages of preparation and implementation concerning the more time needed as well as the more cycle activities. Therefore, the students argued that by the only three cycles of activities they experienced, then the LS would be constrained to introduce in the field practice program at school. The more cycles of MLS and guidance from the lecturer is still needed to make prospective teacher ready to implement LS in their field practice at school.
Project-Based Plant Morphology Module as Teaching Material for Students' Concept Mastery Murniati, Neni; Yennita, Yennita; Cahya, Malinda Dwi; Hasan, Rusdi
Biosfer: Jurnal Pendidikan Biologi Vol. 18 No. 1 (2025): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/biosferjpb.49896

Abstract

The necessity for teaching materials that can effectively facilitate students' skills has become increasingly evident in order to enhance concept mastery. The objective of this study is to develop teaching materials in the form of project-based modules on plant morphology for the purpose of facilitating concept mastery. The Project-Based Plant Morphology Module was developed in accordance with the ADDIE model (Branch, 2009), which comprises five phases: analysis, design, development, implementation, and evaluation. The research instruments utilized for the development process included a validation questionnaire, a student response questionnaire, and a concept mastery test. Validity of the module was obtained through the use of instruments in the form of book validation sheets, which were completed by experts in the fields of media, materials, and education. The module trials were obtained through the implementation instruments for the individual components and the management of the learning implementation. Product trials will be conducted with students enrolled in the Biology Education program during the second semester of the 2022/2023 academic year. The data used to validate the module was obtained from the module's expert validators using a validation sheet. The data obtained from the product trial is analyzed in order to assess the readability of the module. The results indicated that the plant morphology module was classified as "very feasible." The responses of students to the module indicated that it was categorized as "very easy to understand". Consequently, the plant morphology module is deemed a valuable and effective component of plant morphology courses
Perbedaan Struktur Morfologi, Anatomi, dan Fisiologi serta Kandungan Fitokimia Daun Aur-Aur (Commelina diffusa) pada Intensitas Cahaya Berbeda di Embung Leuwi Padjadjaran Handayani, Seviana Esti; Hasan, Rusdi; Mutaqin, Asep Zainal
Pro-Life Vol. 12 No. 1 (2025): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Commelina diffusa (family Commelinaceae) contains various secondary metabolites with potential antimicrobial and antioxidant properties. The production of these compounds is influenced by environmental factors, exceptionally light intensity, which also plays a role in determining the plant’s morphological and anatomical characteristics. This study aims to analyze differences in leaf morphology, stomatal anatomical structure, chlorophyll content, and phytochemical compounds in C. diffusa leaves growing in two locations with different light conditions at Embung Leuwi Padjadjaran, Sumedang. A descriptive method was used to examine leaf morphology, stomatal anatomy, chlorophyll content, and phytochemical compounds of C. diffusa in shaded and unshaded locations. The results showed that leaf morphology was relatively similar in both locations, but the leaf surface area was more significant in unshaded conditions (8,33 cm²). The stomata were hexacytic type with higher stomatal density in the shaded condition (591,082 cell/mm²) and a higher chlorophyll content (37,73 CCI). The result of Phytochemical analysis confirmed the presence of alkaloids, flavonoids, saponins, tannins, and quinones, which were relatively higher in unshaded conditions.
THE ABILITY OF WATER HYACINTH (Eichhornia crassipes) AS A BIOACCUMULATOR OF HEAVY METALS IN WASTEWATER FROM THE RUBBER PROCESSING INDUSTRY Hasan, Rusdi; Setiawati, Tia; Nopriyeni, Nopriyeni
JURNAL BIOEDUKASI Vol 8, No 1: Jurnal Bioedukasi Edisi April 2025
Publisher : UNIVERSITAS KHAIRUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/bioedu.v8i1.9808

Abstract

This study aimed to assess the ability of water hyacinth (Eichhornia crassipes) as a bioaccumulator of heavy metals copper (Cu) and zinc (Zn) in rubber industry wastewater at PT Batanghari, Bengkulu. The research methods included water and water hyacinth sampling from effluent ponds and controls, followed by analysis of Cu and Zn levels using atomic absorption spectrophotometry (AAS). Results showed that Zn levels in wastewater reached 143.0884 mg/l (28 times the quality standard), while Cu was 0.1845 mg/l (still below the quality standard). Water hyacinth accumulated metals in different patterns: Cu was highest in the roots (1.4480 mg/l), while Zn was evenly distributed in the roots (6.7261 mg/l) and leaves (6.6473 mg/l). Analysis of bioaccumulation (BAF) and translocation factors (TF) revealed the efficiency of water hyacinth in absorbing metals, especially Zn, although the absorption mechanism is selective and influenced by environmental conditions such as pH, dissolved oxygen, and the presence of organic compounds. The implications of this study emphasize the potential of water hyacinth as an economical and sustainable phytoremediation solution for the rubber industry, with recommendations of implementing a constructed wetland system and periodic harvesting for optimization.
Uji Antimikroba Minyak dengan Variasi Katalisator Hasil Pengolahan Limbah Medis Padat Pasca Sterilisasi Secara Pirolisis terhadap Bakteri Staphylococcus aureus Alam, Vania Aramintana; Hasan, Rusdi; Rosada, Keukeu Kaniawati; Fitria, Novi
JURNAL BIOEDUKASI Vol 7, No 2: Jurnal Bioedukasi Edisi Oktober 2024
Publisher : UNIVERSITAS KHAIRUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/bioedu.v7i2.8365

Abstract

Semakin hari peningkatan limbah medis semakin bertambah seiring dengan pertumbuhan ekonomi. Pengolahan limbah medis dapat dilakukan dengan metode insinerasi, microwaving, hingga pirolisis. Dalam proses pirolisis diperlukan katalisator untuk mempercepat reaksinya. Hasil pirolisis dapat berupa cair, padat, dan gas. Tujuan dari penelitian ini adalah menganalisis perbedaan uji resistensi antimikroba dari minyak kontrol dan dengan variasi katalisator hasil pengolahan limbah medis padat pasca sterilisasi dengan metode pirolisis menggunakan bakteri Staphylococcus aureus yang tidak dapat mendegradasi hidrokarbon. Minyak hasil pirolisis limbah medis padat kemudian di uji antimikroba dengan bakteri S. aureus dengan metode sumuran. Metode yang digunakan dengan mengukur zona bening yang terbentuk pada media lalu dianalisis dengan uji anova satu arah. Hasil analisis menunjukkan bahwa rata-rata zona bening yang terbentuk dari minyak kontrol, etilen glikol, dan N-Heksana adalah 15,88 mm, 15,06 mm, dan 13,56 mm dimana termasuk ke dalam kategori kuat. Hal ini menunjukkan bahwa sampel minyak hasil pirolisis memiliki aktivitas sensitivitas kuat terhadap bakteri S. aureus dan bakteri tidak mendegradasi senyawa hidrokarbon. Hasil analisis statistik menunjukkan nilai 0,247 yang menunjukkan bahwa tidak terdapat perbedaan secara signifikan antara minyak kontrol, minyak etilen glikol, dan minyak N-Heksana terhadap zona bening yang terbentuk dengan bakteri uji.    Kata kunci: Antimikroba, limbah medis padat, pirolisis, Staphylococcus aureus
The Growth of Oyster Mushroom on Ramie Chip Waste-Derived Media was Accelerated by Rice-washed Water Budiono, Ruly; Washfanisa, Handina Alya; Mutaqin, Asep Zainal; Kusmoro, Joko; Nurzaman, Mohamad; Setiawati, Tia; Hasan, Rusdi
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33433

Abstract

Rice-washed water is wastewater resulted from the rice cleaning process that is rich in nutrients. The use of ramie chip waste can be considered a potential substrate due to its lignocellulosic nature, similar to other wastes studied. This study aimed to examine the effect of rice-washed water on the mycelium and pinhead growth of white oyster mushrooms grown in the ramie chip waste as the growing medium. The experiment was conducted for three months using a completely randomized design with four rice-washed water treatments and six replications. The rice-washed water concentration treatments were 0% (K0), 25% (K1), 50% (K2), and 100% (K3), which were added to the ramie chip waste as the growing medium for white oyster mushroom. The time of mycelium propagation velocity, the time the first pinhead appearance, the number of mushroom bodies, and the largest cap diameter were observed. The results showed that ramie chip waste-derived media was suitable to cultivate white oyster mushrooms. Adding 100% rice-washed water to the media significantly affected the speed of mycelium propagation (16.51 days) compared to control (30 days) and the appearance of the first pinhead (31.5 days) compared to control (38.5 days) as well as the number of fruiting bodies of mushroom (8.8) compared to control (6.2). However, the rice-washed water application did not significantly affect the diameter of the largest mushroom cap.
The Implementation of Scaffolding in Team Games Tournament (TGT) to Improve Students’ Participation and Learning Performance on Reproduction System Topics Arindasandy, Devi; Rahmawati, Rita Nur; Hasan, Rusdi; Lukitasari, Marheny
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11923

Abstract

Background: This study aims to describe the usage of scaffolding methods in the activeness and learning performance of students with Teams Games Tournament (TGT) learning at SMA Negeri 5 Madiun. The type of analysis used is class action research (PTK). The subjects of this study are students of class XI MIPA 6 at SMA Negeri 5 Madiun. Methods: The method of data collection uses observation sheets to understand students’ activeness and pre-tests and post-tests to assess students’ learning performance. The technique used for data analysis is descriptive-quantitative. Results: The results of students’ activeness in Cycle I and Cycle II are 94% and 95%, respectively. There is also an improvement in students’ learning performance, as seen from the pre-tests and post-test results. The post-test results in Cycle I and Cycle II are 86% and 97%, respectively. The data analysis shows that applying the scaffolding method in Teams Games Tournament (TGT) learning can improve students’ activeness and learning performance in class XI MIPA 6 at SMA Negeri 5 Madiun. Conclusions: Applying the scaffolding learning method to team games and tournament learning can increase the activeness and performance of students in the reproductive system material.
Local Knowledge of The Community in Mekarwangi Village on Long-Tailed Macaque (Macaca fascicularis) Rehansha, Canaya Vania; Hasan, Rusdi
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 1: March 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i1.23855

Abstract

Local knowlede is a phenomenon that is felt and experienced by the people of a particular area who have local understanding regarding the natural resources found in the surrounding environment. One of this knowledge is about long-tailed macaque (Macaca fascicularis). This research aims to examine local knowledge of the community regarding long-tailed macaque in Mekarwangi Village which borders Taman Hutan Raya Ir. H. Djuanda which is one of the long-tailed macaque conservation sites. The method used in this research is a qualitative approach through observation, semi-structured interviews, and strengthened by literature study. The result obtained were analyzed descriptively. The reseach result show that the community knows about the existence of long-tailed macaque. Community interactions with long-tailed macaque pose a threat to long-tailed macaque, some individu of long-tailed macaque be arrasted because people are disturbed by their existance. The majority of community in Mekarwangi Viilage know the food, habitat, and daily activities of long-tailed macaque from daily observation and some knowledge passed down from previous generations.