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Journal : e-Journal Pustaka Kesehatan

Uji Aktivitas Antibakteri dan Antibiofilm Minyak Atsiri Rimpang Bengle (Zingiber purpureum Roscoe) terhadap Bakteri Staphylococcus epidermidis (Antibacterial and Antibiofilm Activity Test of Bengle Rhizome Essential Oil (Zingiber purpureum Roscoe) agains Hidayatul Ulyah; Evi Umayah Ulfa; Endah Puspitasari
Pustaka Kesehatan Vol 3 No 2 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Staphylococcus epidermidis has become one of the most important pathogenic bacteria that lead to nosocomial infection. This is caused by its ability to form a biofilm on the surface. Essential oils are one of the natural product that could be used as antiinfection agents. Bengle rhizome essential oil has been known to have activity against non-pathogenic bacteria S. epidermidis. This study was proposed to know the antibacterial and antibiofilm activity of bengle rhizome essential oil against clinical isolate of S. epidermidis at concentrations of 45, 60, 75, and 90%. Antibacterial activity was determined by the diffusion method with hole plate technique, while antibiofilm activity was determined by the microtiter assay method with crystal violet staining and quantified by measuring the optical density (OD) at λ 595 nm. This study showed that bengle rhizome essential oil has antibacterial and antibiofilm activity. The largest antibacterial and antibiofilm activity was shown from 90% concentration of bengle rhizome essential oil. Keywords: nosocomial infections, antibacterial, antibiofilm, bengle essential oil, Staphylococcus epidermidis.
Pengaruh Ekstrak Metanol Daun Kayu Kuning (Arcangelisia flava (L.) Merr.) terhadap Kadar Kolesterol Total dan Trigliserida Tikus Hiperlipidemia (The Influence of Methanol Extract of Yellow Root (Arcangelisia flava (L.) Merr.) Leaves on Total Cholesterol Putri Eka Maryani; Evi Umayah Ulfa; Ema Rachmawati
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Hyperlipidemia is heterogeneous disorder that characterized by elevated levels of total cholesterol, low density lipoprotein (LDL), very low density lipoprotein (VLDL), triglyceride, and decreased levels of high density lipoprotein (HDL). The aim of this study was to determine the ability of methanol extract of leaves of Arcangelisia flava to decrease total cholesterol and triglyceride in some variations of dosage. Five groups experimental animal (each consisting of 4 rats) which have been induced by high fat and fructose diet for 45 days, followed by oral treatment of extract at a dose of 250 mg/kg BW, 500 mg/kg BW, 750 mg/kg BW, simvastatin 0.36 mg/200 g BW (control (+)), and CMC Na 1% (control (-)) for 7 days. The blood was taken on day 8, then was measured the total cholesterol and triglyceride. The test results showed that extract could decrease total cholesterol and triglyceride in line with increasing doses. According to ANOVA, there are significant differences between treatment groups (p
Pengaruh Ekstrak Metanol Daun Kayu Kuning (Arcangelisia flava (L.) Merr) terhadap Histopatologi Aorta Tikus Wistar Hiperlipidemia (The Influence of Methanol Extract of Yellow Root (Arcangelisia flava (L.) Merr) Leaves on Aortic Histopathology in Hyperli Yuniar Wahyu Rahmawati; Evi Umayah Ulfa; Ema Rachmawati
Pustaka Kesehatan Vol 4 No 2 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Atherosclerosis is the leading cause of heart attack and stroke. Hyperlipidemia is one of the triggers of atherosclerosis through oxidative stress and formation of foam cells. Atherogenic index (the ratio of LDL/HDL) is a predictive parameter of coronary heart disease. A.flava is potential as antiaterosklerosis due to the content of berberine and flavonoids. This study was undertaken to determine the ability of methanolic extract of A.flava leaves (EMDAf) in lowering atherogenic index and the number of foam cells. Five groups experimental animals (each consisting of 4 rats) was induced with high fat and fructose diet for 45 days, followed by administration of EMDAf dose of 250 mg/kg BW, 500 mg/kg BW, 750 mg/kg BW, simvastatin 1.8 mg/kg BW (control (+)), and CMC Na 1% (control (-)) for 7 days treatments. Then the rat arcus aorta was taken for histopathological examination with Masson Trichrome staining. ANOVA test results showed that EMDAf could decrease the atherogenic index, but not significantly (p> 0.05). While the Kruskall Wallis test results showed that the increase dose of EMDAf might improve histopathology of the aorta through a reduction in the number of foam cells significantly compared to the negative control (p <0.05). Berberine contents in EMDAf is 0.046% measured by TLC-densitometry. It could be concluded that methanolic extract of A.flava leaves had the ability to decrease in the atherogenic index value and the number of foam cells.   Keywords: Arcangelisia flava leaves, foam cells, atherogenic index, aorta, histopathology
Uji Aktivitas Antiplatelet Ekstrak Etanol Kubis Merah (Brassica oleracea var. capitata L.) Antiplatelets activity of red cabbage ethanolic extract (Brassica oleracea var. capitata L.) Rizki Rica Rachim Fadilah Putri; Evi Umayah Ulfa; Rini Riyanti
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Antiplatelet aggregation effect of red cabbage ethanolic extract (Brassica oleracea var. capitata L.) was measured by bleeding time on Balb-C mice. The test substances given orally during the period of 8 days. Bleeding time was measured on first day and 9th day. Red cabbage ethanolic extract were divided into 3 doses: wich are 9.69 mg/kg body weight; 19,38 mg/kg body weight, and 38,76 mg/kg body weight. After getting the percentage of bleeding time increase, data were analyzed by analysis of variance test (Anova), followed by individual comparison within the groups by LSD test. The test substances above respectively increased bleeding time 58 ± 16%; 70,7 ± 8,9 %, and 113 ± 12%. As the conclusion, red cabbage ethanolic extract doses 38,76 mg/kg body weight could increase bleeding time as well as positive controle 129 ± 8,2%. Keywords: antiplatelets, Brassica oleracea red cabbage, bleeding time.
Uji Aktivitas Anti Hiperurisemia Ekstrak n-Heksana, Etil Asetat, dan Etanol 70% Biji Jinten Hitam (Nigella sativa) terhadap Mencit Hiperurisemia (Anti Hyperuricemic activity of n-Hexane, Ethyl acetate, and Ethanol 70% of Black Cumin Seed (Nigella sativa) Umar Dian Prambudi Kusuma; Siti Muslichah; Evi Umayah Ulfa
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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The aim of this study was to determine the in vivo anti-hyperuricemic effect of black cumin extract. Black cumin was extracted with n-hexane, ethyl acetate, and ethanol 70% continously. Fifteen mice were divided into five groups K-, K+, P1, P2, and P3. Group K- as negative control (chicken liver juice 0,2 % b/v) and group K+ as positive control (allopurinol 10 mg/kg b.w.). Group P1, P2, and P3 received n-hexane, ethyl acetate, and ethanol 70% black cumin extract. The extracts were given to mice in 200 mg/kg b.w dose for 4 days. The blood samples were taken an hour after potassium oxonate injection (300 mg/kg b.w) to inhibit uricase enzym. The data was collected and analyzed using one way ANOVA and LSD test. The result showed that activity of ethyl acetate extract of black cumin has no significant difference of possitive control. Based on phytochemistry screening it has terpenoid, saponin, and alkaoid. Terpenoid has anti-hyperuricemic effect but its mechanism still unknown.   Keywords: hyperuricemic, Nigella sativa, terpenoid.
Pengaruh Gel Binahong (Anredera cordifolia (Ten.) Steenis) terhadap Penyembuhan Luka Tikus Diabetes yang Diinduksi Aloksan (The Effect of Binahong (Anredera cordifolia (Ten.)Steenis) Gel on Wound Healing Process of Diabetic Rats Induced by Alloxan) Shinta Cholifatu Rohma; Evi Umayah Ulfa; Diana Holidah
Pustaka Kesehatan Vol 3 No 3 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Diabetes Mellitus (DM) is a chronic disease that can be indicated by the increase of blood glucose level. Diabetic ulcer is one of complication caused by DM. Binahong (Anredera cordifolia (Ten). Steenis) has a potential effect to heal the wound. The purpose of this study was to examined the effect of binahong gel on the wound healing process of diabetic rats induced by alloxan. The observation was done on the wound's histopathological preparation. The wound were made on diabetic rats, then the binahong gel at a dose of 100 mg and 200 mg were spread on to their wounds. The observation of qualitative descriptive parameters consisted of epithelial tissue, collagen, and new blood vessels formation. The results showed that binahong gel was able to accelerate the wound healing process of diabetic ulcer.   Keywords: Anredera cordifolia, diabetic ulcer, wound healing, alloxan, histopathological profile
Uji Aktivitas Antihiperurisemia Ekstrak n-Heksana, Etil Asetat, dan Etanol 70% Daun Tempuyung (Sonchus arvensis L.) pada Mencit Jantan Hiperurisemia Ferani Cendrianti; Siti Muslichah; Evi Umayah Ulfa
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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The purpose of this research was to study the activity of Sonchus arvensis leaves extract as antihyperuricemics agent on hyperuricemic male mice. Eighteen male mice were divided into six groups, i.e the first group as a normal control K(N); the second group as a negative control K(-) treated chicken liver juice 0,2 % b/v orally; the third group as a positive control K(+) treated allopurinol 10 mg/kg BW orally; the fourth to six groups P1, P2, and P3 were treated with nhexane, ethyl acetate, and ethanol 70% of Sonchus arvensis leaves extract 300 mg/kg BW orally for 4 days. Pottasium oxonate 300 mg/kg BW i.p was induced 1 hour before the blood samples was collected. The blood samples was taken from vena ophtalmicus. The concentration of uric acid was determined with FS DHBSA. Data was analyzed using one way ANOVA and LSD test. The result showed that activity of ethyl acetate and ethanol 70% extract of Sonchus arvensis leaves has significant effect in reducing the concentration of uric acid in hyperuricemic mice. Base on phytochemistry screening it has flavonoid and terpenoid. The substance that estimated has antihyperuricemic activity was flavonoid.