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Journal : Pro-Life

Aplikasi Asam Humat dan Pupuk Organik Cair Ekstrak Rumput Laut untuk Meningkatkan Pertumbuhan dan Kandungan Flavonoid Brassica juncea L. Setiawati, Tia; Fitria, Sania Septiani; Nurzaman, Mohamad; Hasan, Rusdi; Mutaqin, Asep Zainal; Lestari, Ani
Jurnal Pro-Life Vol. 11 No. 2 (2024): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This research was conducted with the aim of obtaining the optimal dose of humic acid (HA) and liquid organic fertilizer (LOF) from seaweed extract that can enhance the growth and total flavonoid content of Brassica juncea L. The method used in this study was experimental with a randomized complete block design with two factors. The first factor was HA, consisting of 4 dose levels: 0, 4, 8, and 12 g/kg, while the second factor was LOF from seaweed extract, consisting of 4 dose levels: 0, 1, 2, and 3 mL/L. Each treatment was replicated four times. Parameters were observed on the 42nd day, including plant height, leaf number, leaf area, dry weight, and total flavonoids. Data were analyzed using Analysis of Variance (ANOVA) and followed by Duncan's Multiple Range Test with a confidence level of 95%. The results showed that the dose of 4 g/kg HA and 2 mL/L LOF was the best dose for enhancing growth and total flavonoid content, producing an average plant height of 19.25 cm, a leaf number of 5.75, a total leaf area of 179.8 cm2, a dry weight of 0.57 grams, and a total flavonoid content of 0.106 mg QE/g sample.
Karakteristik Kalus dari Eksplan Batang Planlet Krisan (Chrysanthemum morifolium Ramat) pada Media dengan Konsentrasi 2,4-Dichlorophenoxyacetic Acid (2,4-D) dan 6-Benzylaminopurine (BAP) serta Kondisi Pencahayaan Setiawati, Tia; Arofah, Annisa Nur; Lestari, Ani; Hasan, Rusdi
Jurnal Pro-Life Vol. 11 No. 3 (2024): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/pro-life.v11i3.6404

Abstract

This research aimed to obtain the optimal concentration combination of 2,4-D and BAP growth regulators for inducing callus from the stem explant of Chrysanthemum (C. morifolium Ramat) in bright and dark conditions. Stem explants of chrysanthemum were grown in culture medium supplemented with various concentrations and combinations of 2,4-D and BAP under different lighting conditions for 45 days after culture. A laboratory-scale experimental method was used in this research using Completely Randomized Design (CRD). The observed parameters were induction time, percentage formation, size, fresh weight, dry weight, texture, color, and various respones generated by callus. All data were analyzed descriptively. The results showed that all concentrations and combinations of growth regulators could induce callus. In bright condition, the fastest callus induction time was 7 days after culture; most calli were dark green and dark brown colored with compact texture; the callus size was 1.36 cm; also, the highest dry weight generated by callus was 0.17 gram. Meanwhile, in dark conditions, the fastest callus induction time was 6 days after culture; most calli were light green and light brown colored with compact texture; the callus size was 1.18 cm; and the highest dry weight generated by callus was 0.15 gram.
Perbedaan Struktur Morfologi, Anatomi, dan Fisiologi serta Kandungan Fitokimia Daun Aur-Aur (Commelina diffusa) pada Intensitas Cahaya Berbeda di Embung Leuwi Padjadjaran Handayani, Seviana Esti; Hasan, Rusdi; Mutaqin, Asep Zainal
Pro-Life Vol. 12 No. 1 (2025): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Commelina diffusa (family Commelinaceae) contains various secondary metabolites with potential antimicrobial and antioxidant properties. The production of these compounds is influenced by environmental factors, exceptionally light intensity, which also plays a role in determining the plant’s morphological and anatomical characteristics. This study aims to analyze differences in leaf morphology, stomatal anatomical structure, chlorophyll content, and phytochemical compounds in C. diffusa leaves growing in two locations with different light conditions at Embung Leuwi Padjadjaran, Sumedang. A descriptive method was used to examine leaf morphology, stomatal anatomy, chlorophyll content, and phytochemical compounds of C. diffusa in shaded and unshaded locations. The results showed that leaf morphology was relatively similar in both locations, but the leaf surface area was more significant in unshaded conditions (8,33 cm²). The stomata were hexacytic type with higher stomatal density in the shaded condition (591,082 cell/mm²) and a higher chlorophyll content (37,73 CCI). The result of Phytochemical analysis confirmed the presence of alkaloids, flavonoids, saponins, tannins, and quinones, which were relatively higher in unshaded conditions.