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ERNIN HIDAYATI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Social Sciences Other

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Isolation of Entomopathogenic Lysinibacillus sphaericus from Sewage at Some Housing Complex in Mataram City and Evaluation of Its Toxicity Against Aedes aegypti Larvae in Laboratory Dewi, Novia Devi Kusuma; Hidayati, Ernin; Sarkono, Sarkono; Prasedya, Eka Sunarwidhi; Suryadi, Bambang Fajar
Biotropika: Journal of Tropical Biology Vol. 10 No. 1 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.01.07

Abstract

The aims of this study were to isolate Lysinibacillus sphaericus from sewage at some housing complex in Mataram city and evaluate its toxicity against third Aedes aegypti larvae. The bacteria collected from mud taken from diluted sewage and objected to heat shock procedure at 80ºC for 30 minutes. The microbiological culture was done using a Nutrient Agar solid medium and incubated at 30ºC for 72 hours. Bacterial characterization was done based on bacterial colony morphology, cell morphology, cell physiology, and cell biochemistry characteristics. Toxicity test on 3rd Aedes aegypti larvae was done for 24, 48, and 72 hours applying Nutrient Broth medium with various dilution. From this study total of 11 isolates of Lysinibacillus sphaericus were isolated, only three isolates showed a high killing rate against Ae. aegypti in 24-hour observation. They were isolate Bs9-2-3 (LC50 24-hour value was 1.75 x 104 cell/mL), isolate Bs9-1-5 (LC50 24-hour value was 6.23 x 104 cell/mL) and isolate Bs2-1-2 (LC50 24-hour value was 7.17 x 106 cell/mL). These local isolates of L. sphaericus had good potential to be developed for bacterial-based biopesticide/biolarvicide for battling Aedes mosquito larvae in the near future.
Co-Authors Adhawati, Laela AIDA MUSPIAH Ambarawati, Dewi Antonius Suwanto Aprianingsih, Yanti Aris Tri Wahyudi Dewi, Novia Devi Kusuma Diniati, Herild fatur Rahman, fatur Faturrahman Hamidia, Baiq Zulifa Hemidia, Baiq Zulifa Ibadurahman, Lalu Fakih Ibadurahman, Lalu Faqih Ito, Michiho Julisaniah, Nur Indah Kurniasih Sukenti Kusuma, A. A. Ngurah Nara Kusuma, Anak Agung Nara Liani, Iin Made Raningsih Mamika Ujianita Romdhini, Mamika Ujianita Muhammad Sarkono Nadia, Ahsanu Pradita, I Made Nanda Prasedya, Eka Sunarwidhi Prazali, Arianteza RAHAYU WIDYASTUTI Sadjidah, Zazhara Aulia Setiawan, Moh. Andi Sidhiawan, Anak Agung Putu Sukiman Sukiman Sukiman Sunarwidhi , Anggit Listyacahyani Sunarwidhi, Anggit Listyacahyani Supiatin Suripto Suripto Suryadi, Bambang Fajar Takamatsu, Sakura Tri Mulyaningsih Yanagisawa, Masayuki Yuhana, ⁠Erna Yumi, Fujiwara