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UJI AKTIVITAS ANTIBAKTERI FRAKSI DAN SENYAWA AKTIF DAUN BENALU JERUK NIPIS (DENDROPHTOE PETANDRA (L.)MIQ.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI Masayu Farah Diba; Arwan bin Laeto; Septi Purnamasari; Rara Inggarsih
JURNAL KEDOKTERAN DAN KESEHATAN Vol 8, No 2 (2021)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/V8I2.13128

Indonesia menjadi salah satu negara yang memanfaatkan tanaman sebagai obat alternatif untuk menangani penyakit yang diderita masyarakat. Daun benalu jeruk nipis banyak mengandung senyawa aktif yang dapat menjadi antibakteri. Tujuan penelitian adalah menguji aktivitas antibakteri fraksi dan senyawa aktif daun benalu jeruk nipis (Dendrophtoe petandra (L.)Miq.) terhadap bakteri Staphylococcus aureus dan Escherichia coli. Penelitian ini menggunakan sel bakteri Staphylococcus aureus dan Escherichia coli sebagai objek dan menggunakan beberapa konsentrasi dari fraksi n-heksan benalu jeruk nipis. Penelitian menerapkan metode eksperimental laboratorium secara in vitro yang bersifat eksploratif analitik melalui teknik fraksinasi cair-cair (FCC) dengan pelarut n-heksan, etil asetat dan metanol yang diuapkan menggunakan Rotary evaporator dan teknik pengeringan menggunakan hair dryer. Skrining fitokimia bertahap dilakukan untuk mengidentifikasi flavonoid, alkaloid, saponin, tanin, terpenoid, steroid, dan triterpenoid yang ditandai adanya busa dan terbentuknya cincin berwarna. Hasil menunjukkan bahwa berat fraksi yang diperoleh menggunakan pelarut n-Heksan = 1,32 gram (3,38%), etil asetat = 32,76 gram (83,94%), dan etanol air = 4,95 gram (12,68%). Hasil uji skrining fitokimia diperoleh pada ekstrak (4 senyawa kimia), fraksi n-heksan (2 senyawa), fraksi etil asetat (4 senyawa) dan fraksi etanol air (2 senyawa kimia). Hasil uji aktivitas antibakteri diperoleh diameter zona hambat fraksi n-Heksan pada E.coli (9,25±1,15) dan S.aureus (9,41±0,52); etil asetat pada E.coli (10,67±1,15) dan S.aureus (10,33±0,52). Hasil uji konsentrasi hambat minimum fraksi n-Heksan 80.000 ug/ml pada E.coli (12,33±0,95) dan S.aureus (12,75±0,52), dan 625 ug/ml pada E.coli (7,29±0,18) dan S.aureus (0,00±0,00).Kata kunci: antibakteri, senyawa aktif, daun benalu jeruk nipis, Staphylococcus aureus, Escherichia coli

Gendola Leaf Ethyl Acetate Fraction (Basella rubra Linn) Reduces Spermatozoa Motility and Viability in Vitro Lusia Hayati; Joko Marwoto; Septi Purnamasari; Yuni Fitriayanti
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 3 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i3.213

A B S T R A C TBackground. The use of plants as anti-fertility in men has been done to reduce sideeffects. The Gendola plant is one of the Basellaceae family plants, which is a naturalmedicinal plant in Indonesia. Gendola leaves contain phenol compounds, flavonoids,tannins, steroids and triterpenoids which can have cytotoxic activity. This studyaims to determine the in vitro anti sperm activity of the ethyl acetate fraction ofGendola leaves on male spermatozoa. Methods. This study is an experimentallaboratory in vitro study using male sperm preparations. The sample used was 20men for each group, both the group was given the gondola leaf fraction and thecontrol group. The treatment group was given ethyl acetate fraction of Gendola leaves(Basella rubra Linn.) With a concentration of 0.05% for 5, 10 and 15 minutes, theviability and motility of sperm were calculated. Data analysis was performed usingthe Mann Whitney test (p <0.05) using SPSS release for Windows software. Results.The results showed that the ethyl acetate fraction affected the motility and viabilityof spermatozoa. When compared with the control group, the motility treatment groupobtained was classified as non-progressive. In the treatment group, the viability ofspermatozoa was significantly different at minute 5, minute 10 and minuted 15 (p<0.05). Conclusion. Ethyl acetate fraction has potential as an anti sperm inreducing sperm motility and viability.

Dermatoglyphy in Breast Cancer Patients: A Systematic Review Rara Inggarsih; Akhyar Dyni Zakyah; Lusia Hayati; Joko Marwoto; Septi Purnamasari; Arwan Bin Laeto; Masayu Farah Diba
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 11 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i11.368

Background. Breast cancer is one of the four types of cancer among women and is the most frequently diagnosed in most countries. Breast cancer occurs due to DNA damage and genetic mutations affected by exposure to estrogen, inheritance of damaged DNA, or pro-cancer genes such as BRCA1 and BRCA2. Therefore, a family history of ovarian cancer or breast cancer increases the risk of developing breast cancer. The embryo of the breast develops around the age of 6 weeks of pregnancy. Similar to breast development, fingerprint patterns also develop during the 6-13 weeks of pregnancy. Thus, the genetic message contained in the genome occurred during that period and was reflected in the dermatoglyphic pattern.Methods. The literature search was systematically used using PubMed, Cochran, Google scholar, and other Gray literature between 2010-2020. Of the 69 publications identified, 21 met the criteria and were included in the review. The review is carried out following the provisions of PRISMA (Preferred Reporting Items for Systematic Review).Results. This systematic review showed fairly consistent findings in breast cancer patients who tended to have more whorl fingerprint patterns and larger ATD angles. For radial loops, ulnar loops and arches were minor compared to the control group potential as an initial screening tool in at-risk groups.Conclusion. Long-term and follow-up studies with larger sample sizes in various ethnicities are needed to validate dermatoglyphics in anthropometric measurements as a promising marker of breast cancer.

Risk Factors of Vesicovaginalis Fistule in Obstetric and Gynecologic Division of Dr. Mohammad Hoesin General Hospital in 2018-2020 Nabila Istighfarin; Hadrians Kesuma Putra; Eka Handayani Oktharina; Ratih Krisna; Septi Purnamasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 3 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i3.476

Background. A vesicovaginal fistula (FVV) is a direct pathological connection between the bladder and vagina resulting in uncontrolled leakage of urine into the vagina from the bladder. Based on the cause, fistulas are divided into two, namely obstetric fistula and gynecological fistula. The risk factors that influence the occurrence of FFV consist of obstetric risk factors (age, parity, delivery method, duration of delivery, birth weight) and gynecological risk factors (history of gynecological surgery, history of pelvic radiotherapy, history of gynecologic malignancy). The purpose of this study was to determine the relationship between risk factors and the incidence of vesicovaginal fistula. Methods. This research is an observational-analytic study with a cross-sectional method. The number of samples obtained was 74 patients who met the inclusion criteria using secondary data from medical records. Analysis of the data used is the chi-square test. Results. There were 37 people who experienced vesicovaginal fistula. Where there were 9 patients with obstetric vesicovaginal fistula (24.3%) and 28 people (75.7%). From the Chi-Square test analysis, there was a significant relationship between delivery method (p=0.029), birth weight (p=0.029), history of gynecological surgery (p=0.038), history of pelvic radiotherapy (p=0.016), history of gynecological malignancy (p=0.016). =0.010). Meanwhile, there was no significant relationship between age (p=0,347), parity (p=1,000), and duration of labor (p=0,082). Conclusion. There was a significant relationship between the delivery method, birth weight, history of gynecological surgery, history of pelvic radiotherapy, history of gynecological malignancy. There was no significant relationship between age, parity, and duration of vaginal delivery.

Analisis Profil Eritrosit Tikus Putih (Rattus norvegicus) Pasca Diet Vegetarian Arwan Bin Laeto; Rara Inggarsih; Septi Purnamasari; Masayu Farah Diba; Fahmil Ikhsan Taharu
Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton Vol 8 No 1 (2022): Sang Pencerah: Jurnal Ilmiah Universitas Muhammadiyah Buton
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Muhammadiyah Buton

Saat ini telah banyak penelitian yang menggunakan tikus putih (Rattus norvegicus), namun informasi terkait profil eritrosit pada hewan coba tersebut masih sangat sedikit, khususnya pada tikus putih yang diberi diet vegetarian. Penelitian ini bertujuan untuk menganalisis profil eritrosit tikus putih pasca diet vegetarian. Metode penelitian ini menggunakan pendekatan eksploratif analitik melalui ekperimental laboratorium invivo dengan desain posttest-only design with nonequivalent group. Total sampel tikus putih adalah 28 ekor yang dibagi menjadi 4 kelompok dan diberikan diet selama 6 minggu. Sampel darah tikus putih diambil melalui area sinus orbital dan dilakukan analisis jumlah sel darah merah, kadar hemoglobin, persentase hematokrit, indeks MCV, MCH dan MCHC. Hasil menunjukkan jumlah sel darah putih, kadar indeks MCV, MCH dan MCHC tertinggi pada kelompok tikus putih diet vegetarian tipe quasi, yaitu masing-masing 7,58 x 106 sel/µL, 53,7 fL, 18,6 pg/sel dan 34,5 g/dL. Kadar hemoglobin dan persentase hematokrit tertinggi pada kelompok tikus putih diet vegetarian tipe lacto-ovo, yaitu masing-masing 15,84 g/dL dan 47,88 %. Nilai-nilai komponen profil eritrosit pada tikus putih dapat disebabkan oleh beberapa faktor, meliputi usia, perilaku aktif, kondisi lingkungan pemeliharaan dan komposisi nutrisi dalam pakan yang diberikan.

Gendola Leaf Ethyl Acetate Fraction (Basella rubra Linn) Reduces Spermatozoa Motility and Viability in Vitro Lusia Hayati; Joko Marwoto; Septi Purnamasari; Yuni Fitriayanti
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 3 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i3.213

A B S T R A C TBackground. The use of plants as anti-fertility in men has been done to reduce sideeffects. The Gendola plant is one of the Basellaceae family plants, which is a naturalmedicinal plant in Indonesia. Gendola leaves contain phenol compounds, flavonoids,tannins, steroids and triterpenoids which can have cytotoxic activity. This studyaims to determine the in vitro anti sperm activity of the ethyl acetate fraction ofGendola leaves on male spermatozoa. Methods. This study is an experimentallaboratory in vitro study using male sperm preparations. The sample used was 20men for each group, both the group was given the gondola leaf fraction and thecontrol group. The treatment group was given ethyl acetate fraction of Gendola leaves(Basella rubra Linn.) With a concentration of 0.05% for 5, 10 and 15 minutes, theviability and motility of sperm were calculated. Data analysis was performed usingthe Mann Whitney test (p <0.05) using SPSS release for Windows software. Results.The results showed that the ethyl acetate fraction affected the motility and viabilityof spermatozoa. When compared with the control group, the motility treatment groupobtained was classified as non-progressive. In the treatment group, the viability ofspermatozoa was significantly different at minute 5, minute 10 and minuted 15 (p<0.05). Conclusion. Ethyl acetate fraction has potential as an anti sperm inreducing sperm motility and viability.

Dermatoglyphy in Breast Cancer Patients: A Systematic Review Rara Inggarsih; Akhyar Dyni Zakyah; Lusia Hayati; Joko Marwoto; Septi Purnamasari; Arwan Bin Laeto; Masayu Farah Diba
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 11 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i11.368

Background. Breast cancer is one of the four types of cancer among women and is the most frequently diagnosed in most countries. Breast cancer occurs due to DNA damage and genetic mutations affected by exposure to estrogen, inheritance of damaged DNA, or pro-cancer genes such as BRCA1 and BRCA2. Therefore, a family history of ovarian cancer or breast cancer increases the risk of developing breast cancer. The embryo of the breast develops around the age of 6 weeks of pregnancy. Similar to breast development, fingerprint patterns also develop during the 6-13 weeks of pregnancy. Thus, the genetic message contained in the genome occurred during that period and was reflected in the dermatoglyphic pattern.Methods. The literature search was systematically used using PubMed, Cochran, Google scholar, and other Gray literature between 2010-2020. Of the 69 publications identified, 21 met the criteria and were included in the review. The review is carried out following the provisions of PRISMA (Preferred Reporting Items for Systematic Review).Results. This systematic review showed fairly consistent findings in breast cancer patients who tended to have more whorl fingerprint patterns and larger ATD angles. For radial loops, ulnar loops and arches were minor compared to the control group potential as an initial screening tool in at-risk groups.Conclusion. Long-term and follow-up studies with larger sample sizes in various ethnicities are needed to validate dermatoglyphics in anthropometric measurements as a promising marker of breast cancer.

Risk Factors of Vesicovaginalis Fistule in Obstetric and Gynecologic Division of Dr. Mohammad Hoesin General Hospital in 2018-2020 Nabila Istighfarin; Hadrians Kesuma Putra; Eka Handayani Oktharina; Ratih Krisna; Septi Purnamasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 3 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i3.476

Background. A vesicovaginal fistula (FVV) is a direct pathological connection between the bladder and vagina resulting in uncontrolled leakage of urine into the vagina from the bladder. Based on the cause, fistulas are divided into two, namely obstetric fistula and gynecological fistula. The risk factors that influence the occurrence of FFV consist of obstetric risk factors (age, parity, delivery method, duration of delivery, birth weight) and gynecological risk factors (history of gynecological surgery, history of pelvic radiotherapy, history of gynecologic malignancy). The purpose of this study was to determine the relationship between risk factors and the incidence of vesicovaginal fistula. Methods. This research is an observational-analytic study with a cross-sectional method. The number of samples obtained was 74 patients who met the inclusion criteria using secondary data from medical records. Analysis of the data used is the chi-square test. Results. There were 37 people who experienced vesicovaginal fistula. Where there were 9 patients with obstetric vesicovaginal fistula (24.3%) and 28 people (75.7%). From the Chi-Square test analysis, there was a significant relationship between delivery method (p=0.029), birth weight (p=0.029), history of gynecological surgery (p=0.038), history of pelvic radiotherapy (p=0.016), history of gynecological malignancy (p=0.016). =0.010). Meanwhile, there was no significant relationship between age (p=0,347), parity (p=1,000), and duration of labor (p=0,082). Conclusion. There was a significant relationship between the delivery method, birth weight, history of gynecological surgery, history of pelvic radiotherapy, history of gynecological malignancy. There was no significant relationship between age, parity, and duration of vaginal delivery.

ACUTE RESPONSE OF LEUCOCYTE TOTAL OF WHITE RAT (RATTUS NORVEGICUS) WITH VARIATIONS VEGETARIAN DIETARY IN STRENUOUS EXERCISE Arwan Bin Laeto; Septi Purnamasari; Rara Inggarsih; Masayu Farah Diba
Majalah Kedokteran Sriwijaya Vol 54, No 1 (2022): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/mks.v54i1.16139

Gaya hidup sehat menjadi hal penting yang perlu diperhatikan dan dijadikan prinsip dalam menjaga derajat kesehatan. Namun, kesibukan harian dalam memenuhi membuat masyarakat kurang memperhatikan diet dan pola aktivitas fisik yang baik dan benar, sehingga berpotensi menimbulkan penyakit yang dapat dinilai salah satunya melalui total leukosit. Penelitian ini bertujuan untuk menganalisis respon akut total leukosit tikus putih (Rattus norvegicus) pada variasi diet vegetarian pada olahraga berat. Metode penelitian berupa eksperimental in vitro melalui pendekatan eksploratif analitik dengan menggunakan desian pre-posttest design with nonequivalent groups. Penelitian ini menggunakan hewan coba tikus putih (Rattus norvegicus) sebagai sampel dengan pemberian tiga tipe diet vegetarian dan diet standar. Pengukuran total leukosit dilakukan sebelum dan sesudah perlakuan olahraga berat berupa berenang hingga lelah. Data setiap kelompok dianalisis secara statistik menggunakan uji-t. Hasil menunjukkan bahwa terdapat perubahan signifikan total leukosit pasca olahraga berat pada kelompok diet vegetarian tipe quasi p=0,028 (p<0,05), lacto-ovo p=0,007 (p<0,05) dan diet standar p=0,045 (p<0,05), sedangkan diet vegetraian tipe vegan tidak menunjukkan perubahan total leukosit yang signifikan pasca olahraga berat p=0,752 (p<0,05). Kesimpulannya, perubahan respon akut total leukosit tikus putih pasca olahraga berat terjadi pada diet vegetarian tipe quasi dan lacto-ovo.

PENERAPAN LATIHAN HINGGA LELAH TERHADAP JUMLAH ERITROSIT DAN HEMOGLOBIN TIKUS PUTIH (Rattus Norvegicus) DENGAN VARIASI DIET VEGETARIAN Arwan Bin Laeto; Rara Inggarsih; Masayu Farah Diba; Septi Purnamasari
Sains Olahraga : Jurnal Ilmiah Ilmu Keolahragaan Vol 6, No 2: Oktober 2022
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/so.v6i2.30139

Kesibukan seharian membuat banyak orang melakukan olahraga secara tidak tepat, yakni hingga mencapai kelelahan, termasuk pada kelompok yang menerapkan diet vegetarian yang saat ini trend dilakukan. Penelitian ini bertujuan untuk menganalisis pengaruh latihan hingga lelah terhadap jumlah eritrosit dan hemoglobin tikus putih (Rattus norvegicus) dengan variasi diet vegetarian. Penelitian ini merupakan ekperimental laboratorium secara in vitro yang bersifat eksploratif analitik dengan desain posttest-only design with nonequivalent groups. Sampel penelitian sebanyak 28 ekor tikus putih yang dikelompokkan menjadi 4 kelompok, yaitu kelompok dengan diet vegetarian tipe quasi, lacto-ovo, vegan dan standar. Jumlah eritrosit dan hemoglobin diukur sebelum dan setelah intervensi latihan hingga lelah. Data dianalisis menggunakan uji-t. Hasil penelitian menunjukkan terdapat pengaruh yang signifikan latihan hingga lelah terhadap jumlah eritrosit pada kelompok diet vegetarian tipe quasi (p=0,028) dan kelompok standar (p=0,045). Selain itu, terdapat pengaruh signifikan latihan hingga lelah terhadap kadar hemoglobin pada kelompok diet vegetarian tipe lacto-ovo (p=0,041) dan vegan (p=0,038). Kesimpulan bahwa latihan hingga lelah mempengaruhi jumlah eritrosit tikus putih dengan diet tipe quasi dan standar, serta mempengaruhi kadar hemoglobin dengan diet vegetarian tipe lacto-ovo dan vegan.Kata kunci: eritrosit, hemoglobin, latihan hingga lelah, vegetarian

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