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Penelusuran terhadap Pangan Tradisional Hasil Fermentasi Kedelai (Sere Kedele) di Kabupaten Gianyar, Bali.dan Identifikasi Molekuler Terhadap Bakteria yang Terlibat dalam Proses Fermentasi Spontan I Putu Suparthana; Agus Selamat Duniaji; Masayuki Hashimoto
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 5 No 2 (2018): Scientific Journal of Food Technology (September)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Sere kedele is a Balinese traditional soybean fermented food which involving spontaneous fermentation process in its production. It's report-ed produced by the people in Southeast area of Bali Island. Despite the importance of this product for communities health, its lack the basic scientific data. This research was aimed to figure out the existence of this product nowadays and characterize the traditional production as-pect, a survey was conducted to collect the data in the field and analyze descriptively. Furthermore, to figure out the microbial aspect, isola-tion was carried out in the laboratory and identify the bacteria by using molecular techniques. The result showed that sere kedele is still known by the most people in Gianyar region, its vended in the traditional market which supplied by four producers which locate in three districts (of seven). The way to produce sere kedele was varying in boiling time, fermentation time and the step of adding spicy. All produc-er applied spontaneous fermentation in the bamboo container, at room temperature. Nine bacterial species identified from this sere kedele, four of them belong to Bacillus species (Bacillus cereus B. flexus, B. subtilis, B. thuringiensis) and the others are Weissella confusa, W. cibaria, Acinetobacter baumannii, Proteus mirabilis and Klebsiella pneumoniae. The bacterial species of sere kedele contain non-pathogenic (B. flexus, B. subtilis, W. confusa) and pathogenic bacteria. From the result of this study, sere kedele production by using starter culture is highly recommended.
PENGARUH PENAMBAHAN RAGI ROTI DAN WAKTU FERMENTASI TERHADAP KARAKTERISTIK CUKA DARI NIRA LONTAR (Borassus flabellifer Linn) Antonius Solo; G.P. Ganda Putra; I Putu Suparthana
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 6 No 1 (2019): Scientific Journal of Food Technology (March)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Indonesia's natural resources are rich in biodiversity which is a very prospective potential to bedeveloped. One of them is palm tree. The main result of palm tree was sap. Palm sap can be processedinto sugar or vinegar. This study aims to know the effect of addition of bread yeast and fermentationtime on the characteristics of vinegar, determine the best treatment of adding bread yeast and fermentation time to produce vinegar from sap palm (Borassus flabellifer Linn), and to know the stabilityof vinegar products for storage temperature. The experimental design used was factorial CompletelyRandomized Design with 2 factors, namely the addition of bread yeast (0%, 1%, 2%, 3%, 4% and 5%)and fermentation time (0, 10, 15, 20, 25 and 30 days). The data obtained were analyzed by ANOVA andif the treatment had a significant effect it was followed by the Duncan test. The results showed that (1)the treatment of adding bread yeast had an effect on acetate levels, alcohol content, total soluble solidsand pH; but does not affect total sugar; fermentation time affects the level of acetic acid, total sugar,alcohol content, total soluble solids and pH; while the interaction between treatments has an effect ontotal soluble solids and pH, but does not affect acetic acid levels, total sugar and alcohol content onvinegar from fermented of palm sap, (2) treatment of addition of 5% bread yeast with 30 daysfermentation time was the best treatment for producing vinegar, with characteristics of acetic acid levelof 2.72% v / v, total sugar 0.03%, alcohol content 0.68% and total soluble solids 2.60 °Brix; (3) thestability of vinegar products produced at various storage temperatures an affects the levels of aceticacid and total soluble solids.
Aplikasi Pati Talas Kimpul Termodifikasi Secara HMT (Heat Moisture Treatment) Pada Pembuatan Bakso Ayam I Putu Suparthana; I Nengah Kencana Putra; Ni Wayan Wisaniyasa
Media Ilmiah Teknologi Pangan (Scientific Journal of Food Technology) Vol 3 No 2 (2016)
Publisher : Master Program of Food Science and Technology, Faculty of Agricultural Technology, Udayana University

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Abstract

Pengenyal merupakan bahan yang sangat penting dalam pembuatan bakso ayam karena dapat menghasilkan bakso dengan karakteristik kenyal, kompak dan juicy. Hingga saat ini, bahan pengenyal yang banyak dan umum digunakan adalah sodium tri poli fosfat (STPP). STPP merupakan bahan tambahan pangan sintetik, yang bila digunakan secara berlebihan akan beresiko pada kesehatan. Disis lain pati talas kimpul termodifikasi (PTKT) yang merupakan pati tipe C memiliki daya ikat air tinggi, stabilitas terhadap panas yang baik, dan kemampuan membentuk gel yang tinggi. Sifat-sifat ini dapat digunakan sebagai pengenyal alami menggantikan STTP. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan PTKT terhadap karakteristik bakso ayam, pengaruh jumlah penambahan PTKT yang optimal pada proses pembuatan bakso ayam, dan mengetahui karakteristik bakso ayam yang ditambahkan PTKT. Disain penelitian menggunakan rancangan acak kelompok dengan perlakuan berupa penambahan PTKT yang dilakukan dalam enam taraf (0,5,10,15,20 dan 25%) dan diulang sebanyak tiga kali. Data dianalisis dengan sidik ragam dan perbedaan antar nilai rata-rata diuji dengan Duncan’s multiple range test. Data kadar air tertinggi didapat pada taraf perlakuan 20%. Hasil analisis sensori menunjukkan adanya pengaruh pada karakteristik (aroma dan citarasa) bakso ayam. Jumlah penambahan PTKT yang paling disukai sebanyak 10% dan masih dapat diterima hingga 15%.
Sifat Fisik, Kimia, dan Sensori Mi Instant yang Terbuat dari Tepung Komposit Terigu dan Pati Kimpul Modifikasi I Nengah Kencana Putra; I Putu Suparthana; Anak Agung Istri Sri Wiadnyani
Jurnal Aplikasi Teknologi Pangan Vol 8, No 4 (2019): November 2019
Publisher : Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.856 KB) | DOI: 10.17728/jatp.5161

Abstract

Pati kimpul modifikasi (PKM) merupakan pati yang dibuat dari umbi kimpul yang diberikan perlakuan fisik atau kimia sehingga mempunyai sifat fungsional lebih baik dibandingkan dengan pati kimpul alami. Pada penelitian ini, dievaluasi pengaruh komposisi tepung komposit (terigu-PKM) terhadap sifat fisik, kimia, dan sensori mi instant. PKM dibuat menggunakan metode high moisture treatment (HMT), yaitu pemanasan pati pada suhu 110˚C pada kadar air 30% selama 10 jam. Tepung komposit dibuat dengan menggunakan berbagai perbandingan terigu dan PKM (90:10, 80:20, 70:30, 60:40, 50, dan 50). Selanjutnya, tepung komposit ini diolah menjadi mi instan, dan kemudian sifat fisik, kimia, dan sensori mi instan yang dihasilkan dievaluasi. Hasil penelitian menunjukkan perbandingan terigu dan PKM pada tepung komposit berpengaruh nyata terhadap kadar air, kadar protein, kadar karbohidrat, waktu pemasakan, kehilangan padatan akibat pemasakan (KPAP), dan daya serap air mi instan. Berdasarkan hasil uji sensori, tepung komposit terigu-PKM (80:20) menghasilkan mi instan terbaik. Komposisi zat gizi mi instan yang dihasilkan adalah: kadar air (2,74%), lemak (29,19%), protein (7,68%), dan karbohidrat (58,91%). Mi instan terigu-PKM (80:20) memiliki aroma lebih baik, waktu pemasakan lebih singkat, dan daya serap air lebih baik dibandingkan mi terigu 100%, namum memiliki KPAP lebih tinggi. Kesimpulannya, PKM dapat digunakan sebagai pensubstitusi sebagian terigu dalam pembuatan mi instant.Physical, Chemical, and Sensory Properties of Instant Noodles Prepared from Wheat - Modified Tannia Starch CompositeAbstractModified tannia starch (MTS) is the starch made from tannia tuber, which is provided a physical or chemical treatment so that it has better functional properties compared to native tannia starch. In this study, the effect of the composition of the wheat flour-MTS composite on the physical, chemical, and sensory properties of instant noodles was evaluated. MTS was produced by using the high moisture treatment (HMT) method, which was heating the starch at the temperature of 110˚C and moisture content of 30% for 10 hours. The composite flour was made with various ratios of wheat flour and MTS (90:10, 80:20, 70:30, 60:40, 50, and 50). Subsequently, the composite flour was processed into instant noodles, and then the physical, chemical, and sensory properties of the noodles produced were evaluated. The results showed the ratio of wheat flour and MTS had a significant effect on the water content, protein content, carbohydrate content, cooking time, cooking loss, and water absorption capacity of the instant noodle. Based on the results of sensory evaluation, the composite of wheat flour-MTS (80:20) could produce the best instant noodles. The nutrition composition of the instant noodles produced, namely: water content (2.74%), fat content (29.19%), protein content (7.68%), and carbohydrate content (58.91%). If compared to the 100% wheat flour instant noodles, the wheat flour-MTS (80:20) instant noodles has a better aroma, better water absorption capacity, and shorter cooking time, but it has a higher cooking loss. In conclusion, MTS can be used as a partial substitute for flour in making instant noodles.
Analisis sensori dan gizi kerupuk sagu kaya nutrisi dari ulat sagu (Rhyinchophorus ferrugineus) I Putu Suparthana; Adina Juniati Vortina Panggabean; Ni Made Yusa
AGROINTEK Vol 17, No 4 (2023)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v17i4.17084

Abstract

Kerupuk is a populist cracker in Indonesia. Its production in this study is aimed to promote kerupuk to become a healthy snack food by utilizing the local protein source namely the larva of Rhynchophorus ferrugineus which is processed into flour. The proportion of Sagu flour and larva flour as the main part of the dough was studied to find the one which resulted in the best product. The experiment was conducted as a Randomized block designed with five level treatments namely the ratio of 100:0; 90:10; 80:20; 70:30; and 60:40. Sensory and nutrient parameters data were analyzed by using analysis of variance tool. The panelists accepted the product resulting from the dough with the ratio of 90 % sagu flour and 10 % larva flour. It contains of 8.72 % of water, 1.65 % of ash, 5.25 % of protein, 0.94 % of fat, 82.20 % of carbohydrates and the swellability is 10.60 %. It was concluded that the 90 %:10 % proportion of sagu flour and larva flour resulted in the best product.
Rebranding Keripik Pelepah Pisang Produk UP2K PKK Desa Kukuh Kerambitan Tabanan Julianto, I Nyoman Larry; Mudra, I Wayan; Suparthana, I Putu; Adnyana, I Nyoman Widhi; Andreani, Ni Putu Elvian
Wikrama Parahita : Jurnal Pengabdian Masyarakat Vol. 8 No. 1 (2024): Mei 2024
Publisher : Universitas Serang Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30656/jpmwp.v8i1.5512

Abstract

Tujuan kegiatan pengabdian adalah pengembangan peningkatan kualitas desain kemasan dan strategi pemasaran keripik pelepah pisang kelompok Usaha Peningkatan Pendapatan Keluarga (UP2K) PKK Desa Kukuh Kerambitan. Kegiatan pengabdian dilaksanakan menggunakan dua tahap yaitu tahap Focus Group Discussion (FGD) untuk penentuan nama produk beserta elemen visual produk dan workshop strategi pemasaran digital melalui pembuatan desain poster digital. Hasil FGD menyepakati nama ‘DèBong’ sebagai identitas produk keripik yang cenderung mampu mewakili bahan bakunya, yakni pelepah pisang atau gedebong. Referensi terkait Ilustrasi dan warna yang disepakati adalah berdasarkan warna alami pada pelepah pisangnya serta unsur lainnya pada pohon pisang, seperti daun dan buahnya. Kegiatan PKM mendapatkan apresiasi yang sangat baik dari Ketua Tim Penggerak Pemberdayaan dan Kesejahteraan Keluarga (TP–PKK) dan Kepala Desa Kukuh Kerambitan. Capaian kegiatan yang dilaksanakan mencapai di angka 120%, yakni pada tingkat pengetahuan dan keterampilan peserta pendampingan dalam memahami dan mengkonsepkan desain kemasan serta strategi promosi melalui media sosial menjadi lebih meningkat.
Strategi Pemasaran Kripik Pelepah Pisang Dèbong Melalui Instagram Julianto, I Nyoman Larry; Mudra, I Wayan; Suparthana, I Putu; Permadi, Giovanni Brewijaya Putra; Andreani, Ni Putu Elvian
Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Vol 6, No 1 (2023): Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstormin
Publisher : Politeknik Harapan Bersama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30591/japhb.v6i1.4390

Abstract

Tim Penggerak Pemberdayaan dan Kesejahteraan Keluarga merupakan organisasi kemasyarakatan yang memberdayakan perempuan untuk berpartisipasi dalam pembangunan di Desa. Salah satu Program PKK adalah berupaya meningkatkan kelompok dan kualitas Usaha Peningkatan Pendapatan Keluarga (UP2K) PKK. Mendukung perihal tersebut, maka pihak Desa bekerjasama dengan UPTD LLK Kabupaten Tabanan mengadakan Pelatihan Keterampilan Kerja Berbasis Kompetensi tentang pembuatan kripik pelepah pisang. Pasca kegiatan pelatihan tersebut, mengakibatkan kelompok UP2K PKK (Mitra PKM) memiliki permasalahan lanjutan, yakni adanya keterbatasan kemampuan dalam mendesain media promosi untuk pemasaran produknya. Tujuan kegiatan pengabdian ini adalah meningkatkan kemampuan mendesain media promosi yang sesuai dengan karakteristik khalayak sasaran. Metode workshop yang diawali dengan FGD untuk penentuan elemen desain, dilaksanakan sebagai tahapan melatih kemampuan Mitra dalam mendesain poster digital sebagai media promosi dengan aplikasi Canva. Kegiatan ini menghasilkan peningkatan kemampuan mendesain poster digital untuk meningkatkan strategi promosi DèBong pada media sosial berupa instagram dalam upaya meningkatkan brand value produk.
Inventarisation and Study of Sere kedele Quality in Local Market in Gianyar Regency Made Dwi Widyantari; I Putu Suparthana; I D. G. Mayun Permana
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol. 2 No. 2 (2017): Oktober
Publisher : Fakultas Teknologi Pertanian, Universitas Udayana Gedung GA, Fakultas Teknologi Pertanian, Universitas Udayana Jl. Raya Bukit Jimbaran, Jimbaran, Kuta Selatan, Bali Telp/Fax: (0361) 701801

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA.2017.v02.i02.p03

Abstract

The aims of this research was to determine and inventory diversity of sere kedele production process traditionally in Gianyar Regency and to know the different of nutritional aspect on sere kedele produced. This research was conducted in 2 stages. The first stage is a survey to obtain information on materials and production equipment and sere kedele processing. The data obtained are tabulated and presented descriptively. The second stage is the analysis of nutritional components of sere kedele that includes water content, ash content, protein content, fat content and carbohydrate content. The results of the research in the first stage shows that in Gianyar Regency there are 3 subdistricts that produce sere kedele Sukawati, Gianyar and Blahbatuh, there are variations in the processing method used include boiling duration, fermentation length, spice addition technique before and after fermentation and fermentation container used closed and somewhat open. The results of the second stage of research showed that the nutritional components of sere kedele includes water content ranges from 56.89% to 64.65%, ash content ranges from 1.56% to 2.42%, protein content ranges from 14.90% to 20.93%, fat content ranges between 5.34% to 9.50% and carbohydrate content ranges from 10.81.% to 12.97%.
Kajian Asam Amino pada Fermentasi Talas (Colocasia esculenta L. Schott) Trisna Darmayanti; IDG. Mayun Permana; AAGN Anom Jambe; AAI Sri Wiadnyani; IP Suparthana; IDP Kartika Pratiwi
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol. 2 No. 1 (2017): April
Publisher : Fakultas Teknologi Pertanian, Universitas Udayana Gedung GA, Fakultas Teknologi Pertanian, Universitas Udayana Jl. Raya Bukit Jimbaran, Jimbaran, Kuta Selatan, Bali Telp/Fax: (0361) 701801

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA/2017.v02.i01.p04

Abstract

Penelitian ini bertujuan untuk mengetahui kadar total protein serta jenis asam amino yang terdapat pada tape talas (fermentasi talas). Pengukuran kadar protein dilakukan dengan menggunakan metode Kjeldahl, sedangkan kadar asam-asam amino dianalisis dengan menggunakan peralatan High Performance Liquid Chromatography. Hasil penelitian menunjukkan bahwa terjadi peningkatan kadar protein dari talas sebelum difermentasi (talas kukus) sebesar 0,767% menjadi 0,870% setelah difermentasi (talas kukus). Pada tape talas terkandung sebanyak 13 asam amino, yaitu asam aspartat, asam glutamat, serin, histidin, glisin, treonin, arginin, alanin, tirosin, valin, fenilalanin, isoleusin, dan lisin dimana 4 diantaranya merupakan asam-asam amino esensial. Dengan menggunakan uji t-student dari pasangan, yaitu talas sebelum dan sesudah difermentasi diketahui bahwa terdapat perbedaan antara kadar protein dan asam-asam amino pada kedua perlakuan. Talas yang difermentasi menjadi tape talas cenderung memiliki kadar protein dan asam-asam amino yang lebih tinggi dibandingkan dengan talas yang tidak difermentasi (talas kukus)
Pengaruh Konsentrasi Ragi Rhizopus oligosporus DP02 BALI Terhadap Karakteristik Tempe Kedelai (Glycine max (L) Merrill) Saraswati Permatasari, Ni Gusti Ayu; Suparthana, I Putu; Wisaniyasa, Ni Wayan
Itepa : Jurnal Ilmu dan Teknologi Pangan Vol. 14 No. 3 (2025): ITEPA
Publisher : Study Program of Food Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/itepa.2025.v14.i03.p02

Abstract

Abstract Tempe is a fermented soybeans or other ingredients, utilizing various types of yeast including Rhizopus oligosporus and Rhizopus oryzae. This study specifically employed Rhizopus oligosporus DP02, which was derived from an inoculum and isolated from hibiscus leaves. The purpose of the research was to determine the impact of different concentrations of Rhizopus oligosporus DP02 Bali on the characteristics of soybean tempeh and to identify the optimal yeast concentration for producing tempeh with the best qualities. The experimental design used was a completely randomized design featuring five levels of yeast concentration: 1%, 2%, 3%, 4%, and 5%. Each treatment was replicated three times, resulting in 15 experimental units. The data were analyzed using analysis of variance and if a significant effect was found, further testing would be conducted using the Duncan Multiple Range Test. The results indicated that yeast concentration significantly affected water content, ash content, protein content, fat content, carbohydrate content, color, taste, texture, and overall acceptance, but did not influence aroma. The findings revealed that a 4% yeast concentration yielded soybean tempeh with a water content of 38.09%, 1.15% for ash content, 17.68% for protein content, 17.18% for fat content, and 25.71% for carbohydrate content. Additionally, sensory tests for color, smell, taste, texture, and overall acceptance were positively received by the panelists. Keywords: Tempeh, soya bean, yeast, concentration, Rhizopus oligosporus Abstrak Tempe merupakan hasil fermentasi dari kacang kedelai ataupun bahan lain dengan memanfaatkan bermacam variasi kapang antara lain Rhizopus oligosporus dan Rhizopus oryzae. Penelitian ini scara khusus menggunakan Rhizopus oligosporus DP02 yang berasal dari inokulum dan diisolasi dari daun waru. Tujuan penelitian ini guna mengetahui dampak konsentrasi Rhizopus oligosporus DP02 Bali berbeda di karakteristik tempe kedelai serta untuk mengetahui konsentrasi ragi maksimal demi hasil tempe berkualitas terbaik. Desain percobaan terpakai ialah desain acak lengkap berlima taraf konsentrasi ragi yaitu 1%, 2%, 3%, 4%, dan 5%. Pengulangan perlakuan tiga kali hingga didapat 15 satuan percobaan. Data dianalisa dengan analisis variasi ANOVA serta bila terdapat pengaruh akan diuji lanjutan memakai uji jarak berganda Duncan. Hasil penelitian memperlihatkan konsentrasi ragi berdampak aktual ke kadar air, kadar abu, kadar protein, kadar lemak, kadar karbohidrat, warna, rasa, tekstur, serta akseptansi total namun tidak berdampak ke aroma. Hasil penelitian memperlihatkan konsentrasi ragi 4% menciptakan tempe kedelai berkarakteristik unggul yakni berkadar air 38,09%, 1,15%, kadar protein 17,68%, dan kadar karbohidrat 25,71%. Uji sensori warna, aroma, rasa, tekstur, serta penerimaan total diterima secara positif oleh panelis. Kata Kunci: Tempe, kacang kedelai, konsentrasi, Rhizopus oligosporus
Co-Authors AA. Devi Pradnyanita AAGN Anom Jambe Adina Juniati Vortina Panggabean Adina Juniati Vortina Panggabean Agus Selamet Duniaji Amira Putri Anak Agung Istri Sri Wiadnyani Andreani, Ni Putu Elvian Anjani Fatnasari Antonius Solo Asri Andriani Betari Nurrachmah Kusuma Putri Desak Nyoman Anggreni Bhaskara Dewi Citra Laksmi Agung Putri Elia Agatha Christiani Elicia Debora Marbun, Monica Elsa Fanora Sinaga Enrica Balanzi, Dhenabe Flora Anggreni Sitinjak G.P. Ganda Putra Gusti Ayu Kadek Diah Puspawati Hana Kusuma Brahmasari Hossiana Hariputeri I D. G. Mayun Permana I D.P. Kartika Pratiwi I Dewa Gde Mayun Permana I Ketut Suter I Made Adhi Dharma Parayana I Made Sugitha I Nengah Kencana Putra I Nyoman Larry Julianto I Nyoman Widhi Adnyana I Wayan Mudra, I Wayan Icha Nabillah IDG. Mayun Permana IGusti Ngurah Agung Iis Sholin Ketut Ayurina Singarsari Diasaputri KOMANG AYU NOCIANITRI Komang Ayu Puspa Hapsari Komang Triani Utami L. Paramita Liya Paramita Lucia Amitasya Sinaga Luh Komang Puspa Handayani Luh Putu Trisna Darmayanti Made Dwi Widyantari Masahiro Nogawa Masahiro Nogawa Masayuki Hashimoto Mia Primsa Ginting Mineo Kojima Mineo Kojima N.M. Yudantin Nathania Pusparani Nur Ni Komang Tri Handayani Ni Luh Cintya Febriani Ni Made Indri Hapsari Arihantana Ni Made Inten Kusuma Dewi Ni Made Susi Kartika Ni Made Wartini Ni Made Yudantini Ni Made Yusa Ni Made Yusa Ni Nyoman Budiasih Ni Nyoman Puspawati Ni Wayan Wisaniyasa Nyoman Geatrin Arumsari Pandu Prayugo Paramita Wulandari, Kadek Ratih Permadi, Giovanni Brewijaya Putra Pinky Natalia Samanta Putri Irdayanti Putri Nathasya br Lahi Putu Timur Ina Putu Yumiko Murdiasa Ricky Suranta Perangin-angin Saraswati Permatasari, Ni Gusti Ayu Sayi Hatiningsih Sayi Hatiningsih Sutriswati Rahayu, Endang Tsutomu Shimizu