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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotekno METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Itepa : Jurnal Ilmu dan Teknologi Pangan Jurnal Veteriner Jurnal Biologi Udayana SIMBIOSIS Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Indonesian Journal of Biotechnology Jurnal Ilmiah Teknologi Pertanian Agrotechno Jurnal Teknologi & Industri Hasil Pertanian Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Teknologi Indonesia Canrea Journal: Food Technology, Nutritions, and Culinary Journal Jurnal Gastronomi Indonesia AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) SEAS (Sustainable Environment Agricultural Science) Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA)

Ida Bagus Wayan Gunam

Program Studi Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Universitas Udayana, Badung, Bali, Indonesia.

Author-ID : 2929439

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Social Sciences Veterinary

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ISOLATION MICROBE Indigenous TO DEGRADE PROFENOFOS FROM SOIL BEDUGUL AREA I Wayan Wisma Pradnyana Putra; Ida Bagus Wayan Gunam; A.A.M. Dewi Anggreni
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 3 No 2 (2015): Juni
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (533.483 KB)

This study aimed to isolate microbe indiegenous. Samples will takes of soil that has been contaminated by pesticides in Bedugul area. In addition, experiments conduct to determine resistance isolates at high concentrations profenofos. The result of the research showed 6 isolates. Isolates with IPP 02 code, was good growth on Mineral Salt Peptone Yeast medium with 200 ppm concentration profenofos. Keywords : isolation, screening, idiegenous

ISOLASI BAKTERI SELULOLITIK PENDEGRADASI SELULOSA DARI KOMPOS Zainul Arifin; Ida Bagus Wayan Gunam; Nyoman Semadi Antara; Yohanes Setiyo
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 7 No 1 (2019): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

This study aimed to isolate cellulolytic bacteria that have the potential to degrade cellulose taken compost samples from Temesi and Bondowoso, this study was also conducted to determine the ability of bacteria to degrade cellulose based on Congo red test and filter paper degradation test. All isolates were tested for cellulolytic activity using soluble Carboxymethyl Cellulose (CMC). Characterization was carried out by growing selected pure isolates on CMC media and then dripping 0.1% congo red to test the cellulolytic potential (cellulolytic potential was characterized by the emergence of clear zones around the colony). The results of isolation of bacteria obtained 38 isolates, namely 26 Bondowoso sample isolates and 12 Temesi isolates that were able to grow and utilize cellulose as a carbon source. But only fourteen isolates produced clear zones in the Congo red test with diameters ranging from 1.66 cm to 6.76 cm. Six isolates that have the largest diameters clear zone, were tested for degradation of filter paper (Whatman no. 1). Isolates bacteria of B2S8 obtained from Bondowoso compost samples has the highest ability to degrade cellulose on Whatman paper no. 1 as much as 51.30%. Keywords: Compost, Isolation, Screening, Cellulolytic Bacteria.

Pengaruh Perlakuan pH dan Suhu terhadap Stabilitas Ekstrak Alga Merah (Gracilaria sp.) sebagai Pewarna Alami Odilia Keron; Luh Putu Wrasiati; Ida Bagus Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 9 No 3 (2021): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Phycoerytrin is a red pigment found in red algae. Red algae Gracilaria sp. potential to be a source of natural dye because it contains phycoerythrin. Phycoerythrin is unstable to light, pH, and temperature. The purpose of this study was to determine the effect of pH and temperature on the stability of the dye extract from the red algae Gracilaria sp. and determine the pH and temperature that best maintain the constancy of the red algae Gracilaria sp. extract during storage. The design in this study used a completely randomized design with two factors, namely pH and temperature. First factor was the pH treatment which consist of three levels namely pH 4, pH 7 and pH 10 and second factor was initial temperature consist of two levels, namely 4±2?C and 28±2?C. The results showed that pH had a very significant effect (P<0.01) on the total phycoerythrin, brightness (L*), redness (a*) and yellowness (b*) at weeks 2, 3, 4. Temperature treatment has a very significant effect on the total phycoerythrin and brightness (L*) at weeks 3 and 4, as well the level redness (a*) and the level yellowness (b*) at weeks 2, 3 and 4. At pH 4 and cold temperature (4±2?C) were the best treatments to maintain the stability of the red algae Gracilaria sp. extract during storage. They had the lowest decrease in phycoerythrin levels and redness (a*), and had the smallest increase in brightness (L*) and yellowness (b*). Extract that were stored for 4 weeks with pH 4 and temperature 4±2?C treatment had decrease in total phycoerythrin, increase in brightness (L*), decrease in redness (a*), and increase in yellowness (b*) successively was 13.04%, 0.47%, 6.58%, dan 3.50%. Keywords : Gracilaria sp. extract, phycoeriythrin, pH, temperature, stability

Pengujian Bakteri Potensial Pendegradasi Dibenzothiophene (DBT) yang diisolasi dari Tanah yang Terkontaminasi Minyak Bumi di Samboja Bimby Issassam; Ida Bagus Wayan Gunam; Ni Made Wartini
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 4 No 3 (2016): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.962 KB)

This study aims to determine the dibenzothiophene (DBT) degradation level of four potential sulfur aromatic degrading bacteria. Biodesulfurization activity test was done by using twophase system. First was water phase which used mineral salt sulfur free (MSSF) medium and the second phase was oil phase which use 200 ppm DBT in n-tetradecane. Isolates were incubated for 96 hours at 37oC and the DBT residu oil phase was analyzed using Gas Chromatography. The experiment was repeated twice. This isolate wa able to degrade DBT in level of 80,82% after 96 hours incubation. The growth of the bacteria in water phase reached OD6601,169. Keywords: Dibenzothipohene, Biodesulfurization, Bacteria Potential, SBJ 8

Isolasi dan Karakterisasi Bakteri Penghasil Etanol dari Lingkungan Industri Arak Bali di Desa Merita dan Tri Eka Buana, Karangasem-Bali Azis Akbar Hakim; I M. Mahaputra Wijaya; Ida Bagus Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 8 No 2 (2020): Juni
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

The purpose of this research is isolation and characterization to find potential bacteria which can produce the most optimal ethanol from the Arak Bali industry in Karangasem Regency, Bali. Bacteria were isolated by exposure method in open air using selective media Zymomonas Sucrose Medium (ZSMA) with the addition of nystatin as much as 0.18 g / L as an antifungal then samples were taken at three different points in one Arak Bali production location, namely the distillation place, the fermentation room for roomie, and the place of taking coconut juice under the coconut tree and the variation of time is 15, 30, and 60 minutes of exposure. Gas checking is done on the bacteria obtained to select its ability to produce ethanol. The results of the scanning of 11 best isolates using UV-visible spectrophotometry were fermented on 500 mL ZSM media for 10 days. BM1-CP14 is the best isolate to produce total ethanol of 15.33 mL through the fermentation process. The results of the characterization of BM1-CP14 isolates were Gram-positive bacteria in the form of bacilli, anaerobic and non-motile bacteria. The results showed that bacteria isolated from open-air also can produce ethanol. Keyword: ethanol, Arak Bali, airborne bacterial exposure, isolation, characterization, UV-Visible spectrophotometry

Skrining Isolat Bakteri Asam Laktat Penghasil Bakteriosin yang Diisolasi dari Asinan Rebung Bambu Tabah dan Ketahanannya terhadap Panas I Gede Arya Sujana; Nyoman Semadi Antara; Ida Bagus Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 8 No 4 (2020): Desember
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

The aim of this study to determine the Lactic Acid Bacteria (LAB) isolated from pickled tabah bamboo shoots can produce bacteriocins and the resistance to heat. This research was conducted in two stages, namely: 1) Screening of LAB isolates producing bacteriocin and 2) Testing the effect of heating temperatures of 80°C, 100°C and 121°C for 5, 10 and 15 minutes and without heating to bacteriocin resistance activity. In this study, the screening stage was carried out in two stages, namely: 1) antibacterial test with cell free supernatant and 2) antibacterial test with neutralized cell free supernatant. The resulting clear zone is observed then followed by calculating the value of Arbitrary Units (AU). The bacteria used as an indicator is Lactobacillus plantarum which is Gram positive bacteria. The results of this study indicated that of 88 LAB isolates there were 27 isolates that were able to produce bacteriocin. From 27 isolates, there were three isolates with the most potential to produce bacteriocins, namely isolate PR.6.10.5 with an inhibition zone of 17.67 mm and a bacteriocin activity value of 264.81 mm2/ml, isolate PR.6.15.2 with an inhibition zone of 17.33 mm and the bacteriocin activity value of 259.57 mm2/ml and isolate PR.3.15.1 with an inhibition zone of 17.00 mm and a bacteriocin activity value of 254.34 mm2/ml. Bacteriocins from LAB isolates isolated from pickled tabah bamboo shoots were able to withstand heating to a temperature of 121°C for 15 minutes. Bacteriocin activity decreased by an average of 27.75% after heating at 80°C, 100°C and 121°C for 5,10 and 15 minutes with a bacteriocin activity value from 141.82 mm2/ml to 254.34 mm2/ml. The smallest decrease in activity value occurred in PR.6.10.5 isolates with an average decrease of 22.98%. Keywords: Screening, lactic acid bacteria (LAB), bacteriocin, heat.

INHIBITION ACTIVITY of ESSENTIAL OIL of LEMONGRASS LEAVES (Cymbopogon citratus) ON THE GROWTH OF Escherichia coli, Staphylococcus aureus, AND Vibrio cholerae. Dwi Ayu Kirani Paramita; Nyoman Semadi Antara; Ida Bagus Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 2 No 1 (2014): Maret
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.412 KB)

The research aim was to determine the inhibition activity of essential oil of lemongrass (Cymbopogon citratus) on the growth of pathogenic bacteria. This research used a Completely Randomized Design with 5 treatments lemongrass oil concentration in 1% tween 80 namely 1%, 2%, 3%, 4%, 5% (v/v), and each concentration was tested to the three bacteria (Escherichia coli, Staphylococcus aureus, and Vibrio cholerae). Each treatment was repeated 3 times and the data were analyzed by analysis of variance, and followed by Duncan's Multiple Range Test. The results showed that lemongrass oil in tween 80 affected significantly the growth of Escherichia coli, Staphylococcus aureus and Vibrio cholerae. Inhibition against Escherichia coli, Staphylococcus aureus, and Vibrio cholerae at a concentration of 1% produces the highest activity with the inhibition diameter of 6,03 mm, 4,97 mm, and 9,67 mm, respectively. Vibrio cholerae was more sensitive againts lemongrass oil than Escherichia coli and Staphylococcus aureus.

KARAKTERISTIK CUKA DARI CAIRAN PULPA HASIL SAMPING FERMENTASI BIJI KAKAO (Theobroma cacao L.) PADA PERLAKUAN PENAMBAHAN INOKULUM Acetobacter aceti DAN LAMA FERMENTASI Yudisthira Dharma Bhusana Dasa; G.P. Ganda Putra; I.B. Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 5 No 2 (2017): April
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.627 KB)

This aims to this study were to (1) determine the effect of addition inoculum Acetobacter aceti andfermentation period on the characteristics of vinegar fermentation from liquid waste of cocoa beansfermentation, and (2) to determine the accurate of addition innoculum Acetobacter aceti and fermentationperiod which one produces the best characteristic fermentation from liquid waste of cacao beansfermentation. The experiments was designed by a Split Plot design with the addition of inoculumAcetobacter aceti into the main plot consisting of 3 plots 10%. 15%. and 20% (v/v). Fermentation period isa subplots consisting of 6 levels were 0. 5. 10. 15. 20. 25 days. The fermentation period was affectedsignificantly (P <0,01) on levels of acetic acid, alcohol content and total dissolved solids from a liquid pulpvinegar fermentation from liquid waste of cacao beans fermentation, while the addition of inoculumAcetobacter aceti and interaction did not affect (P> 0,05). The best characteristics of fermentation vinegarwas found on the addition of an inoculum of 20% and fermentation period for 25 days with the acetic acidcontent, alcohol content and total of soluble solid were 3.21 (% v/v), 0.00%, and 4.79 oBrix. respectively.

PENGARUH PERBEDAAN SUHU DAN SUMBER KARBON PADA PERTUMBUHAN Pseudomonas sp. STRAIN LSU20 DALAM MENDESULFURISASI DIBENZOTIOFENA Muhammad Iqbal; Ida Bagus Wayan Gunam; I Wayan Arnata
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 4 No 3 (2016): September
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.476 KB)

Desulfuriztion of crude oil to be low sulfur fuel content is a main concern by all researcher. Pseudomonas sp. strain LSU20 known as one of bacteria which could degrade dibenzothiphene(DBT) on n-tetradecane (TD) oil model. Temperature Growth condition of this bacteria wasexperimented at 37ºC and 45ºC. Some carbon sources were also tried in this experiment, such asglucose, sucrose, and citric acid. Using glucose as source of carbon and temperature growth condition at 37ºC, the bacteria could degrade 67% (DBT) in TD after 96 hours of incubation. In this condition the bacteria growth could reach OD660nmup to 0,978. Key: LSU20, dibenzothiophene, n-tetradecane, biodesulfurization.

PENGARUH KONSENTRASI Na-ALGINAT DAN UKURAN BEADS TERHADAP STABILITAS BEADS DAN AKTIVITAS SEL Agrobacterium tumefaciens LSU20 IMMOBIL DALAM BIODESULFURISASI DIBENZOTHIOFENA I Made Yoga Saputra; Nyoman Semadi Antara; Ida Bagus Wayan Gunam
JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI Vol 6 No 2 (2018): April
Publisher : Department of Agroindustrial Technology, Faculty of Agricultural Technology, Udayana University

The purpose of this study was to determine the concentration and size of Na-alginate beads that have the highest degradation activity of dibenzothiophene. Biodesulfurization (BDS) of dibenzothiophene (DBT) was performed using 3 Na-alginate concentrations and 3 different beads sizes in the oil model system. Biodesulfurization was performed with incubation for 24 hours. The previous research showed that sodium alginate (Na-alginate) was an appropriate immobilizing agent compared to other immobilized materials. Na-alginate 4% show the activity of the degradation of the most good that is 66.33% (bead size 2 mm), 62.99% (bead size 3 mm), 59.93% (bead size 4 mm), for concentration of 3% Na-alginate showed 65.58% (bead size 2 mm), 61.68% (bead size 3 mm) and 60.43% (bead size 4 mm), while concentration 5% showed the most low that is 64.86% (bead size 2 mm), 61.01% (bead size 3 mm), and 58.89% (bead size 4 mm). The stability test showed Na-alginate 4% have the stability and durability of the bead stronger, the test showed Na-alginate can be used up to five repeat and still have degradation activity. Key words: Biodesulfurization, Dibenzothiophene, Immobilized cells, Na-alginate.

Co-Authors A.A.S. A. Sukmaningsih Ab Wahabb, Roswanira Abdul Wahab, Roswanira Adekilekun , Habeebulahi Ajibola Adekilekun Oyewusi, Habeebat Ambar Kusumaningrum Amna Hartiati Anak Agung Made Dewi Anggreni Azis Akbar Hakim Bambang Admadi Harsojuwono Bimby Issassam Damuri, Nur Wahida Deddy Setiadi Kadir Madjid Dwi Ayu Kirani Paramita Dylla Hanggaeni Dyah Puspaningrum Ella Dewi Yusnia ENIEK KRISWIYANTI Fitra Ayu Sitanggang G.P. Ganda Putra Gde Agung Bagus Surya Adnyana Gede Bagus Suwarrizki G. P Hamonangan Sipayung Huda , Nurul Huyop, Fahrul I B. K. Widnyana Yoga I Dewa Gde Mayun Permana I Dewa Putu Rega Elyana I Gede Arya Sujana I Gede Krisna Putra Pratama I Gusti Ayu Lani Triani I Gusti Bagus Ananta Wijaya Putra I Gusti Ngurah Agung Paranatha I Kadek Adi Wijaya Putra I Komang Trikuti I M. Mahaputra Wijaya I Made Mahaputra Wijaya I Made Mahaputra Wijaya I Made Sugitha I Made Supartha Utama I MADE SUPARTHA UTAMA I Made Yoga Saputra I MADE YOGA SEMARA GUNA I Putu Hendra Prasetya I W W Aryanika I W. Wisma P. Putra I W.G. SEDANA YOGA I Wayan Arnata I Wayan Kasa I Wayan Suarta I Wayan Widia I Wayan Wisma Pradnyana Putra I.M.S. Wijaya IDA AYU ASTARINI Ida Bagus Gede Brahmantara IDA BAGUS N. SURYA DARMA Ida Bagus Wisnu Matra Atmaja Izzah Ismail, Norjihada Janurianti , Ni Made Defy Janurianti, Ni Made Defy Ketut Agus Ary Subakti KETUT BUDA KOMANG AYU NOCIANITRI Kozo Asano Luh Putu Wrasiati Lutfi Suhendra Made Ria Defiani Mahaputra Wijaya Michael Jordi Theofanny Mohd Rozdhi, Amira Azawani Mohd Shariff , Amir Husni Monalisa Nababan Monikasari, Ni Nyoman Trisa Muhammad Iqbal N.P.I. Widyantari Nebay Cronika Simbolon Ni Kadek Eni Juniantari Ni Made Defy Janurianti Ni Made Ratih Despianti Ni Made Wartini Ni Putu Puspadi Aristyanti Ni Putu Suwariani Ni Wayan Wisaniyasa Noval Wahyu Adi Nursatria Purba Nurul Octavia Wasis NYOMAN SEMADI ANTARA Odilia Keron Oladipo, Oluwatosin Oyewusi, Habeebat Adekilekun Pande Ketut Diah Kencana Pande Komang Suparyana Putu Agus Nadiarta Putu Setia Budi Rajindran, Nanthini Rendy Sinaga Rike Pratiwi Sadyasmara, Cokorda Anom Bayu Sandhi Wipradnyadewi, Putu Ari Sayi Hatiningsih Shenni Maulina Teguh Pribadi Girsang Teruo Sone Thobie E. B Kaban Tirmizhi Abubakar, Munkaila WAYAN REDI ARYANTA Wito Setioko Yohanes Setiyo Yohannes Eko Putra Simanullang Yudisthira Dharma Bhusana Dasa Zainul Arifin

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