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All Journal Buletin Peternakan Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Matematika Sains dan Teknologi Agrinimal Jurnal Ilmu Ternak dan Tanaman INDRA: Jurnal Pengabdian kepada Masyarakat Pakem : Jurnal Pengabdian Kepada Masyarakat Jurnal Teknologi Lingkungan Lahan Basah Jurnal Kedokteran Hewan Jurnal Nusantara Mengabdi
Rony Marsyal Kunda
Department Of Biology, Faculty Of Mathematics And Natural Science, Universitas Pattimura, Ambon, 97233, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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IDENTIFICATION OF GENETIC DIVERSITY CYTOCHROME OXIDASE SUBUNIT II (COII) MITOCHONDRIAL GENE AS GENETIC MARKER FOR ANISAKIS SPECIES IN Euthynnus affinis Utami, Prasetyarti; Handayani, Niken Satuti Nur; Kunda, Rony Marsyal
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.2960

Abstract

ABSTRACTThis study aimed to get specific genetic marker for Anisakis sp. identification on mackerel tuna using gene sequence cytochrome oxidase subunit II (COII) mitochondrial deoxyribonucleic acid (mtDNA) and to identify taxonomic affiliation between Anisakis sp. from Indonesia and others Anisakis sp. from GenBank database. This study started with sample collections at three fish auctions in Cilacap (Central Java), morphology classification, DNA isolation, and molecular based identification using polymerase chain reaction (PCR) and sequencing methods. Molecular based identification of Anisakis used gene amplification COII mtDNA as a cell target prior to sequence. Morphology characteristic results showed that Anisakis nematodes which infected mackerel tuna classified as type II L3 larvae. Molecular based identification showed significant result, which found 530 bp COII DNA gene fragment similar to target cell. Gene sequencing alignment results of COII Anisakis gene compared with GenBank showed 11 different nucleotide sites that can be used as genetic barcode for Indonesian Anisakis sp. This study showed that Anisakis sp. infected mackerel tuna in Java Sea is Anisakis physeteris and considered as zoonosis.
Pelatihan PELATIHAN PENGGUNAAN ALAT PRAKTIKUM IPA TERINTEGRASI PADA SISWA DAN GURU DI SMA NEGERI 2 SERAM BAGIAN BARAT Kunda, Rony Marsyal; Lokollo, Richard Rudolf; Manuhutu, Fredrik; Salamor, Reinhard; El Anshori, Yuli Tirtariandi
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 4 No 1 (2024): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pakem.4.1.88-93

Abstract

Pembelajaran IPA mestinya lebih menekankan pada dimensi pedagogik modern, yaitu menggunakan scientific approach. Pembelajaran yang relevan diterapkan adalah pembelajaran melalui eksperimen (praktikum). Melalui praktikum siswa memahami fenomena alam yang dikaji dari berbagai sudut sehingga siswa dapat belajar lebih bermakna (meaningful learning). Namun, kendala yang sering dihadapi yaitu kegiatan praktikum memerlukan waktu yang lama jika pembelajarannya dikemas dalam praktikum. Oleh karena itu diperlukan adanya suatu kegiatan pemberdayaan untuk meningkatkan kompetensi guru dalam melaksanakan praktikum. Kegiatan pemberdayaan ini dikemas dalam bentuk inservice training yaitu pelatihan dan pendampingan penggunaan alat praktikum IPA secara terintegrasi pada siswa dan guru di SMA. Pelatihan yang dilaksanakan adalah pelatihan mengembangkan keterampilan dan kreativitas guru IPA dalam menggunakan perangkat praktikum IPA terintegrasi yang memanfaatkan KIT IPA yang sudah diproduksi. Pendampingan juga dilakukan terkait dengan meningkatkan kemampuan guru dalam mengeksplorasi, mengelaborasi dan merefleksi materi ajar IPA. Kegiatan pelatihan dan pendampingan kepada guru dan siswa ini akan dilakukan di SMA Negeri 2 Seram Bagian Barat.
Animals in Tradition and Culture: An Ethnozoological Study of the Indigenous People of Seram Island Marthina Tjoa; Rony Marsyal Kunda; Henderina Lelloltery; Maya MS Puttileihalat
Jurnal Penelitian Pendidikan IPA Vol 11 No 7 (2025): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i7.11286

Abstract

This study aims to examine the relationship between the indigenous people of Seram Island and several species of animals that are utilized for socio-cultural purposes and how the practice of ethnozoology can contribute to sustainable conservation efforts. The research was conducted using an ethnographic approach through in-depth interviews with purposively selected respondents. Qualitative descriptive analysis was conducted systematically based on themes that bind the types of animals studied. The results showed that the practice of ethnozoology includes utilization for ritual purposes related to local community belief values. Some animal species that are utilized for special purposes include Cuscuses (Phalanger spp.) and wild boar (Sus scrofa) which are useful for traditional ceremonies. The animals are killed and the meat is divided to be eaten during the ceremony. Gosong Bird (Eulipoa wallacei) as a Season Marker helps the community in managing agricultural land, marked by the presence or absence of these animals at certain locations. Deer (Rusa timorensis) as a Source of Food and Social Status by utilizing its meat. Cassowary (Casuarius spp.) as a symbol of strength. Hornbill (Rhyticeros plicatus) as a symbol of longevity. These animals are utilized by indigenous people not only for traditional rituals but also for animal protein needs such as cuscuses, Wild Boar and Deer. The needs of the indigenous community for the aforementioned animals have encouraged the community to maintain and preserve them based on their local wisdom such as the application of Sasi.
Polimorfisme Genetik dari Daerah 3'-Non Koding Region (3'-UTR) dari Gen HSP 70 pada Kerbau Moa (Bubalus bubalis) Maman Rumanta; Leonard Raden Hutasoit; Rony Marsyal Kunda; Prasetyarti Utami
Jurnal Penelitian Pendidikan IPA Vol 11 No 9 (2025): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i9.11685

Abstract

The Heat Shock Protein 70 (HSP70) gene, specifically the 3-Untranslated Region (3-UTR), is critical for regulating mRNA stability and translation under heat stress. This study aimed to identify genetic polymorphisms in the 3-UTR of the HSP70 gene in Moa buffalo (Bubalus bubalis) and evaluate its potential role in heat adaptation. A total of 65 hair follicle samples (55 Moa buffalo and 10 Banten buffalo) were collected, followed by amplification by PCR and sequencing of the PCR product of the 3'-UTR regions. Alignment results from the 3'-UTR region showed the presence of 2 major polymorphic SNPs, i.e g.1904C>A, and g.1910A>G. SNP (g.1904C>A) was found to be a cytosine (C) to adenine (A) substitution mutation type, and SNP g.1910A>G an adenine (A) to guanine (G) substitution. The discovery of 2 SNPs in the 3-UTR of the HSP70 gene in Moa buffalo is very important for the development of genetic markers, helping to understand the mechanism of heat adaptation, to the conservation and improvement of livestock performance, especially Moa buffalo living in tropical environments.
Exploration of the Potential of Hotong (Setaria italica) Based on Local Wisdom as a Functional Food for Stunting Prevention in Maluku Jamaludin; Rony Marsyal Kunda; Jilian Risky Huwae; Milda Rianty Lakoan
Jurnal Penelitian Pendidikan IPA Vol 11 No 12 (2025): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i12.13228

Abstract

Stunting remains a major nutritional problem in Indonesia, particularly in vulnerable regions such as South Buru Regency, Maluku Province. Local food diversification represents a strategic approach to improving community nutrition and food security. This study evaluated the nutritional value and socio-ecological potential of Hotong (Setaria italica), a traditional cereal cultivated by local communities in Namrole District and adapted to dryland farming systems. A quantitative descriptive method was applied through a survey of 70 Hotong-farming households in Waenewen Hamlet, Labuang Village, Namrole District, Buru Regency, combined with proximate analysis of Hotong grains based on AOAC standards. The results showed that Hotong contains ash (1.33%), protein (11.60%), fat (3.43%), crude fiber (2.20%), and carbohydrates (65.31%). This nutritional composition indicates that Hotong can contribute up to 40% of protein requirements and approximately 35% of daily iron needs for toddlers aged 1–3 years. Furthermore, 86.67% of farmers reported annual production of ≥ 4 quintals despite relying on traditional cultivation practices, highlighting the crop’s availability and sustainability. These findings demonstrate that Hotong has significant potential as a functional local food for stunting prevention, while also reflecting cultural resilience and local wisdom in maintaining food systems under dryland conditions. Integrating Hotong into regional nutrition and food security programs, including the development of locally processed food products, may contribute to improving the nutritional status of toddlers in dryland areas.
Scanning Electron Microscopy of Goniodes dissimilis Denny, 1842 (Insecta: Phthiraptera) from Domestic Chickens on Seram Island Maria Nindatu; Rony Marsyal Kunda; Mery Pattipeilohy; Yofian Anaktototy; Ventia Cika Tanikwele; Adriana Hakapaa
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.13722

Abstract

Goniodes dissimilis (Denny, 1842) is one of the chewing lice (Ischnocera: Phthiraptera) that commonly infests native chickens (Gallus gallus domesticus). The aims of this study were to describe the morphological and ultrastructural characteristics of G. dissimilis using scanning electron microscopy (SEM). The sampling of Goniodes dissimilis was conducted in Seruawan, Hatusua, and Kamarian villages on Seram Island. SEM preparation was carried out through 2.5% glutaraldehyde fixation, 0.25 M phosphate buffer post-fixation, multistage dehydration, critical-point drying, mounting on stubs, and gold coating before being observed at various magnifications. SEM results showed that G. dissimilis from Seram Island has ultrastructural characteristics, such as the head is circumfasciate with a rounded anterior margin, a complete band on the marginal carina, a pulvinus surrounding the ventral carina, and no hyaline margin. The eyes appear reduced with long ocular setae, while the temporal region has five marginal setae, with setae numbered 2 and 3 presents. The antennae are sexually dimorphic, consisting of a scape, pedicle, and three flagellomeres. In this study, we collected only females of G. dissimilis, and the first flagellomer shows no radial grooves in the sensilla placodea. The thorax shows a pteronotum with several long spines, while the abdomen shows medially separated tergums II–VIII, spiracles on tergums III and VIII, and a thin, membranous sternal plate. The SEM results also show that the female G. dissimilis has terminalia displaying a dorsal plate connected with tergopleurites and a vulva lined with fine setae. This finding represents the first ultrastructural record of the G. dissimilis population on Seram Island, thereby enriching the morphological diversity and distribution data of chewing lice in the Maluku region.
Strategi Konservasi Lingkungan Berbasis Kearifan Lokal: Studi Kasus Desa Kersik Tuo, Kerinci Nova, Lucky Starada; Rumanta, Maman; Kunda, Rony Marsyal
Jurnal Nusantara Mengabdi Vol 5 No 2 (2026): Februari
Publisher : Penerbit Goodwood

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35912/jnm.v5i2.6402

Abstract

Purpose: This study examines the role of local wisdom in supporting environmental conservation in Kersik Tuo Village, Kayu Aro District, Kerinci Regency, Jambi Province. Research Methodology: A mixed-methods approach was employed, combining qualitative data collection through interviews and participatory observation with quantitative analysis using SWOT and the Analytical Hierarchy Process (AHP). Results: Local wisdom practices, including hunting prohibitions, crop rotation, and communal land management (gotong royong), remain actively practiced in Kersik Tuo Village. However, these practices face pressures from modernization, environmental degradation caused by intensive agriculture, and declining awareness among younger generations. SWOT and AHP analyses identified three priority conservation strategies: strengthening customary institutions, integrating local knowledge with modern science, and enhancing community participation. Conclusions: Local wisdom has significant potential to serve as a foundation for sustainable and adaptive environmental conservation, particularly in responding to contemporary ecological challenges. Limitations: This study is geographically limited to Kersik Tuo Village, which may restrict the generalizability of the findings to other regions with different socio-cultural conditions. Contributions: This study contributes to the development of community-based conservation frameworks by demonstrating the strategic importance of indigenous knowledge systems as a complement to scientific conservation approaches.
Impact of Environmental Geographic toward Point Mutations in Exon 1 of Growth Differentiation Factor (GDF9) Gene in Kosta and Lakor Goat Breeds Maman Rumanta; Rony Marsyal Kunda; Slamet Diah Volkandari; Ivan Mambaul Munir
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.3794

Abstract

This study aims to analyze the impact of environmental biogeography on point mutations in Kosta and Lakor goat breeds as local Indonesian germplasm using the GDF9 Exon 1 gene sequence. A total 43 folicle samples of Kosta and Lakor goats which collected in Serang regency and Lakor island were analyzed. To isolate the total genome of the samples, the tissue isolation method was used. The polymerase chain reaction (PCR) method was used for amplification of the exon-1 region of the GDF9 gene with two pairs of primers. Analysis of genetic variation and single nucleotide polymorphisms (SNP) using MEGA software version X. Multiple alignment results showed that there are 2 nucleotides that have mutations, but not encode different amino acids. This results indicates that the mutation has association with litter size of two breeds. This finding shows that Lakor goats have polymorphism i.e SNP g.54C>T which is significantly associated with litter size. The analysis of GDF9 gene showed that all samples from the Two local Indonesian goat breeds (Kosta and Lakor) are  monomorphic. But, although both are monomorphic, geographical isolation still contributes to genetic drift within and between populations.
Biosensors Perancangan dan Pengembangan Biosensor untuk Deteksi Kualitas Perairan Laut Berbasis Piranti Mikrokontroler ESP32: Perancangan dan Pengembangan Biosensor untuk Deteksi Kualitas Perairan Laut Berbasis Piranti Mikrokontroler ESP32 Maman Rumanta; Rony Marsyal Kunda; Fredrik Manuhutu
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.6944

Abstract

Water quality in cultivating marine organisms is a very important part to pay attention to. Prevention of pollution in marine waters needs to be done by recognizing changes in physical, chemical and biological parameters in these waters from normal conditions. To find out these changes, it is necessary to carry out measurements which include parameters that determine the quality of a liquid and carry them out periodically. The measurement parameters measured include pH, Dissolved Oxygen (DO), Electrical Conductivity (EC) and temperature. The use of technological developments, especially in the world of microcontrollers, namely the ESP32 device, makes it possible to design measurement instruments in determining the quality of sea waters to be more efficient and comprehensive. The instrument created in this research will be equipped with several sensors that will read the parameters that determine water quality. The sensors used are pH sensors, DO sensors, EC sensors and temperature sensors. This research aims to create an integrated instrument (pH, DO, ED and Temperature) with instrument performance specifications that have good accuracy and a portable design so that it is easy to use in the field. The research results show thatdevelopment of a biosensor instrument for measuring seawater quality based on the ESP32 DevKit V1 can work well and can display temperature, degree of acidity (pH), DO and EC parameters on the LCD screen according to actual conditions with a difference range in percent between 0.2% - 4.16 %. The accuracy level of the designed tool is classified as having a high accuracy above 95%
Bioaccumulation of Pb in the Gills of Perna viridis (Bivalvia: Mytilidae) in Jakarta Bay, Indonesia and its Histological Alteration Rony Marsyal Kunda; Maman Rumanta; Leonard Raden Hutasoit
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.7109

Abstract

Pollution in Jakarta Bay associated with terrestrial run-off and eutrophication. Bivalves have been recognized as aquatic organisms that play the most dominant role in aquatic ecosystems. Pb is an element that in many cases causes health problems in the liver, kidneys, hematopoietic system and nervous system. This study aims to analyze the bioaccumulation of Pb in the gills of P. viridis in Jakarta Bay, and its histological changes. Bioaccumulation analysis using the AAS and histology changes using transmission electron microscopy (TEM) methods. The results of AAS, showed that the value of Pb concentration in gill tissue at the two seasons did not show a noticeable difference. Pb concentrations in gills were lower at 1, 4, and 5 than at 2 and 3 stations. The results of ultrastructure analysis as an impact of Pb accumulation showed that thickening of the nucleus membrane, and a decrease in the number of microvilli. The thickening of the nucleus membrane, and the decrease in the number of microvilli are cellular responses to the entry of the Pb heavy metal into the cell. Besides, the results of this study showed inflammation, a decrease in the number of mitochondria, as well as causing cristae to be empty.
Co-Authors Adriana Hakapaa Fredrik Manuhutu Fredrik Manuhutu, Fredrik Habibi Habibi Hendrina Lelloltery Hery Wijayanto Heryanus Jesajas Ivan Mambaul Munir JAMALUDIN JAMALUDIN Jamhari Jamhari Jilian Risky Huwae Lakoan, Milda Rianty Leonard R. Hutasoit Maman Rumanta Maman Rumanta Maria Nindatu Marleny Leasa Marthina Tjoa Maya MS Puttileihalat Mery Pattipeilohy Milda Rianty Lakoan Moniharapon, Mechiavel Niken Satuti Nur Handayani Niken Satuti Nur Handayani Nova, Lucky Starada Pieter Kakisina Porumau, Yandri Yanes Prasetyarti Utami Prasetyarti Utami Prasetyarti Utami Richard R Lokollo, Richard R Rini Widayanti Salamor, Reinhard Sandra Sukmaning Aji Selanno, Frenly M. Slamet Diah Volkandari Slamet Diah Volkandari Sri Kurniati Handayani Ucu Rahayu Utami, Prasetyarti Ventia Cika Tanikwele Woro Danur Wendo Yofian Anaktototy Yuli Tirtariandi El Anshori