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Exploring Microbial Diversity in Green Honey from Pulau Banggi Sabah: A Preliminary Study: Microbial Diversity in Green Honey from Pulau Banggi Sabah Rajindran, Nanthini; Ab Wahabb, Roswanira; Huda , Nurul; Adekilekun Oyewusi, Habeebat; Wayan Gunam, Ida Bagus; Mohd Shariff , Amir Husni; Izzah Ismail, Norjihada; Huyop, Fahrul
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.02

Abstract

The microbiological composition of honey can include microorganisms that are beneficial or harmful to human health. Therefore, it is essential to investigate the microbiological quality of different honey types available in the market. However, there is limited information available on the analysis, isolation, and characterization of honey-associated microbes, especially for green honey from Banggi Island. Green honey is sourced from underground areas within the island's forest. This study aimed to assess the microbiological quality of raw (freshly collected) and processed green honey by examining the presence of bacteria, yeast, molds, and pathogens. The results revealed that raw green honey had a slightly higher total plate count (770 ± 0.03 cfu/g) compared to processed green honey (640 ± 0.02 cfu/g). Both raw and processed green honey contained Lactobacillus spp. with counts of 350 ± 0.02 cfu/g and 160 ± 0.02 cfu/g, respectively. Bacillus count was higher in raw green honey (110 ± 0.01 cfu/g) compared to processed green honey (5 ± 0.01 cfu/g). Molds were only detected in raw green honey, while osmophilic yeast counts were higher in raw green honey (16000 ± 0.03 cfu/g) compared to processed green honey (120 ± 0.02 cfu/g). Mesophilic bacteria, thermophilic bacteria, coliforms, E. coli, and Staphylococcus aureus were not detected in either raw or processed green honey. Furthermore, green honey was free from pathogenic bacteria such as Salmonella spp., Listeria spp., and Shigella spp. Bacteria isolated from green honey included Lysinibacillus macrolides, Lysinibacillus boronitolerans, Paenibacillus cineris, Paenibacillus favisporus, and Bacillus oleronius, none of which were pathogenic. This study identified important microorganisms present in green honey, which have the potential to provide beneficial effects without posing any harm to human health.
In silico Characterization of Poly (ethylene) Terephthalate (PET): Degrading Enzymes from Rhizobacter sp. for Enzymatic Degradation Mechanisms: Characterization of Rhizobacter sp. PET Hydrolases Damuri, Nur Wahida; Mohd Rozdhi, Amira Azawani; Tirmizhi Abubakar, Munkaila; Wayan Gunam, Ida Bagus; Huyop, Fahrul; Oyewusi, Habeebat Adekilekun
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Dienelactone hydrolase (DHL) from Rhizobacter sp. is an enzyme from the β‐ketoadipate pathway that belongs to the α/β hydrolase family. It involves the conversion of chloroaromatics, such as nitrophenols and hydrocarbons, into harmless metabolites. The sequence-based analysis of Dienelactone hydrolase from Rhizobacter sp. shows significant homology to the extensively studied polyethylene terephthalate hydrolase of Ideonella sakaiensis (IsPETase). IsPETase can degrade the polymer, polyethylene terephthalate (PET), at room temperature. It was chosen as a template for dienelactone hydrolase from Rhizobacter sp. that was studied as a putative PET hydrolase. This study employs bioinformatics tools such as Expasy Protparam, Clustal Omega, SWISS-MODEL, GROMACS and Autodock vina to analyse the amino acid sequence of this enzyme, predict its three-dimensional structure and study its binding interaction. The structure of the putative PET hydrolase has been determined with 0.9 GMQE value and an overall quality factor of 96%. The residues responsible in substrate binding interactions are Leu88, Ser160 and Trp185. Thus, this in silico analysis depicts the ability of the putative PET hydrolase to bind to the polymer polyethylene terephthalate.
Structural Comparison of α-agarase (α-AgaD) from Thalassomonas sp. LD5: An in-silico study: Structural Comparison of α-agarase (α-AgaD) from Thalassomonas sp. LD5 Oyewusi, Habeebat Adekilekun; Oladipo, Oluwatosin; Abdul Wahab, Roswanira; Adekilekun , Habeebulahi Ajibola; Wayan Gunam, Ida Bagus; Huyop, Fahrul
Journal of Tropical Life Science Vol. 15 No. 2
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

The significance of agarase enzymes spans various high-value industries, including food, cosmetics, and medicine. These enzymes play a crucial role in the hydrolysis of agar to produce bioactive oligosaccharides, enabling wide-ranging applications across multiple fields. Among them, α-AgaD is a novel α-agarase enzyme produced by the Thalassomonas sp. LD5 strain. However, the structural and functional characteristics of α-AgaD within biological systems remain largely unexplored. This study therefore aims to provide a comprehensive in silico analysis of α-AgaD, focusing on its physicochemical properties, phylogenetic relationships, secondary structure composition, and 3D homology modelling. A range of computational tools was employed to validate the findings and enhance the structural understanding of this newly identified α-AgaD enzyme. The α-AgaD protein consists of 1,466 amino acids with a molecular weight of 158,787.82 Da. It has a theoretical isoelectric point (pI) of 4.14, indicating an overall acidic nature. Structural analysis revealed that alpha helices and random coils are the predominant secondary structures. Hydrophobic amino acids were more abundant than hydrophilic ones, with glycine accounting for approximately 10.4% of the total residues. The protein's aliphatic index was 72.05, and the instability index was 28.28, suggesting that α-AgaD is stable and likely to maintain its structure across a wide temperature range. Three-dimensional models of α-AgaD were constructed using I-TASSER, NCBI-PDB, SWISS-MODEL, and AlphaFold2, and subsequently validated using ERRAT, Verify3D, and PROCHECK. Among the models generated, AlphaFold2 produced the most accurate prediction, with nearly all amino acid residues located in the preferred regions of the Ramachandran plot. This further confirmed the reliability and quality of the refined models. The in silico structural analysis of α-AgaD offers valuable insights into the enzyme’s primary sequence, functional domains, and overall structural architecture, enhancing our understanding of α-agarase from Thalassomonas sp. LD5.
Bioethanol Production from Oil Palm Empty Fruit Bunches Using R5I3 Isolate Immobilized on Sodium Alginate Beads Ida Bagus Wayan, Gunam; Yanti Sinurat, Dame; Wartini, Ni Made
Indonesian Green Technology Journal Vol. 14 No. 1 (2025): Indonesian Green Technology Journal
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.igtj.2025.014.01.04

Abstract

Indonesia is the world's largest palm oil producer, contributing 59% of the total global production. The decreasing availability of fossil fuels contrasts with the increasing energy demand due to population growth. The development of bioethanol as an alternative energy source is expected to replace fossil fuels while contributing to environmental sustainability. Oil palm empty fruit bunches (OPEFB) are abundant industrial waste which is a potential raw material for bioethanol production. This study aims to analyze two different R5I3 cell treatments and fermentation time on bioethanol production from OPEFB and to determine the type of cell and fermentation time that produces the highest bioethanol content. The experiment was conducted using a Randomized Block Design with two factors: cell treatment types (free and immobilized cells) and fermentation time (24, 48, 72, and 96 hours). The results showed that cell treatment types significantly affected reducing sugar content, pH, total dissolved solids, and ethanol concentration. Fermentation time influenced reducing sugar content, total dissolved solids, and ethanol concentration but had no significant effect on pH. The treatment of immobilized R5I3 cells with a fermentation time of 72 hours produced bioethanol with the highest ethanol content of 1.02% (v/v).
Analisis Tingkat Kepuasan Konsumen Terhadap Kualitas Produk Mi Nyemek Di Warkop Mitra 88 Menggunakan Metode Quality Function Deployment (QFD) Erlangga, Muhammad Agung; Satriawan, I Ketut; Gunam, Ida Bagus Wayan
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 13 No 2 (2025): Juni
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JRMA.2025.v13.i02.p01

Abstract

Warkop Mitra 88 is a business in the food and beverage sector that began operations in 2024 and is required to maintain its position amidst competitors selling similar products, namely Mi Nyemek. The aim of this study is to identify the Mi Nyemek attributes considered important by consumers, analyze the importance and satisfaction levels of the Mi Nyemek product, and develop effective strategies to improve the quality of Mi Nyemek at Warkop Mitra 88. The research employs consumer satisfaction analysis using the Quality Function Deployment (QFD) method. The sample used in this study consists of 92 respondents. The findings reveal nine consumer importance attributes, with the highest importance value being the aroma attribute (4.03). Additionally, the attribute with the highest satisfaction level is price (4.35). The quality improvement strategy for Mi Nyemek prioritizes attributes with the highest improvement ratio values, namely maturity level, broth thickness, and aroma. Improvements are recommended in the technical parameters of product preparation, particularly in cooking Mi Nyemek, adding supplementary ingredients, and boiling noodles.
Pengaruh Penambahan Konsentrasi Gula dan Lama Fermentasi pada Proses Pembuatan Sweet Dessert Wine Buah Naga Super Red Gede Bagus Suwarrizki G. P; Ida Bagus Wayan Gunam; I Made Mahaputra Wijaya
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol. 4 No. 1 (2019): April
Publisher : Fakultas Teknologi Pertanian, Universitas Udayana Gedung GA, Fakultas Teknologi Pertanian, Universitas Udayana Jl. Raya Bukit Jimbaran, Jimbaran, Kuta Selatan, Bali Telp/Fax: (0361) 701801

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA.2019.v04.i01.p06

Abstract

Tujuan dari penelitian ini adalah untuk untuk mengetahui bagaimana pengaruh penambahan konsentrasi gula pasir dan lama fermentasi dalam proses pembuatan sweet dessert wine buah naga super red terhadap karakteristik produk sweet dessert wine dari buah naga super red serta untuk mengetahui perlakuan penambahan konsentrasi gula pasir dan lama fermentasi manakah yang menghasilkan karakteristik sweet wine dari buah naga super red terbaik. Hasil dari analisis keragaman menunjukkan bahwa faktor penambahan konsentrasi gula berpengaruh sangat nyata terhadap kadar etanol, total padatan terlarut dan total gula, tetapi tidak berpengaruh nyata terhadap pH dan total fenol. Faktor lama fermentasi berpengaruh sangat nyata terhadap total etanol, total padatan terlarut, total gula, pH dan total fenol. Interaksi perlakuan penambahan konsentrasi gula dan lama fermentasi berpengaruh sangat nyata terhadap total alkohol, total padatan terlarut dan total gula. Dari hasil uji sensoris didapatkan bahwa perlakuan penambahan konsentrasi gula hingga 25°Brix dan lama fermentasi 15 hari memiliki karakteristik: warna merah tua agak jernih hingga merah tua jernih, aroma fruity hingga aroma sangat fruity, rasa agak suka hingga suka dan penerimaan secara keseluruhan suka hingga sangat suka, dengan karakteristik kimia sebagai berikut: kadar etanol 8,13% v/v, kadar metanol negatif, total padatan terlarut 9,9°Brix, total gula 2,49% b/v, pH 3,5 dan total fenol 0,882mG/100G.
Kajian Mengenai Perkembangan dan Pengembangan Kain Tenun Ikat Endek Klungkung dalam Perspektif Industri dan Budaya Cokorda Anom Bayu Sadyasmara; Ida Bagus Wayan Gunam
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol. 2 No. 1 (2017): April
Publisher : Fakultas Teknologi Pertanian, Universitas Udayana Gedung GA, Fakultas Teknologi Pertanian, Universitas Udayana Jl. Raya Bukit Jimbaran, Jimbaran, Kuta Selatan, Bali Telp/Fax: (0361) 701801

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA/2017.v02.i01.p08

Abstract

Tujuan dari penelitian ini adalah sebagai percontohan pola pergeseran aktivitas Masyarakat Bali melalui sebuah komoditas, yang dalam hal ini adalah komoditas berbasis budaya dan melihat pola perkembangan dan pengembangan Endek sebagai sebuah komoditas berbasis budaya di Kabupaten Klungkung. Studi ini menggunakan pendekatan gabungan dari metode penelitian historis, etnologi, deskriptif kualitatif dan kultural. Pengumpulan data dilakukan dengan teknik observasi dan wawancara langsung kepada para stakeholder yang ditentukan menggunakan metode purposive dan snowball sampling. Kerajinan tenun endek di Klungkung dapat menggambarkan adanya pergeseran aktivitas pada masyarakat di Klungkung. Aktivitas menenun yang semula merupakan aktivitas sekunder kemudian berkembang ataupun bergeser menjadi salah satu aktivitas primer (mata pencaharian). Bergesernya fungsi aktivitas adalah sebagai akibat dari bergesernya fungsi dari kain endek yang sebelumnya adalah artefak bergeser menjadi komoditas. Pola perkembangan dan pengembangan yang terjadi pada kain endek di gerakkan oleh proses adopsi teknologi, yakni teknologi yang terkandung pada fasilitas rekayasa (ATBM), teknologi yang terkandung dalam informasi yakni media (cetak, televisi, internet), teknologi yang terkandung pada manusia (Observasi, Experience) dan teknologi yang terkandung dalam organisasi (proses akulturasi). Proses adopsi teknologi mengakibatkan terjadinya perkembangan melalui sebuah pengembangan yang kemudian menghasilkan bentuk baru dikenal dengan istilah komodifikasi.
Pengaruh Konsentrasi Starter dan Gula terhadap Karakteristik Wine Salak Ida Bagus Wayan Gunam
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol. 3 No. 1 (2018): April
Publisher : Fakultas Teknologi Pertanian, Universitas Udayana Gedung GA, Fakultas Teknologi Pertanian, Universitas Udayana Jl. Raya Bukit Jimbaran, Jimbaran, Kuta Selatan, Bali Telp/Fax: (0361) 701801

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA.2018.v03.i01.p05

Abstract

Penelitian ini bertujuan untuk menentukan konsentrasi starter dan penambahan gula yang optimal untuk memperoleh karakteristik wine salak yang terbaik. Penelitian ini menggunakan Rancangan Acak Kelompok pola factorial dengan dua faktor. Faktor pertama adalah konsentrasi starter yaitu: 5, 10 dan 15% (v/v) dan faktor kedua adalah penambahan gula yaitu: 10, 15, 20 dan 25% (b/v). Hasil penelitian menunjukkan bahwa konsentrasi starter dan penambahan gula berpengaruh terhadap karakteristik wine salak. Pada konsentrasi gula yang lebih tinggi dapat menhasilkan kadar alkohol yang lebih tinggi juga, karena terdapat lebih banyak substrat yang dimanfaatkan oleh khamir untuk memproduksi alkohol. Wine salak dengan karakteristik terbaik diperoleh dari perlakuan konsentrasi starter 15% (v/v) dan penambahan gula 25% (b/v), dengan kadar alkohol tertinggi yaitu sebesar 12,25% dan juga memiliki nilai uji efektifitas terbaik yaitu sebesar 1,33.
Deteksi dan Indentifikasi Cemaran Kimiawi dan Mikrobiologis pada Sumber Air Tradisional (Beji) di Dataran Rendah Bali Selatan I M. Mahaputra Wijaya; I W. Wisma P Putra; I B. Wayan Gunam; I B. K. Widnyana Yoga; I W Suarta
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol. 7 No. 1 (2022): April
Publisher : Fakultas Teknologi Pertanian, Universitas Udayana Gedung GA, Fakultas Teknologi Pertanian, Universitas Udayana Jl. Raya Bukit Jimbaran, Jimbaran, Kuta Selatan, Bali Telp/Fax: (0361) 701801

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA.2022.v01.i01.p04

Abstract

This study was aimed to determine the safety of Balinese local traditional water sources (beji) which are commonly used by people in lowland villages in Bali for their daily needs. In this work both chemical and biological aspects were studied, namely the mineral content, heavy metals, and the content of harmful bacteria (such as E. coli and coliforms, others, Shigella, Salmonella, and others) which might exist in the spring waters using with modified guidelines from SNI on drinking water. The research was conducted by collecting water samples from springs (beji) located in the lowlands of southern Bali. Chemical contamination measurement on heavy metal content was carried out using Atomic Absorption Spectroscopy (AAS) and the dissolved particles was observed using UV-visible Spectroscopy. The spring water samples were then grown on specific media to identify the contaminating bacteria. Twenty nine samples of spring water from Klungkung, Gianyar, Denpasar, Badung, and Tabanan regencies were collected then the physical characteristics were measured. The chemical and biological contamination tests on the spring water samples were carried out and no samples were found to be chemically or biologically polluted, and the quality of the all spring waters was deemed acceptable in the SNI regarding drinking water.
Karakteristik Sawi Hijau (Brassica rapa var parachinensis) yang Dihasilkan dari Aplikasi Bakteri Pemacu Pertumbuhan I Gusti Ayu Lani Triani; Ida Bagus Wayan Gunam
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol. 7 No. 1 (2022): April
Publisher : Fakultas Teknologi Pertanian, Universitas Udayana Gedung GA, Fakultas Teknologi Pertanian, Universitas Udayana Jl. Raya Bukit Jimbaran, Jimbaran, Kuta Selatan, Bali Telp/Fax: (0361) 701801

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA.2022.v01.i01.p08

Abstract

This study tries to use the roots of bamboo plants that grow in Bali as Plant Growth Promoting Rhizobacteria and applied to vegetable plants to see the physical characteristics of these vegetables. This study aims to determine the physical characteristics of greens mustard produced by using the application of growth-promoting bacteria derived from the roots of bamboo (Bambusa maculata). The characteristics of the greens mustard were compared with the use of commercial PGPR (products from Central Java farmers) and without the use of growth-promoting bacteria. Based on the results of the research on the physical characteristics of greens mustard (Brassica rapa var parachinensis) the results of the application of growth-promoting bacteria from bamboo roots obtained plant heights ranging from 39 cm, weight 34 – 44 g, number of leaves 9 – 12 pieces, texture 22.18 – 30.31 kg .m/sec2, brightness level 42.13 – 47.13 and total dissolved solids 4.4 – 5.1% brix, slightly different from the treatment without PGPR and commercial PGPR. For this reason, it is necessary to continue cultivation with growth-promoting bacteria derived from natural ingredients that grow in Bali, this is an effort towards environmentally friendly cultivation so that it is expected to get quality results and are safe for consumption.
Co-Authors A.A.S. A. Sukmaningsih Ab Wahabb, Roswanira Abdul Wahab, Roswanira Adekilekun , Habeebulahi Ajibola Adekilekun Oyewusi, Habeebat Adekilekun, Habeebulahi Akinyede, Kolajo Ambar Kusumaningrum Amna Hartiati Anak Agung Made Dewi Anggreni Azis Akbar Hakim Bambang Admadi Harsojuwono Bimby Issassam Cokorda Anom Bayu Sadyasmara Damuri, Nur Wahida Deddy Setiadi Kadir Madjid Desi N. I. Izzabilah Dwi Ayu Kirani Paramita Dylla Hanggaeni Dyah Puspaningrum Ella Dewi Yusnia ENIEK KRISWIYANTI Erlangga, Muhammad Agung Fitra Ayu Sitanggang G.P. Ganda Putra Gde Agung Bagus Surya Adnyana Gede Bagus Suwarrizki G. P Hamonangan Sipayung Huda , Nurul Huyop, Fahrul I B. K. Widnyana Yoga I B. K. Widnyana Yoga I Dewa Gde Mayun Permana I Dewa Putu Rega Elyana I Gede Arya Sujana I Gede Arya Sujana I Gede Arya Sujana I Gede Krisna Putra Pratama I Gusti Ayu Lani Triani I Gusti Ayu Lani Triani I Gusti Bagus Ananta Wijaya Putra I Gusti Ngurah Agung Paranatha I Kadek Adi Wijaya Putra I Ketut Satriawan I Komang Trikuti I M. Mahaputra Wijaya I M. Mahaputra Wijaya I M. Mahaputra Wijaya I M. Mahaputra Wijaya I Made Griya Adi Parta I Made Mahaputra Wijaya I Made Mahaputra Wijaya I Made Mahaputra Wijaya I Made Mahaputra Wijaya I Made Sugitha I MADE SUPARTHA UTAMA I Made Supartha Utama I Made Yoga Saputra I MADE YOGA SEMARA GUNA I Putu Hendra Prasetya I W Suarta I W W Aryanika I W. Wisma P Putra I W. Wisma P. Putra I W.G. SEDANA YOGA I Wayan Arnata I Wayan Arnata I Wayan Arnata I Wayan Kasa I Wayan Suarta I Wayan Widia I Wayan Wisma Pradnyana Putra I Wayan Wisma Pradnyana Putra I.M.S. Wijaya IDA AYU ASTARINI Ida Ayu E. P. Wulandari Ida Ayu Pridari Tantri Ida Bagus Gede Brahmantara IDA BAGUS N. SURYA DARMA Ida Bagus Wisnu Matra Atmaja Indah S. R. Naibaho Indah Sri Rejeki Naibaho Izzah Ismail, Norjihada Jamas Prameshwari Janurianti , Ni Made Defy Janurianti, Ni Made Defy Ketut Agus Ary Subakti KETUT BUDA KOMANG AYU NOCIANITRI Kozo Asano Luh Dian Rna Fajarini Luh Putu Wrasiati Lutfi Suhendra Made Ria Defiani Mahaputra Wijaya Mertayasa, I Made Michael Jordi Theofanny Mohd Rozdhi, Amira Azawani Mohd Shariff , Amir Husni Monalisa Nababan Monikasari, Ni Nyoman Trisa Muhammad Iqbal N.P.I. Widyantari Nebay Cronika Simbolon Ni Kadek Eni Juniantari Ni Made Ayu Suardani Singapurwa Ni Made Ratih Despianti Ni Made Wartini Ni Putu Puspadi Aristyanti Ni Putu Suwariani Ni Wayan Wisaniyasa Noval Wahyu Adi Nursatria Purba Nurul Octavia Wasis NYOMAN SEMADI ANTARA Odilia Keron Oladimeji Tugbobo, Samuel Oladipo, Oluwatosin Oyewusi, Habeebat Adekilekun Pande Ketut Diah Kencana Pande Komang Suparyana Pavalee Chompoorat Tridtitanakiat Purnami, Ni Gusti Ayu Kade Sintya Ari Putu Agus Nadiarta Putu Ari Sandhi Wipradnyadewi - Putu Diah Wahyuni Putu Setia Budi Rajindran, Nanthini Rendy Sinaga Rike Pratiwi Sadyasmara, Cokorda Anom Bayu Sandhi Wipradnyadewi, Putu Ari Sayi Hatiningsih Shenni Maulina Suputra, I Gede Maha Adijaya Teguh Pribadi Girsang Teruo Sone Thobie E. B Kaban Tirmizhi Abubakar, Munkaila WAYAN REDI ARYANTA Wito Setioko Yanti Sinurat, Dame Yohanes Setiyo Yohanes Setiyo Yohannes Eko Putra Simanullang Yudisthira Dharma Bhusana Dasa Zainul Arifin