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All Journal HAYATI Journal of Biosciences Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) Jurnal Perikanan Universitas Gadjah Mada Aquacultura Indonesiana Journal of Aquaculture and Fish Health Paedagogie: Jurnal Pendidikan dan studi ISlam SIGANUS: Journal of Fisheries and Marine Science Journal of Fisheries & Marine
INDAH ISTIQOMAH
Departemen Perikanan, Fakultas Pertanian, Universitas Gadjah Mada, Yogyakarta, Indonesia
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Social Sciences Other

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Journal : Journal of Fisheries

 1 
Safety, Adherence, Enzymatic Activities, and Application Effects of Oral Probiotic Candidates for Shortfin Eel (Anguilla bicolor bicolor) Andita Ratih Dewanti; Anggi Octari Putri; Indah Istiqomah Istiqomah; Alim Isnansetyo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i2.34315

Abstract

Highlight Research The shortfin eel elver bicolor bicolor was tested for the safety of Enterobacter sp. JC05, Lactococcus sp. JAL37, and Bacillus sp. PCP1 The ability of bacterial strains to adhere to epithelial cells of shortfin eel epithelial cells was demonstrated The bacterial strains' proteolytic, cellulolytic, and lipolytic activities were detected Oral administration of the bacterial cocktail lowered overall viable bacterial count but did not affect the shortfin eel's intestinal histological characteristics   Abstract Aquaculture of the shortfin eel (Anguilla bicolor bicolor) has been plagued by low survival and growth due to the low tolerance to water quality and feed. The microbiota and shape of the fish intestinal tract influence the immune and digestive systems. The use of bacterial probiotics is fascinating to enhance the digestion system. This study aimed to characterize bacterial probiotic candidates' safety and potential probiotic features for shortfin eel (A. bicolor bicolor) aquaculture. The safety, adherence, and enzymatic activity of three bacterial strains (Bacillus sp. PCP1, Lactococcus sp. JAL 37, and Enterobacter sp. JC05) were investigated. An oral application test was performed on shortfin eel (n=880, 15 g) every four days with 0, 3x103, 3x105, and 3x107 CFU/g diet dosages in quadruplicates for two months. At the end of the experiment, total cultivable bacteria and intestinal morphology were assessed. Based on the hemolytic test and intraperitoneal injection, the bacterial strains were considered harmless. In an in vitro investigation, the bacteria attached to shortfin eel intestinal epithelial cells. An agar diffusion method validated the proteolytic, lipolytic, and cellulolytic activities. In vivo feeding tests with the bacterial cocktail lowered the total viable bacteria in the gut while preserving the gut histology. Bacterial strains of the present study are potential probiotic candidates for shortfin (A. bicolor bicolor) aquaculture.
Sodium Alginate from Padina australis Modulates Innate Immune and Immune Gene Expression in Red Tilapia (Oreochromis sp.) Toni Kuswoyo; Alim Isnansetyo; Muwantoko Murwantoko; Amir Husni; Indah Istiqomah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i1.40943

Abstract

Highlight Research Development of immunostimulant from natural sources is a promising approach to improve the immune status of fish Dietary administration of sodium alginate from Padina australis at 2.0 g/kg was an effective immunostimulant for red tilapia Red tilapia fed diet with sodium alginate significantly increased innate immune responses of TLC, PA, respiratory burst, SOD, TPP, lysozyme, and ACH50 activity Red tilapia fed diet with sodium alginate significantly modulated immune genes expression of GPx, TNF-α, IFN-γ, IL-10, TGF-β1, and Hepcidin Abstract The study of immunostimulant derived from natural sources has received considerable attention in recent years. Indonesian coasts have various sources of immunostimulant, especially brown algae, which are rich in alginate. This research investigated innate immune response and immune genes in red tilapia (Oreochromis sp.) after being fed diets supplemented with sodium alginates (SA) from a brown alga, Padina australis, originating from Awur Bay, Jepara, Indonesia. This work provided comprehensive information regarding the interaction of cellular-humoral immunity and immune gene expression. Red tilapia was fed with SA-containing diet at doses of 0, 2, 3 and 4 g/kg for 12 days, then blood samples were collected on days 0, 3, 6, 9, and 12 to evaluate the total leucocyte count (TLC), phagocytic activity/index (PA/PI), respiratory burst, superoxide dismutase (SOD), lysozyme, alternative complement (ACH50) activity, total plasma protein (TPP), and immune genes expression (blood sample on day 6). The immunological parameters improved in response to the administered doses and period. Dietary SA enhanced total leucocyte count, phagocytic activity, respiratory burst, superoxide dismutase, lysozyme, and ACH50 activity, whereas the phagocytic index and total plasma protein were not significantly different. Furthermore, the mRNA levels of antioxidant-related gene (GPx), pro-inflammatory cytokines (TNF-α and IFN-γ, except for IL-1β), and antimicrobial peptides (Hepcidin) were upregulated. Meanwhile, anti-inflammatory cytokines (IL-10 and TGF-β1) were downregulated. Supplementation SA diet at 2.0 g/kg as the lowest dose revealed the most effective effects on innate immune and immune genes expression. Dietary SA was a potential immunostimulant in red tilapia aquaculture.
Susceptibility and Target Organ of Lymphocystis Disease Virus Infection in Giant Gourami (Osphronemus goramy), Hybrid Tilapia (Oreochromis sp.), Siamese Fighting Fish (Betta splendens), and Hybrid Catfish (Clarias sp.) Nikmah, Nur Lailatul Fitrotun; Isnansetyo, Alim; Istiqomah, Indah; Murwantoko, Murwantoko
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.56685

Abstract

  Highlight Research 1. Lymphocystis disease is reported to infect seawater and freshwater fishes 2. The four important freshwater fish species in Indonesia are evaluated on their susceptibility to Lymphocystis Disease Virus 3. Lymphocystis infection causes behavioural changes and mortality with different onset times after infection on the four fish species 4. LCDV load is varied in quantity among different organs. Abstract Lymphocystis disease has a broad host range and has been reported to enter Indonesia. However, information regarding its susceptibility and predilection organs in fish is lacking. This study examined the susceptibility of four important fish species in Indonesia, namely, giant gourami (Osphronemus goramy), hybrid tilapia (Oreochromis sp.), Siamese fighting fish (Betta splendens), and hybrid catfish (Clarias sp.). The fish were infected with virus filtrate by intraperitoneal injection and immersion. The postinfection observation period was 60 days. Viral load was quantified by qPCR and expressed as major capsid protein (MCP) copy number/mg tissue. Mortality was observed in all fish species, with the highest recorded in hybrid catfish and the lowest in Siamese fighting fish. All the fish species showed changes in their clinical symptoms, such as anorexia and separation from schools. However, only giant gourami showed internal change seven days after injection (dpi), with white lesion detected in the liver. Viral load quantification showed that LCDV had different predilection organs in the four fish species. The highest viral load of giant gourami (1.7 x 104) was observed in the liver at 7 dpi, hybrid tilapia (7.5 x 103) was observed in the fins at 21 dpi, Siamese fighting fish (8.4 x 103) was observed in the fins at 14 dpi, and hybrid catfish (1.2 x 103) were observed in the fins and gills at 7 and 14 dpi. The findings indicated that giant gourami, hybrid tilapia, Siamese fighting fish, and hybrid catfish were susceptible to LCDV infection with different predilection organs.
Effect of Dietary Probiotic Lactococcus garvieae JAL37 Supplementation on Growth and Disease Resistance in Tor tambroides Challenged with Aeromonas hydrophila PTF3 Mahasin, Faiz; Istiqomah, Indah; Handayani, Desy Putri; Isnansetyo, Alim; Murwantoko, Murwantoko; Herath, Tharangani
Jurnal Ilmiah Perikanan dan Kelautan 2026: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2026)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i2.82534

Abstract

Graphical Abstract Highlight Research 1. Aeromonas hydrophila PTF3 was identified and analysed as a causative agent of a previously identified hemorrhagic syndrome in Tor tambroides. 2. Lactococcus garvieae JAL37 supplementation in feed can enhance the growth of Tor tambroides fingerlings. 3. Lactococcus garvieae JAL37 can increase the resistance of Tor tambroides fingerlings to Aeromonas hydrophilla PTF3. 4. Relative mRNA levels of ghrelin, GH, and I-FABP genes were increased after 90 days of tor cultivation with Lactococcus garvieae JAL37 supplementation.   Abstract  Mahseer, or tor fish, an indigenous Asian fish of great socio-economic value, is gaining attention as it faces threats to its survival. However, slow growth rates and disease susceptibility remain significant challenges in mahseer cultivation. Feed supplemented with probiotic Lactococcus garvieae JAL37 at a concentration of 1.0 × 10⁸ CFU/g feed, twice weekly, was used in this study to evaluate its effects compared with a control. Triplicate groups of Tor tambroides fingerlings (average weight 1.86 ± 0.09 g) were cultivated for 90 days. Parameters such as growth, intestinal histology, and mRNA expression levels of growth-related genes (ghrelin and I-FABP from the intestine; GH and IGF-1 from the liver) were assessed. Day 90 post-cultivation, mahseer were challenged with an oral infection of Aeromonas hydrophila at 5.0 × 105 CFU/g fish to assess survival rate and mRNA expression levels of pro-inflammatory cytokine-related genes (TNF-α and IL-1β). Weight gain, specific growth rate, and villus length in Tor tambroides fingerlings treated with probiotics showed significant increases (P<0.05). Real-time PCR results indicated upregulated mRNA expression of ghrelin, I-FABP, and GH genes in the probiotic group, whereas IGF-1 gene expression was similar to that in the control group. Probiotic treatment significantly improves the survival rate of T. tambroides after A. hydrophila challenge, while suppressing mRNA expression of TNF-α and IL-1β genes post-challenge. These findings suggest that Lactococcus garvieae JAL37 supplementation can promote growth in Tor tambroides fingerlings and enhance disease resistance against Aeromonas hydrophila.
Co-Authors . MUHTADI . TRIYANTO Abdullah, Faisal Zain Ahmad Fauzi Rohman Alim Isnansetyo Alim Isnansetyo ALIM ISNANSETYO Alim Isnansetyo Alim Isnansetyo Alim Isnansetyo Alim Isnansetyo Alim Isnansetyo ALIM ISNANSETYO Amir Husni Andita Ratih Dewanti Anggi Octari Putri ANIK KUSMIATUN Annabila, Sayyidah Rahmah Arsana, I Made Putra Aulia, Umu Intan Bambang BU Bambang Genjik S Bambang Triyatmo Buana Basir Deendarlianto Handayani, Desy Putri Hanifudin, Hanifudin Hariyadi, Dimas Rizky Herath, Tharangani Idawati, Khoirotul Ign. Hardaningsih Imelda Novita Atitus Ismail, Norshida Jabbar, Firmansyah Bin Abd Kamiso H. Nitimulyo Kamiso Handoyo Nitimulyo Kamiso Handoyo Nitimulyo KAMISO HANDOYO NITIMULYO Karunia Adetera Nungki Wijayanti Kim, Sung Sam Latifah, Ma'rufa Nurul Mahasin, Faiz Muhammad Murdjani Muhammmad Murdjani Murti, Fauziah Arelia Usmiara Murwantoko . Murwantoko Murwantoko Musyafa, Abriel Ainani Muwantoko Murwantoko Nur Lailatul Fitrotun Nikmah Retno Widaningroem Riesa Yosita Rina Anggraeni Safia Rozi Rozi Rustadi Rustadi Senny Helmiati Senny Helmiati Shima Bhaskara Ayuningrum Syakuri, Hamdan Toni Kuswoyo Triyanto Triyanto Triyanto Triyanto Triyanto Triyanto Wahyudi Wahyudi Wiratni Budhijanto Yasa, Ngurah Sedana