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All Journal HAYATI Journal of Biosciences Jurnal Sain Veteriner Buletin Peternakan Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Jurnal Kedokteran Brawijaya Jurnal Biologi Tropis Jurnal Ilmu Alam dan Lingkungan JURNAL KAJIAN VETERINER BIOEDUPAT: Pattimura Journal of Biology and Learning Berkala Penelitian Hayati Biofaal Journal TROPICAL GENETICS Pakem : Jurnal Pengabdian Kepada Masyarakat Tropical Genetics BAKTI : Jurnal Pengabdian Kepada Masyarakat
Pieter Kakisina
Jurusan Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Pattimura, Ambon
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Efek Terapi Ekstrak Etanol Sirih Cina (Peperomia pellucida L.) terhadap Peningkatan Konsentrasi dan Motilitas Spermatozoa Tikus (Rattus norvegicus) Terpapar Sopi Marlyn Wattimena; Pieter Kakisina; Adrien Jems Akiles Unitly
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi Vol 15, No 1 (2023): Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jbe.v15i1.32717

Abstract

Penelitian ini bertujuan untuk mengetahui efek terapi ekstrak etanol sirih cina (Peperomia pellucida L.)  terhadap peningkatan konsentrasi dan motilitas spermatozoa tikus Rattus norvegicus terpapar sopi. Penelitian ini menggunakan rancangan acak lengkap (RAL), dimana 15 ekor tikus Rattus norvegicus dibagi dalam 5 kelompok perlakuaan dengan 3 kali ulangan, yaitu K(-):Kelompok kontrol negatif yaitu kelompok tikus yang diberi sopi 5.4 ml/ekor/hari selama 14 hari, K(+):Kelompok kontrol positif yaitu kelompok tikus yang diberi sopi 5.4 ml/ekor/hari selama 14 hari kemudian vitamin C 3.2 mg/kg BB selama 14 hari, P1: Kelompok tikus yang diberi sopi 5.4 ml/ekor/hari selama 14 hari kemudian diberi ekstrak etanol sirih cina 0.36 g/kg BB selama 14 hari, P2: Kelompok tikus yang diberi sopi 5.4 ml/ekor/hari selama 14 hari kemudian diberi ekstrak etanol sirih cina 0.72 g/kg BB selama 14 hari dan P3: Kelompok tikus yang diberi sopi 5.4 ml/ekor/hari selama 14 hari kemudian diberi ekstrak etanol sirih cina 1.44 g/kg BB selama 14 hari. Hasil yang diperoleh dianalisis dengan Analysis of Variance (ANOVA) dan dilanjutkan dengan uji duncan pada tarif nyata  = 0.05 menggunakan perangkat lunak SAS dan dilanjutkan dengan uji beda nyata terkecil untuk mengetahui perbedaan perlakuan yang diberikan. Hasil penelitian menunjukkan bahwa ekstrak etanol sirih cina (Peperomia pellucida L.) berpengaruh terhadap peningkatan konsentrasi dan motilitas spermatozoa tikus Rattus norvegicus terpapar sopi, dengan dosis efektif adalah 1.44 gr/kg BB.
The Effect of Eucheuma cottonii Extract on Body Weight and Blood Sugar Levels of Mouse (Mus musculus) Diabetes Mellitus Type 1 Abdu Mahid Ukratalo; Pieter Kakisina; Meigy N Mailoa
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.4712

Abstract

Seaweed (E. cottonii) is a type of seaweed from the Rhodophyta class which has anti-diabetic activity so that it can control diabetes mellitus. The purpose of this study was to determine the effect of E. cottonii extract on body weight and blood sugar levels in mice (Mus musculus) type 1 diabetes mellitus. This study used a completely randomized factorial design with five treatments and three replications. 20 mice were divided into 5 groups: normal mice (KN), negative control (K-), positive control (K+), E. cottonii extract dose of 0.4 ml/g BW (P1) and E. cottonii extract dose of 0 .7 ml/gBB (P2). The extraction of E. cottonii used the maceration method with 96% ethanol solvent. All groups of mice were induced with 0.3 ml of STZ for 3 consecutive days. After an increase in blood glucose levels, the mice in the K(+) group were given metformin at a dose of 0.4 ml, P1 was given E. cottonii ethanol extract at a dose of 0.4 ml/g BW and P2 at a dose of 0.7 ml/g BW. Extract administration was carried out for 7 days. The results showed that E. cottonii extract had an effect on increasing body weight and decreasing blood sugar levels in type 1 diabetes mellitus mice. The increase in body weight and decrease in blood sugar levels was in line with the high doses given.
Mapping and distribution of the telomeric sequences (T2AG3 repeats) in the marsupialia famili Macropodidae Rony Kunda; Mechiavel Moniharapon; Pieter Kakisina
Tropical Genetics Vol. 1 No. 2 (2021)
Publisher : Genetikawan Muda Indonesia

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Abstract

Marsupial and monotreme mammals very important in vertebrate phylogeny studies between reptile-mammal divergence 310 million years ago (mya) and the eutherian (placental) mammal radiation 105 mya. They have many features including their distinctive chromosomes, which in marsupials are typically very large and well conserved between species. Monotreme genomes are divided into several large chromosomes and many smaller chromosomes, with a complicated sex chromosome models that forms a translocation chain in male meiosis. In Macropus genera members, telomeres have been shown to be involved in the fusion and inversion of chromosomes, but for Kangaroo, the telomere sequence remains consistent and does not change during the chromosomal fusion event, but this is not the case in mice. The ancestor of marsupials (plesiomorphic), has a karyotype of 2n=14, but for kangaroos and wallabies have a karyotype of 2n=22, based on fews curently research, we asserted that this karyotype is derived from the karyotype of marsupial ancestors who have a karyotype of 2n=14. Based on molecular genetic studies have shown that W. bicolor (swamp wallaby) is more appropriately grouped into the Macropus genera, not as a “sister” of Macropus genera. In addition Macropus and W. bicolor there has been a division of chromosomes due to chromosome fusion, but W. bicolor has fewer chromosomes than other members of Macropodidae, i.e (2n=10 for females, and 2n=11 for males).
Keanekaragaman dan Dominansi Vegetasi Pohon Pada Habitat Eulipoa wallacei di Hutan Hii Desa Haruku Kabupaten Maluku Tengah Picarima, Reynita; Kakisina, Pieter; Sahertian, Dece Elisabeth
Jurnal Ilmu Alam dan Lingkungan Vol. 15 No. 2 (2024): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

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Abstract

The Hii Forest of Haruku Village is where Eulipoa wallacei lays eggs. The Hii Forest is overgrown with tree vegetation that Eulipoa wallacei uses as a perch, a place to forage for food and to protect itself from predators. This study aimed to determine the diversity and dominance of tree vegetation in the habitat of Eulipoa wallacei in the forest. The method used in this study is the quadratic method by placing sample plots randomly based on public information about the location of Eulipoa wallacei. The results showed 29 plant species belonging to 2 classes, 13 orders, and 22 families. The Diversity Index and Dominance Index of tree vegetation in the habitat of Eulipoa wallacei in Hii Forest showed diversity in the medium category and dominance in the low category. Eulipoa wallacei's habitat is used for perching, foraging, and protecting itself from predators.
PENERAPAN TEKNOLOGI PEMBUATAN PUPUK CAIR RAMAH LINGKUNGAN DARI LIMBAH KULIT PISANG DAN NANAS MENGGUNAKAN AKTIVATOR EM4 Kunda, Rony Marsyal; Kakisina, Pieter; Salamor, Reinhard; Manuhutu, Fredrik; Lakoan, Milda Rianty
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 5 No 2 (2025): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pakem.5.2.47-54

Abstract

Household organic waste such as banana and pineapple peels are often not optimally utilized, even though they contain nutrients that are good for plants. This community service activity aims to provide counseling and training on making environmentally friendly liquid fertilizer by utilizing banana and pineapple peel waste using EM4 activator to the community in Kamal Village, West Seram Regency (SBB), Maluku Province. Through a participatory approach, this activity involves the community directly in the process of making liquid fertilizer, starting from the preparation of ingredients, fermentation, to application to plants through foliar spraying and direct watering to the planting media with a measured dose (50-100 ml of liquid fertilizer / 1 liter of water) every 7 days. This activity was carried out in 2 stages including the extension and training stages, as well as the monitoring and evaluation stages. The results of the activity showed an increase in participants' knowledge and skills in managing organic waste into useful and environmentally friendly products. With this activity, it is hoped that the community can apply this simple technology independently to support sustainable agriculture and reduce environmental pollution
Penguatan Pengetahuan Masyarakat Desa Allang Asaude, Kabupaten Seram Bagian Barat tentang Tumbuhan Berkhasiat Obat Tuhumuri, Evelin; Kaihena, Martha; Ukratalo, Abdul M; Mainassy, Meilissa C; Kakisina, Pieter; Pagaya, Joseph; Seumahu, Cecelia Anna; Killay, Amos
Bakti : Jurnal Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2025): Bakti: Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Layanan Pendidikan Tinggi (LLDIKTI) Wilayah XII Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51135/bakti.5.1.1-9

Abstract

Desa Allang Asaude, yang terletak di Kabupaten Seram Bagian Barat, merupakan daerah yang kaya akan sumber daya alam, terutama keanekaragaman tumbuhan. Masyarakat setempat telah lama memanfaatkan berbagai jenis tumbuhan untuk kebutuhan sehari-hari, khususnya dalam pengobatan tradisional. Kegiatan pengabdian masyarakat ini dilaksanakan dengan tujuan untuk memberikan informasi dan pengetahuan kepada masyarakat Desa Allang Asaude tentang berbagai tumbuhan yang memiliki khasiat sebagai obat. Kegiatan ini dilaksanakan pada hari Sabtu, 11 November 2024. Metode yang digunakan adalah penyuluhan langsung kepada masyarakat melalui ceramah dan diskusi interaktif. Hasil kegiatan menunjukkan bahwa materi yang disampaikan sangat bermanfaat dan berhasil meningkatkan pengetahuan masyarakat mengenai berbagai tumbuhan obat yang ada di sekitar mereka. Masyarakat kini lebih memahami cara pengolahan yang benar dan cara pemanfaatan tumbuhan untuk mengatasi penyakit ringan, seperti demam, batuk, dan gangguan pencernaan. Selain itu, kegiatan ini juga meningkatkan kesadaran masyarakat akan pentingnya melestarikan tumbuhan obat sebagai bagian dari warisan budaya dan sumber daya alam yang perlu dijaga agar dapat dimanfaatkan oleh generasi mendatang.
Co-Authors Abdu Mahid Ukratalo Adrien Jems Akiles Unitly Amos Killay Bambang Poernomo Chomsa Ditasari Umi Baszary Baszary Eifan Boyke Pattiasina Ferymon Mahulette Fredrik Manuhutu, Fredrik Hasan Tuaputty Healthy Kainama Lakoan, Milda Rianty Linda Linda Mainassy, Meilissa C Maman Rumanta Mardi Santoso Marlyn Wattimena Martha Kaihena Marthinus Usmany Marthinus Usmany Meigy N Mailoa Moniharapon, Mechiavel Pagaya, Joseph Picarima, Reynita Rasyad Indra Rony Kunda Rony Marsyal Kunda Sahertian, Dece Elisabeth Salamor, Reinhard Seumahu, Cecelia Anna Slamet Diah Volkandari Sri Fatmawati Taslim Ersam Tuhumuri, Evelin Ukratalo, Abdul M Win Darmanto