Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : The Journal of Pure and Applied Chemistry Research

Modulation of Perlecan Protein towards Chondrocyte Secretion Factors at the Articular Cartilage in Hyperglycemic Animal Model Ibrahim Njoto; Fatchiyah Fatchiyah; Kusworini Handono; Abdurrachman Abdurrachman; Djoko W. Soeatmadji; Handono Kalim
The Journal of Pure and Applied Chemistry Research Vol 8, No 1 (2019): Edition January-April 2019
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2019.008.01.450

Abstract

High carbohydrate influences the cartilage microcellular environment and chondrocytes. Perlecan (HSPG2) conducts an essential role as chondrocytes mechano-transducer and chondrocytes secretion factors. This research aims to fulfill the research gap about hyperglycemia which influences to perlecan of articular cartilage. About twenty male rats were divided into four groups: group I (sugar-treated rats 1.00 mg/kg of BW), group II (sugar-treated rats 1.5 mg/kg of BW), group III (2.00 mg/kg of BW), and control. The Anterior Cruciate Ligament Transection (ACLT) used to generate an unstable joint to be osteoarthritis condition at the right knee. Then, sugar was administrated for two months.  Level expression of the protein was analyzed using Western Blotting. The result showed that perlecan expression was decreased within all sugar-treated rats group as compared to control. The IGF-1 expression was decreased, whereas TGF-β was slightly increased within all treatment groups. Moreover, the expression of FGF-2 was higher in all treatment groups. Of note, IL-1β expression was only elevated in group II and III. The interaction of perlecan to the chondrocyte secretion factors was determined by the cartilage condition within hyperglycemia.
Co-Authors A Rifa’i Abd. Rasyid Syamsuri Abdurrachman Abdurrachman Achmad Rifa’i, Achmad Achmad Rifa’i Achmad Rudijanto Agustina T Endharti, Agustina T Agustina Tri Endharti Aru W Sudoyo Asri Nugraheni Ati Rastini Atma Gunawan Atma Gunawan Bambang Setyohadi Benny A Pradana, Benny A BP Putra Suryana Dany Farida Deddy Nur Wachid Achadiono Desfryda, Elynca Putri desy wulandari Dewi Purnama Sari Dian Hasanah Djoko W. Soeatmadji Djoko Wahono S Dona Marisa, Dona Dwinadella, Sephia Eko, Mudjiwijono Handaru Elvira Sari Dewi Engli, Katherina Erawati, Dini Rachma fatchiyah . Fatchiyah Fatchiyah Ferdinandus Maria Judajana Fikriah, Ika Firdaningrum, Nimas Eka Hani Susanti, Hani Hani Susianti Hanik Ruliani Harry Isbagio Harun Al Rasyid Harun Al Rasyid I Putu A.S Ibrahim Njoto Ivan A Hartono, Ivan A Joewono Soeroso Joewono Soeroso Kusworini Handono Kusworini Handono Kusworini Handono Kusworini Handono Kusworini Kusworini Laksmi Karunia Tanuwijaya Laksmi Karunia Tanuwijaya Loeki Enggar Fitri M Aris Widodo Mat Suwito Moch Sasmito Djati Mudjiwijono Handaru Eko Muhammad Anshory Muhammad Anshory, Muhammad Muhammad Darwin P Nurdiana Nurdiana Perdana Aditya Rahman Pratama, Mirza Zaka Putri, Choirinnisa Meilia Ayu R.I R.I Rachmat Gunadi Wachjudi Radhitio A Nugroho, Radhitio A Respati Suryanto Dradjat Ria Famuji, Siti Roziah Rifa’i, A Rizky Fachry, Ade Wlidan Rooije R.H. Rumende Sari, Tita Luthfia Solly Aryza Sumarta, Norma Hanifah Supriono Supriono Suryana, Bagus Putu Putra Suwito, Mat Tamaulina Br Sembiring Torajasa Achamar Wahono, Caesarius Singgih Wahono, Cesarius Singgih Wisnu Barlianto Yona One Sidarta, Yona One Zaenal Kusuma