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Isolasi dan Karakterisasi Parsial Bakteri Indigenous Pemfermentasi dari Buah Durian (Durio zibethinus Murr.) Azizah Nurmalinda; - Periadnadi; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 2, No 1 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.1.%p.2013

Abstract

The research related to the isolation and partial characterization of indigenous fermenting bacteria from durian fruit (Durio zibethinus Murr.) was conducted from May to August 2012 in Laboratory of Microbiology, Biology Department, Andalas University. Experimental method was used and was descriptively analyzed. Ripe durian fruit contain indigenous fermenting as well as nonfermenting bacteria, that was recognized from halozone around the colony on modified GTA medium. Indigenous fermenting bacterias on the flesh of durian fruit have been characterized as Gram-positive, bacil, no endospores, motile and catalase positive. These isolates are proteolytics, amylolytics and cellulolytics and have no ability to hydrolyze fats and alcohol.Keywords : durian, fermenting, indigenous bacteria
Pengaruh Pengaturan Keasaman Limbah Industri Teh Terhadap Pertumbuhan Miselium Jamur Tiram Cokelat (Pleurotus cystidiosus O.K. Miller) Dalli Yullio Saputra; - Nurmiati; - Periadnadi
Jurnal Biologi Universitas Andalas Vol 2, No 4 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.4.%p.2013

Abstract

The study about the effect of acidity adjusting of industrial waste of tea production on the mycelium growth of brown oyster mushroom (Pleurotus cystidiosus O.K. Miller) was conducted from January to April 2013 in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Padang. The aim of this study is to determine the effect of setting the acidity of tea production industrial waste and the mycelium growth of brown oyster mushroom. This research was designed by completely randomized design (CRD) with five treatments, which were pH 4, pH 5, pH 6, pH 7 and pH 8 in 5 replication. The results showed that the treatment of pH 8 and pH 7 on industrial waste of tea before weathering was optimum for the growth of mycellium (18 – 19,2 days) . Keywords: Acidity, waste of tea, Pleurotus cystidiosus, mycelium.
Penapisan dan Karakterisasi Bakteri Selulolitik Termofilik Sumber Air Panas Sungai Medang, Kerinci, Jambi Uci Mela Sari; Anthoni Agustien; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 1, No 2 (2012)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.1.2.%p.2012

Abstract

A study on screening and characterization odf cellulolytic thermophylic bacteria from Sungai Medang hot spring was conducted on May to June 2012 using purposive sampling methods. This study aimed to find a cellulolytic thermophylic bacterium and to analyze its cellulolyties. Twenty eight cellulolytic thermophylic bacteria were isolated from Sungai Medang hot springs. The highest enzymatic activity (amylolytic and proteolytic activity) was shown by isolate MII2.1 from a location at which the temperature was 78oC. This isolate was characterized as an aerobic bacterium, gram negative and non motile. The form of colony was a circular margin, smooth shiny surface and elevation flat.Keywords: amylolytic, bacterium, cellulolytic, proteolytic, thermophylic
Potensi dan Karakterisasi Bakteri Alami Pencernaan Ayam Broiler Pedaging (Gallus gallus domesticus L.) Sebagai Kandidat Probiotik Pakan Ayam Broiler Adelia Febriyossa; - Nurmiati; - Periadnadi
Jurnal Biologi Universitas Andalas Vol 2, No 3 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.3.%p.2013

Abstract

A study about potential and characterization of native bacteria of intestinebroiler (Gallus gallus domesticus L.) as probiotic candidate of a broiler poultry product were conducted from December 2012 to March 2013 at Microbiology Laboratory, Department of Biology, Andalas University, Padang. This study aimed to obtainbacterial isolates and to describe morphological characters of each isolate of native bacteria as probiotic candidate for thebroiler.This study used an experimental method and analyzed descriptively. The results showed that rough material of intestine consisted of fermentative bacteria (57x107cfu/g), amylolitic (118x107cfu/g), cellulolytic (63x107cfu/g) and proteolytic bacteria (52x107cfu/g). Two isolates were potensial probioticcandidates; amylolytic bacteria and cellulolytic bacteria. The characteristics both probiotic candidates were gram negative, non-sporulating, positive catalase and motile.Keywords: Gallus gallus domesticus, intestine bacteria, characterization
Karakterisasi Mikroflora Alami Saluran Pencernaan Sapi Potong Sebagai Kandidat Probiotik Pakan Sapi Potong Uswatul Hasana; Nurmiati Nurmiati; Periadnadi Periadnadi
Jurnal Biologi Universitas Andalas Vol 2, No 4 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.4.%p.2013

Abstract

The research about the "Characterization of Natural Microflora From Cow Digestic Tract As Candidate For Cattle Feed Probiotics"  has been conducted from April to July 2013 in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Andalas, Padang. This study aimed to obtain isolates  and the character of each isolate as a candidate to cattle feed probiotics. This study used an experimental method and analyzed descriptively. The result showed that the bacteria from cow digestive tract were Cellulolytic (110x107cfu/g), Fermentative (85x107cfu/g),  Amylolytic (31x107cfu/g) and Proteolytic (28x107cfu/g) bacteria. Two of  the probiotic candidate were bacill and coccus, both of them were Gram-negative and catalase positive.
Inventarisasi Jamur Tingkat Tinggi (Basidiomycetes) di Gunung Singgalang Sumatera Barat Indra Anggriawan; - Periadnadi; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 3, No 2 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.2.%p.2014

Abstract

The macrofungi of Basidiomycetes was collected in Singgalang mountain and then identified in Microbiology Laboratory, Department of Biology, Faculty of Sciences, Andalas University. The aim of this study was to identify the macrofungi from Basidiomycetes. This study used direct survey method to collect macrofungi from the field. This study identified 22 genera which belong to 10 family and 6 orders. Most of the fungi were belong to subclass of Hymenomycetidae.Keywords: basidiomycetes, inventories, macrofungi.
Pengaruh Penambahan Ekstrak Daun dan Bubuk Teh, Kopi, dan Coklat Terhadap Fermentasi Nata De Coco Merry Tri Indah Permata Sari; - Periadnadi; - Nurmiati
Jurnal Biologi Universitas Andalas Vol 3, No 3 (2014)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.3.3.%p.2014

Abstract

This study aimed of to find out the effect of leaf extract and seed powder containing alkaloid which occured on the production process of nata de coco. An experiment was conducted using Complete Randomized Design (CRD) with factorial pattern. The result showed that the brewed extract of tea, coffee, chocolate affected the weight product of nata de coco. The brewed extract of green tea showed the best result on weight of the nata (24.66 g) which has a significant difference from the treatments of black tea, coffee powder, cocoa powder, coffee leaf, and chocolate leaf. The best dose of brewed extract in obtaining nata was 2 g/100 ml.Keywords : alkaloid, nata de coco, effect.
Isolasi Bakteri Pendegradasi Hidrokarbon di Tanah Tercemar Lokasi Perbengkelan Otomotif Hezi Yolantika; Periadnadi Periadnadi; Nurmiati Nurmiati
Jurnal Biologi Universitas Andalas Vol 4, No 3 (2015)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.4.3.%p.2015

Abstract

The research related to Isolation of the Hydrocarbon Degrading Bacteria in the Contaminated Soil of the Automotive Workshop was conducted from May to October 2014 at Laboratory of Microbiology, Biology Department, Faculty of Mathematics and Natural Sciences, Andalas University. The aimed of this study was to find  the bacteria able to degradate the hydrocarbon from the lubricating oil contaminated soil and to descripe characterization of isolates. This study used a survey method in isolation and characterization of hydrocarbon degrading bacteria.  The result obtained that the bacteria able to degradate the hydrocarbon from the lubricating oil contaminated soil about 39.02% (1.6x105 cfu/g). Five isolates were found (BTTO1, BTTO2, BTTO3, BTTO4 and BTTO5) has a rod shaped, Gram-negative, motile and positive catalase.
ISOLASI DAN KARAKTERISASI BAKTERI ALAMI PENCERNAAN IKAN PATIN SIAM (Pangasius hypophthalmus ) SEBAGAI KANDIDAT PROBIOTIK Awari Susanti; Periadnadi Periadnadi; Nurmiati Nurmiati
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p17

Abstract

Penelitian tentang Isolasi dan Karakterisasi Bakteri Alami Pencernaan Ikan Patin Siam (Pangasius hypophthalmus) Sebagai Kandidat Probiotik di laksanakan di Laboratorium Mikrobiologi, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Andalas, dari bulan Oktober 2015 sampai Mei 2016. Penelitian ini dilakukan dengan metoda ekperimen terhadap karakter mikroflora alami pencernaan ikan patin yang di analisis secara deskriftif. Bakteri indigenous di isolasi dengan medium GPA + CaCo3, isolat yang didapat di tentukan karakter dan potensi nya. Hasil Penelitian menunjukkan bahwa didalam usus Ikan Patin Siam pada medium GPA + CaCo3 ditemukan 32 x 106 cfu/g bakteri indigenous yang bersifat fermentatif, yang dapat mengubah gula menjadi asam. Keempat isolat yang didapatkan memiliki karakteristik garam positif dan gram negatif. Berbentuk batang, dengan bentuk koloni yang berbeda-beda, katalase yang bereaksi positif dan negatif serta motilitas yang positif. Pada isolat PIRI3 memiliki nilai indeks degradatif tertinggi 1,6 dan tergolong dalam bakteri asam laktat pada genus Lactobacillus.
Keberadaan Mikroba Pemfermentasi pada Minuman Kefir Air Susu Kambing Etawa Merla Pamericar; Periadnadi Periadnadi; Nurmiati Nurmiati
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p15

Abstract

Study about existence of fermentation microbe in kefir etawa goat milk had been studied. The objective of this study were to know total of microbe from kefir etawa goat milk. This study used descriptive method and counting population of microbe on in vitro. The variable acid in kefir measuring with pH meter. Result indicated total bacteria in kefir etawa goat milk are sebanyak 98,3 x 106 cfu/ml with total yeast 67,8 x 105 cfu/ml and pH of kefir 4.57.