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Potential of dadiah kapau from Agam District, West Sumatra, Indonesia as a source of probiotics for health ELLY ROZA; SALAM N. ARITONANG; YULIA YELLITA; HILDA SUSANTY; RIZQAN RIZQAN; YUDHA ENDRA PRATAMA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 1 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230161

Abstract

Abstract. Roza E, Aritonang SN, Yellita Y, Susanty H, Rizqan, Pratama YE. 2021. Potential of dadiah kapau from Agam District, Indonesia as a source of probiotics for health. Biodiversitas 23: 564-571. Dadiah is a fermented product of buffalo milk prepared in a bamboo container as a traditional food in the Minangkabau tribe of West Sumatra, Indonesia. This study aimed to identify lactic acid bacteria that are potentially probiotics from dadiah products. The method used in this research includes an experimental survey with descriptive data analysis. The dadiah used in this study was taken from dadiah breeders and processors in Nagari Kapau, Agam District, West Sumatra, Indonesia. Tests for selection of probiotic candidates included macroscopic and microscopic observations, catalase test, fermentative type, resistance to gastric acid (pH 3), resistance to bile salts, antimicrobial activity using test bacteria Escherichia coli O157, Staphylococcus aureus, Listeria monocytogenes and Salmonella sp. Molecular identification was carried out by using 16S rRNA to determine the relationship of species using MEGA version 7.0 application. The study results revealed that the total colonies of dadiah kapau lactic acid bacteria were 128 x 108 CFU/g, and 12 isolates were obtained from the isolation results. Catalase negative for 12 isolates and 9 homofermentative types and 3 including heterofermentative types. Tests for resistance to gastric acid (pH 3) and bile salt resistance, the best viability was 84.61% and 86.51% respectively. The best antimicrobial activity showed a clear zone against the test bacteria E. coli O157, S. aureus, L. monocytogenes, and Salmonella sp. The phylogenetic analysis of the sequencing results shows that the dadiah kapau lactic acid bacteria are closely related to Lactobacillus plantarum.
Characterization of lactic acid bacteria from buffalo dairy product (dadiah) as potential probiotics SALAM N. ARITONANG; ELLY ROZA; YETMANELI YETMANELI; AFRIANI SANDRA; RIZQAN RIZQAN
Biodiversitas Journal of Biological Diversity Vol. 23 No. 9 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230906

Abstract

Abstract. Aritonang SN, Roza E, Yetmaneli, Sandra A, Rizqan. 2022. Characterization of Lactic Acid Bacteria from Buffalo Dairy Product (Dadiah) as Potential Probiotics. Biodiversitas 23: 4418-4423. Dadiah is one of fermented milk from West Sumatra which made from buffalo milk. These product made traditionally by pouring fresh buffalo milk into a bamboo stick covered with banana leaves and fermenting at room temperature for two days. The study's objective was to isolate and identify Lactic Acid Bacteria (LAB) from dadiah as candidates for probiotics. The factors that are considered are LAB Characterization, Biochemical and Microscopic Characterization of LAB Isolate, Bacterial Resistance in Acidic conditions, Bile Salts Resistance, and Antimicrobial Activity. Furthermore the isolate identify by Polymerase Chain Reaction (PCR) using 16S rRNA. Samples of dadiah were collected from five farmers in Tanjung Bonai Village, North Lintau Buo Regency, Tanah Datar District, West Sumatra, Indonesia. Observations were made in four replications, and the data obtained were analyzed descriptively. The total colony of lactic acid bacteria from dadiah was 7x108-23x108 CFU/mL. LAB isolates from dadiah were classified as Gram-positive bacilli. The isolates are resistant to acidic conditions and bile salts and have inhibitory activity against pathogenic bacteria. Identifying the selected isolate using the 16S rRNA showed that the isolate had similarities with Lactobacillus casei strain HDS-01. It can be concluded that dadiah used in this study is as a probiotic food.