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All Journal Buletin Peternakan Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Matematika Sains dan Teknologi INDRA: Jurnal Pengabdian kepada Masyarakat Jurnal Sylva Scienteae Pakem : Jurnal Pengabdian Kepada Masyarakat Jurnal Teknologi Lingkungan Lahan Basah Jurnal Kedokteran Hewan
Rony Marsyal Kunda
Department Of Biology, Faculty Of Mathematics And Natural Science, Universitas Pattimura, Ambon, 97233, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Pelatihan PELATIHAN PENGGUNAAN ALAT PRAKTIKUM IPA TERINTEGRASI PADA SISWA DAN GURU DI SMA NEGERI 2 SERAM BAGIAN BARAT Kunda, Rony Marsyal; Lokollo, Richard Rudolf; Manuhutu, Fredrik; Salamor, Reinhard; El Anshori, Yuli Tirtariandi
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 4 No 1 (2024): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pakem.4.1.88-93

Abstract

Pembelajaran IPA mestinya lebih menekankan pada dimensi pedagogik modern, yaitu menggunakan scientific approach. Pembelajaran yang relevan diterapkan adalah pembelajaran melalui eksperimen (praktikum). Melalui praktikum siswa memahami fenomena alam yang dikaji dari berbagai sudut sehingga siswa dapat belajar lebih bermakna (meaningful learning). Namun, kendala yang sering dihadapi yaitu kegiatan praktikum memerlukan waktu yang lama jika pembelajarannya dikemas dalam praktikum. Oleh karena itu diperlukan adanya suatu kegiatan pemberdayaan untuk meningkatkan kompetensi guru dalam melaksanakan praktikum. Kegiatan pemberdayaan ini dikemas dalam bentuk inservice training yaitu pelatihan dan pendampingan penggunaan alat praktikum IPA secara terintegrasi pada siswa dan guru di SMA. Pelatihan yang dilaksanakan adalah pelatihan mengembangkan keterampilan dan kreativitas guru IPA dalam menggunakan perangkat praktikum IPA terintegrasi yang memanfaatkan KIT IPA yang sudah diproduksi. Pendampingan juga dilakukan terkait dengan meningkatkan kemampuan guru dalam mengeksplorasi, mengelaborasi dan merefleksi materi ajar IPA. Kegiatan pelatihan dan pendampingan kepada guru dan siswa ini akan dilakukan di SMA Negeri 2 Seram Bagian Barat.
Animals in Tradition and Culture: An Ethnozoological Study of the Indigenous People of Seram Island Marthina Tjoa; Rony Marsyal Kunda; Henderina Lelloltery; Maya MS Puttileihalat
Jurnal Penelitian Pendidikan IPA Vol 11 No 7 (2025): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i7.11286

Abstract

This study aims to examine the relationship between the indigenous people of Seram Island and several species of animals that are utilized for socio-cultural purposes and how the practice of ethnozoology can contribute to sustainable conservation efforts. The research was conducted using an ethnographic approach through in-depth interviews with purposively selected respondents. Qualitative descriptive analysis was conducted systematically based on themes that bind the types of animals studied. The results showed that the practice of ethnozoology includes utilization for ritual purposes related to local community belief values. Some animal species that are utilized for special purposes include Cuscuses (Phalanger spp.) and wild boar (Sus scrofa) which are useful for traditional ceremonies. The animals are killed and the meat is divided to be eaten during the ceremony. Gosong Bird (Eulipoa wallacei) as a Season Marker helps the community in managing agricultural land, marked by the presence or absence of these animals at certain locations. Deer (Rusa timorensis) as a Source of Food and Social Status by utilizing its meat. Cassowary (Casuarius spp.) as a symbol of strength. Hornbill (Rhyticeros plicatus) as a symbol of longevity. These animals are utilized by indigenous people not only for traditional rituals but also for animal protein needs such as cuscuses, Wild Boar and Deer. The needs of the indigenous community for the aforementioned animals have encouraged the community to maintain and preserve them based on their local wisdom such as the application of Sasi.
Polimorfisme Genetik dari Daerah 3'-Non Koding Region (3'-UTR) dari Gen HSP 70 pada Kerbau Moa (Bubalus bubalis) Maman Rumanta; Leonard Raden Hutasoit; Rony Marsyal Kunda; Prasetyarti Utami
Jurnal Penelitian Pendidikan IPA Vol 11 No 9 (2025): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i9.11685

Abstract

The Heat Shock Protein 70 (HSP70) gene, specifically the 3-Untranslated Region (3-UTR), is critical for regulating mRNA stability and translation under heat stress. This study aimed to identify genetic polymorphisms in the 3-UTR of the HSP70 gene in Moa buffalo (Bubalus bubalis) and evaluate its potential role in heat adaptation. A total of 65 hair follicle samples (55 Moa buffalo and 10 Banten buffalo) were collected, followed by amplification by PCR and sequencing of the PCR product of the 3'-UTR regions. Alignment results from the 3'-UTR region showed the presence of 2 major polymorphic SNPs, i.e g.1904C>A, and g.1910A>G. SNP (g.1904C>A) was found to be a cytosine (C) to adenine (A) substitution mutation type, and SNP g.1910A>G an adenine (A) to guanine (G) substitution. The discovery of 2 SNPs in the 3-UTR of the HSP70 gene in Moa buffalo is very important for the development of genetic markers, helping to understand the mechanism of heat adaptation, to the conservation and improvement of livestock performance, especially Moa buffalo living in tropical environments.
Exploration of the Potential of Hotong (Setaria italica) Based on Local Wisdom as a Functional Food for Stunting Prevention in Maluku Jamaludin; Rony Marsyal Kunda; Jilian Risky Huwae; Milda Rianty Lakoan
Jurnal Penelitian Pendidikan IPA Vol 11 No 12 (2025): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i12.13228

Abstract

Stunting remains a major nutritional problem in Indonesia, particularly in vulnerable regions such as South Buru Regency, Maluku Province. Local food diversification represents a strategic approach to improving community nutrition and food security. This study evaluated the nutritional value and socio-ecological potential of Hotong (Setaria italica), a traditional cereal cultivated by local communities in Namrole District and adapted to dryland farming systems. A quantitative descriptive method was applied through a survey of 70 Hotong-farming households in Waenewen Hamlet, Labuang Village, Namrole District, Buru Regency, combined with proximate analysis of Hotong grains based on AOAC standards. The results showed that Hotong contains ash (1.33%), protein (11.60%), fat (3.43%), crude fiber (2.20%), and carbohydrates (65.31%). This nutritional composition indicates that Hotong can contribute up to 40% of protein requirements and approximately 35% of daily iron needs for toddlers aged 1–3 years. Furthermore, 86.67% of farmers reported annual production of ≥ 4 quintals despite relying on traditional cultivation practices, highlighting the crop’s availability and sustainability. These findings demonstrate that Hotong has significant potential as a functional local food for stunting prevention, while also reflecting cultural resilience and local wisdom in maintaining food systems under dryland conditions. Integrating Hotong into regional nutrition and food security programs, including the development of locally processed food products, may contribute to improving the nutritional status of toddlers in dryland areas.
Scanning Electron Microscopy of Goniodes dissimilis Denny, 1842 (Insecta: Phthiraptera) from Domestic Chickens on Seram Island Maria Nindatu; Rony Marsyal Kunda; Mery Pattipeilohy; Yofian Anaktototy; Ventia Cika Tanikwele; Adriana Hakapaa
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.13722

Abstract

Goniodes dissimilis (Denny, 1842) is one of the chewing lice (Ischnocera: Phthiraptera) that commonly infests native chickens (Gallus gallus domesticus). The aims of this study were to describe the morphological and ultrastructural characteristics of G. dissimilis using scanning electron microscopy (SEM). The sampling of Goniodes dissimilis was conducted in Seruawan, Hatusua, and Kamarian villages on Seram Island. SEM preparation was carried out through 2.5% glutaraldehyde fixation, 0.25 M phosphate buffer post-fixation, multistage dehydration, critical-point drying, mounting on stubs, and gold coating before being observed at various magnifications. SEM results showed that G. dissimilis from Seram Island has ultrastructural characteristics, such as the head is circumfasciate with a rounded anterior margin, a complete band on the marginal carina, a pulvinus surrounding the ventral carina, and no hyaline margin. The eyes appear reduced with long ocular setae, while the temporal region has five marginal setae, with setae numbered 2 and 3 presents. The antennae are sexually dimorphic, consisting of a scape, pedicle, and three flagellomeres. In this study, we collected only females of G. dissimilis, and the first flagellomer shows no radial grooves in the sensilla placodea. The thorax shows a pteronotum with several long spines, while the abdomen shows medially separated tergums II–VIII, spiracles on tergums III and VIII, and a thin, membranous sternal plate. The SEM results also show that the female G. dissimilis has terminalia displaying a dorsal plate connected with tergopleurites and a vulva lined with fine setae. This finding represents the first ultrastructural record of the G. dissimilis population on Seram Island, thereby enriching the morphological diversity and distribution data of chewing lice in the Maluku region.
Impact of Environmental Geographic toward Point Mutations in Exon 1 of Growth Differentiation Factor (GDF9) Gene in Kosta and Lakor Goat Breeds Maman Rumanta; Rony Marsyal Kunda; Slamet Diah Volkandari; Ivan Mambaul Munir
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.3794

Abstract

This study aims to analyze the impact of environmental biogeography on point mutations in Kosta and Lakor goat breeds as local Indonesian germplasm using the GDF9 Exon 1 gene sequence. A total 43 folicle samples of Kosta and Lakor goats which collected in Serang regency and Lakor island were analyzed. To isolate the total genome of the samples, the tissue isolation method was used. The polymerase chain reaction (PCR) method was used for amplification of the exon-1 region of the GDF9 gene with two pairs of primers. Analysis of genetic variation and single nucleotide polymorphisms (SNP) using MEGA software version X. Multiple alignment results showed that there are 2 nucleotides that have mutations, but not encode different amino acids. This results indicates that the mutation has association with litter size of two breeds. This finding shows that Lakor goats have polymorphism i.e SNP g.54C>T which is significantly associated with litter size. The analysis of GDF9 gene showed that all samples from the Two local Indonesian goat breeds (Kosta and Lakor) are  monomorphic. But, although both are monomorphic, geographical isolation still contributes to genetic drift within and between populations.
Biosensors Perancangan dan Pengembangan Biosensor untuk Deteksi Kualitas Perairan Laut Berbasis Piranti Mikrokontroler ESP32: Perancangan dan Pengembangan Biosensor untuk Deteksi Kualitas Perairan Laut Berbasis Piranti Mikrokontroler ESP32 Maman Rumanta; Rony Marsyal Kunda; Fredrik Manuhutu
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.6944

Abstract

Water quality in cultivating marine organisms is a very important part to pay attention to. Prevention of pollution in marine waters needs to be done by recognizing changes in physical, chemical and biological parameters in these waters from normal conditions. To find out these changes, it is necessary to carry out measurements which include parameters that determine the quality of a liquid and carry them out periodically. The measurement parameters measured include pH, Dissolved Oxygen (DO), Electrical Conductivity (EC) and temperature. The use of technological developments, especially in the world of microcontrollers, namely the ESP32 device, makes it possible to design measurement instruments in determining the quality of sea waters to be more efficient and comprehensive. The instrument created in this research will be equipped with several sensors that will read the parameters that determine water quality. The sensors used are pH sensors, DO sensors, EC sensors and temperature sensors. This research aims to create an integrated instrument (pH, DO, ED and Temperature) with instrument performance specifications that have good accuracy and a portable design so that it is easy to use in the field. The research results show thatdevelopment of a biosensor instrument for measuring seawater quality based on the ESP32 DevKit V1 can work well and can display temperature, degree of acidity (pH), DO and EC parameters on the LCD screen according to actual conditions with a difference range in percent between 0.2% - 4.16 %. The accuracy level of the designed tool is classified as having a high accuracy above 95%
Bioaccumulation of Pb in the Gills of Perna viridis (Bivalvia: Mytilidae) in Jakarta Bay, Indonesia and its Histological Alteration Rony Marsyal Kunda; Maman Rumanta; Leonard Raden Hutasoit
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.7109

Abstract

Pollution in Jakarta Bay associated with terrestrial run-off and eutrophication. Bivalves have been recognized as aquatic organisms that play the most dominant role in aquatic ecosystems. Pb is an element that in many cases causes health problems in the liver, kidneys, hematopoietic system and nervous system. This study aims to analyze the bioaccumulation of Pb in the gills of P. viridis in Jakarta Bay, and its histological changes. Bioaccumulation analysis using the AAS and histology changes using transmission electron microscopy (TEM) methods. The results of AAS, showed that the value of Pb concentration in gill tissue at the two seasons did not show a noticeable difference. Pb concentrations in gills were lower at 1, 4, and 5 than at 2 and 3 stations. The results of ultrastructure analysis as an impact of Pb accumulation showed that thickening of the nucleus membrane, and a decrease in the number of microvilli. The thickening of the nucleus membrane, and the decrease in the number of microvilli are cellular responses to the entry of the Pb heavy metal into the cell. Besides, the results of this study showed inflammation, a decrease in the number of mitochondria, as well as causing cristae to be empty.
Preliminary PCR Screening and Optimization for Five Suspected Viruses in Haemaphysalis bispinosa Ticks from Moa Buffalo (Bubalus bubalis) Prasetyarti Utami; Rony Marsyal Kunda
Jurnal Penelitian Pendidikan IPA Vol 12 No 1 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i1.13910

Abstract

Tick-borne viruses are becoming a serious threat to livestock health, especially in tropical regions where monitoring systems are still limited. H. bispinosa, the main external parasite of water buffalo in Eastern Indonesia, is suspected of carrying several veterinarians' important viral pathogens. This study aims to detect five viruses consisting of DNA viruses (BuGyKV, BoHV-1, and OvHV-2) and RNA viruses (FMDV and TAMV). FMDV and TAMV, both RNA viruses, need to be converted into complementary DNA (cDNA) through reverse transcription, which is then used as a template for PCR amplification using virus-specific primers. All viruses were detected using specific primers and extensive annealing temperature optimization. The results showed weak and nonspecific amplification bands at one temperature for BuGyKV, BoHV-1, and OvHV-2, but these bands did not match the expected amplicon size. No amplification bands were observed for FMDV and TAMV under any conditions. These findings suggest that this amplification failure is likely due to primer mismatch with local virus variants resulting from geographical differences. Overall, it can be concluded that the five target viruses were not detected in the H. bispinosa samples examined. This preliminary study provides an initial virological overview of H. bispinosa ticks from Moa Island and emphasizes the need for more sensitive detection methods, such as nested PCR, qPCR, or metagenomic approaches, to improve the detection of low-titer viruses in ticks.
KEANEKARAGAMAN JENIS KATAK DI HUTAN ADAT AFSYA, KAMPUNG BARIAT, DISTRIK KONDA, KABUPATEN SORONG SELATAN Charissa Alvara Rajaloa; Rony Marsyal Kunda; Cornelis Karel Pattinasarany
Jurnal Sylva Scienteae Vol 9, No 3 (2026): Jurnal Sylva Scienteae Vol 9 No 3 Edisi Juni 2026
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jss.v9i3.18508

Abstract

Penelitian ini bertujuan untuk mengetahui keanekaragaman jenis katak di Hutan Adat Afsya, Kampung Bariat, Distrik Konda, Kabupaten Sorong Selatan. Pengamatan dilakukan pada Januari 2026 menggunakan metode Visual Encounter Survey (VES) pada lima transek. Analisis data menggunakan Indeks Keanekaragaman Shannon-Wiener (H’). Hasil penelitian menunjukkan 14 jenis katak dari 5 famili dengan total 126 individu. Nilai H’ berkisar 1,895–2,512 (kategori sedang), menunjukkan keanekaragaman jenis yang cukup beragam dengan penyebaran individu yang relatif merata. Penelitian ini merupakan data ilmiah pertama mengenai keanekaragaman katak di Hutan Adat Afsya dan diharapkan menjadi acuan konservasi berbasis masyarakat adat.
Co-Authors Adriana Hakapaa Charissa Alvara Rajaloa Cornelis Karel Pattinasarany Fredrik Manuhutu Fredrik Manuhutu, Fredrik Habibi Habibi Hendrina Lelloltery Hery Wijayanto Heryanus Jesajas Ivan Mambaul Munir JAMALUDIN JAMALUDIN Jamhari Jamhari Jilian Risky Huwae Lakoan, Milda Rianty Leonard R. Hutasoit Maman Rumanta Maman Rumanta Maria Nindatu Marleny Leasa Marthina Tjoa Maya M. S. Puttileihalat Mery Pattipeilohy Milda Rianty Lakoan Moniharapon, Mechiavel Niken Satuti Nur Handayani Niken Satuti Nur Handayani Pieter Kakisina Prasetyarti Utami Prasetyarti Utami Prasetyarti Utami Richard R Lokollo, Richard R Rini Widayanti Salamor, Reinhard Sandra Sukmaning Aji Selanno, Frenly M. Slamet Diah Volkandari Slamet Diah Volkandari Sri Kurniati Handayani Ucu Rahayu Utami, Prasetyarti Ventia Cika Tanikwele Woro Danur Wendo Yofian Anaktototy Yuli Tirtariandi El Anshori