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All Journal HAYATI Journal of Biosciences Journal of Food and Pharmaceutical Science Reaktor Pharmaciana: Jurnal Kefarmasian Journal of Engineering and Technological Sciences Majalah Obat Tradisional Acta Pharmaceutica Indonesia Indonesian Journal of Chemistry Jurnal farmasi UIN Alauddin Makassar Current Research on Biosciences and Biotechnology JURNAL BIOLOGI PAPUA Journal of Food and Pharmaceutical Sciences
Muhamad Insanu
School of Pharmacy, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Earth & Planetary Sciences Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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Uji Aktivitas Antioksidan Tiga Spesies Tanaman Sarang Semut (Famili: Rubiaceae) Asal Kabupaten Merauke, Papua Septriyanto Dirgantara; As’ari Nawawi; Muhamad Insanu
Jurnal Biologi Papua Vol 5, No 1 (2013)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.52 KB) | DOI: 10.31957/jbp.517

Abstract

Sarang semut plant is one of indigenous medicinal plants of Merauke region, the Papua province. Local people use the bulb of sarang semut plants to cure inflammation, muscle pain and enhancing immunity. However rare research of the plants has been done especially on the antioxidant activity. The objective of this research was to investigate the antioxidant activity of three species of sarang semut plants which were Myrmecodia beccarii, Myrmecodia sp. and Hydnophytum sp. The test was necessary to identify the inhibitory concentration level of plant extract in blocking a free radical (IC50). Parts of plants were extracted with maceration using methanol for 3 x 24 h to produce concentrated methanol.  Plant extracts of three species were then subject to   antioxidant activity test based on free radical blocking of 1,1-Difenil-2-Pikrilhidrazil (DPPH) qualitatively and quantitatively using Ultraviolet-Visible spectrophotometry. The results showed the methanol extract of M. beccarii, Myrmecodia sp. and Hydnophytum sp. were active as antioxidants with IC50 from 8.18 ppm, 21.79 ppm, 25.31 ppm, respectively while Vitamin C as a control has IC50 7.85 ppm. In conclusion, three  plant species of sarangsemut are potential  as natural antioxidant and plant extracts of M. beccarii showed the highest antioxidant activity among others. Key words: Antioxidant, sarang semut plants, Rubiaceae, DPPH.  
UHPLC-Q-Orbitrap HRMS-based Untargeted Metabolomics of Sida rhombifolia Leaves and Stem Extracts Alfi Hudatul Karomah; Mohamad Rafi; Dewi Anggraini Septaningsih; Auliya Ilmiawati; Utami Dyah Syafitri; Nanik Siti Aminah; Muhamad Insanu; Abdul Rohman
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.770-778

Abstract

Sida rhombifolia, also known as sidaguri in Indonesia, is a medicinal plant commonly used as a herbal medicine because of its metabolite and biological activities. One of the several factors that affect plant metabolite composition and concentration is the use of plant parts. In this study, the experiment aimed to identify the metabolite profile in the leaves and stem extracts of S. rhombifolia using UHPLC-Q-Orbitrap HRMS-based untargeted metabolomics. The samples were distinguished by principal component analysis (PCA). Extraction of metabolites was conducted by sonication for approximately 30 min with 70% ethanol as the extraction solvent; 28 metabolites were identified. Seven metabolites were identified only in the leaves, three were identified only in the stems, and 18 other metabolites were identified in both the leaves and stems. These metabolites were categorized as flavonoids, triterpenoids, alkaloids, coumarins, phenolic aldehydes, phenolic acids, ecdysteroids, fatty acids, and monoterpene lactones. Based on the classification results, PCA grouped the leaves and stem extracts of S. rhombifolia using the peak area variables of the identified metabolites.
Uji Aktivitas Antioksidan dan Penetapan Kadar Flavonoid Total dan Fenolik Total Ekstrak Sidaguri (Sida rhombifolia L.) Asma Asma; Abdul Rohman; Djoko Santosa; Mohamad Rafi; Nanik Siti Aminah; Muhamad Insanu; Irnawati Irnawati
Journal of Food and Pharmaceutical Sciences Vol 10, No 2 (2022): J.Food.Pharm.Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.4955

Abstract

Sidaguri atau Sida rhombifolia L. telah digunakan oleh masyarakat sebagai obat tradisional, yaitu: penghilang rasa nyeri, radang, asam urat, penyakit kuning, muntah darah, dan sakit gigi. Faktor yang mempengaruhi produksi kandungan metabolit sekunder adalah kondisi lingkungan seperti tempat tumbuh, iklim, interaksi intra dan inter-spesifik serta waktu panen. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan serta kadar flavonoid dan fenolik total ekstrak sidaguri berdasarkan tempat tumbuh. Sampel diekstraksi dengan ultrasonic-assisted extraction (UAE) dan diuji penangkapan radikal bebas menggunakan metode 2,2’-difenil-1-pikrilhidrazil (DPPH) dan 2,2’-azino-bis(3-diethyl4benzothiazoline-6-sulfonic acid) (ABTS), serta penetapan kadar flavonoid total dan kandungan fenolik total. Hasil uji aktivitas antioksidan sidaguri dari Cangkringan dan Ngemplak memiliki aktivitas antioksidan tertinggi baik pada metode penangkapan radikal DPPH dengan nilai IC50 masing-masing sebesar 0,400±0,004 dan 0,403±0,004 mg/mL, maupun metode ABTS dengan nilai TEAC masing-masing sebesar 40,733±0,240 dan 35,598±0,153 mg/g serbuk sidaguri, dengan kadar flavonoid total masing-masing sebesar 10,095±0,068 dan 12,066±0,025 mg/g serbuk sidaguri dan kadar fenolik total masing-masing sebesar 56,45±0,068 dan 31,502±0,025 mg/g serbuk sidaguri.
Isolation of α-glucosidase enzyme inhibitor from titanus (Leea aequata L.) Bangar, Roy; Ningsih, Kurnia Nastira; Kartasasmita, Rahmana Emran; Insanu, Muhamad
Current Research on Biosciences and Biotechnology Vol. 6 No. 1 (2024)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2024.6.1/XCGSRS5W

Abstract

Diabetes mellitus is a metabolic disease characterized by high blood glucose levels. Indonesia was ranked fourth globally, with over 12 million people suffering from diabetes. Natural-based drugs can be an alternative for treating diabetes with fewer side effects and are expected to be more economical than conventional drugs. One of the plant families used to reduce blood glucose levels is the Vitaceae family. One of the Vitaceae species currently used for traditional medicine is Leea aequata. This study aimed to isolate active compounds with α-glucosidase inhibitor activity from titanus plant. This study began with collecting leaf, fruit, and stem samples from titanus plants that grow in North Sumatra. The extraction method used was maceration using ethanol 96%. The isolation stage started with fractionation, followed by purification with column chromatography, in which TLC monitored for each process. α-glucosidase in testing was carried out on extracts, fractions, and isolates using a reagent Multiscan microplate reader at 400 nm. Compound identification was done using spectrodensitometry and 1H-NMR spectroscopy. The IC50 value of the ethyl acetate fraction (2.4±0.02 µg/mL) was higher than the ethanol extract (8.96 ± 0.44 µg/mL), n-hexane fraction (21±0.61 µg/mL), and water fraction (16.96 ± 0.38 µg/mL), so it was continued to the isolation stage. The isolated compound was characterized as 3,3′,4′,5,7-Pentahydroxyflavone 3-rhamnoside (quercitrin) with the IC50 was 12.25 ± 0.2 µg/mL.
Parang Romang (Boehmeria virgata (Frost.) Guill.): Correlation of Phytochemistry with Antioxidant and Xanthine Oxidase Inhibitory Activities Arsul, Muhammad Ikhlas; Fidrianny, Irda; Insanu, Muhamad
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.457-464

Abstract

Secondary metabolites such as phenolic and flavonoid from the plant are essential in their activity, especially activities related to the prevention of oxidation and the inhibition of xanthine oxidase. One plant that is believed to have prevention of oxidation and XOI and correlates with secondary metabolites is parang romang. The purpose of this study is to assess the antioxidant capacity and xanthine oxidase inhibitory activities of parang romang, and to examine their relationship with the TPC and TFC. Total phenolic and flavonoid were measured for all parts, and the DPPH, FRAP, and CUPRAC methods determined antioxidant capacity. The activity of xanthine oxidase inhibition was also assessed. Flavonoids, alkaloids, and coumarin were found in parang romang. The steroid/triterpenoid was found in the stem, leaves, and flower; saponin was in the roots and flower; quinone and tannin were only in the roots. The root gave the highest of TPC, while the flower had the highest TFC. The highest antioxidant capacity was found in roots, leaves, flowers, and stems. The highest xanthine oxidase inhibitory was given by leaves (9.74±0.14 µg/ml), followed by flower, steam, and rood. The correlation was shown between the phenolic and flavonoid quantities and the actions of antioxidants and xanthine oxidase inhibitors.
Co-Authors Abdu Ravi Zakaria Abdul Rohman Abdul Rohman Abdul Rohman Abdul Rohman Akhirul Kahfi Syam Alfi Hudatul Karomah Anggra Paramita Anita Artarini Ardiyan Harimawan Ardiyan Harimawan Arsul, Muhammad Ikhlas Asefin Nurul Ikhtiarini Asep Gana Suganda Asma Asma As’ari Nawawi Auliya Ilmiawati Bangar, Roy Cindra Mutia Dadan Rohdiana Dianika Lestari Dianika Lestari Dianika Lestari Diky Mudhakir Dinilah, Nabila Pandu Djoko Santosa Dwi Rokhmat Setiawan Fakar Daras Kamal Ida Maryam Indra Wibowo Irda Fidrianny Irnawati Irnawati Irnawati Irnawati Kamal, Fakar Daras Khalisa Putri Aqilah Khalisa Putri Aqilah Komar Ruslan Komar Ruslan Wirasutisna Komar Ruslan Wirasutisna Komar Ruslan Wirasutisna Lia Marliani Maria Bintang Marliani, Lia Maryam, Ida Melia Laniwati Mohamad Rafi Mohamad Rafi Mohamad Rafi Mohamad Rafi Nabila Pandu Dinilah Nanik Siti Aminah Ningsih, Kurnia Nastira Nurma Sabila Rahmana Emran Kartasasmita Ruslan, Komar Salsafia Putri Salsafia Putri Sefni Reza Yolanda Selpida Handayani Septaningsih, Dewi Anggraini Setyaningsih, Widiastuti Shelly Shelly Sienny Wijaya Siti Kusmardiyani Suganda, Asep Gana Utami Dyah Syafitri Wijaya, Sienny Yohanes Andika Suharli