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Journal : The Indonesian Biomedical Journal

The Effect of Curcumin on Regression of Liver Fibrosis through Decreased Expression of Transforming Growth Factor-β1 (TGF-β1) Supriono Supriono; Asri Nugraheni; Handono Kalim; Mudjiwijono Handaru Eko
The Indonesian Biomedical Journal Vol 11, No 1 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i1.463

Abstract

BACKGROUND: Transforming growth factor (TGF)-β1 has a pivotal role in liver fibrogenesis. Curcumin effectively prevent the progression of liver fibrosis through inhibition of TGF-β1/Sma and drosophila MAD (Smad) signaling pathway. However, the role of curcumin in the regression of liver fibrosis is still unknown. This study investigated the role of curcumin and TGF-β1 in liver fibrosis regression.METHODS: An experimental Wistar rat model included 6 treatment groups as well as positive and negative control groups. The treatment and positive control groups were injected with carbon tetrachlorid (CCl4) for 9 weeks to induce liver fibrosis. After cessation of injection, 3 of the treatment groups were given curcumin and 3 were given carboxymethylcellulose (CMC) for 2, 5 and 9 weeks, while the positive control was untreated. The negative control was injected with normal saline. TGF-β1 liver tissue levels were analyzed by ELISA, while the TGF-β1 expression in liver cells was analyzed by immunohistochemical assay. The metavir score was used to assess the degree of liver fibrosis. Values of p<0.05 were regarded as statistically significant.RESULTS: Nine weeks of CCl4 injection induced liver fibrosis (metavir F3); and significantly increased TGF-β1 levels and expression in tissues (p=0.00, p=0.021, respectively). Curcumin administration decreased levels and expression of TGF-β1 in the liver and accelerated regression of liver fibrosis. There was a significant correlation between duration of administration of curcumin with an expression of TGF-β1 in the liver tissue (r=0.87; p<0.00).CONCLUSION: Curcumin accelerates regression of liver fibrosis, likely through decreasing of TGF-β1 expression in the liver.KEYWORDS: curcumin, TGF-β1, liver fibrosis regression, CCl4, animal model
Co-Authors A Rifa’i Abd. Rasyid Syamsuri Abdurrachman Abdurrachman Achmad Rifa’i, Achmad Achmad Rifa’i Achmad Rudijanto Agustina T Endharti, Agustina T Agustina Tri Endharti Aru W Sudoyo Asri Nugraheni Ati Rastini Atma Gunawan Atma Gunawan Bambang Setyohadi Benny A Pradana, Benny A BP Putra Suryana Dany Farida Deddy Nur Wachid Achadiono Desfryda, Elynca Putri desy wulandari Dewi Purnama Sari Dian Hasanah Djoko W. Soeatmadji Djoko Wahono S Dona Marisa, Dona Dwinadella, Sephia Eko, Mudjiwijono Handaru Elvira Sari Dewi Engli, Katherina Erawati, Dini Rachma fatchiyah . Fatchiyah Fatchiyah Ferdinandus Maria Judajana Fikriah, Ika Firdaningrum, Nimas Eka Hani Susanti, Hani Hani Susianti Hanik Ruliani Harry Isbagio Harun Al Rasyid Harun Al Rasyid I Putu A.S Ibrahim Njoto Ivan A Hartono, Ivan A Joewono Soeroso Joewono Soeroso Kusworini Handono Kusworini Handono Kusworini Handono Kusworini Handono Kusworini Kusworini Laksmi Karunia Tanuwijaya Laksmi Karunia Tanuwijaya Loeki Enggar Fitri M Aris Widodo Mat Suwito Moch Sasmito Djati Mudjiwijono Handaru Eko Muhammad Anshory Muhammad Anshory, Muhammad Muhammad Darwin P Nurdiana Nurdiana Perdana Aditya Rahman Pratama, Mirza Zaka Putri, Choirinnisa Meilia Ayu R.I R.I Rachmat Gunadi Wachjudi Radhitio A Nugroho, Radhitio A Respati Suryanto Dradjat Ria Famuji, Siti Roziah Rifa’i, A Rizky Fachry, Ade Wlidan Rooije R.H. Rumende Sari, Tita Luthfia Solly Aryza Sumarta, Norma Hanifah Supriono Supriono Suryana, Bagus Putu Putra Suwito, Mat Tamaulina Br Sembiring Torajasa Achamar Wahono, Caesarius Singgih Wahono, Cesarius Singgih Wisnu Barlianto Yona One Sidarta, Yona One Zaenal Kusuma