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PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION Ihsan, Bagus Muhammad; Sucipto, Cecep Dani; Khayan, Khayan
Journal of Vocational Health Studies Vol. 5 No. 1 (2021): July 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V5.I1.2021.26-30

Abstract

Background: Filariasis patients can be a source of transmission if their blood still contains microfilariae. One of the Polymerase Chain Reaction (PCR) methods used is High Resolution Melting (HRM), using primary specificity testing. Purpose: To test the specificity of SLX primer. The samples used for this test were isolates of Salmonella., Klebsiella, Pseudomonas, negative and positive controls for Brugia malayi and Wuchereria bancrofti. Method: The design in this study is a quasi-experiment by testing the specificity of SLX primer using HRMbased real-time PCR based on the Cycle Threshold (CT) value observed through the amplification curve. Result: The real-time PCR results showed that no CT was released in the bacterial samples, and there was a CT value in the positive control. The results of this study indicate that specific SLX primer can be used in identifying microfilariae. Conclusion: SLX primer have a reasonable specificity because they cannot detect the existence of microorganisms in the samples other than microfilariae.
IDENTIFICATION OF MICROFILARIAE USING CONVENTIONAL POLYMERASE CHAIN REACTION AND QPCR-HRM Ihsan, Bagus Muhammad; Lakshmi Puspita, Widyana; Triana, Linda; Wahdaniah; Khayan; Dani Sucipto, Cecep
Journal of Vocational Health Studies Vol. 8 No. 1 (2024): July 2024 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V8.I1.2024.42-47

Abstract

Background: The presence of filarial worms in the lymph nodes can result in acute symptoms, such as inflammation of the lymph nodes and ducts, particularly in the groin region. As part of the life cycle of filariasis, symptomatic or asymptomatic patients with microfilariae in their blood can transmit the disease via mosquito bites. The inspection of microfilariae that is currently being developed uses Polymerase Chain Reaction (PCR) to carry out a unique DNA search technique. Purpose: Identify the type of microfilaria present in filariasis patients using Quantitative PCR High- Resolution Melting (qPCR-HRM) and conventional PCR techniques. Method: This study involved the examination of 19 samples using the qPCR-HRM method. Subsequently, the results that were considered positive for microfilaria underwent further testing using conventional PCR. Result: The results of the examination using these two methods revealed the presence of Brugia malayi and Wuchereria bancrofti microfilariae with peak melting temperatures ranging from 78.2 – 78.7 °C and 80.8 – 81.2 °C, and fragment sizes of 199 bp and 227 bp, respectively. Conclusion: Based on the results of the identification from these two methods, it is evident that microfilariae of Brugia malayi and Wuchereria bancrofti can be detected using both conventional and qPCR-HRM methods.
Evaluation of Manganese (Mn) and Cadmium (Cd) Heavy Metal Content in Staple Foods Based on Food Sources and Environmental Conditions in Pregnant Women in Sambas Regency, Indonesia Taufik Anwar; Slamet; Supriyanto; Cecep Dani Sucipto; Suharno
Gema Lingkungan Kesehatan Vol. 23 No. 2 (2025): Gema Lingkungan Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/gelinkes.v23i2.268

Abstract

Exposure to heavy metals, particularly Manganese (Mn) and Cadmium (Cd), in staple foods is a serious health issue for pregnant women in Sambas Regency, Indonesia. This study aims to evaluate the content of Mn and Cd in staple foods and environmental factors that influence exposure in pregnant women. The method used was an analytical observational design with a cross-sectional approach, involving 93 pregnant women selected by purposive sampling. Data were collected through structured interviews and laboratory analysis using atomic absorption spectrometry. The results showed that the average level of Mn in rice was 7.37 mg/kg, while the average level of Cd was 0.056 mg/kg. A total of 68.8% of respondents obtained their rice from local markets, potentially increasing the risk of heavy metal exposure. In addition, 87.1% of respondents reported using house paint, which could be an additional source of exposure. Conclusions from this study emphasise the need for closer monitoring of food quality and education on the dangers of heavy metal exposure for pregnant women, as well as the need for further research to understand the cumulative impact of multi-metal exposure in the context of pregnancy.
Effect of Cleaning Process on Physical and Microbiological Air Quality in Hospital Environment: Case Study of Bhakti Dharma Husada Hospital Febrianna, Fatima Intan; Rokhmalia, Fitri; Suryono, Hadi; Sulistio, Irwan; Sari, Ernita; Sucipto, Cecep Dani; Imami, Ahmad Daudsyah
Jurnal Higiene Sanitasi Vol. 5 No. 1 (2025)
Publisher : Jurusan Kesehatan Lingkungan Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/hisan.v5i1.101

Abstract

Examination of air quality at Bhakti Dharma Husada Surabaya Hospital showed a non-conformity of standards in the LAF Room (Laminair air flow). The study aimed to analyse the physical and microbiological air quality factors in the cleaning process in the LAF (Laminair Air Flow) room. The study used an observational descriptive design with a cross-sectional approach. The study population was room air in the LAF room. The research variables included temperature, humidity, lighting, air germ count, Staphylococcus, and room cleaning process. Data collection techniques were observation, measurement, and laboratory examination. Data were analysed and presented descriptively. The study's results showed the value of the air germ number before the cleaning process was 16 CFU/m3, exceeding the required quality standard of 10 CFU/m³. Measurements after the cleaning process have met the requirements of 9 CFU/m³. Room temperature and humidity measurements before and after the cleaning process have met the quality standards of 16-25°C and 35-50%. The lighting measurement of 110.6 lux does not meet the minimum requirement of 500 lux. Environmental health officers should monitor room cleaners for compliance with the use of personal protective equipment. It is necessary to increase the intensity or modify the lighting.
Aplikasi teknologi tepat guna pengolahan air sebagai sumber air bersih masyarakat Khayan; Ihsan, Bagus Muhammad; Sucipto, Cecep Dani; Puspita, Widyana Lakshmi
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 4 No. 2 (2023)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v4i2.20460

Abstract

Gangguan kesehatan di Indonesia yang berhubungan dengan penyakit yang ditularkan melalui air (Water Borne Disease) masih tergolong tinggi, seperti Diare dan hepatitis. Timbulnya penyakit menular terkait Water Borne Disease tersebut, diantaranya karena tidak terpenuhinya kuantitas dan kualitas air baku sebagai sumber air minum yang memenuhi syarat kesehatan. Metode pengabdian masyarakat ini dilakukan beberapa metode, yaitu pendekatan masyarakat, penyuluhan dan pembuatan sarana Pengolahan Air Permukaan dengan Teknologi Tepat Guna (TTG) kombinasi, yaitu dengan Aerasi, Filtrasi Pasir dan Absorpsi Kabon Aktif untuk Menurunkan kandungan Fe, Kekeruhan, Warna dan pH Air permukaan sebagai sumber air minum masyarakat di Desa Pranan Kota Serang. Hasil dan kesimpulan dari pengabdian pada masyarakat ini adalah Terdapat peningkatan pengetahuan masyarakat tentang TTG Filtrasi Air Bersih , Terciptanya revitalisasi sumber air bersih, transfer teknologi serta dampak sosial bagi masyarakat yaitu perubahan pola kegiatan mandi dan mencuci yang sebelumnya dilakukan di kali pembuangan air sawah setelah pelaksanaan pengabdian kegiatan tersebut beralih ke rumah masing-masing dan Metode penjernihan air secara filtrasi terbukti dapat meningkatkan kualitas air sumur bor ,sehingga air berada pada ambang batas layak digunakan untuk kehidupan sehari-hari. Dengan ini masyarakat setempat tidak lagi mengambil air dari sungai pembuangan air sawah.
PENDAYAGUNAAN LUMPUR GAMBUT DAN KOTORAN SAPI UNTUK MEMPERCEPAT COMPOSTING PADA SAMPAH RUMAH TANGGA Ihsan, Bagus Muhammad; Khayan, Khayan; Sucipto, Cecep Dani; Puspita, Widyana Lakshmi; Nasihin, Nasihin
Jurnal Abdi Insani Vol 11 No 1 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i1.1425

Abstract

Environmental pollution-based diseases, nationally like ISPA, malnutrition, diarrhea and thypus are still high. These diseases can be transmitted through disposal of local households and farms that are naturally disposed of (traditionally) unhealthy, such as open dumping, irrigation and dumping in wells. (parit). To control parasites such as helminths and their vectors, health technology is needed, especially for the use of domestic garbage and local cattle debris feces for composting. In addition to serving for the fertilization of crops, the production of composting also added economic value to farmers' incomes and improved public health, as well as the reduction of germs and parasite diseases. The production of composting of household garbage and livestock food residues can be accelerated using cabbage mud and cattle feces. The use of crab mud can accelerate such composting processes, because in cabbag mud contains microbiological Bacilus sp. aerobic/ anaerobic cutting agents. Adding cattle dirt to the composting process can be useful in accelerating the biodegradation process of household garbage. This ability is due to microorganisms such as protozoa, function, Streptococcus sp. and cellulolithic bacteria, which produce cellulose that can break down (biodegradate) the whole substance in household garbage. The purpose of dedication to the community is to expect the cadres and the community to use the mud and feces of cattle to make compost fertilizer. Research methods use Observational and Socialization to the variables studied. The result of dedication to this community is increasing knowledge about cabbage and cow dirt to accelerate composting on household garbage as well as obtaining compost fertilizer for the community.
Hubungan Pengetahuan, Paritas, Pekerjaan Ibu dengan Keteraturan Kunjungan Ibu Hamil untuk ANC selama Masa Pandemi Covid- 19 Sari, Dewi Indah; Wahyuni, Ninik; Sucipto, Cecep Dani
JURNAL KESEHATAN PRIMER Vol 6 No 1 (2021): JKP (Jurnal Kesehatan Primer)
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/jkp.v6i1.530

Abstract

Abstract: Introduction: ANC is an examination of pregnancy to optimize the physical and mental health of pregnant women, to reduce maternal and child mortality, during the pandemic, although many factors can affect it, including knowledge, parity and maternal occupation. The purpose of this study was to determine the effect of these factors on the regularity of ANC visits to pregnant women in the Covid-19 pandemic era and to determine the most influential factors. Methods: This research was conducted in the Mandala Public Health Center with 91 pregnant women as respondents, or the total population, when analyzing the data could be processed into 83 people. The statistical test used is Chi Square, to determine the most influential factors using logistic regression test with the assumption that the data is normally distributed. Results: The results showed that the relationship between knowledge and regularity of doing ANC was obtained p value = 0.412, which means that there is no significant relationship. The relationship between parity and regularity in conducting ANC obtained a significant value of p value = 0.015, which means that there is a significant relationship between respondents' parity and regularity in conducting ANC. The relationship between work and regularity of doing ANC obtained a significant value of p value = 0.826 which means there is no significant relationship. Conclusion: The regularity of visits by pregnant women to perform ANC is not related to knowledge parity and maternal occupation. This research is expected to be an input to develop a work program in increasing ANC coverage during a pandemic in order to minimize the incidence of maternal and infant mortality. Abstrak: Pendahuluan: ANC merupakan pemeriksaan atas kehamilan untuk mengoptimalkan kesehatan fisik dan mental ibu hamil, untuk menekan angka kematian ibu dan anak, masa masa pandemic meskipun banyak factor yang dapat mempengaruhinya diantaranya pengetahuan, paritas dan pekerjaan ibu. Tujuan dari penelitian ini untuk mengetahui pengaruh factor-faktor tersebut terhadap keteraturan kunjungan ANC pada ibu hamil di era pandemic covid-19 serta mengetahui factor yang paling berpengaruh. Metode: Penelitian ini dilakukan di wilayah kerja Puskesmas Mandala dengan responden 91 ibu hamil, atau total populasi, saat melakukan analisis data data yang dapat diolah menjadi 83 orang. Uji statistic yang digunakan menggunakan Chi Square, untuk mengetahui factor yang paling berpengaruh menggunakan uji regresi logistic dengan asumsi data terdistribusi normal. Hasil: Hasil penelitian diperoleh hubungan pengetahuan dengan keteraturan melakukan ANC diperoleh nilai p value = 0.412 artinya tidak ada hubungan yang signfikan. Hubungan paritas dengan keteraturan melakukan ANC diperoleh nilai signifikan p value = 0.015 artinya ada hubungan yang signifikan antara paritas responden dengan keteraturan melakukan ANC. Hubungan pekerjaan dengan keteraturan melakukan ANC diperoleh nilai signifikan p value = 0.826 berarti tidak ada hubungan yang signifikan. Kesimpulan: Keteraturan kunjungan ibu hamil melakukan ANC tiak berhubungan dengan pengetahuan, paritas dan pekerjaan ibu. Penelitian ini diharapkan sebagai masukan untuk menyusun program kerja dalam meningkatkan cakupan ANC pada masa pandemic guna meminimalisir kejadian kematian ibu dan bayi.