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All Journal Jurnal Penelitian Saintek Jurnal Biologi Udayana AL KAUNIYAH Jurnal Sains Dasar AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Scientiae Educatia: Jurnal Pendidikan Sains JURNAL BIOLOGI INDONESIA Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA AL-HAYAT: Journal of Biology and Applied Biology Jurnal Mangifera Edu Bioscientist : Jurnal Ilmiah Biologi Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Kingdom (The Journal of Biological Studies) IJOBI Biosaintifika: Journal of Biology & Biology Education
Paramita Cahyaningrum Kuswandi
Jurusan Pendidikan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Neuroscience Physics Public Health Social Sciences

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Journal : Kingdom (The Journal of Biological Studies)

 1 
THE EFFECT OF SEVERAL CONCENTRATIONS OF GLYCINE AMINO ACID ADDITIONS IN MS MEDIUM TOWARS CALLUS GROWTH OF KONJAC (Amorphophallus muelleri) BY in vitro CULTURE Rizka Anisa Rennytasari; Paramita Cahyaningrum Kuswandi
Kingdom (The Journal of Biological Studies) Vol 8, No 2 (2022): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v8i2.18153

Abstract

Konjac (Amorphophallus muelleri) is a plant that can be a source of carbohydrates and has high economic value. To accelerate the fulfillment of porang seeds in order to support maximum economic potential, it is necessary to develop alternative propagation techniques,which is in vitro culture. This study aimed to determine the effect of adding the glycine amino acid to MS medium on the growth of konjac callus and to determine the best concentration for callus growth in konjac plant tissue culture. This research was using frog bulb (bulbil) for the explant. In this study used a completely randomized design (CRD) with glycine amino acid concentrations from 0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L, 4 mg/L, dan 5 mg/L with 3 replications. Data were analyzed using SPSS software with one way ANAVA and further test using DMRT 5%.The results showed that the addition of the glycine amino acid to MS medium affected the callus growth of konjac plant tissue culture based on the analysis of variance (ANAVA) test. The best concentration used based on the Duncan Multiple Range Test (DMRT) 5%, was 4 mg/L with the average callus appearance time of 3.06 wac and the average bending time of 2.1 wac.
PENGARUH PENAMBAHAN BAP TERHADAP INDUKSI KALUS TANAMAN PORANG SECARA IN VITRO Lailani, Zhafira Istiqla; Kuswandi, Paramita Cahyaningrum
Kingdom: The Journal of Biological Studies Vol 9, No 1 (2023): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v9i1.18481

Abstract

Abstrak. Porang merupakan sumber karbohidrat yang bernilai ekonomi tinggi. Untuk memenuhi permintaan porang sebagai produk diversifikasi pangan maupun produk ekspor, perlu dukungan ketersediaan benih dan budidaya yang memadai. Perbanyakan secara in vitro menjadi alternatif dalam pemenuhan bibit porang yaitu melalui metode kultur kalus dengan menggunakan zat pengatur tumbuh BAP pada media MS. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan BAP terhadap induksi kalus tanaman porang (Amorphophallus muelleri). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan beberapa konsentrasi BAP yang terdiri dari 6 level (0, 1, 2, 3, 4, 5) mg/liter sebanyak 6 ulangan. Data yang diperoleh dianalisis dengan uji one way ANOVA dan uji lanjut menggunakan DMRT 5%. Hasil penelitian menunjukkan bahwa penambahan BAP pada media MS berpengaruh pada pertumbuhan kalus kultur jaringan tanaman porang berdasarkan uji one way ANOVA. Konsentrasi 2 mg/L BAP merupakan konsentrasi yang paling cepat dalam menginduksi pertumbuhan kalus dengan rata-rata waktu pelengkungan kalus 1,450 mst dan rata-rata waktu munculnya kalus 2,633 mst.Kata Kunci: induksi kalus; BAP; Amorphophallus muelleri.
Co-Authors ,, Wirdateti ,, Wirdateti Agung Wijaya Subiantoro Aji, Ibnu Kholdun Sukma Anazifa, Rizqa Devi Anazifa, Rizqa Devi Anggi Tias Pratama Arief Priyadi Arsyada*, Tasya Asih, Ni Putu Sri Ayuningtyas, Dwi Evy Yulianti Fajar Prasetya isnaeni, bekti Ixora Sartika Mercuriani Kartika Ratna Pertiwi Khan, Farwa Hafeez Khoirun Nisa Lailani, Zhafira Istiqla Lili Sugiyarto Lili Sugiyarto Lili Sugiyarto Lili Sugiyarto Md Hoirul Azri Ponari Mohd Syafik Mohamad Hamdan Mohd Syahmi Salleh Mualimin Mualimin Muhamad Fahmi Yunus Paidi Paidi Prasetya, Fajar Pratiwi, Risha Amilia Pratomo, Ibnu Rizka Anisa Rennytasari Rizka Apriyani Putri Sahnaz, Shafila Selma Nafilatul Husna Suratsih Suratsih Tien Aminatun Tutiek Rahayu Wiharyanti, Risma Wiharyanti, Risma Wulandari*, Novia Dian Kartika Wulandari, Sri Wijayanti Wulandari, Sri Wijayanti Zain, Haniza Hanim Mohd