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All Journal International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Itepa : Jurnal Ilmu dan Teknologi Pangan Agrointek Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Wikrama Parahita : Jurnal Pengabdian Masyarakat Jurnal Aplikasi Teknologi Pangan Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Jurnal Ilmiah Teknologi Pertanian Agrotechno Itepa : Jurnal Ilmu dan Teknologi Pangan
I Putu Suparthana
Program Studi Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Industrial & Manufacturing Engineering Mechanical Engineering Public Health Social Sciences

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Food Safety and Nutrients of Terites, A Case Study in Kabanjahe, the city of Karo region, Sumatera Utara IPutu Suparthana; Ricky Suranta Perangin-angin; IGusti Ngurah Agung
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 2 No 2 (2015)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Terites is a traditional food of Karo’s people in Sumatera Utara which uniquely utilizematerials from cow’s rumen as a soup. This food is served, especially in the celebrating days,however now days terites is also served at the local restaurants in Kabanjahe, the capital city ofKaro region, Sumatera Utara. This study was aimed to investigate the food safety and nutrientcontents of terites which served in local restaurants in Kabanjahe. The investigations was usingpurposive sampling method, the samples were obtained from all of the restaurant which servedterites in Kabanjahe and analyze in the laboratory. The results showed that total microbe in thesamples was 2.4 x 104 cfu/g , negatively contaminated by E.coli and Salmonella spp. The dataare adequate to the national standard (SNI) for the food safety. The terites contains (average)19.9% protein, 15.4% fat and 1.68% crude fiber, which adequate as a good nutrient content.
Pengaruh Konsentrasi Gliserol Terhadap Karakteristik Edible Film Pati Ubi Jalar (Ipomoea batatas L.) Anjani Fatnasari; Komang Ayu Nocianitri; I Putu Suparthana
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 5 No 1 (2018): Scientific Journal of Food Technology
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Edible films is a thin layer packaged which produced from biopolymers and food-grade additives which is biodegradable in consumtion and environment. Sweet potato starch is one of many hydrocolloid which can be made as the material of edible film. Glycerol added in the formulation of edible film as a plasticizer to increase the characteristic of edible film. The purpose of this research is to determine glycerol concentration which it had best physic and chemist characteristics of edible film. The method of this research was completely randomized design with four grade of glycerol concentration there are 10%, 15%, 20% and 25% (v/b starch) which repeated four times. There are many parameters which analyzed in this research there are moisture content, thickness, water vapor transmission rate (WVTR), elongation and tensile strength. Data was analyzed with ANOVA and continued with Duncan’s (?=5%). The result of the research are increasing the glycerol concentration were influence amount of water moisture, thickness, elongation and tensile strength, but it is not influence the amount of water vapour transmission rate edible film. Best characteristic of edible film shown in formulation 10% (v/b starch) glycerol with amount of water moisture 12,50%, thickness 0,06 mm, WVTR 1,79 g/m2, elongation 8,75% and tensile strength 0,75 MPa.
Identifikasi Transgene pada Tanaman Padi (oryza sativa var. koshihikari) yang Ditransformasi dengan Bantuan Agrobacterium tumefaciens, Menggunakan Metode Tanpa Teknik Kultur Jaringan. I Putu Suparthana; Masahiro Nogawa; Mineo Kojima
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 1, No. 1, 2014
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Metode transformasi tanaman dengan bantuan Agrobacterium tumefaciens biasa dilakukan dengan melibatkan teknik kultur jaringan, akan tetapi teknik kultur jaringan memiliki beberapa kelemahan yaitu memerlukan suatu kondisi steril, memakan banyak waktu, sering terjadi mutasi dalam proses kultur in vitro dan sejumlah tanaman bersifat rekalsitran pada tahap regenerasi. Disisi lain metode baru (in planta transformation system) yang dikembangkan dalam penelitian ini mampu mengatasi kelemahan-kelemahan tersebut. Penelitian dilakukan pada tanaman padi dengan bantuan A. tumefaciens sebagai inokulum namun tidak melibatkan teknik kultur jaringan pada masa regenerasinya. Biji padi (Oryza sativa L. var. Koshihikari) direndam dalam air selama 2 hari, dengan demikian embrionya yang mengandung sel apikal meristem dapat diinokulasi dengan cara menusukkan jarum yang telah dicelupkan dalam inokulum. Biji padi yang telah diinokulasi selanjutnya ditumbuhkan dilapangan selayaknya pembenihan biasa tanpa perlakuan steril. Untuk menentukan keberhasilan teknik ini, dua jenis strain mutan A. tumefaciens (M-21 dan LBA4404) digunakan dalam transformasi. Mutan M-21 mengandung Tn5 tersisip dalam gen iaaM dan mutan LBA4404 membawa binari plasmid vektor. Transgen dari mutan M-21 dapat diidentifikasi dari perubahan fenotipe pada tanaman padi transgenik sedangkan dari mutan LBA4404 dapat diidentifikasi dengan uji histokimia dan ketahanan terhadap antibitik (hygromycin B).Kata kunci: in planta transformation, A. tumefaciens, transformation method
Studi Potensi Lactobacillus rhamnosus A6 Hasil Isolasi dari Air Susu Ibu Sebagai Starter Dalam Pembuatan Yoghurt Liya Paramita; I Putu Suparthana; N.M. Indri Hapsari Arihantana
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 4 No 2 (2017): Scientific Journal of Food Technology
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

The aim of this research was to determine the potential of Lactobacillus rhamnosus A6 isolated from breast milk as a starter in the manufacture of yoghurt. The random design uses for the research with one factor treatment using different types of starter such as L. rhamnosus A6 as single starter, Streptococcus thermophilus and L. bulgaricus, L. rhamnosus A6 and L. bulgaricus, L. rhamnosus A6 and S. thermophilus, and L. rhamnosus A6 with S. thermophilus and L. bulgaricus. Milk was pasteurized at 80°C for 15 minutes and then added 6% starter culture according to the treatment. Each treatment was repeated 3 times to obtain 15 experimental units. The data obtained were analyzed by ANOVA and if there was any difference followed by Duncan test (DMRT). The best treatment was L. rhamnosus A6 with S. thermophilus and L. bulgaricus with good characteristic which content 3,16% protein; 3,74 x 106 CFU/ml total LAB; pH of 4,64; 0,53% total lactic acid; 408,3 mPas viscosity; color (white and like), aroma (acid and like), flavor (acid and like), texture (thick and like), and overall acceptance (like).
Penelusuran terhadap Pangan Tradisional Hasil Fermentasi Kedelai (Sere Kedele) di Kabupaten Gianyar, Bali.dan Identifikasi Molekuler Terhadap Bakteria yang Terlibat dalam Proses Fermentasi Spontan I Putu Suparthana; Agus Selamat Duniaji; Masayuki Hashimoto
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 5 No 2 (2018): Scientific Journal of Food Technology (September)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Sere kedele is a Balinese traditional soybean fermented food which involving spontaneous fermentation process in its production. It's report-ed produced by the people in Southeast area of Bali Island. Despite the importance of this product for communities health, its lack the basic scientific data. This research was aimed to figure out the existence of this product nowadays and characterize the traditional production as-pect, a survey was conducted to collect the data in the field and analyze descriptively. Furthermore, to figure out the microbial aspect, isola-tion was carried out in the laboratory and identify the bacteria by using molecular techniques. The result showed that sere kedele is still known by the most people in Gianyar region, its vended in the traditional market which supplied by four producers which locate in three districts (of seven). The way to produce sere kedele was varying in boiling time, fermentation time and the step of adding spicy. All produc-er applied spontaneous fermentation in the bamboo container, at room temperature. Nine bacterial species identified from this sere kedele, four of them belong to Bacillus species (Bacillus cereus B. flexus, B. subtilis, B. thuringiensis) and the others are Weissella confusa, W. cibaria, Acinetobacter baumannii, Proteus mirabilis and Klebsiella pneumoniae. The bacterial species of sere kedele contain non-pathogenic (B. flexus, B. subtilis, W. confusa) and pathogenic bacteria. From the result of this study, sere kedele production by using starter culture is highly recommended.
PENGARUH PENAMBAHAN RAGI ROTI DAN WAKTU FERMENTASI TERHADAP KARAKTERISTIK CUKA DARI NIRA LONTAR (Borassus flabellifer Linn) Antonius Solo; G.P. Ganda Putra; I Putu Suparthana
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 6 No 1 (2019): Scientific Journal of Food Technology (March)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Indonesia's natural resources are rich in biodiversity which is a very prospective potential to bedeveloped. One of them is palm tree. The main result of palm tree was sap. Palm sap can be processedinto sugar or vinegar. This study aims to know the effect of addition of bread yeast and fermentationtime on the characteristics of vinegar, determine the best treatment of adding bread yeast and fermentation time to produce vinegar from sap palm (Borassus flabellifer Linn), and to know the stabilityof vinegar products for storage temperature. The experimental design used was factorial CompletelyRandomized Design with 2 factors, namely the addition of bread yeast (0%, 1%, 2%, 3%, 4% and 5%)and fermentation time (0, 10, 15, 20, 25 and 30 days). The data obtained were analyzed by ANOVA andif the treatment had a significant effect it was followed by the Duncan test. The results showed that (1)the treatment of adding bread yeast had an effect on acetate levels, alcohol content, total soluble solidsand pH; but does not affect total sugar; fermentation time affects the level of acetic acid, total sugar,alcohol content, total soluble solids and pH; while the interaction between treatments has an effect ontotal soluble solids and pH, but does not affect acetic acid levels, total sugar and alcohol content onvinegar from fermented of palm sap, (2) treatment of addition of 5% bread yeast with 30 daysfermentation time was the best treatment for producing vinegar, with characteristics of acetic acid levelof 2.72% v / v, total sugar 0.03%, alcohol content 0.68% and total soluble solids 2.60 °Brix; (3) thestability of vinegar products produced at various storage temperatures an affects the levels of aceticacid and total soluble solids.
Aplikasi Pati Talas Kimpul Termodifikasi Secara HMT (Heat Moisture Treatment) Pada Pembuatan Bakso Ayam I Putu Suparthana; I Nengah Kencana Putra; Ni Wayan Wisaniyasa
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 3 No 2 (2016)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Pengenyal merupakan bahan yang sangat penting dalam pembuatan bakso ayam karena dapat menghasilkan bakso dengan karakteristik kenyal, kompak dan juicy. Hingga saat ini, bahan pengenyal yang banyak dan umum digunakan adalah sodium tri poli fosfat (STPP). STPP merupakan bahan tambahan pangan sintetik, yang bila digunakan secara berlebihan akan beresiko pada kesehatan. Disis lain pati talas kimpul termodifikasi (PTKT) yang merupakan pati tipe C memiliki daya ikat air tinggi, stabilitas terhadap panas yang baik, dan kemampuan membentuk gel yang tinggi. Sifat-sifat ini dapat digunakan sebagai pengenyal alami menggantikan STTP. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan PTKT terhadap karakteristik bakso ayam, pengaruh jumlah penambahan PTKT yang optimal pada proses pembuatan bakso ayam, dan mengetahui karakteristik bakso ayam yang ditambahkan PTKT. Disain penelitian menggunakan rancangan acak kelompok dengan perlakuan berupa penambahan PTKT yang dilakukan dalam enam taraf (0,5,10,15,20 dan 25%) dan diulang sebanyak tiga kali. Data dianalisis dengan sidik ragam dan perbedaan antar nilai rata-rata diuji dengan Duncan’s multiple range test. Data kadar air tertinggi didapat pada taraf perlakuan 20%. Hasil analisis sensori menunjukkan adanya pengaruh pada karakteristik (aroma dan citarasa) bakso ayam. Jumlah penambahan PTKT yang paling disukai sebanyak 10% dan masih dapat diterima hingga 15%.
Sifat Fisik, Kimia, dan Sensori Mi Instant yang Terbuat dari Tepung Komposit Terigu dan Pati Kimpul Modifikasi I Nengah Kencana Putra; I Putu Suparthana; Anak Agung Istri Sri Wiadnyani
Jurnal Aplikasi Teknologi Pangan Vol 8, No 4 (2019): November 2019
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.856 KB) | DOI: 10.17728/jatp.5161

Abstract

Pati kimpul modifikasi (PKM) merupakan pati yang dibuat dari umbi kimpul yang diberikan perlakuan fisik atau kimia sehingga mempunyai sifat fungsional lebih baik dibandingkan dengan pati kimpul alami. Pada penelitian ini, dievaluasi pengaruh komposisi tepung komposit (terigu-PKM) terhadap sifat fisik, kimia, dan sensori mi instant. PKM dibuat menggunakan metode high moisture treatment (HMT), yaitu pemanasan pati pada suhu 110˚C pada kadar air 30% selama 10 jam. Tepung komposit dibuat dengan menggunakan berbagai perbandingan terigu dan PKM (90:10, 80:20, 70:30, 60:40, 50, dan 50). Selanjutnya, tepung komposit ini diolah menjadi mi instan, dan kemudian sifat fisik, kimia, dan sensori mi instan yang dihasilkan dievaluasi. Hasil penelitian menunjukkan perbandingan terigu dan PKM pada tepung komposit berpengaruh nyata terhadap kadar air, kadar protein, kadar karbohidrat, waktu pemasakan, kehilangan padatan akibat pemasakan (KPAP), dan daya serap air mi instan. Berdasarkan hasil uji sensori, tepung komposit terigu-PKM (80:20) menghasilkan mi instan terbaik. Komposisi zat gizi mi instan yang dihasilkan adalah: kadar air (2,74%), lemak (29,19%), protein (7,68%), dan karbohidrat (58,91%). Mi instan terigu-PKM (80:20) memiliki aroma lebih baik, waktu pemasakan lebih singkat, dan daya serap air lebih baik dibandingkan mi terigu 100%, namum memiliki KPAP lebih tinggi. Kesimpulannya, PKM dapat digunakan sebagai pensubstitusi sebagian terigu dalam pembuatan mi instant.Physical, Chemical, and Sensory Properties of Instant Noodles Prepared from Wheat - Modified Tannia Starch CompositeAbstractModified tannia starch (MTS) is the starch made from tannia tuber, which is provided a physical or chemical treatment so that it has better functional properties compared to native tannia starch. In this study, the effect of the composition of the wheat flour-MTS composite on the physical, chemical, and sensory properties of instant noodles was evaluated. MTS was produced by using the high moisture treatment (HMT) method, which was heating the starch at the temperature of 110˚C and moisture content of 30% for 10 hours. The composite flour was made with various ratios of wheat flour and MTS (90:10, 80:20, 70:30, 60:40, 50, and 50). Subsequently, the composite flour was processed into instant noodles, and then the physical, chemical, and sensory properties of the noodles produced were evaluated. The results showed the ratio of wheat flour and MTS had a significant effect on the water content, protein content, carbohydrate content, cooking time, cooking loss, and water absorption capacity of the instant noodle. Based on the results of sensory evaluation, the composite of wheat flour-MTS (80:20) could produce the best instant noodles. The nutrition composition of the instant noodles produced, namely: water content (2.74%), fat content (29.19%), protein content (7.68%), and carbohydrate content (58.91%). If compared to the 100% wheat flour instant noodles, the wheat flour-MTS (80:20) instant noodles has a better aroma, better water absorption capacity, and shorter cooking time, but it has a higher cooking loss. In conclusion, MTS can be used as a partial substitute for flour in making instant noodles.
Pengaruh Konsentrasi Sukrosa terhadap Karakteristik Minuman Probiotik Sari Buah Jambu Biji Merah dengan Isolat Lactobacillus sp. F213 Ni Komang Tri Handayani; Komang Ayu Nocianitri; Putu Suparthana
Jurnal Ilmu dan Teknologi Pangan (ITEPA) Vol 11 No 1 (2022): Jurnal ITEPA
Publisher : Department of Food Science and Technology Study Program, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/itepa.2022.v11.i01.p15

Abstract

This research was aimed to determine the effect of sucrose concentration on the characteristics of red guava probiotic juice with Lactobacillus sp. F213 isolates and to determine the right concentration of sucrose that produced the best characteristics of red guava probiotic juice. This research used a completely randomized design with concentration of sucrose as treatments, which consisted of six levels: 0%, 2%, 4%, 6%, 8% and 10%. Each treatment was repeated 3 times to obtain 18 units of samples. The data of lactic acid bacteria total were analyzed descriptively and the data of total sugar, total acid, pH, sensory properties were analyzed by Analysis of Variance, and if the treatment affected the observed parameters significantly (P<0,05), it was continued with Duncan Multiple Range Test. The results showed that concentration of sucrose significantly affected to total sugar, total acid, pH, taste (hedonic), sour taste (score), sweet taste (score) and overall acceptance (hedonic), but no significant effect on color (hedonic) and aroma (hedonic). The 8% sucrose concentration produced the best red guava probiotic juice with the characteristics of total LAB 8,56 Log CFU/ ml, total sugar 10,60%, total acid 0,12%, pH 4,06, the color and the aroma were preferred, with slightly sour and sweet taste were preferred, and overall acceptance was preferred.
Pengaruh Penambahan Puree Daun Kelor (Moringa oleifera Lamk.) Terhadap Karakteristik Nugget Ikan Kembung (Rastrelliger kanagurta) Komang Ayu Puspa Hapsari; I Made Sugitha; I Putu Suparthana
Jurnal Ilmu dan Teknologi Pangan (ITEPA) Vol 11 No 1 (2022): Jurnal ITEPA
Publisher : Department of Food Science and Technology Study Program, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/itepa.2022.v11.i01.p13

Abstract

This study aims to determine the effect of addition Moringa leaf puree on the characteristics of mackerel nuggets, and to find out the right addition of Moringa leaf puree to produce mackerel nuggets with the best characteristics. This research was based on completely randomized design (CRD) with by addition treatment of Moringa leaf puree consisting of 6 levels: 0%, 10%, 20%, 30%, 40% and 50%. Each treatment was repeated 3 times to obtain 18 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect followed by Duncan Multiple Range Test (DMRT). The results showed that the effect of adding Moringa leaf puree had a significant effect on water content, fat content, protein content, crude fiber content, antioxidant activity, taste, aroma, texture (hedonic and scoring), and overall acceptance. The addition of 20% Moringa leaf puree produced with the best characteristics with water content criteria of 44,18%, ash content of 1.47%, fat content of 11.49%, protein content of 8.85%, crude fiber content of 9.26%, antioxidant activity of 67.32%, IC50 3757.486 ppm, ordinary aroma, taste like, texture and overall acceptance liked.
Co-Authors AA. Devi Pradnyanita AAGN Anom Jambe Adina Juniati Vortina Panggabean Adina Juniati Vortina Panggabean Agus Selamet Duniaji Amira Putri Anak Agung Istri Sri Wiadnyani Andreani, Ni Putu Elvian Anjani Fatnasari Antonius Solo Asri Andriani Betari Nurrachmah Kusuma Putri Desak Nyoman Anggreni Bhaskara Dewi Citra Laksmi Agung Putri Elia Agatha Christiani Elicia Debora Marbun, Monica Elsa Fanora Sinaga Enrica Balanzi, Dhenabe Flora Anggreni Sitinjak G.P. Ganda Putra Gusti Ayu Kadek Diah Puspawati Hana Kusuma Brahmasari Hossiana Hariputeri I D. G. Mayun Permana I D.P. Kartika Pratiwi I Dewa Gde Mayun Permana I Ketut Suter I Made Adhi Dharma Parayana I Made Sugitha I Nengah Kencana Putra I Nyoman Larry Julianto I Nyoman Widhi Adnyana I Wayan Mudra, I Wayan Icha Nabillah IDG. Mayun Permana IGusti Ngurah Agung Iis Sholin Ketut Ayurina Singarsari Diasaputri KOMANG AYU NOCIANITRI Komang Ayu Puspa Hapsari Komang Triani Utami L. Paramita Liya Paramita Lucia Amitasya Sinaga Luh Komang Puspa Handayani Luh Putu Trisna Darmayanti Made Dwi Widyantari Masahiro Nogawa Masahiro Nogawa Masayuki Hashimoto Mia Primsa Ginting Mineo Kojima Mineo Kojima N.M. Yudantin Nathania Pusparani Nur Ni Komang Tri Handayani Ni Luh Cintya Febriani Ni Made Indri Hapsari Arihantana Ni Made Inten Kusuma Dewi Ni Made Susi Kartika Ni Made Wartini Ni Made Yudantini Ni Made Yusa Ni Made Yusa Ni Nyoman Budiasih Ni Nyoman Puspawati Ni Wayan Wisaniyasa Nyoman Geatrin Arumsari Pandu Prayugo Paramita Wulandari, Kadek Ratih Permadi, Giovanni Brewijaya Putra Pinky Natalia Samanta Putri Irdayanti Putri Nathasya br Lahi Putu Timur Ina Putu Yumiko Murdiasa Ricky Suranta Perangin-angin Saraswati Permatasari, Ni Gusti Ayu Sayi Hatiningsih Sayi Hatiningsih Sutriswati Rahayu, Endang Tsutomu Shimizu