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Kualitas Air, Pertumbuhan dan Kelulushidupan Benih Abalone (Haliotis squamata) dengan Aplikasi RAS di BPIUUK Karangasem Bali Arief Prayoga Akbar; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Bumi Lestari Journal of Environment Vol 22 No 2 (2022)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2022.v22.i02.p01

Abstract

Abalone is one of the sea snails that have high economic value and has advantages because it has a high protein content of 71.99%, a fat content of 3.24%, and can be used as medicine. The high demand for abalone causes the increase of wild capture and lead to scarcity of abalone in the waters. One way to overcome this problem is by cultivate the abalone. Abalone cultivation can be done with a monoculture system. One of the technologies applied is by using a recirculation aquaculture system (RAS), which is reprocessing the water used so that it can be reused for cultivation activities. The purpose of this study was to analyze the parameters of water quality, growth length, weight and survival of abalone (Haliotis squamata) seeds using the RAS system. The research was conducted in June – September 2020 at the Shrimp and Shellfish Parent Center, Karangasem, Bali. The research was performed by purposive sampling method. The water quality parameters data such as nitrite, ammonia, TSS pH, temperature, DO, and salinity were taken. The results of the measurement of water quality are nitrite 0 – 0.338 mg/L, ammonia 0 – 0.09 mg/L, TSS 0 – 15 mg/L, pH 7 – 8, temperature 26 – 28 oC, DO 4.3 – 7.07 mg/L, and salinity 31 – 38 ppt. Those values are still in the optimal range for abalone culture. The growth in length and absolute weight of abalone seeds during the study were 25.17 mm/ind. and 9.15 g/ind., respectively, and the survival value was 99.5% during the 100-day culture period.
THE EFFECT OF Bacillus sp. ADDITION ON THE VIRULENCE OF Vibrio parahemolyticus TOWARD AXENIC CULTURE OF Artemia franciscana Ni Luh Komang Ayu Maitri Jayanthi; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Journal of Fish Health Vol. 2 No. 1 (2022): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.479 KB) | DOI: 10.29303/jfh.v2i1.1389

Abstract

Research on the benefits of Bacillus sp. in aquaculture has been done a lot. Currently, the aquaculture industry, especially shrimp farming, is facing AHPND disease, which is known to be caused by Vibrio parahaemolyticus. This study aims to see the effect of Bacillus sp. against virulence of V. parahaemolyticus in vitro using caseinase production as an indicator and survival of Artemia franciscana cultured axenically when challenged with V. parahaemolyticus. In in vitro tests, cultures of V. parahaemolyticus were grown with and without the addition of Bacillus sp. on Luria Bertani (LB) liquid media, and then the caseinase test was carried out using skim milk (SM) agar as a growth medium. In the challenge test, 10 axenic cultured Artemia sp. were distributed in falcon tubes containing 10 ml of sterile seawater. Challenge test treatments consisted of treatment A as control of Artemia culture without the addition of bacteria, treatment B Artemia culture adding by 106 CFU/ml V. parahaemolyticus, treatment C Artemia culture with the addition of 106 CFU/ml Bacillus sp., and treatment D Artemia culture with the addition of 106 CFU/ml V. parahaemolyticus and 106 CFU/ml Bacillus sp. All treatments were done in triplicates. The results showed that the addition of Bacillus sp. was able to reduce the caseinase production of V. parahemolyticus up to 29% indicated by the decrease of the clearing zone diameter formed on SM agar. Furthermore, the addition of Bacillus sp. in treatment D was able to significantly increasing (P<0.05) of Artemia's survival when challenged with V. parahamolyticus. This indicates that Bacillus sp. has a potential as a probiotic candidate to prevent disease caused by V. parahaemolyticus.
Strategi Pengembangan Investasi Di Pelabuhan Perikanan Nusantara (PPN) Pengambengan, Bali I Ketut Wija Negara; Dewa Ayu Angga Pebriani; Ni Putu Putri Wijayanti; Andi Mannojengi; Bagus Sudananjaya; Kadek Adi Chandra Kusuma
Journal of Marine and Aquatic Sciences Vol 8 No 1 (2022)
Publisher : Fakultas Kelautan dan Perikanan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/jmas.2022.v08.i01.p02

Abstract

The construction of a fishing port has been able to have an impact on growth in the economic sector in the fishery sector, economic growth which can directly improve the welfare of the fishing community. The purpose of this research is to be able to plan a strategy that must be implemented by the manager of the PPN Pengambengan to encourage the business development of service users in the PPN Pengambengan. The benefits obtained from this research are, to be able to improve the function of the PPN Pengambengan in serving the needs of coastal communities, fisher, the fishing industry, and other agencies so that they can directly develop the potential of resources around the port area both socially and economically. Data analysis in this research uses SWOT analysis with the stages of determining IFAS and EFAS, Matrix, SWOT Quadrants. The results of the study get an IFAS value of 0.34 (x), and an EFAS value of – 0.02 (y), where the value is in the Diversification Strategy position or using the ST strategy. The strategies offered for investment development in PPN Pengambengen are in the Diversification Strategy (ST) quadrant position, namely; transfer of fishing technology, controlling the loading and unloading process through improving K5 performance, special land for SPBN refueling, and expansion of road infrastructure for the fisheries industry to be adequate for the mobilization.
Respon Imun Ikan Nila (Oreochromis niloticus) Yang Terinfeksi Bakteri (Aeromonas hydrophila) Dengan Penambahan Vitamin C Pada Pakan Dewa Ayu Angga Pebriani; Ni Putu Putri Wijayanti; Putu Eka Sudaryatma; Octovianus Octovianus
Journal of Marine and Aquatic Sciences Vol 8 No 2 (2022)
Publisher : Fakultas Kelautan dan Perikanan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/jmas.2022.v08.i02.p01

Abstract

Tilapia (Oreochromis niloticus) is one of the freshwater fish commodities with important economic value. However, in tilapia aquaculture, the emergence of disease was one of the problems that affect the quality and quantity of aquaculture production. One of the diseases that often appears was infection with Aeromonas hydrophila bacteria. This problem is expected to be overcome by giving vitamin C in fish feed. Through the provision of vitamin C, it will be able to increase the fish's immune system which is directly related to the fish's ability to prevent or treat disease attacks that arise, especially from Aeromonas hydrophila bacteria. The aim of this study was to determine the response of vitamin C to the immune system of fish infected with Aeromonas hydrophila so that the best dose was known that could be applied in the treatment of Aeromonas hydrophyla bacteria attack. The study was conducted using an experimental method with a completely randomized design (CRD) consisted of 2 controls, namely positive control and negative control, and 3 treatments with different doses of vitamin C. Based on the hematological test, the results showed that the content of white blood cells, red blood cells, and hemoglobin were still in the normal range for all treatments. Treatment E with a dose of 450 mg/kg vitamin C was able to increase the immune system of tilapia infected with Aeromonas hydrophila bacteria, shown from the content of white blood cells, red blood cells and hemoglobin in the highest treatment E compared to other treatments.
Kandungan Nitrat dan Fosfat pada Air Sungai Yeh Ho Tabanan, Bali Lasmana Prasat; Ima Yudha Perwira; Dewa Ayu Angga Pebriani
Current Trends in Aquatic Science Vol 5 No 2 (2022)
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Kelautan dan Perikanan, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aimed to determine the value of nitrate, phosphate, and the quality state of the water in the Yeh Ho river Tabanan Regency Bali based on the Government Regulation Number 22/2021. This research was conducted at 3 different location (upstream as station 1, middle stream as station 1, and downstream as station 3) of the Yeh Ho River (Tabanan, Bali) during February 2021. Water sample was analyzed for nitrate, phosphate, and the other water quality parameters (pH, temperature, and DO). Nitrate was measured using KIT Salifert Nitrate, phosphate was measured using KIT Hanna HI713, pH was measured using pH meter, water temperature was measured using thermometer, and DO was measured using DO meter. The result showed that nitrate in the water of Yeh Ho river was ranging from 0.25 to 0.29 mg/L, while phosphate was ranging from 0.10 to0.19 mg/L. The average value of pH, temperature, and DO in the water were: 6,95 mg/L, 27,15oC, and 5,2 mg/L, respectively. This result indicates that nitrate and phosphate value in the water of Yeh Ho river is belong to Class II and III which can be used for aquaculture and recreational use.
Distribusi Mikrohabitat dan Infeksi Endoparasit pada Ikan Kembung (Rastrelliger sp.) yang didaratkan di PPI Kedonganan, Badung, Bali Claudy Margaretha Kandouw; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Journal of Marine and Aquatic Sciences Vol 9 No 1 (2023)
Publisher : Fakultas Kelautan dan Perikanan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/jmas.2023.v09.i01.p13

Abstract

Mackerel (Rastrelliger sp.) is a pelagic fish commodity that has high economic value because mackerel is a consumption fish and is easily found in all sea waters in Indonesia. Mackerel is a fish that is hunted for consumption because of its high omega-3 content, so it is really necessary for us to pay attention to the quality of the mackerel from the threat of parasites that can cause disease. This research was being conducted to determine the distribution of endoparasite microhabitats, intensity, prevalence, and the relation between the length of the fish and weight of fish with the number of parasites. The research samples were collected from Kedonganan Fish Landing Base, Badung, Bali, which was carried out from October 2021 to November 2021. The research used a descriptive quantitative method and the sampling method was carried out by random sampling. The total samples of fish examined were 35 fish consisting of two species, R. Kanagurta (20 fish) and R. brachysoma (15 fish). Endoparasites of the Anisakis genus were parasites with the highest intensity and prevalence values : 4,06 ind/fish and 82,86% with a low infection rate. The relation between the length of the fish and body weight of fish with the number of parasites was in the opposite direction and had a very low effect with a value of correlation (r) between length and the number of parasites are 0,031 and a value of correlation (r) between weight and number of parasites are 0,00547.
Kelimpahan Vibrio sp. dan Total Bakteri Pada Sampel Air Tambak Udang Vaname yang Terindikasi Terjangkit Infectious Myonecrosis Virus (IMNV) Grace Adelina Girsang; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Jurnal Ilmu Manajemen, Bisnis dan Ekonomi Vol 3 No 3 (2025)
Publisher : PT Maju Malaqbi Makkarana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59971/jimbe.v3i3.689

Abstract

Tambak udang vaname (Litopenaeus vannamei) banyak ditemukan di Indonesia dan membutuhkan pengelolaan kualitas air yang baik untuk mendukung pertumbuhan dan kesehatan udang. Meskipun udang vaname relatif tahan terhadap beberapa penyakit, ia tetap rentan terhadap infeksi bakteri Vibrio dan virus Infectious Myonecrosis Virus (IMNV), yang dapat menurunkan kualitas produksi udang akibat kerusakan otot dan infeksi sekunder. Kelimpahan Vibrio mengacu pada jumlah bakteri dari genus Vibrio dalam air, yang dapat memperburuk kesehatan udang, terutama jika dipengaruhi oleh faktor lingkungan seperti suhu, pH, dan kualitas air yang buruk. Sementara itu, total bakteri mengacu pada jumlah keseluruhan bakteri dalam sampel air, baik yang menguntungkan maupun patogen, yang penting untuk menilai kualitas ekosistem tambak dan potensi risiko kesehatan bagi udang. Penelitian ini bertujuan untuk mengetahui kelimpahan bakteri Vibrio dan total bakteri pada air tambak udang vaname yang terindikasi terjangkit IMNV. Penelitian ini menggunakan metode deskriptif kuantitatif dengan teknik kultur mikrobiologi untuk menghitung kelimpahan Vibrio dan total bakteri. Sampel air diambil dari tambak yang terindikasi terjangkit IMNV di Bali, dengan analisis parameter kualitas air seperti salinitas, suhu, pH, DO, nitrit, dan amonia. Hasil penelitian menunjukkan kelimpahan Vibrio rata-rata 2 x 10⁴ cfu/mL dan total bakteri 3.17 x 10⁶ cfu/mL. Kualitas air tambak tersebut menunjukkan kondisi yang mendukung pertumbuhan bakteri, dengan nilai TSS dan amonia yang tinggi meningkatkan risiko bakteri patogen.
Uji Daya Hambat Ekstrak Bawang Putih terhadap Vibrio sp. dari Tambak Udang Vannamei yang Terkena White Feces Disease (WFD) Budhi Wahyu Nurindra; Pande Gde Sasmita Julyantoro; Dewa Ayu Angga Pebriani
Manfish: Jurnal Ilmiah Perikanan dan Peternakan Vol. 3 No. 2 (2025): September: Manfish: Jurnal Ilmiah Perikanan dan Peternakan
Publisher : Asosiasi Riset Ilmu Tanaman Dan Hewani Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62951/manfish.v3i2.259

Abstract

White Feces Disease (WFD), which infects Pacific white shrimp (Litopenaeus vannamei), poses a serious challenge in aquaculture due to its potential to cause mass mortality and substantial economic losses. One of the main causes of this disease is bacterial infection from the genus Vibrio. The use of natural substances, such as garlic (Allium sativum) extract, is considered a more environmentally friendly and sustainable alternative for disease control. This study aimed to examine the effect and determine the optimal concentration of garlic extract in inhibiting the growth of Vibrio sp. isolated from WFD-infected L. vannamei ponds, cultured on TCBS media. The study was conducted in vitro using the disc diffusion method with four extract concentrations (25%, 50%, 75%, and 100%) against two bacterial isolates, designated as isolate A and isolate B. The results showed that garlic extract was able to inhibit the growth of Vibrio sp., with the largest inhibition zone observed in isolate A at 75% concentration (14.8 mm) and in isolate B at 100% concentration (19.3 mm). These findings indicate the potential of garlic as a natural antibacterial agent to inhibit pathogenic bacteria and support the sustainability of shrimp aquaculture.
Effect of vitamin C supplementation on the survival rate and histopathological changes of gills and kidneys of tilapia (Oreochromis niloticus) infected by Aeromonas hydrophylla Dewa Ayu Angga Pebriani; I Ketut Wija Negara; Ni Putu Putri Wijayanti; Sudaryatma, Putu Eka; Putu Angga Wiradana
Journal of Aquaculture and Fish Health Vol. 13 No. 2 (2024): JAFH Vol. 13 No. 2 June 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i2.43897

Abstract

This study was conducted to determine the effect of vitamin C supplementation on the survival rate and histopathological changes of gills and kidneys of tilapia infected by Aeromonas hydrophila. Three doses of vitamin C were tested (150; 300; and 450 mg/kg) with two control groups. Tilapia with uniform size (average weight of 14 grams) as the criteria for inclusion were randomly distributed in five ponds with 15 tilapia fish per ponds. Tilapia was fed with hands until full for two weeks. Tilapia was then infected with A. hydrophila to find out the survival rate and histopathological changes of gills and kidneys at the end of the experimental period or 7 days after infection. The supplementation of 150 mg/kg vitamin C in feed increased the survival rate (%) in Tilapia by 86.67% or higher than other treatment and control groups. The damage to gills in terms of lamella separation was found in all treatment groups, as well as kidneys. The results found that the supplementation of 150 mg/kg vitamin C in feed increased survival rate but did not give effective protection on gills and kidneys.
Echinoderms Community Structure In Pegametan Bay, Buleleng, Bali Arya Maulana Rabani; Dewa Ayu Angga Pebriani; I Ketut Wija Negara
Advances in Tropical Biodiversity and Environmental Sciences Vol. 8 No. 3 (2024): October 2024: Pages 119-170
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2024.v08.i03.p07

Abstract

Echinoderms are key ecological organisms that play a protective role in balancing the marine ecosystem by breaking down trash or leftover organic material that is not used by other species. Echinoderms are marine biota that are sensitive to changes in water characteristics, so they can be used as bioindicators. Echinoderm habitats can be found in all marine ecosystems, but the highest diversity is found in intertidal areas, which are easily affected by land activity. One of the intertidal areas that is often used by humans is the Bay Area. This research aims to find out the types of echinoderms and analyze the community structure of echinoderms in Pegametan Bay, Bali. The research was carried out using the method of line transect at 18 points for data on the number and types of echinoderms in depth 1x1 m quadrat transect. Based on the observations, it was found that there are 3 classes of echinoderms that live on Bingin Beach (Echinoidea, Asteroidea, and Holothuroidea) with 3 orders, namely Diadematoida, Valvatida, and Holothuriida. The most commonly found species is D. setosum from the Echinoidea class. The structure of the echinoderm community in Pegametan Bay is shown by an average density value of 12.60 ind/m² with a diversity index value of 0.46, which is classified as low diversity; a uniformity index value of 0.36, which is relatively low; and a dominance index of 0.73, which is relatively high. Based on the results obtained, it can be concluded that the diversity index in Pegametan Bay is low, the evenness index is relatively low, and the dominance index is high. The values of temperature, pH, dissolved oxygen (DO), and salinity are still within the standard limits for marine biota.