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All Journal Jurnal Ilmu dan Industri Peternakan Jurnal Sain Peternakan Indonesia JURNAL PETERNAKAN Jurnal Peternakan Indonesia AGRIMOR JPP IPTEK (Jurnal Pengabdian dan Penerapan IPTEK) Bakti Cendana: Jurnal Pengabdian Masyarakat Journal of Tropical Animal Science and Technology Livestock and Animal Research JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Jurnal Umum Pengabdian Masyarakat (JUPEMAS) Journal of Community Empowerment Peternakan Abdi Masyarakat (PETAMAS)
Alfred Nubatonis
Program Studi Peternakan Fakultas Pertanian, Universitas Timor
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Economics, Econometrics & Finance Education Environmental Science Nursing Public Health Social Sciences Veterinary Other

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Journal : Journal of Tropical Animal Science and Technology

 1 
Reproductive Organ Profile of Pullet Phase Kampung Chicken After L-Isoleucine Supplementation in Feed Lisnahan, Charles Venirius; Elu, Maria Eriska; Dethan, Agustinus Agung; Nubatonis, Alfred
Journal of Tropical Animal Science and Technology Vol. 6 No. 1 (2024): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v6i1.6102

Abstract

The aim of this research was to determine the effect of supplementation of L-isoleucine in feed on the reproductive organ profile of Native chickens at the pullet phase. This research was carried out from April to July 2023 at Sasi Village, Kota Kefamenanu Sub-District, North Central Timor Regency. A total of 100 Native chickens aged 6 weeks with an average weight of 400 g were used in this study. The research method used was a Completely Randomized Design (CRD) consisting of 5 treatments and 5 replications. Each replication consisted of 4 Native chickens. The treatments given were T0 (feed without L-isoleucine), T1 (T0 + 0.05% L-isoleucine), T2 (T0 + 0.15% L-isoleucine), T3 (T0 + 0.25% L-isoleucine), and T4 (T0 + 0.35% L-isoleucine). The variables observed were the weight of the ovaries, the distance between the 2 pubic bones, the distance between the pubic bone and the sternum, testicular weight and testicular volume. The data obtained were analyzed using analysis of variance and Duncan's test. The results showed that the ovarian weight for T0, T1, T2, and T3 and T4 were 1.14 ± 0.18; 1.43±0.12; 2.26±0.53; 1.76 ± 0.34 and 1.33 ± 0.27 g/head, respectively. The distance between the 2 pubic bones were 21.04 ± 0.49; 22.70±0.61; 25.40±0.82; 23.90 ± 0.46 and 22.08 ± 0.63 mm/head, respectively. The distance between the pubic bone and the sternum were 31.82 ± 1.93; 33.60±1.10; 37.04±2.36; 33.76 ± 1.27 and 31.56 ± 0.93 mm/head, respectively. Testicular weight were 5.56 ± 0.42; 6.15±1.48; 7.58±0.47; 6.80 ± 0.43 and 6.16 ± 0.40 g/head, respectively. Testicular volume were 5.04 ± 0.55; 5.82±0.41; 7.14 ± 0.50; 6.58 ± 0.47 and 6.06 ± 0.28 mL/head, respectively. Statistical analysis showed that treatment had a significant effect on ovary weight, the distance between the 2 pubic bones, the distance between the pubic bone and the sternum, testicular weight and testicular volume in Kampung chickens (P<0.05). It was concluded that the use of 0.15% L-isoleucine in feed gave the best results on ovary weight, distance between 2 pubic bones, distance between pubic bone and breast bone, testicular weight and testicular volume in pullet phase Native chickens.
Isolation and Identification of Bacteria from Cattle Rumen Fluid and Their Application on In Vitro Digestibility of Rice Straw Bira, Gerson Frans; Sio, Stefanus; Batu, Matius S; Pardosi, Lukas; Kia, Kristoforus W; kolo, Maria Magdalena; Nubatonis, Alfred
Journal of Tropical Animal Science and Technology Vol. 7 No. 3 (2025): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aimed to isolate and identify bacteria from the rumen fluid of Bali cattle and to evaluate their effect as microbial inoculants on the in vitro digestibility of rice straw. The experiment was conducted using a Completely Randomized Design (CRD) consisting of four treatments and four replications. The treatments included rice straw silage with the addition of 0%, 5%, 10%, and 15% of local microorganisms (MOL) derived from cattle rumen fluid. The observed variables included the isolation and identification of bacteria from the rumen fluid, and the assessment of in vitro digestibility, including Dry Matter Digestibility (DMD), Organic Matter Digestibility (OMD), Volatile Fatty Acid (VFA) concentration, and ammonia (NH₃) production. The results showed that the addition of rumen fluid MOL significantly (P<0.05) improved the digestibility parameters. The highest DMD (52.44%) and OMD (47.11%) were obtained from the treatment with 15% MOL. VFA and NH₃ concentrations also increased, indicating enhanced microbial activity and fermentation efficiency. The study concludes that MOL derived from cattle rumen fluid, particularly those containing Bacillus sp., is effective in improving the nutritional quality and digestibility of rice straw
Co-Authors Adianus Konsalves Bone Agustina Viktoria Tae Agustinus A. Dethan Agustinus Agung Dethan B. Doarce Banu, Theodorus Batu, Matius S Bere, Yohanes Bernadina Metboki, Bernadina Bira, Gerson Frans Dira Asri Pramita Elu, Maria Eriska Faitnine, Carolina Fransiskus Y. D. Kajdu Fried Markus Allung Blegur Gerson Frans Bira Kia, Kristoforus W Kolo, Maria Magdalena Kristina Irnasari Naikofi Lisnahan, Charles V. Lukas Pardosi Makarius Efi Marselinus Banu Nahak, Adrianus Nailape, Meriana Ngongo Oemboe Warata, Fredrik Nubatonis, Agustinus Oki, Yeremias Pareira, Magdalena Sunarty Pasi, Maria Selfiana Seran, Sofron Sio Stefanus, Sio T. I. Purwantiningsih Thomas Mata Hine Tuas, Maria Angelina Umbu Joka Wiguna, Gede Arya Wilmientje Marlene Mesang Nalley Y. Oki Yuliana Kolo Yuliana Kolo