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Effect of Synthetic Fertilizer on Diazotrophic Bacteria in Secondary Forest and Oil Palm Soils in Central Kalimantan Agustina, Delia; Purnamasari, Maria Indah; Karmawan, Listya Utami; Suwanto, Antonius
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.106-116

Abstract

Soil microorganisms, especially diazotrophic bacteria, are vital for ecosystem sustainability, significantly contributing to nitrogen cycling and biodiversity conservation. Understanding the impact of different land-use practices on soil microorganisms, especially synthetic fertilizer addition, is essential for sustainable agriculture. This study compares soil properties, bacterial densities, and responses to fertilization in secondary forest soils and adjacent oil palm plantation soils located in PT Kerry Sawit Indonesia, Central Kalimantan. A greenhouse experiment was conducted using both secondary forest and oil palm plantation soils to evaluate the impacts of different synthetic fertilizers on nitrogen-fixing bacteria and soybean agronomic performance. Total soil bacteria and diazotrophic bacteria, essential for nitrogen fixation, were analyzed through microbiological assays and qPCR focusing on the nifH gene. Our findings show that oil palm plantation soils had higher levels of nitrogen, phosphate, and nitrate, while secondary forest soils had a higher abundance of diazotrophic bacteria. Furthermore, excessive nitrogen fertilization was found to reduce microbial density, negatively impacting plant sustainability, highlighting the importance of customized fertilizer management. The study highlights the intricate connections between land-use practices and microbial populations, providing valuable insights for balancing agricultural productivity with ecological sustainability.
Empowering PokTan ViCi 10 farmers in Ciomas Rahayu Village through hydroponic and aquaponic cultivation systems Karmawan, Listya Utami; Hutagalung, Rory Anthony; Yanti; Suryaatmadja, Irwin; Hardjawidjaja, Michelle Nadine; Wulandari, Yasinta Ratna Esti
Journal of Saintech Transfer Vol. 8 No. 1 (2025): Journal of Saintech Transfer
Publisher : Talenta Publisher Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jst.v8i1.18960

Abstract

The Villa Ciomas RW 10 Farmer Group (PokTan Vici 10), actively engaged in urban farming in Ciomas Rahayu Village, Bogor, West Java, has been utilizing a small plot of land located next to the Cisindangbarang River. Their urban farming area already includes a small fishpond but lacks aquaponics equipment. The community service team aims to enhance the utility of the urban farming facilities by installing aquaponics equipment, providing workshops on basic hydroponics and aquaponics knowledge, exploring its business potential, and offering hands-on hydroponics and aquaponics cultivation practice. Throughout the program, the team also conducts monitoring and guidance to ensure successful implementation and address any challenges encountered during the training program. The hydroponic/aquaponics equipment installation was successful; however, nutrient supply from the fishponds only was initially suboptimal, prompting the addition of hydroponic nutrients. Hydroponic techniques were also introduced for vegetable cultivation. Based on a questionnaire analysis conducted before and after the training, the program positively impacted participants, significantly enhancing their knowledge and aquaponic skills (Wilcoxon signed-rank test, p<0.05). In conclusion, the aquaponics equipment was successfully installed, and participants gained foundational knowledge and skills in aquaponics, enabling them to independently manage aquaponics systems through to harvest. Looking ahead, the community members plan to explore business opportunities in selling hydroponics/aquaponics products.
Genetic diversity analysis among Philodendron spp. by Random Amplified Polymorphic DNA Karmawan, Listya Utami; Gunawan, Renaldy
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.58061

Abstract

Variety development in ornamental plants increased rapidly due to the high demand during the COVID-19 outbreak. Therefore, analyzing the genetic diversity of ornamental plants is essential to assist breeders in improving and developing new cultivars with desirable traits. In this study, the genetic diversity of Philodendron species was analyzed. P. erubescens, P. 69686, P. burle-marx, P. lacerum, and P. micans were selected as the most popular species cultivated in Indonesian nurseries. These cultivars were assessed both genetically using Random Amplified Polymorphic DNA (RAPD) markers and morphologically. The genetic relationship among Philodendron spp. species was analyzed using DARwin 6.0 software, and a dendrogram was constructed based on the UPGMA algorithm. Based on the two selected RAPD primers, the five samples showed no clear genetic relatedness to each other, and the primers were insufficient to construct a single consensus dendrogram.
Lokakarya Berbasis Eksperimen sebagai Upaya Peningkatan Literasi Sains Siswa Sekolah Dasar: Pengenalan Konsep Indikator pH Alami Melalui Tinta Tak Terlihat Watumesa Agustina Tan; James Elliot Christanto; Ronald Kristianto Wibowo; Listya Utami Karmawan
Jurnal Pengabdian UNDIKMA Vol. 7 No. 1 (2026): February
Publisher : LPPM Universitas Pendidikan Mandalika (UNDIKMA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jpu.v7i1.17506

Abstract

This community service program aims to enhance elementary school students’ understanding of and interest in science through the implementation of interactive experiments based on the concept of natural pH indicators. The program was conducted at SD Athalia, Tangerang, involving 132 fifth-grade students who were divided into five groups of 26–27 students to ensure active participation. The implementation stages included an introduction to basic pH concepts, experimental demonstrations, and hands-on activities in which students created drawings using “invisible ink” made from a baking soda solution as a basic substance and turmeric as a natural pH indicator. The turmeric solution changed color from yellow to reddish-brown in areas that reacted with the base, revealing previously hidden images. The results indicate high levels of student enthusiasm, active participation, and curiosity toward science learning. These findings demonstrate that simple, activity-based experimental approaches are effective in fostering students’ interest in and initial understanding of science, while also representing a tangible contribution of higher education institutions to improving community science literacy.