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All Journal Majalah Kedokteran Bandung Mutiara Medika: Jurnal Kedokteran dan Kesehatan Jurnal Kedokteran Anatomica (Anatomica Medical Journal) JURNAL IBNU SINA BIOMEDIKA Hasil Pengabdian Kepada Masyarakat JURNAL ILMIAH MAKSITEK JURNAL ILMIAH SIMANTEK JURNAL ILMIAH KOHESI Ihsan: Jurnal Pengabdian Masyarakat Zona Kedokteran: Program Studi Pendidikan Dokter Universitas Batam Jurnal Pandu Husada JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia
Ance Roslina
Universitas Muhammadiyah Sumatera Utara
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Journal : Mutiara Medika: Jurnal Kedokteran dan Kesehatan

 1 
Effects of AntiMycobacterium Tuberculosis Nanoherbal Legundy Leaf Extract (Vitex trifolia) Dwiyanti, Atika; Mulyani, Siti; Yulhan, Oche Axena; Lubis, Humairah Medina Liza; Roslina, Ance
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 1: January 2021
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i1.6497

Abstract

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Indonesia ranks 2nd largest in the world after India. Resistance to anti-tuberculosis drugs is one of the difficulties in treating tuberculosis. This research aim to determine the effectiveness of legundi (Vitex trifolia) leaf extract nanoherbal in killing Mycobacterium tuberculosis. This study was conducted with an experimental design using M. tuberculosis colonies taken from 25 samples of aspirate of tuberculosis patients with lymph nodes and then given the ethanol extract of legundi leaves (70% and 90%) with the process of making nanoparticle extracts. The evaluation of the observations was in the form of measuring the inhibition zone for bacterial growth which were categorized as weak ( 5mm), moderate (6-10mm), strong (11-20 mm) and very strong ( 21mm). The results of the study from 6 repetitions found that the inhibition zone category was 0% weak, 39% moderate, strong 52.3%, and very strong 8.7%. Comparison of the concentration of legundi leaf extract to the diameter of the bacterial growth inhibitory zone was analyzed using the Kruskal Wallis test, which found a significant relationship with p value = 0.000 ( 0.05). Legundi's leaf extract can be used as an antibacterial agent for M. tuberculosis. 
Effects of AntiMycobacterium Tuberculosis Nanoherbal Legundy Leaf Extract (Vitex trifolia) Atika Dwiyanti; Siti Mulyani; Oche Axena Yulhan; Humairah Medina Liza Lubis; Ance Roslina
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 1 (2021): January
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i1.6497

Abstract

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Indonesia ranks 2nd largest in the world after India. Resistance to anti-tuberculosis drugs is one of the difficulties in treating tuberculosis. This research aim to determine the effectiveness of legundi (Vitex trifolia) leaf extract nanoherbal in killing Mycobacterium tuberculosis. This study was conducted with an experimental design using M. tuberculosis colonies taken from 25 samples of aspirate of tuberculosis patients with lymph nodes and then given the ethanol extract of legundi leaves (70% and 90%) with the process of making nanoparticle extracts. The evaluation of the observations was in the form of measuring the inhibition zone for bacterial growth which were categorized as weak (5mm), moderate (6-10mm), strong (11-20 mm) and very strong ( 21mm). The results of the study from 6 repetitions found that the inhibition zone category was 0% weak, 39% moderate, strong 52.3%, and very strong 8.7%. Comparison of the concentration of legundi leaf extract to the diameter of the bacterial growth inhibitory zone was analyzed using the Kruskal Wallis test, which found a significant relationship with p value = 0.000 (0.05). Legundi's leaf extract can be used as an antibacterial agent for M. tuberculosis. 
Immunocytochemical Study of Rabbit-Polyclonal to Mycobacterium Tuberculosis, AB905: Improving Diagnostic of Tuberculous Lymphadenitis Humairah Medina Liza Lubis; Emni Purwoningsih; Ance Roslina; Muhammad Al Anas
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 2 (2021): July
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i2.11559

Abstract

Tuberculous lymphadenitis (TBLN) is the most common form of extrapulmonary tuberculosis. However, the optimal diagnosis using Fine-Needle Aspiration Cytology (FNAC) or excisional biopsy is uncertain. This research aims to improve the diagnostic of TBLN with FNAC and immunocytochemistry (ICC) compared to the response to antituberculosis therapy. The cross-sectional study involved 43 patients with the criteria for TBLN diagnosis based on the appropriate clinical history of tuberculosis and indicative cytological results. Immunocytochemical examination employed rabbit-polyclonal to Mycobacterium tuberculosis (MTB) antibody (AB905). The MTB expression was found in 35 out of 43 cases (81%) that appropriate cytological features of the tuberculosis process. Meanwhile, eight out of 43 cases (19%) did not express MTB. Diagnostic tests for lesions with a positive cytologic appearance of TBLN and ICC were compared to the response to anti-tuberculosis therapy, revealing the sensitivity, specificity, positive predictive value, and negative predictive value of 95.2%, 75%, 95.2%, and 17%, respectively. Besides, Fisher's exact tests utilized to identify the relationship between two variables; p 0.05 was considered significant. This research found immunocytochemical study was a sensitive and specific tool for improving the diagnostic of TBLN.
Co-Authors Amelia Eka Damayanti Amelia Eka Damayanti Aminuyati ANNISA . ANNISA ANNISA Annisah, Rati Atika Dwiyanti AULIA RAHMI Ayunda Pratiwi L. Tobing Ayunda Pratiwi L. Tobing DANDI NOVTIARDI Dian Erisyawanty Batubara DWINDA RAHMATUN AZHARI PINEM Dwiyanti, Atika FIRSTY DWI HIDAYATI SIRAIT Harahap, Habib Al Rasyid Harahap, Sevani Ayu Humairah Medina Liza Humairah Medina Liza Lubis IHAM HARIAJI Ilham Hariaji Isra Thristy KARINA RAMADHANI Karina Ramadhani Muhammad Al Anas Muhammad Devi Fauzan Rizki Mutiara Yusfah Putri MUTIARA YUSFAH PUTRI S NADHILAH SARI NASUTION NUR HALIZA RASYID Nurfadly, Nurfadly Oche Axena Yulhan Purwoningsih, Emni Putra, Kevin Rhesa Putri, Nabilah Triana RIDHA SAKINAH SOLIN Sarirah, Munauwarus SHAFIRA ROZAANDITA Siti Mulyani TITIN NURJANAH UTARI SEPTIA DHARMA Yenita Yenita Yulhan, Oche Axena