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All Journal e-Journal Pustaka Kesehatan Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Pharmaceutical Science and Technology Pharmacon Pharmascience JSFK (Jurnal Sains Farmasi & Klinis) JPSCR : Journal of Pharmaceutical Science and Clinical Research PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Ilmu Kefarmasian Indonesia Abdimasku : Jurnal Pengabdian Masyarakat Jurnal farmasi UIN Alauddin Makassar Nusantara Science and Technology Proceedings
Bawon Triatmoko
Fakultas Farmasi Universitas Jember
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Pengaruh Metode Ekstraksi terhadap Kadar Fenol dan Flavonoid Total, Aktivitas Antioksidan serta Antilipase Daun Jati Belanda (Guazuma ulmifolia) Nuri Nuri; Endah Puspitasari; Mochammad Amrun Hidayat; Indah Yulia Ningsih; Bawon Triatmoko; Dewi Dianasari
Jurnal Sains Farmasi & Klinis Vol 7, No 2 (2020): J Sains Farm Klin 7(2), Agustus 2020
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (917.154 KB) | DOI: 10.25077/jsfk.7.2.143-150.2020

Abstract

The leaf of Guazuma ulmifolia has been used traditionally for antiobesity. The activity of antiobesity was affected by the content of bioactive compounds. Extraction is the primary step to obtain bioactive compounds from plant material. The method and solvent used for extraction are crucial factors to produce extracts that have a high amount of active compounds. This study aims to determine the total phenolic and total flavonoids content from ethanolic extracts, water extract, and infusions of G. ulmifolia leaf and to evaluate the antioxidant and antilipase activity. Folin-Ciocalteu method was used to determine the phenolic content, while flavonoid content determination was done using aluminium chloride colorimetric assay. The antioxidant activity was done using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and the antilipase activity was quantified using p-nitrophenol release from p-nitrophenyl butyrate (p-NPB) substrate-colorimetric assay. The result of G. ulmifolia leaf extraction showed that the highest yield was obtained from water extraction (10.50%). Whereas, the ethanolic extract was showed the highest total phenolic content (67.761±1.811 mg GAE/g extract) and the highest total flavonoid content (124.643 ± 1.033 mg QE/g extract). The same extract also exhibited the highest antioxidant activity (IC50 = 6.544 ± 0.271 µg/mL) and antilipase activity (IC50 = 307.280 ± 21.430 µg/mL).
Molecular Docking Analysis of Anti-malarial Compounds as Plasmepsin IV Inhibitor from Targeted Indonesian Medicinal Plants Arthur Hariyanto Prakoso; Muhammad Habiburrohman; Wilda Nur Rohmatillah; Bawon Triatmoko; Ari Satia Nugraha
Nusantara Science and Technology Proceedings International Conference on Life Sciences and Biotechnology (ICOLIB)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0801

Abstract

Malaria is one of the major causes of death in tropical and sub-tropical countries, caused by the infection of the protozoan parasite (Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium knowlesi). As the prevalence of parasite drug-resistant strains increasing, alternative medicine to eliminate malaria is needed. In this study, a molecular docking protocol was employed to predict and select natural compounds from Indonesian medicinal plants as an antimalarial drug candidate. The docking protocol was validated by the RMSD value of crystal versus docking calculation. From 43 species of plants, 238 total compounds were collected and docked into Plasmepsin IV (PMIV) enzyme which plays a role in the nutrition uptake of Plasmodium in human blood circulation. This enzyme was collected from a protein database with codename 5I70. The protocol was produced an acceptable RMSD value of 1.435 ?. The docking experiment resulted in AM202 (Cassiamin B) from Cassia siamea as the best potent Plasmepsin IV inhibitor. This compound has the potential candidate for future anti-malarial drugs. Cassiamin B had an affinity value of -11.2 kcal/mol which was higher than PMIV’s native ligand (-3.8 kcal/mol).
Ethnopharmacology and Computer-Aided Tandem Protocol to Search for Antimalarial Agents from Indonesian Medicinal Plants: HAP Inhibitor Adinda Kusuma Pertiwi; Muhammad Habiburrohman; Yoshinta Debby; Bawon Triatmoko; Ari Satia Nugraha
Nusantara Science and Technology Proceedings International Conference on Life Sciences and Biotechnology (ICOLIB)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0802

Abstract

The anti-malarial potency of Indonesian medicinal plants was evaluated through computational study. From 43 Indonesian medicinal plants, 238 previously reported compounds were carefully docked into HAP (histo-aspartic protease) with codename 3FNT in which the enzyme plays an important catalytic role in Plasmodium falciparum innate metabolism. Exhaustive docking experiments produced 6 best hits molecules including AM210 (4-hydroxy- 3-methoxy strychnine), AM213 (protostrychnine), and AM216 (pseudostrychnine) which have less free energy compared to HAP native ligand, 1,2-ethanediol. This study revealed the potency of Strychnos nux-vomica L. as a source for antimalaria and support its traditional claims.
The Virtual Screening to Search Proplasmepsin II Inhibitor from Indonesian Medicinal Plant Phytochemicals: Anti-Malaria Muhammad Habiburrohman; Wilda Nur Rohmatilah; Arthur Hariyanto Prakoso; Bawon Triatmoko; Ari Satia Nugraha
Nusantara Science and Technology Proceedings International Conference on Life Sciences and Biotechnology (ICOLIB)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0804

Abstract

The indigenous people of Indonesia have used medicinal plants to survive infectious diseases, including malaria. The knowledge has been passed through the generation and a limited number of the plants have been studied properly. Malaria infection has been an endemic and complicated problem in the archipelago where drug-resistant cases worsen the situation. Natural product study without pharmacological information has been a drawback to the development of natural-based antimalarial. Computational chemistry protocol gives lower cost facilitation to later a conventional in vitro bioassay. In this study, virtual screening was deployed to find a Proplasmepsin II enzyme inhibitor, in which the enzyme plays an important in this parasite metabolism. The enzyme (1PFZ) was collected from the PDB database followed by docking validation before exhaustive docking calculation of 238 compounds from 43 Indonesian medicinal plants. The docking protocol was valid as indicated by rmsd value of 1.275 Å. One top hit molecule, AM56, was gained with its free energy of binding value of 10.8 kcal/mol which is better than the interaction of the native ligand, propane-1,2,3-Triol, with the free energy of binding ( G) score of 3.9 kcal/mol. AM56 was a secondary metabolite of Borassus flabellifer and its anti-plasmodium was previously studied. However, the mechanism of action of AM56 was never been reported. This study was able to discover a molecular scaffold of proplasmepsin II enzyme inhibitor and can be used as a pathway to QSAR study of AM56 semi-synthetic derivatives.
Virtual Screening the Interaction of Various Compound from Indonesian Plants with the HGXPRT Enzyme to Find a Novel Antimalarial Drug Wilda Nur Rohmatillah; Naura Bathari Winarto; Arthur Hariyanto Prakoso; Bawon Triatmoko; Ari Satia Nugraha
Nusantara Science and Technology Proceedings International Conference on Life Sciences and Biotechnology (ICOLIB)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0805

Abstract

Medicinal plants have been a notable source for antimalarial agents. This study was aimed to investigate the antimalarial potency of Indonesian medicinal plants used traditionally in malarial fever therapy. A total of 238 compounds derived from 43 plants traditionally used to alleviate malarial fever were collected and loaded into molecular docking protocol. The compounds were screened against Hypoxanthine-Guanine-XanthinePhosphoribosyltransferase (HGXPRT, 3OZF) using the AutoDock Vina software 1.1.2. The compound is important for the purine synthesis of the parasite. The experiment resulted in AM125 (20-isoveratramine) from Cyanthillium patulum to possess the highest affinity with free energy (?G)-11 kcal/mol, which is better than HGXPRT native ligands (-6.4kcal/mol). This suggested Cyanthillium patulum was a potential source for antimalarial agents in which its constituents, 20-isoveratramine might responsible for the claims.
In Silico Study of Histo-aspartic Protease (HAP) Inhibitor from Indonesian Medicinal Plants: Anti-malarial Discovery Dinar Mutia Rani; Muhammad Habiburrohman; Yoshinta Debby; Bawon Triatmoko; Ari Satia Nugraha
Nusantara Science and Technology Proceedings International Conference on Life Sciences and Biotechnology (ICOLIB)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0803

Abstract

Malaria is an infectious disease caused by Plasmodium sp with the highest clinical incidence of 12.07% in Indonesia. New anti-malaria compounds are needed to replace antimalarial drugs that are already resistant nowadays. One of the efforts to find a new anti-malaria drug is through research on traditional medicinal plants used by Indonesian tribes from the ethnopharmacology database. In silico studies provide saving solutions in the process of computer-aided drug design. Histo-aspartic protease (HAP) is essential for the growth of Plasmodium falciparum and has been validated as an antimalarial drug target. Therefore, molecular docking was used to provide new insights into the development of drugs by targeting HAP protease. There are 238 compounds from 43 medicinal plants used as targeting ligand in this study prepared by Autodock Vina for an automated docking tool. The comprehensive docking protocol was valid showed by the RMSD value of 1,275 Å. The result obtained that AM50 (borrasosides A) from Borassus flabellifer was found to have the least affinity score of -10.1 kcal/mol higher compared to the native ligand. In conclusion, we are assuming that the mechanism of borrasosides A compound might get involved with HAP. Further protocols are required to prove the HAP inhibition towards Plasmodium falciparum.
Isolasi Jamur Tanah dari Muara Sungai di Desa Kilensari Kecamatan Panarukan Serta Skrining Aktivitas Antibakteri Terhadap Pseudomonas aeruginosa Amrina Rosyada Fajriyanti; Bawon Triatmoko; Ari Satia Nugraha
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 6, No 2 (2021)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v6i2.44007

Abstract

Kasus infeksi oleh bakteri menjadi sepuluh besar kasus yang mengakibatkan kematian di Indonesia dengan persentase 9,5 %. Penggunaan antibiotik yang tidak tepat untuk mengatasi masalah infeksi dapat menyebabkan resistensi antibakteri. Untuk mengatasi hal tersebut, banyak dilakukan pengembangan terhadap agen-agen antibakteri. Banyak penelitian menunjukkan bahwa aktivitas antibakteri dapat ditemukan pada mikroorganisme dalam tanah. Penelitian ini dilakukan penelusuran dan isolasi fungi tanah muara di Desa Kilensari Kecamatan Panarukan serta skrining aktivitas antibakteri terhadap Pseudomonas aeruginosayang bertujuan untuk memperoleh kandidat antibakteri yang potensial.Metode yang digunakan dalam penentuan aktivitas antibakteri pada penelitian ini yaitu metode mikrodilusi berdasarkan protokol Clinical and Laboratory Standards Institute tahun 2015. Hasil yang diperoleh dari tahap isolasi yaitu 6 isolat fungi tanah yang diberi kode IS-PN-A1, IS-PN-A2, IS-PN-T1, IS-PN-T2, IS-PN-B1 dan IS-PN-B2. Isolat yang didapatkan dilakukan skrining awal uji aktivitas antibakteri dengan metode ujikontak langsung dengan bakteri Pseudomonas aeruginosa. Zona bening yang terbentuk menunjukkan aktivitas antibakteri dari isolat. Isolat fungi tanah muara kemudian dilakukan proses fermentasi untuk memproduksi metabolit sekunder. Hasil fermentasi di lakukan proses ekstraksi menggunakan etil asetat yang nantinya ekstrak akan digunakan dalam uji aktivitas antibakteri menggunakan metode mikrodilusi. Hasil uji aktivitas antibakteri menunjukkan bahwa fungi tanah muara sungai Desa Kilensari Kecamatan Panarukan memiliki aktivitas antibakteri terhadap bakteri Pseudomonas aeruginosa. Persen penghambatan fungi tanah muara dengan nama isolat IS-PN-A1 menghasilkan persen penghambatan sebesar 74,8±2,4%; IS-PN-A2 33,2±7,0%; IS-PN-T1 70,2±4,8%; IS-PN-T2 53,1±3,6%; IS-PN-B1 29,7±4,0%; IS-PN-B2 45,8±2,1 %. 
Skrining Fitokimia dan Uji Aktivitas Antibakteri Ekstrak dan Fraksi Tanaman Senggugu (Rotheca serrata (L.) Steane & Mabb.) terhadap Pseudomonas aeruginosa Ifan Arif Maulana; Bawon Triatmoko; Ari Satia Nugraha
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 5, No 1 (2020)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v5i1.32200

Abstract

Irasionalitas terapi antibiotik yang tidak terkontrol dilaporkan menyebabkan bakteri resisten antibiotik, sehingga memicu penemuan agen antibakteri baru. Eksplorasi keanekaragaman tanaman obat Indonesia masih menjanjikan sebagai agen antibakteri yang potensial, termasuk tanaman senggugu (Rotheca serrata (L.) Steane & Mabb.). Penelitian ini bertujuan mengetahui aktivitas antibakteri dan kandungan fitokimia dari ekstrak dan fraksi senggugu terhadap bakteri Pseudomonas aeruginosa ATCC 27853. Ekstraksi dilakukan dengan menggunakan metanol. Fraksinasi bertingkat dilakukan untuk mendapatkan fraksi n-heksana, diklorometana, etil asetat, dan residu. Skrining fitokimia dilakukan untuk menentukan alkaloid, terpenoid/steroid, flavonoid, dan polifenol menggunakan metode KLT. Uji aktivitas antibakteri dilakukan menggunakan metode mikrodilusi untuk menentukan nilai IC50. Ekstrak mengandung terpenoid/steroid, flavonoid, polifenol. Fraksi n-heksana dan diklorometana mengandung terpenoid/steroid. Fraksi etil asetat dan residu mengandung flavonoid dan polifenol. Nilai IC50 terendah dicapai oleh fraksi n-heksana sebesar 176,919 ± 6,303 µg/mL. Ekstrak dan fraksi senggugu memiliki aktivitas antibakteri yang moderat.
Penelusuran dan Isolasi Fungi Tanah Muara Pelabuhan Besuki Serta Skrining Aktivitas Antibakteri Terhadap Pseudomonas aeruginosa ATTCC 27853 Mariatul Kibthiyyah; Bawon Triatmoko; Ari Satia Nugraha
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 6, No 2 (2021)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v6i2.44275

Abstract

Penyakit infeksi merupakan salah satu penyebab utama kematian di dunia. Terapi infeksi bakteri dengan antibiotik yang tidak tepat penggunaannya dapat menyebabkan resistensi. Penemuan antibiotik baru dapat berasal dari bahan alam, salah satunya fungi. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etil asetat hasil fermentasi fungi khamir tanah muara terhadap Pseudomonas aeruginosa.Isolasi fungi tanah dilakukan menggunakan media Potato dextrose Agar (PDA) dilarutkan dalam air laut. Proses tersebut menghasilkan 7 isolat fungi kemudian disebut IS-PB-A1, IS-PB-A2, IS-PB-A3, IS-PB-T1, IS-PB-T2, IS-PB-B1, IS-PB-B2. Semua isolat fungi memiliki potensi dalam menghambat pertumbuhan bakteri dengan uji antagonis. Pengujian aktivitas antibakteri  dilanjutkan menggunakan metode mikrodilusi. Aktivitas antibakteri disajikan dalam bentuk persen penghambatan. Hasil uji antibakteri dari tujuh ekstrak konsentrasi 100 µg/mL menunjukkan persen penghambatan tertinggi dari kode IS-PB-A2 (84,7 ± 1,4%) dan terendah dari kode IS-PB-T2 (30,5 ± 3,3 %). Senyawa yang berperan menghambat pertumbuhan bakteri dalam penelitian ini belum bisa dipastikan sehingga diperlukan penelitian lebih lanjut.
Skrining Fitokimia dan Uji Aktivitas Antibakteri Ekstrak dan Fraksi Daun Senggugu (Rotheca serrata (L.) Steane & Mabb.) terhadap Staphylococcus aureus Nimas Ayu Amanda Putri; Bawon Triatmoko; Ari Satia Nugraha
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 18 No. 01 Juli 2021
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pharmacy.v18i1.4809

Abstract

Indonesia merupakan salah satu negara berkembang yang masih memiliki banyak permasalahan di bidang kesehatan, salah satu diantaranya adalah penyakit infeksi. Pencarian atau penelusuran agen antibakteri baru perlu dilakukan untuk mendapatkan alternatif antibiotik yang memiliki aktivitas terhadap mikroorganisme patogen. Salah satu cara untuk mendapatkan antibiotik baru adalah dengan memanfaatkan agen antibakteri yang bersumber dari tanaman seperti senggugu (Rotheca serrata (L.) Steane & Mabb.). Penelitian ini dilakukan untuk mengetahui kandungan golongan senyawa kimia dan aktivitas anibakteri yang ada pada daun senggugu. Daun senggugu diekstraksi dengan menggunakan metanol. Ekstrak dari daun senggugu kemudian difraksinasi dengan metode fraksinasi bertingkat menggunakan pelarut heksana, diklorometana dan etil asetat. Skrining fitokimia dilakukan dengan menggunakan metode KLT (Kromatografi Lapis Tipis) untuk melihat secara kualitatif adanya kandungan alkaloid, terpenoid, polifenol dan flavonoid. Uji aktivitas antibakteri dilakukan dengan metode mikrodilusi untuk mendapatkan nilai IC50 ekstrak dan fraksi daun senggugu terhadap Staphylococcus aureus ATCC 25923. Uji aktivitas antibakteri ekstrak dan fraksi daun senggugu menunjukkan bahwa fraksi heksana (323,729±2,025 µg/mL) memiliki aktivitas yang paling besar dengan kandungan senyawa kimia berupa terpenoid.
Co-Authors Achmad Syarifudin Noor Adinda Kusuma Pertiwi Afif Rifqie Maulana Afrian Rosyadi Agustinus Yuswanto Amrina Rosyada Fajriyanti Ari Satia Nugraha Arthur Hariyanto Prakoso Arthur Hariyanto Prakoso Arthur Hariyanto Prakoso Asia Asia Dewi Dianasari Dewi Dianasari Dianasari, Dewi Dinar Mutia Rani Dita Isnaini Prabawati Dwi Nurahmanto Dwi Nurahmanto Endah Puspitasari Fauzia Ken Nasititi Fawwas Batio Putra Pamungkas Gani Asri Muharam Huda Almuttaqin Ifan Arif Maulana Indah Yulia Ningsih Ita Husnul Chotimah Mariatul Kibthiyyah Marsalita Irine Prabandari Mochammad Amrun Hidayat Muhammad Habiburrohman Muhammad Habiburrohman Muhammad Habiburrohman Muhammad Zainul Arifin Nafa Rosyida Zanuba Nasititi, Fauzia Ken Naura Bathari Winarto Nimas Ayu Amanda Putri Nuri Nuri Nuri Nuri Nuri Nuri Saeful Akhmad Tauladani Savitri, Ganevi Resta Triana Hertiani Wilda Nur Rohmatilah Wilda Nur Rohmatillah Wilda Nur Rohmatillah Yoshinta Debby Ziyan Nihlatul Millah