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All Journal Jurnal Biologi Universitas Andalas Bioeksperimen: Jurnal Penelitian Biologi Jurnal Litbang Industri Biopropal Industri Buletin Ilmiah Nagari Membangun Pelita Eksakta Bioscientist : Jurnal Ilmiah Biologi Jurnal Litbang Industri
Nurmiati, N
Andalas University
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Isolasi dan Karakterisasi Parsial Bakteri Indigenous Pemfermentasi dari Buah Durian (Durio zibethinus Murr.) Azizah Nurmalinda; - Periadnadi; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 2, No 1 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.1.%p.2013

Abstract

The research related to the isolation and partial characterization of indigenous fermenting bacteria from durian fruit (Durio zibethinus Murr.) was conducted from May to August 2012 in Laboratory of Microbiology, Biology Department, Andalas University. Experimental method was used and was descriptively analyzed. Ripe durian fruit contain indigenous fermenting as well as nonfermenting bacteria, that was recognized from halozone around the colony on modified GTA medium. Indigenous fermenting bacterias on the flesh of durian fruit have been characterized as Gram-positive, bacil, no endospores, motile and catalase positive. These isolates are proteolytics, amylolytics and cellulolytics and have no ability to hydrolyze fats and alcohol.Keywords : durian, fermenting, indigenous bacteria
Pengaruh Pengaturan Keasaman Limbah Industri Teh Terhadap Pertumbuhan Miselium Jamur Tiram Cokelat (Pleurotus cystidiosus O.K. Miller) Dalli Yullio Saputra; - Nurmiati; - Periadnadi
Jurnal Biologi Universitas Andalas Vol 2, No 4 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.4.%p.2013

Abstract

The study about the effect of acidity adjusting of industrial waste of tea production on the mycelium growth of brown oyster mushroom (Pleurotus cystidiosus O.K. Miller) was conducted from January to April 2013 in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Padang. The aim of this study is to determine the effect of setting the acidity of tea production industrial waste and the mycelium growth of brown oyster mushroom. This research was designed by completely randomized design (CRD) with five treatments, which were pH 4, pH 5, pH 6, pH 7 and pH 8 in 5 replication. The results showed that the treatment of pH 8 and pH 7 on industrial waste of tea before weathering was optimum for the growth of mycellium (18 – 19,2 days) . Keywords: Acidity, waste of tea, Pleurotus cystidiosus, mycelium.
Penapisan dan Karakterisasi Bakteri Selulolitik Termofilik Sumber Air Panas Sungai Medang, Kerinci, Jambi Uci Mela Sari; Anthoni Agustien; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 1, No 2 (2012)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.1.2.%p.2012

Abstract

A study on screening and characterization odf cellulolytic thermophylic bacteria from Sungai Medang hot spring was conducted on May to June 2012 using purposive sampling methods. This study aimed to find a cellulolytic thermophylic bacterium and to analyze its cellulolyties. Twenty eight cellulolytic thermophylic bacteria were isolated from Sungai Medang hot springs. The highest enzymatic activity (amylolytic and proteolytic activity) was shown by isolate MII2.1 from a location at which the temperature was 78oC. This isolate was characterized as an aerobic bacterium, gram negative and non motile. The form of colony was a circular margin, smooth shiny surface and elevation flat.Keywords: amylolytic, bacterium, cellulolytic, proteolytic, thermophylic
Potensi dan Karakterisasi Bakteri Alami Pencernaan Ayam Broiler Pedaging (Gallus gallus domesticus L.) Sebagai Kandidat Probiotik Pakan Ayam Broiler Adelia Febriyossa; - Nurmiati; - Periadnadi
Jurnal Biologi Universitas Andalas Vol 2, No 3 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.3.%p.2013

Abstract

A study about potential and characterization of native bacteria of intestinebroiler (Gallus gallus domesticus L.) as probiotic candidate of a broiler poultry product were conducted from December 2012 to March 2013 at Microbiology Laboratory, Department of Biology, Andalas University, Padang. This study aimed to obtainbacterial isolates and to describe morphological characters of each isolate of native bacteria as probiotic candidate for thebroiler.This study used an experimental method and analyzed descriptively. The results showed that rough material of intestine consisted of fermentative bacteria (57x107cfu/g), amylolitic (118x107cfu/g), cellulolytic (63x107cfu/g) and proteolytic bacteria (52x107cfu/g). Two isolates were potensial probioticcandidates; amylolytic bacteria and cellulolytic bacteria. The characteristics both probiotic candidates were gram negative, non-sporulating, positive catalase and motile.Keywords: Gallus gallus domesticus, intestine bacteria, characterization
Karakterisasi Mikroflora Alami Saluran Pencernaan Sapi Potong Sebagai Kandidat Probiotik Pakan Sapi Potong Uswatul Hasana; Nurmiati Nurmiati; Periadnadi Periadnadi
Jurnal Biologi Universitas Andalas Vol 2, No 4 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.4.%p.2013

Abstract

The research about the "Characterization of Natural Microflora From Cow Digestic Tract As Candidate For Cattle Feed Probiotics"  has been conducted from April to July 2013 in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Andalas, Padang. This study aimed to obtain isolates  and the character of each isolate as a candidate to cattle feed probiotics. This study used an experimental method and analyzed descriptively. The result showed that the bacteria from cow digestive tract were Cellulolytic (110x107cfu/g), Fermentative (85x107cfu/g),  Amylolytic (31x107cfu/g) and Proteolytic (28x107cfu/g) bacteria. Two of  the probiotic candidate were bacill and coccus, both of them were Gram-negative and catalase positive.
Inventarisasi Jamur Tingkat Tinggi (Basidiomycetes) di Gunung Singgalang Sumatera Barat Indra Anggriawan; - Periadnadi; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 3, No 2 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.2.%p.2014

Abstract

The macrofungi of Basidiomycetes was collected in Singgalang mountain and then identified in Microbiology Laboratory, Department of Biology, Faculty of Sciences, Andalas University. The aim of this study was to identify the macrofungi from Basidiomycetes. This study used direct survey method to collect macrofungi from the field. This study identified 22 genera which belong to 10 family and 6 orders. Most of the fungi were belong to subclass of Hymenomycetidae.Keywords: basidiomycetes, inventories, macrofungi.
Pengaruh Penambahan Ekstrak Daun dan Bubuk Teh, Kopi, dan Coklat Terhadap Fermentasi Nata De Coco Merry Tri Indah Permata Sari; - Periadnadi; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 3, No 3 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.3.%p.2014

Abstract

This study aimed of to find out the effect of leaf extract and seed powder containing alkaloid which occured on the production process of nata de coco. An experiment was conducted using Complete Randomized Design (CRD) with factorial pattern. The result showed that the brewed extract of tea, coffee, chocolate affected the weight product of nata de coco. The brewed extract of green tea showed the best result on weight of the nata (24.66 g) which has a significant difference from the treatments of black tea, coffee powder, cocoa powder, coffee leaf, and chocolate leaf. The best dose of brewed extract in obtaining nata was 2 g/100 ml.Keywords : alkaloid, nata de coco, effect.
Isolasi Bakteri Pendegradasi Hidrokarbon di Tanah Tercemar Lokasi Perbengkelan Otomotif Hezi Yolantika; Periadnadi Periadnadi; Nurmiati Nurmiati
Jurnal Biologi Universitas Andalas Vol 4, No 3 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.3.%p.2015

Abstract

The research related to Isolation of the Hydrocarbon Degrading Bacteria in the Contaminated Soil of the Automotive Workshop was conducted from May to October 2014 at Laboratory of Microbiology, Biology Department, Faculty of Mathematics and Natural Sciences, Andalas University. The aimed of this study was to find  the bacteria able to degradate the hydrocarbon from the lubricating oil contaminated soil and to descripe characterization of isolates. This study used a survey method in isolation and characterization of hydrocarbon degrading bacteria.  The result obtained that the bacteria able to degradate the hydrocarbon from the lubricating oil contaminated soil about 39.02% (1.6x105 cfu/g). Five isolates were found (BTTO1, BTTO2, BTTO3, BTTO4 and BTTO5) has a rod shaped, Gram-negative, motile and positive catalase.
ISOLASI DAN KEBERADAAN KHAMIR POTENSIAL PEMFERMENTASI NIRA AREN (Arenga pinnata Merr.) DARI DATARAN RENDAH DAN DATARAN TINGGI DI SUMATERA BARAT Periadnadi Periadnadi; Diah Kharisma Sari; Nurmiati Nurmiati
Bioeksperimen: Jurnal Penelitian Biologi Vol 4, No 1: March 2018
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v4i1.5927

Abstract

Penelitian bertujuan untuk mengisolasi dan mengkarakter khamir potensial pemfermentasi nira aren segar dari dataran rendah dan dataran tinggi di Sumatera Barat. Penelitian menggunakan metode eksperimen yang hasilnya disajikan secara deskriptif. Parameter yang diamati meliputi total mikroba, total khamir, uji potensi isolat khamir melalui uji fermentasi, karakter morfologi isolat khamir potensial pemfermentasi secara in vitro, serta analisis biokimiawi nira aren. Hasil penelitian menunjukkan bahwa keberadaan mikroba alami nira aren tertinggi terdapat pada sampel PAM1 (168 x 104 cfu/ml), total khamir tertinggi terdapat pada sampel HLB (85 x 104 cfu/ml), sedangkan presentase khamir tertinggi terdapat pada sampel HLB (69,1%). Uji potensi isolat melalui uji fermentasi diperoleh 5 isolat yang positifmemfermentasi alkohol. Berdasarkan karakter morfologi isolat-isolat khamir dari beberapa lokasi sampel secara in vitro didapatkan 3 isolat genus Hanseniaspora, 1 isolat genus Schizosaccharomyces, dan 1 isolat genus Saccharomyces.
Pengaruh jenis isolat-isolat bakteri fermentatif dari ubi kayu terhadap rendemen, derajat putih, dan bentuk granula tepung mocaf K Kamsina; N Nurmiati; P Periadnadi
Jurnal Litbang Industri Vol 9, No 2 (2019)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24960/jli.v9i2.5651.135-140

Abstract

Isolat bakteri indigenous ubi kayu merupakan isolat bakteri asli yang berada dalam umbi ubi kayu. Ubi kayu jenis lambau dan ketan (Manihot esculenta Crantz) merupakan jenis ubi kayu lokal Sumatera Barat, sedangkan ubi kayu kultivar karet (Manihot glaziovii)  merupakan ubi kayu dengan kandungan asam sianida (HCN) tinggi. Penelitian ini bertujuan untuk melihat kemampuan masing-masing isolat bakteri indigenous ubi kayu terhadap rendemen, derajat putih serta bentuk granula tepung mocaf yang dibandingkan dengan perlakuan tanpa pemberian isolat. Penelitian menggunakan rancangan acak lengkap (RAL) dengan perlakuan pemberian starter isolat bakteri indigenous ubi kayu jenis ketan (A), lambau (B), karet (C) dan tanpa isolat/kontrol (D). Terhadap produk mocaf  yang dihasilkan dilakukan pengujian meliputi rendemen, derajat putih, dan bentuk granula tepung mocaf. Hasil analisis terhadap berbagai perlakuan menunjukkan perlakuan optimal untuk tepung mocaf yang dihasilkan adalah pemberian starter isolat bakteri indigenous jenis ketan dengan rendemen  35,85%, derajat putih 94,40% dengan dinding sel ubi kayu sudah banyak yang pecah menjadi granula-granula yang lebih kecil dan memiliki rongga pada bagian granulanya.ABSTRACTCassava indigenous bacterial isolates are native bacterial isolates that are in tubers of cassava. Lambau and sticky rice (Manihot esculenta Crantz) are types of local cassava from West Sumatra, while rubber cultivar cassava (Manihot glaziovii) is a cassava with a high content of cyanide acid (HCN). This study was aimed to look at the ability of each indigenous cassava isolate to yield, the degree of white and the form of mocaf flour granules compared to the treatment without administration of isolates. The study used a completely randomized design (CRD) with the following treatments: administration of indigenous cassava isolate starter of sticky rice type (A), lambau (B), rubber (C) and without isolate / control (D). The mocaf products were tested including yields, white degrees, and the granules shape of mocaf flour. The analysis results of various treatments showed that the optimal treatment for mocaf flour produced was the provision of indigenous bacterial isolates starter from sticky rice type (A) with a yield of 35.85%,  white degree 94.40% with a lot of cassava cell walls that had broken into smaller granules and had cavities on the granules.
Co-Authors Agustian Agustian Andi Alatas, Andi Ando Fernando Annisa Apriyelita Anthoni Agustien Awari Susanti Ayu Putri Ningsih Azizah Nurmalinda Dalli Yulio Saputra Dalli Yullio Saputra Deddi Prima Putra Diah Kharisma Sari Efrizal Efrizal Fevria, Resti Hafizatur Rahma Hermansah Hermansah Hezi Yolantika Indra Anggriawan K Kamsina Kamsina, K M. Fiqri Fadhlan Maiyontoni Maiyontoni Merla Pamericar Merry Tri Indah Permata Sari Mitra Angelia Nadia Merisya Ossy Y. Prastika Periadnadi Periadnadi Periadnadi Periadnadi Putri, Santi Diana Rahmadani, Silmi Yusri Rahmi Adila Ramza Seswati Refa Rahmaddiansyah Risca Adelina Atro Sherly Jadespi Sri Indrayati Sukarsi Rusti Tesri Maideliza Tivani Ardini Uci Mela Sari Uswatul Hasana Wira Hastuti Yulia M. Nur