Article Per Year (5 Year)
p-Index From 2020 - 2025
0.92
P-Index
This Author published in this journals
All Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Kultivasi Agrinimal Jurnal Ilmu Ternak dan Tanaman JAS (Journal of Animal Science) Farmers: Journal of Community Services
Romi Zamhir Islami
Fakultas Peternakan Unpad, Jl. Raya Jatinangor Km 21 Bandung 40600
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Social Sciences Veterinary Other

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
The effect of various doses of gamma ray irradiation on growth and chlorophyll changes of three adlay genotypes Qosim, Warid Ali; Anas, Anas; Amien, Suseno; Rachmadi, Meddy; Ramdani, Sidik; Islami, Romi Zamhir
Kultivasi Vol 23, No 2 (2024): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v23i2.55619

Abstract

Adlay has great potential to be developed into an alternative food source in Indonesia because it contains carbohydrates and high fat. The aim was to effect of various doses of gamma-ray irradiation on growth,chlorophyll changes, and determination of lethal dose (LD50) three adlay genotypes in M1 generation. This study was conducted by using the experimental quantitative method without design. The material used consists of three adlay genotypes, including genotypes #28, #37, and #38. The seeds were treated with 0 Gy (control), 100 Gy, 200 Gy, 300 Gy, 400 Gy, and 500 Gy doses of gamma-ray irradiation by the Research Center for Radiation Process Technology, the National Research and Innovation Agency using Gamma Cell 220. The research was conducted at the Faculty of Agriculture Experimental Station, Universitas Padjadjaran, from December 2017 to July 2018. The  LD50 was calculated by the curve-fitting analysis program based on characters of survival percentage, height seedling, root length, and appearance of leaf chlorophyll change in the three adlay genotypes. The results showed that the treatment of gamma-ray irradiation gave a diverse response to characters of survival percentage, height seedling and rooting length of the three adlay genotypes. There were LD50 for each genotype, 346 Gy for genotype #28, 381 Gy for genotype #37, and 371 Gy for genotype #38. The optimum dose of gamma-ray irradiation for the three adlay genotypes was 300 Gy. The appearance of chlorophyll change leaf the three adlay genotypes caused by gamma ray irradiation treatments with doses of 100 - 500 Gy were able to produce variation in the spectrum and frequency of different chlorophyll change in the M1 generation, the irradiation treatment of 400 Gy dose was able to make the highest frequency of chlorophyll mutations with a total frequency of 46.28%. Meanwhile, the gamma-ray irradiation treatment of 300 Gy produced the broadest chlorophyll mutant spectrum with 6 types of chlorophyll change consisting of tigrina, striata, viridis, variegata, maculata and albina green.
Microsatellite (RM20A, RM241, and RM315) Amplification of Panic Grass DNA (Panicum maximum) Gananto, Adrian Ramadhan; Islami, Romi Zamhir; Mansyur, Mansyur; Pramudyawardani, Estria Furry
JAS Vol. 9 No. 4 (2024): Journal of Animal Science (JAS) - Oktober 2024
Publisher : Program Studi Peternakan, Fakultas Pertanian, Sains dan Kesehatan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Forage is a very important element for livestock development as they need it to survive, produce and reproduce. The forages that is often used is panic grass (Panicum maximum) which is a feed grass from the African plains, widely used in various regions of Indonesia. This grass is used for their high nutritional value, the ability to adapt various types of soil and weather, and usage as an ideal or high quality “cut and carry” grass for beef and dairy cattle. Forage cultivation by considering plant genetics can improve the quality of all types of forage inherited from the parent hence increase the productivity of feed crops and support livestock development. This can be done with molecular marker, which is a technology that can confirm target genes effectively, efficiently and accurately and are not influenced by environmental factors. Among the markers or molecular markers currently available, microsatellites or Simple Sequence Repeats (SSR) have become a marker system that is commonly used because of its various advantages, namely that it can be amplified by Polymerase Chain Reaction using primers that combine sequences in the target DNA. In this research, genes that carry leaf-rolling, plant height, and fertility-restoring properties that have previously been identified in rice plants will be tested on panic grass using RM20A, RM241, RM315. After being visualized using electrophoresis gel, it can be concluded that RM241 had the best result as it has clear amplification result and polymorphic bands with effectiveness and polymorphism levels of 75% and 100% respectively.
Eskalasi Pengetahuan Bahan Pakan dan Formulasi Ransum Ternak Ruminansia Bagi Peternak Milenial di Jawa Barat Christi, Raden Febrianto; Islami, Romi Zamhir; Febrianto, Firman; Sudrajat, Ajat
Farmers: Journal of Community Services Vol 6, No 1 (2025): Farmers: Journal of Community Services
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/fjcs.v6i1.60409

Abstract

Pemahaman bahan pakan dan formulasi ransum perlu ditingkatkan pada peternak ruminansia. Semakin mengetahui jenis bahan pakan maka akan tepat pula dalam proses penyusunan ransumnya. Tujuan dari penelitian ini adalah eskalasi pengetahuan tentang bahan pakan dan formulasi ransum menjadi solusi yang sangat diperlukan untuk meningkatkan produktivitas ternak ruminansia di Jawa Barat. Kegiatan telah dilaksanakan pada bulan November 2024 yang bertempat di Kota Bandung dan diikuti sebanyak 70 peternak milenial dari seluruh perwakilan Kabupaten di Jawa Barat. Metode yang digunakan dalam kegiatan ini diantaranya  tahapan persiapan, penyuluhan dan demonstasi, serta evaluasi pre-test dan post test. Langkah awal yang dilakukan meliputi identifikasi masalah, kajian literatur sebagai dasar pemecahan masalah, serta persiapan terkait perizinan, lokasi, dan jadwal pelaksanaan. Tahap selanjutnya mencakup penyuluhan dan demonstrasi. Hasil menunjukkan bahwa terjadi peningkatan kegiatan penyuluhan pada pengertian pakan  100%, bahan pakan ruminansia 100% yang semula 92,85%, penggolongan nutrien pakan 100% semula 85,71%,  kebutuhan nutrien pakan 93,33% semula 35% dan teknik formulasi ransum 93,33% semula 35,71%.  Terjadi peningkatan pengetahuan dan pemahaman terhadap bahan pakan dan formulasi ransum ternak ruminansai pada peternak milenial di Jawa Barat mulai pengertian pakan, bahan pakan, penggolongan nutrien pakan, penggunaan kebutuhan nutrien pokok dan teknik formulasi ransum.
KONSENTRASI DAN KEMURNIAN DNA HASIL EKSTRAKSI RUMPUT ODOT, JAGUNG HIBRIDA, PADI IR64 DAN PADI CIHERANG Rahmah, Vita Amalia; Tanuwiria, Ujang Hidayat; Islami, Romi Zamhir
Agrinimal Jurnal Ilmu Ternak dan Tanaman Vol 13 No 2 (2025): Agrinimal Jurnal Ilmu Ternak dan Tanaman
Publisher : Jurusan Peternakan Fakultas Pertanian Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajitt.2025.13.2.119-126

Abstract

Pemahaman terhadap karakteristik genetik tanaman sangat dibutuhkan dalam pengembangan varietas unggul guna mendukung ketersediaan pakan ternak berkualitas, yang salah satunya dapat dilakukan melalui analisis DNA. Penelitian ini bertujuan untuk menganalisis konsentrasi dan kemurnian DNA empat jenis tanaman pakan, yaitu rumput odot, jagung hibrida, padi IR64, dan padi Ciherang. Metode yang digunakan dalam penelitian ini meliputi ekstraksi DNA menggunakan Genomic DNA Mini Kit (Plant) dari Geneaid, diikuti dengan pengukuran konsentrasi dan kemurnian DNA menggunakan spektrofotometer NanoDrop. Hasil penelitian menunjukkan bahwa DNA dari seluruh sampel memiliki konsentrasi yang cukup untuk analisis molekuler, yaitu antara 12,5 hingga 75,9 ng/μL. Rasio A260/A280 menunjukkan bahwa DNA jagung hibrida, padi IR64, dan padi Ciherang memiliki tingkat kemurnian yang sesuai standar (1,8–2,0), sementara DNA rumput odot menunjukkan rasio kemurnian rata-rata 1,44 berada di bawah standar kemurnian dan mengindikasikan adanya kontaminasi protein. Dengan demikian, metode ekstraksi menggunakan kit dari Geneaid cukup efektif dalam menghasilkan DNA berkualitas dan siap untuk dilakukan analisis lebih lanjut meskipun untuk rumput odot perlu optimasi lebih lanjut guna memperoleh DNA dengan kemurnian optimal. ABSTRACT Understanding the genetic characteristics of these plants is essential for the development of superior varieties to ensure the availability of quality animal feed, which can be achieved through DNA analysis. This study aimed to analyze the concentration and purity of DNA from four types of forage crops: Pennisetum purpureum cv. Odot (odot grass), hybrid corn, IR64 rice, and Ciherang rice. The methods used included DNA extraction using the Geneaid Genomic DNA Mini Kit (Plant), followed by measurement of DNA concentration and purity using a NanoDrop spectrophotometer. The results showed that all samples yielded DNA with sufficient concentration for molecular analysis, ranging from 12.5 to 75.9 ng/μL. The A260/A280 ratio indicated that the DNA from hybrid corn, IR64 rice, and Ciherang rice met the standard purity range (1.8–2.0), while the DNA from odot grass showed an average purity ratio of 1.44, suggesting protein contamination. Therefore, the Geneaid extraction kit was effective in producing high-quality DNA suitable for further molecular analyses, although additional optimization is required for odot grass to obtain DNA with optimal purity.
Application of the DNA Molecular Markers RM20A and RM241 on King Grass (Pennisetum purpuphoides) and Dwarf Elephant Grass (Pennisetum pupureum cv. Mott) Islami, Romi Zamhir; Ari Zindhi, Muhammad Amrullah; Mansyur; Susilawati, Iin
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 2 (2024): August 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.02.15

Abstract

Feed, as the main element that provides livestock nutrition, is composed of two components, namely, concentrate and forage. Examples of commonly used forages are king grass and dwarf elephant grass. These grasses are very familiar among farmers because they have good crude protein (CP) content; specifically, the CP content of king grass is 13,50% (Siswanto et al., 2016), and the CP content of dwarf elephant grass is 14,35% (Erleli, 2020). Forage production can be disrupted by weather factors. In the dry season, the production of king grass and dwarf elephant grass decreases by 50%. As a result, there is a forage crisis in the dry season. To overcome this, genetic improvement of king grass and dwarf elephant grass is possible. Previous research has succeeded in finding primers RM20A and RM241, each of which can lead to new traits in rice plants, namely, leaf rolling and plant height. This primer can be applied to king grass and dwarf elephant grass to increase the production potential of these plants and increase plant resistance in the dry season. This research uses the PCR technique to amplify primers from the template DNA of king grass and dwarf elephant grass. Optimization was carried out via several procedures due to species differences among rice, king grass and dwarf elephant grass. The results revealed that the RM241 primer could not be amplified at all in king grass plants and was amplified only in dwarf elephant grass. Moreover, the RM20A primer produced better results, where amplification could occur in both plants. This may be due to several factors, such as the condition of the DNA template, the condition of the materials and the possibility of human error. Amplification of the two primers also revealed that they were polymorphic, indicating that these two primers could not accurately amplify SSRs in king grass and dwarf elephant grass. This also causes many smears in the visualization of the PCR results. Overall, this research was able to determine the plant height and leaf rolling characteristics of king grass and dwarf elephant grass.
Co-Authors Aminuyati Anas Anas, Anas Ari Zindhi, Muhammad Amrullah Christi, Raden Febrianto Febrianto, Firman Gananto, Adrian Ramadhan Iin Susilawati Mansyur Mansyur Mansyur Meddy Rachmadi Pramudyawardani, Estria Furry Rahmah, Vita Amalia Ramdani, Sidik Suseno Amien Ujang Hidayat Tanuwiria WARID ALI QOSIM