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All Journal Lontar Komputer: Jurnal Ilmiah Teknologi Informasi BIOTROPIA - The Southeast Asian Journal of Tropical Biology Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Jurnal Teknosains Jurnal Terapan Abdimas Fountain of Informatics Journal Khadimul Ummah Journal of Social Dedication Agroindustrial Technology Journal Jurnal Agroteknologi Merdeka Pasuruan
Trisnaningrum, Niken
Program Studi Agroteknologi, Fakultas Sains Dan Teknologi, Universitas Darussalam Gontor, Ponorogo, Jawa Timur, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Public Health Social Sciences Veterinary Other

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ISOLATION AND CLONING OF cDNA OF GENE ENCODING FOR METALLOTHIONEIN TYPE 2 FROM MELASTOMA AFFINE SUHARSONO, SUHARSONO; TRISNANINGRUM, NIKEN; SULISTYANINGSIH, LULUT DWI; WIDYASTUTI, UTUT
BIOTROPIA Vol. 16 No. 1 (2009): BIOTROPIA Vol. 16 No. 1 June 2009
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.362 KB) | DOI: 10.11598/btb.2009.16.1.64

Abstract

Metallothionein is an important protein for detoxifying heavy metal ions. This research was conducted to isolate and clone cDNA of gene encoding for metallothionein type 2 from Melastoma affine. Total RNA was isolated from young leaves. Total cDNA was synthesized from the total RNA by reverse transcription. The MaMt2 cDNA was successfully isolated by PCR technique. The MaMt2 cDNA was inserted into pGEM-T Easy and the recombinant plasmid was successfully introduced into Escherichia coli DH5α. DNA sequencing analysis showed that this cDNA is full length consisting of 246 pb encoding 81 amino acid residues. This cDNA is identical to mRNA of AtMt2 from Arabidopsis thaliana. It does not contain any restriction sites found in the cloning sites of pGEM-T easy. The deduced protein of MaMT2 contains 14 cysteine residues distributed in the Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys motifs.   Key words: cDNA, metallothionein, Melastoma affine, cloning, cysteine
Co-Authors Agung Toto Wibowo Alfu Laila Ashari, Riza Ema Rachmawaty Hamawi, Mahmudah Herlangga, Muhammad Ilham Mufandi Imam Fauzi Annur Juliana, Dara Puspita Panca Jumhurul Umami Lulut Dwi Sulistyaningsih Lutfy Ditya Cahyanti, Lutfy Ditya Moch. Nasheh Annafii Muhammad Hanif Muhammad Muhammad Niken Sylvia Puspitasari Oddy Virgantara Putra Parwi, Parwi Suharsono Suharsono Triana Harmini Umi Isnatin Use Etica UTUT WIDYASTUTI Wawa, Laola Badu