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The Effect of Extraction Time Variation on Caffeine Content in Robusta Coffee Extract using Ultrasound-Assisted Extraction Method Anggraini, Amelia Putri; Fildzah Arifah, Mitsalina; Sayyidah Yahya , Fina Ummu; Nurhasanah, Devika
Journal of Food and Pharmaceutical Sciences Vol 13, No 1 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.18750

Abstract

West Lampung Robusta coffee has a higher caffeine content than other types of coffee. The variations in extraction time in ultrasound-assisted extraction (UAE) produced different caffeine contents than conventional extraction methods. This study aims to determine extraction time variation (15, 30, 45) influences caffeine contents in roasted coffee beans. The organoleptic test and alkaloid test evaluated Robusta coffee qualitatively. The extract obtained was measured by measuring the % MC, yield value, and wavelength scanning. Quantitative analysis using UV-Vis Spectrophotometry determined the caffeine content with different extraction times, and then each group was assessed using SPSS software. The coffee bean powder was dark brown, had a distinctive coffee aroma, bitter taste, and smooth texture, and contained alkaloids with the Mayer, Wagner, and Dragendorff tests. The yield and % water content of extract at 30 (36.938%; 6.39%) were higher than at 15 (24.111%; 6.07%) and 45 minutes (22.170%; 6.37%). Alkaloids of the extract were investigated at a wavelength of 273 nm. Caffeine contents were 0.977 ± 0.018 mgCE/mg at the 15, 0.792 ± 0.054 mgCE/mg at the 30, and 0.979 ± 0.026 mgCE/mg at the 45 minutes in significant (P<0.05). In conclusion, the difference in extraction time impacted the caffeine content of the robusta coffee bean.
Penyuluhan Perbedaan Masa Kadaluarsa Obat (ED) dengan Obat Dapat Digunakan Setelah Dibuka (BUD) pada Masyarakat Dusun Kertan Kabupaten Bantul Shalahuddin Al Madury; Arifah , Mitsalina Fildzah; Aldian , Decky; Maulana, M. Fadlhil Rheza
JOURNAL OF PHILANTHROPY: The Journal of Community Service Vol. 3 No. 1 (2025): Journal of Philanthropy, January 2025
Publisher : Samodra Ilmu: Lembaga Penelitian, Penerbitan, dan Jurnal Ilmiah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58353/jop.v3i1.232

Abstract

The proper use of drugs is a challenge in society. The level of public knowledge regarding the proper use of drugs is still low. Many people still do not understand the beyond use date (BUD) of semi-solid preparations. BUD is the limit of the drug is still safe and can be used after the packaging is opened, not the same as the Expired date (ED) listed on the drug packaging. So this can cause misunderstanding and is a new thing for the public to know the BUD of a drug preparation. The community service activity on Saturday, October 5, 2024 at 15.30 WIB took place at the Ainun Jariyah Mosque, Kertan Hamlet, Sumberagung, Jetis, Bantul Regency was attended by 31 residents. After pharmacist education was carried out through lectures, and assisted by leaflet media, there was a difference in the number of participants with a moderate pretest level, namely from 6 to 19 people. Education about ED and BUD accompanied by leaflet media can increase public knowledge as seen from the difference in pretest and posttest which shows the number of participants with an increase in correct answers during the pretest and posttest of 68%.
The Influence of Dry Rendering and Ultrasonic?Assisted Extraction on DPPH Radical Scavenging Activity of Red Fruit (Pandanus conoideus) Arifah, Mitsalina Fildzah; Kurniasih, Kholif Sholehah Indra; Kurniawati, Endah; Erlinaningrum, Mustika; Melanie, Zaskia Nora
MEDIA ILMU KESEHATAN Vol 14 No 2 (2025): Media Ilmu Kesehatan
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/mik.v14i2.1471

Abstract

Background: Red fruit (Pandanus conoideus) is a source of antioxidant compound components. Extraction methods can affect the antioxidant activity of the red fruit oil (RFO). Dry rendering is often used in conventional oil extraction, but it can potentially degrade antioxidant components. Currently, ultrasonic-assisted extraction (UAE) is a modern, yet underexplored method for oil extraction.Objective: This study aims to compare the extraction method on the antioxidant activity of RFO. Methods: The extraction methods included dry rendering and ultrasound-assisted extraction (UAE) using water and n-hexane as solvents. The flavonoid and phenolic contents, percentage yield, and DPPH free radical scavenging antioxidant activity were evaluated in RFO.Results: The results showed that the % yield with dry rendering was higher than in the UAE. The three extracts showed the presence of flavonoids and phenolics based on FeCl3 and AlCl3 tests. The highest antioxidant activity was in the dry rendering method (0,13% w/v) compared to UAE-water (0,98% w/v) and UAE-n hexane (1,47% w/v). Dry rendering could separate bioactive compounds with the highest % yield and inhibit DPPH free radicals with high temperatures, and without solvents. Conclusion: The dry rendering was selected as the extraction method, resulting in higher yields and antioxidant compounds in EBM compared to UAE.
An Integrated FTIR-TLC-PCA Approach for an Accurate Classification Model of Kratom Venation Rusydan, Azka Muhammad; Arifah, Mitsalina Fildzah; Lukitaningsih, Endang; Fakhrudin, Nanang
Majalah Farmaseutik Vol 21, No 3 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v21i3.101780

Abstract

Kratom (M. speciosa), has a long history of traditional use for various ailments as well as for recreational purposes due to its opioid and psychoactive effects. Nowadays kratom is easily accessible via online markets, with leaf powders commonly categorized by vein color, suggesting different effects despite minimal variations in alkaloid content. To improve the identification and characterization of kratom samples, fingerprinting methods using chemometric tools are increasingly applied in food and pharmaceutical analysis. This study explores a combination of Fourier Transform Infrared Spectroscopy (FTIR) and Thin-Layer Chromatography (TLC) densitometry data, analyzed with Principal Component Analysis (PCA), to develop a model for distinguishing kratom venation and other alkaloid-containing plants.The TLC chromatogram revealed six consistent peaks (Rf values of 0.17, 0.27, 0.42, 0.73, 0.8, and 0.9), correlating with alkaloids found in kratom. Using PCA, we combined FTIR absorbance values at selected wavenumbers with TLC chromatogram data, resulting in four principal components (PC1, PC2, PC3, and PC4) that explained 84.1%, 9.7%, 2.7%, and 2.5% of the variance, respectively. The resulting score plot demonstrated distinct clustering of samples, which was then verified with cluster analysis. The resulting analysis indicated effective differentiation between kratom vein colors and plant species. The developed FTIR-TLC-PCA model offers a promising approach for sample classification, potentially aiding quality control and authenticity verification in pharmaceutical applications.
Pengaruh Pemberian Edukasi Apoteker Terhadap Tingkat Pengetahuan Masyarakat Tentang DAGUSIBU di Kabupaten Bantul Shalahuddin Al Madury; Arifah, Mitsalina Fildzah; Maulana, M. Fadlhil Rheza
Jurnal Indonesia Sehat Vol. 3 No. 1 (2024): JURINSE, April 2024
Publisher : SAMODRA ILMU: Lembaga Penelitian, Penerbitan, dan Jurnal Ilmiah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58353/jurinse.v3i1.207

Abstract

Background: Medication error among the community is a major public health problem that can cause great harm to patients. Problems that are often found in the community are the level of understanding of drug use starting from obtaining, using, storing, and destruction of drugs in the correct way. Sustainable efforts are needed to improve public knowledge through the provision of information through educational media by Pharmacists as health workers who understand drugs. Purpose:  This study aims to determine the effect of Pharmacist education on the level of public knowledge in Bantul Regency. Method:  This type of research is pre-experimental with a one group pre-test, post-test design in one group. Analysis using univariate and bivariate using the Wilcoxon test. Result:  The results showed an increase in the percentage of respondents' knowledge level in the good category after receiving educational treatment with the lecture method and assisted by leaflet media, from 27.5% to 52.5%, and a decrease in the level of knowledge is less, at the pre-test 27.5% to 15% during the post-test conducted after education was carried out. Conclusion:  The provision of education materials by pharmacists has a significant effect on increasing knowledge about DAGUSIBU in the community in Bantul Regency with a p-value (0.021) <0.05.
Education of Herbal Soap Homemade in Dukuh Kertan, Sumberagung, Bantul: Edukasi Pembuatan Sabun Herbal di Dukuh Kertan, Sumberagung, Bantul Arifah, Mitsalina Fildzah; Shalahuddin Al Madury; Nurul Afniatun; Citra Eka Runenti
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 9 No. 2 (2025): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v9i2.21539

Abstract

Herbal soap is the saponification process of natural ingredients from medicinal plants. Herbal ingredients are minimally unknown by the people of Pedukuhan Kertan to make natural cosmetics. Herbal soap is successful in being environmentally friendly, safe against detergent allergies, and increasing the economic value of herbal ingredients in the community. Education in making herbal soap was needed for the people of Dukuh Kertan, Sumberagung, and Bantul in 2024 to implement health promotion through community empowerment. Community service activities were determined using direct counseling and training methods. The community service targeted Family Welfare Empowerment (PKK) and Youth organization in Dukuh Kertan. The series of activities began with counseling on education on making herbal soap, practical training, and competitions between community groups in each RT with various variations of materials according to creativity. Community service evaluations were carried out in the form of a pretest and posttest carried out by 27 people. The results of the pretest on community knowledge of herbal soap were 13 (excellent), 6(understand), 4 (quite knowledgeable), 2 (less knowledgeable), and two people (ignorant). The knowledge of participants in the posttest increased by 24 (excellent), 2 (understand), and one people (quite knowledgeable). Herbal soap could display the creativity of each group of the community. Therefore, making herbal soap could increase knowledge and skills of herbal soap products through community empowerment.
Identifikasi Kandungan Formalin Pada Tahu Putih Di Pasar Tradisional Kecamatan Klaten Tengah dengan Metode Spektrofotometri UV-Vis Alifia Nuraini; Meila Diana; Arifah, Mitsalina Fildzah
CERATA Jurnal Ilmu Farmasi Vol 16 No 2 (2025): Cerata Jurnal Ilmu Farmasi
Publisher : Universitas Muhammadiyah Klaten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61902/cerata.v16i2.1856

Abstract

White tofu is a high-protein food widely consumed by the public but has a short shelf life due to its high water content. To extend its shelf life, some vendors use formalin as a preservative, although its use is prohibited due to its toxic nature and potential to cause digestive disorders, organ damage, and cancer. Traditional markets in Central Klaten District serve as economic centers for the local community; however, information regarding the presence of formalin in white tofu remains limited. This study aimed to identify presence of formalin in white tofu sold at these markets, qualitatively and quantitatively. A non-experimental descriptive design was used, involving organoleptic testing over three days, qualitative analysis using KMnO4, Fehling A & B, Schiff, and chromotropic acid reagents, determination of the maximum wavelength, and quantitative analysis using the UV-Vis spectrophotometry method. Signs of spoilage were observed in all samples through organoleptic testing. No colour change was detected in qualitative analysis, and no absorption peak was observed at 580 nm. The validity of the UV-Vis spectrophotometry method was demonstrated by a linearity and linear range with r = 0.999, %Recovery from accuracy of 97.807-101.023%, %RSD from precision were 1.206%, a limit of detection (LOD) of 2.811 ppm, and a limit of quantification (LOQ) of 8.519 ppm. Samples suspected of containing formalin were confirmed to be free of formalin based on the quantitative test. The limitation of this study was its lack of sensitivity toward very low formalin concentrations. In conclusion, formalin was not detected in any sample through organoleptic, qualitative, and quantitative test. The validity of the UV-Vis spectrophotometry method met the required validation parameters.