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All Journal Jurnal Bios Logos BERKALA SAINSTEK Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) BIOEDUKASI Jurnal Biologi Tropis JPBIO (Jurnal Pendidikan Biologi) Bioscientist : Jurnal Ilmiah Biologi InSTEM Life Science and Biotechnology Bioeksperimen Jurnal Bioteknologi & Biosains Indonesia (JBBI) Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Lelono, Asmoro
Jurusan Biologi FMIPA Universitas Jember, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Salmonella typhimurium injection as an immunostimulant: Study on chickens (Gallus gallus domesticus) lelono, Asmoro; Surya, Rizky
Life Science and Biotechnology Vol. 1 No. 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.43625

Abstract

Salmonella is a gram-negative bacteria commonly found in poultry-based products such as eggs and meat, which can affect quality and human health. The presence of these bacteria in poultry management can be traced to feed, water, substrate, and interactions with the environment. This study aims to investigate at the immune system of the avian at the age when the immune system begins to be produced independently through injection of S.typhimurium colonies. Salmonella culture at a density of 10-8 and 10-12 is injected into the digestive tract, and the increase in body temperature is measured immediately after injection. Two weeks later, an analysis of leukocyte differentiation is carried out. We found that salmonella injection affected body temperature and the rate of increase in body mass, both based on treatment and sex. This indicates that the immune system has worked even though the leukocyte differentiation indicators have not shown significant results. Chicks at the age of one month are able to show an immune response to bacterial infections through self-defence mechanisms. Further research needs to be carried out to understand whether leukocyte differentiation stimulates leukocyte variation with age.
Does the chick of domestik chicken (Gallus gallus domesticus) in early development would able to witstand the injection of crude LPS? lelono, Asmoro; Laensugi, Agatha Dei Gratia; Arimurti, Sattya
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53503

Abstract

Preventing bacterial infections in poultry farms is a key aspect of effective disease management strategies. Enhancing innate immunity serves as a vital preventive measure in chicken farming. Lipopolysaccharide (LPS), a fundamental component of the bacterial cell wall, has been successfully utilized to boost the innate immune response in susceptible poultry hosts. This study focused on examining the impact of administering crude LPS from Salmonella typhimurium to a domestic chicken (Gallus gallus domesticus) strain during its early developmental stages up to the pre-maturation phase. Immune responses in the chicks were evaluated by observing changes in body temperature and leukocyte differentiation. The chickens were divided into two groups: control (injected with 0.85% NaCl) and treatment (injected with the LPS). The injections were applied in three different ages 7 days, 24 days, and 41 days. The body temperature and leukocyte differential data were collected following the LPS injection. Results showed a significant difference in body temperature and heterophil at the ages of 24 and 41, indicating an immune response characterized by inflammation and elevated heterophil levels. There was a significant increase in basal temperature during early development in line with chicks' development to maintain their homeostatic system. This study concludes that the chicks demonstrated resilience to the presence of LPS by exhibiting heightened responses. The increasing of heterophils concentration provided strong evidence of their immune capability to overcome bacterial infections
The Negative effects on the immune system of native chicken embryos (Gallus gallus domesticus) due to extreme environmental fluctuations before incubation lelono, Asmoro
Life Science and Biotechnology Vol. 3 No. 1 (2025): May 2025
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Climate variability can influence avian reproductive success by altering environmental factors critical to egg viability and embryonic development. In avian species such as Gallus gallus domesticus, the period before incubation exposes eggs to fluctuating conditions that may affect their survival and the offspring’s physiological performance. This study evaluated the effects of pre-incubation environmental conditions on the growth and immune response of native chickens. Fertilized eggs were subjected to different temperature and humidity treatments before incubation to simulate natural ecological variations. The findings revealed that neither low-temperature exposure nor excessive moisture significantly reduced egg viability or embryonic development. Post-hatching observations, including biometric parameters and tonic immobility tests, showed no significant behavioral differences among treatment groups. Although all chicks exhibited daily weight gain, those from the control group displayed more stable growth patterns, indicated by lower standard error values. Measurements of innate immune response, inferred from body temperature fluctuation, were comparable across groups. These results suggest that native chicken eggs possess physiological plasticity that enables them to maintain normal embryogenesis and immune development under variable environmental conditions
Moringa oleifera gum effect on blood sugar levels and rat pancreas histology induced by Streptozotocin Utami, Eva Tyas; Fajariyah, Susantin; Lelono, Asmoro; Nihayah, Husnatun
JPBIO (Jurnal Pendidikan Biologi) Vol 9, No 1 (2024): April 2024
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v9i1.3184

Abstract

Moringa gum is a product that people can use as an anti-hyperglycemic agent. This study aimed to determine the effectiveness of Moringa gum in reducing blood sugar levels and improving the histology structure of the rat pancreas induced by streptozotocin (STZ). This study used 21 male rats divided into 3 groups including control, diabetes (STZ), and diabetes (STZ + 3% Moringa gum). STZ treatment at a single dose (45 mg/kg BW) was given intraperitoneally, while Moringa gum was given through drinking water for 2 weeks. Blood sugar levels were measured on days 0, 7th, 14th, 21st, and 28th. The results showed that  STZ administration induced diabetes in rats with blood sugar levels of 487 mg/dL on the 14th day.  Moringa gum administration was able to reduce blood sugar levels on days 21st and 28th respectively to 306 and 234 mg/dl. The histology structure of the pancreas of rats treated with gum also showed improvement. This study concludes that Moringa gum is effective in lowering blood sugar levels in rats even though it still does not show normal blood sugar levels, and can repair STZ-induced damage to the histology structure of the rat pancreas.
IN-SILICO ANALYSIS OF THE INTERACTION BETWEEN D7 PROTEIN FROM THE SALIVARY GLAND OF Ae. albopictus AND Thromboxane A2 FOR DEVELOPING ANTIPLATELET AGENT Wathon, Syubbanul; Senjarini, Kartika; Oktarianti, Rike; Lelono, Asmoro
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.8176

Abstract

The salivary glands of mosquito vector diseases contain various biological components which facilitate blood-feeding into the host's body. These components are mostly protein molecules. Numerous protein molecules in the salivary glands have gained substantial research emphasis to determine their role and function, including those in the salivary glands of Ae. albopictus. D7 protein is the main component in Aedes salivary glands, which aids in inhibiting platelet aggregation by binding to the Thromboxane A2 (TxA2) during the blood-feeding. TxA2 is a eicosanoid molecule that stimulates platelet aggregation. The protein's ability to bind TxA2 shows that this protein has potential as a new antiplatelet agent. The examination of the D7 protein in binding TxA2 was performed through an in-silico approach using the molecular docking method. This research included selecting the 3D model of the D7 protein and the TxA2 ligand, preparing the 3D model of the D7 protein, native ligands, and test ligands, targeted molecular docking method, validating the molecular docking, analysis and visualization of the docking results. The molecular docking validation shows an RMSD value of 1.657 Å. The results of molecular docking show an ΔG value of -5.60 kcal/mol, meaning that the D7 protein can bind to the TxA2 ligand stably and spontaneously. The active site of the D7 protein in binding the TxA2 ligand consists of several amino acid residues, namely THR 190, GLU 268, TYR 178, PHE 154, ILE 175, ARG176, VAL 293, TYR 248, and TYR 178. The ability of D7 protein to bind TxA2 as an inducer of platelet aggregation has demonstrated its potential as a novel antiplatelet agent. These results can pave further development of drug discovery in the medical and pharmaceutical fields.
PURIFIKASI FRAKSI PROTEIN IMUNOGENIK 47 kDa DARI KELENJAR SALIVA Aedes albopictus SEBAGAI TARGET PENGEMBANGAN VAKSIN DENGUE BERBASIS VEKTOR Wathon, Syubbanul; Rahmawati, Itsna; Oktarianti, Rike; Lelono, Asmoro; Senjarini, Kartika
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.810

Abstract

Kapasitas vektorial Ae. albopictus telah diketahui sebagai vektor potensial virus dengue yang mengakibatkan DBD. Transmisi virus dengue terjadi ketika Ae. albopictus melakukan blood feeding ke manusia yang difasilitasi oleh aktivitas biologis protein pada kelenjar saliva vektor. Penelitian kami sebelumnya menunjukkan fraksi protein 47 kDa dari kelenjar saliva Ae. albopictus bersifat imunogenik. Protein 47 kDa merupakan serpins family yang diduga sebagai antikoagulan dan berperan sebagai protease inhibitor sehingga mempermudah transmisi virus dengue. Peran protein tersebut mengindikasikan adanya potensi dalam pengembangan vaksin penghambat transmisi patogen melalui vaksinasi melawan protein tersebut sehingga dapat menghambat transmisi dengue. Studi potensi protein tersebut memerlukan ekstrak murni, maka purifikasi protein target merupakan langkah yang sangat penting. Penelitian ini dimulai dengan landing collection dan rearing Ae. albopictus, isolasi dan ekstraksi protein kelenjar saliva, isolasi fraksi protein 47 kDa melalui SDS-PAGE, purifikasi protein, analisis dot blot dan western blot. Analisis SDS-PAGE menunjukkan pita tunggal protein target 47 kDa dari kelenjar saliva Ae. albopictus yang berhasil dipurifikasi menggunakan Electroeluter. Analisis dot blot menunjukkan protein 47 kDa terdeteksi bersifat imunogenik dan dikonfirmasi melalui analisis western blot bahwa protein target hasil purifikasi memiliki berat molekul 47 kDa.
Mosquitoes' larval habitat characteristics and Aedes aegypti resistance status to malathion in Jember Nihayah, Husnatun; Purwatiningsih, Purwatiningsih; Utami, Eva Tyas; Fajariyah, Susantin; Lelono, Asmoro; Puspitasari, Reni; Rohmah, Hidayatur
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1037

Abstract

Water-holding containers serve as breeding sites for Aedes aegypti. The control of mosquito breeding sites and the use of household insecticides are some of the DHF control methods. The use of household insecticides over a long period can reduce the susceptibility of mosquitoes to these insecticides. This research aims to determine the mosquito's habitat characteristics and to determine the Aedes aegypti resistance status to malathion in Jember.  A total of 61 houses were surveyed, and the various types of containers encountered at the sampling locations were recorded. Larvae and pupae found at the sampling location are brought and hatched in the laboratory. Aedes aegypti was tested for resistance status to malathion insecticide using a CDC bottle bioassay and biochemical nonspecific esterase enzyme activity. A total of 140 containers were found in this study, and 36.43% of them are Aedes aegypti larvae/pupae habitat. Types of containers used as mosquito larval habitat are bottles/glasses, bathtubs, used basins/buckets, water storage buckets, trash cans, refrigerator water containers, used cans, gutters, livestock drinking water containers, fish ponds, plant pots, used livestock cages, used toilets, used jugs, used animal feeders, and used aquariums. The mortality of Aedes aegypti reached 42.3% (resistant). Meanwhile, the results of biochemical tests showed an increase in the activity of the nonspecific esterase enzyme. This study concludes that Aedes aegypti from Jember has been resistant to malathion insecticide
Struktur Histologi Trabekula Femur Mencit (Mus musculus L) Orkidektomi Pasca Pemberian Ekstrak tempe Kedelai Susantin Fajariyah; Siti Nafi’atul Muta’alimah; Eva Tyas Utami; Asmoro Lelono; Husnatun Nihayah
JURNAL BIOS LOGOS Vol. 14 No. 3 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i3.56668

Abstract

Testosterone deficiency is caused by, among other things, old age, chronic disease, and testicular cancer, which results in decreased bone density. Estrogen plays a more important role in the process of bone growth and resorption than the hormone testosterone. Therefore, to prevent a decrease in density in conditions of testosterone deficiency, it is necessary to administer external phytoestrogens, including tempeh, containing isoflavones deidzein dan genistein. The aim of this study was to determine the effect of administering tempeh extract on the histological structure of the femoral trabeculae of orchidectomized mice. Mice were divided into 4 groups, namely negative control (without orchidectomy), positive control (orchidectomy), and 2 orchidectomy treatment groups, followed by administration of tempe extract at a dose of 0.6 g/ml/day (D1) and 1.2 g/ml/day (D2). Tempeh extract was administered by gavage for 15 days after a 30-day healing period for bilateral orchidectomy. One day after the last administration of tempeh extract, the mice were anesthetized and dissected to remove the femur bones to make preparations using the paraffin method and HE staining. The parameters observed were the number of osteoblasts and osteoclasts and the thickness of the femoral trabeculae. Giving soybean tempeh extract at a dose of 0.6 mg/ml/day by gavage for 15 days can cause an increase in the number of osteoblasts and trabecular thickness, as well as reducing the number of osteoclasts in the femur of orchidectomized mice.
Preliminary study of acute febrile response to crude LPS extract from Salmonella typhimurium in domestic chicken (Gallus gallus domesticus) AKY strain Wijaya, Rizky Surya; Lelono, Asmoro; Oktarianti, Rike
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.16139

Abstract

Salmonella is a genus that commonly attacks the health of poultry, especially chickens. One type of poultry that is widely attacked by S. typhimurium is chickens. S. typhimurium infects the host with Lipopolysaccharides (LPS). This study aims to determine the innate immune response of domestic chickens to LPS-based vaccines S. typhimurium as the first step in vaccine development. The sample was in the form of female domestic chickens of the AKY strain which was divided into 3 groups, the control group was injected using 0.1ml of PBS (Phosphate Buffer Saline) treatment group 1 was injected with LPS with an adjuvant with a concentration of 0.5mg/kg as much as 0.1ml. Treatment group 2 was injected with LPS with an adjuvant with a concentration of 1mg/kg of 0.1ml. Injection treatment group using Incomplete Freund Adjuvant (IFA). The results obtained were that the treatment groups had an increase in temperature at the 1st hour to the highest in the 3rd hour with a temperature range of 42.5°C to 42.6°C, contrast to the control group which has a static temperature with a range of 41°C to 41.8°C. The comparison of chicken body temperature among treatment groups using a Liner Mixed Model revealed a significant difference (p < 0.001) between the treatment groups and the control group. These results indicate that subcutaneous LPS administration induces a controlled acute febrile response, which is consistent with involvement of innate inflammatory pathways.
DHF Prevention Efforts in Sumbersari Jember: Assessment of Aedes aegypti Resistance Status to Deltamethrin Reni Puspitasari; Hidayatur Rohmah; Husnatun Nihayah; Purwatiningsih; Eva Tyas Utami; Susantin Fajariyah; Asmoro Lelono
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19883

Abstract

This study aims to determine the resistance status of Ae. aegypti to the deltametrin insecticide in Sumbersari Jember Village. A total of 100 2nd generation female Ae. aegypti were tested using the CDC bottle bioassay with a deltamethrin dose of 10 μg/ml and a diagnostic time of 30 minutes. Molecular detection using AS-PCR was also performed to detect kdr mutations at position 1016 of the VGSC gene. Mortality data was calculated using Ms.Excel with a confidence level of 95%. The molecular wight of the PCR product is calculated using a gel analyzer application.  The results of the CDC bottle bioassay show that the Ae aegypti population is still tolerant to the insecticide deltamethrin (91,25% in mortality rate). However, molecular analysis confirms the presence of a mutation at position 1016 of the voltage-gated sodium channel (VGSC) gene (homozygous mutation), which is known to be the primary target of pyrethroid insecticides. These findings indicate that although phenotypic resistance has not yet fully developed, the mosquito population has shown early indications of target-site insensitivity to deltamethrin, which has the potential to develop into resistance under sustained selection pressure. Further use of deltamethrin insecticide may potentially increase phenotypic resistance, namely a decrease in mosquito mortality after exposure to deltamethrin insecticide. Therefore, an integrated vector control strategy and routine monitoring of mosquito resistance status in the region are required.
Co-Authors Achmad Sjaifullah Adelia Nur Fahmi Adi Sucipto, Adi Akhmad Rikhwan Alfiyani, Nur Asyia Arif Mohammad Siddiq Arofa, Resmining Mega Arumiannisa Altamaputri Aurellia, Naurah Septa Dewi, Rosita Fitrah Dwi Nur Rikhma Sari Eva Tyas Utami Farahdillah Rafiatul Nurhasanah Hari Sulistyowati Hidayat Teguh Wiyono Hidayatur Rohmah Intani, Rini Nur Kartika Senjarini Kholiq, Nur Laensugi, Agatha Dei Gratia Nailatur Rifdah Nihayah, Husnatun Nur Aisyah Septiana Nur Asyia Alfiyani Nurul Fajril Azizah Purwatiningsih Purwatiningsih Purwatiningsih Purwatiningsih Puspitasari, Reni Putra, Iguh Widigda Rahmawati, Itsna Reni Puspitasari Rike Oktarianti Rini Nur Intani Rizky Surya Wijaya Rohmah, Hidayatur Sattya Arimurti Siti Nafi’atul Muta’alimah Surya, Rizky Susantin Fajariyah Tabah Wibisono, Hariyo Wathon, Syubbanul Wijaya, Rizky Surya Wimbaningrum, Retno Yudha Nurdian