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Effect of Phosphate-based Glass Porous Microspheres (P30) Loaded with Extracellular Vesicle on Osteoblast Behaviour: In Vitro Study Cahayadi, Sigit Daru; Nuzulia, Nur Aisyah; Boediono, Arief; Ahmed, Ifty; Nurhayati, Retno Wahyu; Sari, Yessie Widya; Juliandi, Berry
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.840-849

Abstract

Bioactive materials, particularly phosphate-based glasses (PBG), hold great promise in bone repair due to their controllable degradation rates and bioactivity. This study evaluated PBG porous microspheres (P30) loaded with extracellular vesicles (EVs) for bone tissue engineering, focusing on osteogenesis, EV uptake, and cell invasion. P30 concentrations (5P30, 10P30, 50P30, 100P30, 500P30) were tested for their effects on calcification, EV uptake, and cell migration. Results showed that 100P30 exhibited optimal conditions for osteogenesis and EV delivery, with the highest calcification areas at both Day 7 and Day 14 and the most efficient EV internalization. Meanwhile, 500P30 demonstrated the highest cell migration, supporting pre-osteoblastic migration at this concentration. These findings indicate that 100P30 is ideal for mineralization and EV uptake, while 500P30 enhances cell invasion. This study highlights P30's versatility as a biomaterial for bone regeneration, with specific concentrations tailored to different regenerative goals. These results underscore the potential of P30 microspheres loaded with EVs as an effective strategy for promoting bone repair and regeneration.
EGGSHELL CALCIUM NANOPARTICLES: A SUSTAINABLE APPROACH TO BOOST BIOGAS PRODUCTION FROM TOFU LIQUID WASTE Sari, Yessie Widya; Attar, Zia Rausyanfikr; Widyarani, Widyarani
Indonesian Physical Review Vol. 7 No. 3 (2024)
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/ipr.v7i3.350

Abstract

This study explored enhancing biogas and methane production from tofu liquid wastewater (TLW) by adding calcium nanoparticles. Eggshell calcium nanoparticles (ECN) in the form of nano Ca (OH)2 were introduced to improve the degradation process. Chicken eggshells were calcined at 1000 °C to create Ca (OH)2 particles, followed by milling to yield ECN. Characterization using X-ray diffraction (XRD) confirmed the presence of Ca (OH)2 in the ECN while scanning electron microscopy (SEM) revealed the irregular morphology of the particles. Energy-dispersive X-ray spectroscopy (EDS) analysis showed calcium and oxygen as the primary elements. To investigate the effect of ECN in enhancing biogas and methane production, we evaluated 3 levels of ECN concentrations during anaerobic fermentation of TLW: 2.5 g/L, 5 g/L. and 7.5 g/L. We observed that adding ECN of 5 g/L during anaerobic digestion improved biogas production. Further, at this concentration, the methane concentration on the biogas was 64%, while on the control samples (without ECN) was only 0.09%. These findings suggested the benefit of ECN supplementation during anaerobic digestion of TLW for biogas production.    
EDIBLE SPRAY COATING WITH WATER HYACINTH CELLULOSE AND COCONUT HUSK-BASED CARBON BLACK FOR FOOD APPLICATIONS Azahra, Annisa Nur; Akmal, Raihan Muhammad; Nabila, Pristy Tasya; Bayah, Salsabilla Permata; Fauji, Muhamad Iqbal; Sari, Yessie Widya
Indonesian Physical Review Vol. 8 No. 1 (2025)
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/ipr.v8i1.390

Abstract

Food waste and microbial contamination have led to an annual increase in foodborne diseases. One potential solution is the application of an edible spray coating (ESC) as a bioplastic, incorporating antibacterial agents. The spray technique is considered most effective due to its ease of application and controllable thickness. This research evaluates carbon black (CB) derived from coconut husk waste as an antibacterial agent in ESC. Coconut husk was selected because it contains up to 54% lignin. The ESC was formulated using a mixture of hydroxypropyl methylcellulose (HPMC), k-carrageenan, water hyacinth cellulose, and glycerol. Additionally, other antibacterial agents such as silver and chitosan were included as positive controls. The CB was produced through pyrolysis of coconut husk at 700 °C, with nitrogen gas added at a heating rate of 5 °C/min. The water hyacinth cellulose was extracted using a chemical method. In this study, ten ESC formulations were tested with varying concentrations of silver, chitosan, and CB. The resulting ESC viscosities ranged from 16.8 to 46.9 cP. Antimicrobial activity against E. coli and S. aureus demonstrated that ESC with 1.5% w/v CB exhibited antibacterial activity with inhibition zones of (2.2±0.3) mm and (32±3) mm, respectively. The application of ESC on cherry tomatoes and strawberries showed that samples containing CB experienced a lower weight loss over time. This indicates the potential of CB in preventing microbial contamination.
ANALISIS EKSERGI KIMIA SISTEM KONVERSI LIMBAH PADAT CANGKANG SAWIT DENGAN TEKNIK PIROLISIS PLASMA Purawiardi , Ibrahim; Aribah, Putri; Aminuddin, Aminuddin; Dimyati , Arbi; Sari, Yessie Widya; Elbersen, Wolter; Maddu, Akhiruddin
Jurnal Rekayasa Mesin Vol. 16 No. 1 (2025)
Publisher : Jurusan Teknik Mesin, Fakultas Teknik, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/jrm.v16i1.1847

Abstract

Chemical exergy analysis was commonly used to evaluate the effectiveness of some energy conversions derived from biomass. In this study, this analysis was conducted to evaluate oil palm kernel shell conversion via high-temperature plasma treatment. The plasma treatment was conducted with a DC power source with a voltage of 12 V and current variations of 40, 50, 60, 70, and 80 A. This chemical exergy efficiency was calculated based on gas chromatography (GC) analytical results. The calculated results showed that the chemical exergy efficiency increased exponentially as the current increased. Thus, based on this result, the arc plasma treatment is more recommended to be applied for the pyrolysis application of oil palm kernel shell than its gasification.
Correlation between Time Variation of Steam Distillation of Temu Hitam’s Rhizomes (Curcuma aeruginosa) and Antibacterial Activity of Essential Oil Munawaroh, Fauziyatul; Luthfiana, Widya; Suparto, Irma Herawati; Sari, Yessie Widya; Wahyuni, Wulan Tri; Batubara, Irmanida
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.345

Abstract

Distillation time can affect the extraction of essential oils such as the rhizome of temu hitam (Curcuma aeruginosa) which has been shown to have antibacterial activity. Therefore, this study aims to determine the optimum distillation time to produce temu hitam rhizome oil with the strongest antibacterial activity. The rhizomes of temu hitam were steam distilled with different length of time every 30 minutes for 4 hours to obtain essential oil and determined the compounds contained in them using gas chromatography-mass spectrometry. Antibacterial activity was determined by disc diffusion against Streptococcus mutans, Escherichia coli, and Staphylococcus aureus. The most dominant compounds present in essential oils are eucalyptol (34.89%), epicurzerenone (20.58%), and camphor (16.90%) based on the average peak of the total distillation time. The strongest antibacterial potency was obtained in the first 30 minutes of distillation for S. mutans and E. coli, and in the seventh 30 minutes of distillation for S. aureus.
Effect of Precursor Solvent on the Carbon Micro-Structures Derived from Spray Pyrolysis of Pine Resin (Gondorukem): Preliminary Study Jayadi; Widayatno, Wahyu Bambang; Wismogroho, Agus Sukarto; Firdharini, Cherly; Maddu, Akhiruddin; Alatas, Husin; Sari, Yessie Widya
Jurnal Sains Materi Indonesia Vol. 25 No. 2 (2024): Jurnal Sains dan Materi Indonesia
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jsmi.2024.893

Abstract

Carbon materials have been widely used in various fields. This study aimed to produce carbon using spray pyrolysis with pine resin (gondorukem) as the precursor and different solvents, namely gondorukem-acetone (GAC), gondorukem-ethyl acetate (GEA), and gondorukem-dichloromethane (GDC). The precursor was prepared in a 1:8 (m/v) ratio, and the spray pyrolysis method was employed by heating the atomized precursor solution in the heating zone of a tube furnace. The atomization precursor was infused with nitrogen gas at a rate of 1 l/min with furnace temperature set at 1000°C with heating times of 5, 10, and 20 mins. The carbonaceous materials produced from the pyrolysis were collected on the wire mesh 1000 that was put on a stainless pipe. Carbon that has been coated on the wire mesh 1000 was analyzed using the optical microscope (OM). The physical properties and morphology of the synthesized carbonaceous material were analyzed using field emission scanning electron microscopy (FE-SEM), Fourier-transform infrared spectroscopy (FTIR), Raman, and Brunaur-Emmett-Teller (BET). Based on FE-SEM analysis, the particle size of the GAC sample has an average of 283.58 nm and the highest carbon content, which reached an average of 97.312 At%. GAC samples had the lowest disorder properties in the Raman spectroscopy test, with the value of ID/IG reaching 0.795764. The functional groups observed were C–H stretching at 2920.49 cm-1, N–H bending at 1629.07 cm-1, and C–O stretching at 1159.70 cm-1. Based on carbon content, disorder properties, and functional group stabilization, carbon from the GAC precursor provides the ideal characteristics to be used as a filter material in medical masks. Meanwhile, based on BET testing, the carbon materials from GEA have the ideal material morphological properties to be used as a filter in medical masks. Spray pyrolysis is an efficient method for producing carbon materials, and the use of gondorukem as the precursor shows great potential for various applications.
Bone growth evaluation in collagen-hydroxyapatite implant locations using digital radiography: an animal model Sari, Laela; Julia, Siti; Lubis, Lukmanda Evan; Sihono, Dwi Seno Kuncoro; Sari, Yessie Widya; Soejoko, Djarwani Soeharso
Medical Journal of Indonesia Vol. 32 No. 4 (2023): December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.237051

Abstract

BACKGROUND Digital radiography has been used to evaluate the progress of bone growth with a collagen-hydroxyapatite implant in rabbit tibias. This study aimed to introduce digital radiography methods that provide comprehensive data availability for continuous information retrieval from the implant preparation to the cultivation period. METHODS 38 digital radiographs were divided into 3 treatment groups, namely a single defect without implant (control), single-implant, and three-implant. Radiographic acquisitions were performed at preparation time and post-implantation from 0 to 56 days. Observations were concentrated on the implantation site, followed by creating a lateral profile. The prediction of implantation growth was determined using relative bone density (RBD) percentage. RESULTS Based on the profile, the recovery process consisted of implant absorption and new bone tissue deposition. The absorption process was highly influenced by the defect size. In the control and single-implant groups, regardless of the different recovery processes, similar recovery results were observed 56 days post-implantation, with an RBD value of approximately 90%. Meanwhile, the three-implant group only had an RBD value of 62%. CONCLUSIONS Radiography can evaluate absorption and new bone growth during implantation in New Zealand white rabbits. Radiographs, which can be obtained at any time during cultivation, offered more information on the recovery implantation process than the other method that relies on data obtained after sacrificing the animals.