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All Journal METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS Biogenesis: Jurnal Ilmiah Biologi

MADE SUSUN PARWANAYONI

Jurusan Biologi, FMIPA, Universitas Udayana, Denpasar, Bali.

Author-ID : 2925730

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology

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PEMBERIAN EKSTRAK BUAH JUWET (Syzygium cumini L.) TERHADAP JUMLAH DAN MORFOLOGI SPERMATOZOA TIKUS PUTIH (Rattus sp.) JANTAN YANG TERPAPAR ASAP ROKOK Fitria Dwijayanti; A.A.S.A. Sukmaningsih; , Ni Made Rai Suarni; Sang Ketut Sudirga; Ni Made Susun Parwanayoni
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

The research to determine the effect of fruit extracts juwet on the number and morphology of spermatozoa were superbly exposed to smoke has been conducted. This study uses rats aged 3 month, 200-210 gram, total 24 rats consisting of 4 treatment, is control, smoke of cigarette, juwet fruit extracts, and smoke of cigarette to juwet fruit extracts. Each treatment consisting 6 replications, each consisting of 1 rat. Before, acclimatization of rat treated 7 days. Way of giving juwet fruit extracts the gavage method 2 ml once daily for 48 days, while the CMC-Na 0,5% is given control. The exposure to cigarette smoke is given from an aerator pump , to given once daily for 48 days. Data were analyzed with ANOVA, if they were 5% significantly different would be followed by a DMRT. The result showed that the number of abnormal sperm morphology were significantly diferent (p<0,05). The averange number of abnormal spermatozoa K1 group was higher than K3 group. The number of spermatozoa was not significant different between treatments. The average number of spermatozoa K1 lower than K3. This is because the smoke of cigarette increased formation of ROS and the resulting stress oxidative and cause cell damage tissues and organs, especially to the reproductive system. Juwet fruits extract have been able to improve the count and morphologyof rats spermatozoa expose to smoke. This case because of antioxidant from juwet fruit can be protect cell from the free radical attack. Keywords: Rattus sp, smoke of cigarette, java plum fruit, spermatozoa, , ROS, antioxidant.

UJI DAYA HAMBAT ASAP CAIR TEMPURUNG KELAPA (Cocos nucifera L.) TERHADAP PERTUMBUHAN Escherichia coli SECARA In Vitro Rachel Daniella Dinda Maria Lumban Tobing; Made Ria Defiani; Ni Made Susun Parwanayoni
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Meat contaminated with bacteria has the potential to cause dangerous diseases when consumed by humans. One of the germs, especially bacteria that contaminate raw meat or meat with an incomplete maturation process is Escherichia coli. Therefore, E. coli needs to be minimized by inhibiting its growth. One natural way to inhibit the growth of E. coli is to test for antibacterials using liquid smoke. Liquid smoke can be obtained through the pyrolysis process from various biomass containing cellulose, hemicellulose, and lignin such as coconut shell (Cocos nucifera L.). Liquid smoke contains phenolic and acid compounds which are antimicrobial and antioxidant. This research uses the diffusion well method. Data analysis used Completely Randomized Design (CRD) with variance test (ANOVA). If the data had a significant difference at the 5% test level (P?5), the Duncan test was continued. Coconut shell liquid smoke is able to inhibit the growth of E. coli which is indicated by the formation of an inhibition zone. The concentration of coconut shell liquid smoke which effectively inhibited the growth of E. coli was a concentration of 50% with an inhibition zone diameter of 16.66 mm and MIC at a concentration of 10% with an inhibition zone diameter of 9.33 mm. Phytochemical test results of coconut shell liquid smoke contain positive phenolic compounds, flavonoids, triterpenoids, and saponins as well as phenol content of 2.403% and saponin content of 5.50%.

KANDUNGAN LOGAM BERAT (Pb dan Cd) Pada Sawi Hijau (Brassica rapa l. Subsp. Perviridis Bailey) Dan Wortel (Daucus Carrota L. Var. Sativa Hoffm ) YANG BEREDAR DI PASAR KOTA DENPASAR Deni Agung Priandoko; Ni Md Susun Parwanayoni; I Ketut Sundra
SIMBIOSIS Vol I, No 1, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.839 KB)

Has been doing a research about the content of heavy metals (Pb and Cd)in green mustard (Brassica rapa L. subsp. Perviridis Bailey) and carrot (DaucusCarrota L. Var. Sativa Hoffm) that exist in the market of Denpasar city. Purposeof this research was to know the content of Pb and Cd in green mustard andcarrots that exist in traditional markets (Badung and Kreneng market) Denpasarcity that treated by washing and without washing. Content of Pb and Cd in greenmustard and carrots that analyzed by AAS (atomic absorption spectrophotometer).The results of research shows that contents of Pb in green mustard andcarrots that washed and without washed in Kreneng and Badung Market equal to64.71 ± 6.66 ug / g, 69.58 ± 4.61 ug / g, 62.56 ± 6.99 ug / g, 64.96 ± 7:20 ug / g,62.56 ± 6:56 ug / g, 73, 91 ± 2:51 ug / g, 57.17 ± 8:59 ug / g, 59.71 ± 8.93 ug / gdry weight. Content of Cd that washed and without washed equal to SCK 8.81 ±1.68?g / g, 10, 55 ± 1.78 ug / g, 8.09 ± 1.71 ug / g, 9.30 ± 2:01 ug / g, 8.96 ± 1.72ug / g, 10.09 ± 1:09 ug / g, 7.39 ± 1.6 ug / g 5, 8.14 ± 1.71 ug / g dry weight

KUALITAS SATE LILIT YANG BEREDAR DI PASAR TRADISIONAL KARANGASEM, BALI DITINJAU DARI Staphylococcus aureus I Gusti Ayu Laras Pratama Putri; Ida Bagus Gede Darmayasa; Ni Made Susun Parwanayoni
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.207 KB)

This research aims to find out the quality of curled satay in terms of Staphylococcus aureusthat are spread around in the traditional markets of Karangasem, Bali. Samples of the curled satay were taken from the 4 regions in Karangasem (Selat, Rendang, Sidemen, and Seraya), every region takes 1 traditional market and every market are owned by 2 salesman. The method used in this study is the method of Platting Method (Pelczar and Chan, 2005). The results shows that the highest of curled satays in traditional markets of Karangasem is found in S. aureusbacteria was obtained for up to 73 x 102 CFU/g found in Sinduwati market, Sidemen on salesman 1 and 57 x 102 in Seraya Tengah market, on salesman 1. Based on the tests that has been done shows that a number of curled satay sold in traditional markets of Karangasem, Bali has exceeded the threshold amount Indonesian National Standard. The maximum lilit of microbial impurities in satay ready consumption for S. aureusbased on Indonesian National Standard is 102CFU/g. Keywords :Curled satay,Platting Method, Staphylococcus aureus, traditional market

POTENCY OF Trichoderma asperellum TKD FILTRATE IN INHIBITING Aspergillus parasiticus CONTAMINATION IN PEANUT SEEDS (Arachis hypogaea L.) Nasha Hadi Rahmadanty; Ida Bagus Gede Darmayasa; Ni Made Susun Parwanayoni
SIMBIOSIS Vol 11 No 1 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i01.p02

iThis researchhaimed to determineethe potency off Trichoderma asperellum TKD ffiltrate in inhibiting Aspergillus parasiticus contamination in peanut seeds. The treatment given to A. parasiticus was done in vitro and in vivo. In vitro there were three types methods, dual culture and the inhibition test of the T.asperellum TKD ifiltrate using the diffusion well and the colony method with concentrations of 40% (v/v), 50%(v/v), 60%(v/v),70%(v/v) and 80%(v/v). While in vivo, it was done on peanut seeds added T.asperellumiTKD filtrate with some concentrations similar to the treatment given in vitro. The parameters measured in vitro test were determined through PIRG (Percentage Inhibition of Radial Growth) and diameter inhibition zone, in vivo test parameter was the A. parasiticus colony populations. The results showed T.asperellum TKD filtrate significantly (P?0,05) was able to inhibit A. parasiticus growth iin vitro and iin vivo. The percentageeof T.asperellum TKD bland power against A.parasiticus in vitro with the highest dual culture method was 60.65%. In the method of colony and diffusion well, the concentration of 80% was the highest concentration in inhibiting A. parasiticus, which were 83.00±1.60% and 15.09±0.12 mm. T.asperellum TKD filtrate with a concentration of 80% added to peanut seeds had the highest inhibition on the number of population of A. parasiticus colonies with a percentage decrease of 88.3%.

THE POTENTIAL OF CLOVE LEAF EXTRACK (Syzygium aromaticum L.) AGAINST GROWTH OF FUNGAL Rhizoctonia solani Kuhn. CAUSE OF BLIGHT DISEASE IN RICE PLANTS (Oryza sativa L.) I Gusti Ayu Diana Meirani; Ni Made Susun Parwanayoni; Ni Luh Suriani
SIMBIOSIS Vol 11 No 1 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i01.p04

Rhizoctonia solani Kuhn. is a fungal pathogen that causes sheath blight in rice plants. Sheath blight disease can reduce rice production for the people of Indonesia. To prevent blight, fungicides are used. The continuous use of synthetic fungicides with inappropriate doses can have a negative impact, so it is necessary to look for alternatives with vegetable fungicides, one of which is clove leaf (Syzygium aromaticum L.). The aim of this study was to determine the ability of clove leaf extract to inhibit the fungus R. Solani and the class of compounds contained in the extract. Data were collected and collected using the MIC (Minimum Inhibitory Concentration) test using the well-diffusion method, the inhibition percentage of clove leaf extract using the colony method, and the content of compounds using the phytochemical test. The minimum concentration (MIC) of clove leaf extract capable of inhibiting the fungus R. solani is 0.5%. The percentage of inhibitory activity can be reduced by 100% at an extract concentration of 1.5%. Clove leaf extract contains groups of alkaloids, tannins, flavonoids, saponins, phenols, steroids and terpenoids.

Co-Authors Affrina Fauziah Deni Agung Priandoko Dewa Ngurah Suprapta Fitria Dwijayanti I Gusti Ayu Diana Meirani I Gusti Ayu Laras Pratama Putri I Gusti Ayu Sugi Wahyuni I Ketut Sundra I WAYAN EKA DHARMAWAN Ida Bagus Gede Darmayasa Joko Wiryatno Made Ria Defiani Meitini Proborini Wahyuni Nasha Hadi Rahmadanty Ni Ketut Yuliana Sari Ni LUH ARPIWI Ni Luh Suriani Ni Made Rai Suarni Ni Made Suartini Nyoman Darsini Putu Anggan Pradipta Utama Rachel Daniella Dinda Maria Lumban Tobing Retno Kawuri SANG KETUT SUDIRGA Stareast Wulandari Sigiro Yulita Salma Lani

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