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All Journal Buletin Peternakan Jurnal Veteriner Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Matematika Sains dan Teknologi Agrinimal Jurnal Ilmu Ternak dan Tanaman INDRA: Jurnal Pengabdian kepada Masyarakat Pakem : Jurnal Pengabdian Kepada Masyarakat Jurnal Teknologi Lingkungan Lahan Basah Jurnal Kedokteran Hewan
Rony Marsyal Kunda
Department Of Biology, Faculty Of Mathematics And Natural Science, Universitas Pattimura, Ambon, 97233, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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INFESTASI CAPLAK (Acari: Ixodidae) PADA KERBAU MOA DI PULAU MOA KABUPATEN MALUKU BARAT DAYA Porumau, Yandri Yanes; Kunda, Rony Marsyal; Utami, Prasetyarti
Agrinimal Jurnal Ilmu Ternak dan Tanaman Vol 13 No 1 (2025): Agrinimal Jurnal Ilmu Ternak dan Tanaman
Publisher : Jurusan Peternakan Fakultas Pertanian Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajitt.2025.13.1.57-64

Abstract

Penelitian ini bertujuan untuk mengidentifikasi jumlah, distribusi, dan preferensi infestasi caplak (famili Ixodidae) pada kerbau Moa di tiga desa di Kabupaten Maluku Barat Daya, yakni Klis, Tounwawan, dan Werwaru. Sebanyak 900 individu caplak berhasil dikoleksi dan didominasi oleh genus Haemaphysalis. Analisis infestasi berdasarkan lokasi menunjukkan bahwa Desa Klis memiliki jumlah kerbau terinfestasi tertinggi sebanyak 28 ekor, disusul oleh Desa Tounwawan dan Werwaru masing-masing dengan 15 ekor. Infestasi berdasarkan kelompok umur menunjukkan prevalensi yang signifikan lebih tinggi pada kerbau dewasa dibandingkan pedet (anak kerbau). Tidak ditemukan infestasi pada pedet di Desa Klis dan Tounwawan, sedangkan pada kelompok dewasa ditemukan masing-masing 325 dan 250 individu caplak dengan rata-rata infestasi per ekor sebesar 13 dan 12,5. Di Desa Werwaru, pedet menunjukkan infestasi ringan (rata-rata 0,38 caplak per ekor), sedangkan kelompok dewasa mengalami infestasi yang lebih berat dengan total 320 caplak (rata-rata 14,55 caplak per ekor). Berdasarkan bagian tubuh yang terinfestasi, telinga merupakan lokasi dominan dengan jumlah 369 caplak (41% dari total). Hal ini kemungkinan besar disebabkan oleh sifat telinga yang hangat, lembap, dan relatif terlindung, sehingga menjadi habitat ideal bagi caplak untuk berkembang biak. Hasil penelitian ini diharapkan dapat menjadi dasar dalam strategi pengendalian caplak yang efektif, dengan memperhatikan faktor umur, lokasi tubuh, dan distribusi spasial infestasi. ABSTRACT This study aimed to identify the number, distribution, and infestation preferences of ticks (family Ixodidae) on Moa buffalo in three villages in the Southwest Maluku Regency, i.e Klis, Tounwawan, and Werwaru. A total of 900 tick specimens were collected, predominantly from the genus Haemaphysalis. Infestation analysis based on location showed that Klis had the highest number of infested buffalo (28 individuals), followed by Tounwawan and Werwaru with 15 individuals each. Age-based infestation data revealed a significantly higher prevalence in adult buffalo compared to calves. No infestation was recorded in calves from Klis and Tounwawan, while adult buffalo in these villages were infested with 325 and 250 ticks, respectively, with an average of 13 and 12.5 ticks per animal. In Werwaru, calves showed mild infestation (average of 0.38 ticks per animal), while adult buffalo had more severe infestations with 320 ticks found (average of 14.55 per animal). Analysis of tick attachment sites on the buffalo's body revealed the ears as the most commonly infested area, with a total of 369 ticks (41% of the total count). This is likely due to the ears' warm, moist, and sheltered conditions, making them an ideal site for tick survival and reproduction. These findings are expected to serve as a basis for developing effective tick control strategies, considering factors such as age, body part preference, and spatial distribution of infestations.
Animals in Tradition and Culture: An Ethnozoological Study of the Indigenous People of Seram Island Tjoa, Marthina; Kunda, Rony Marsyal; Lelloltery, Henderina; Puttileihalat, Maya MS
Jurnal Penelitian Pendidikan IPA Vol 11 No 7 (2025): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i7.11286

Abstract

This study aims to examine the relationship between the indigenous people of Seram Island and several species of animals that are utilized for socio-cultural purposes and how the practice of ethnozoology can contribute to sustainable conservation efforts. The research was conducted using an ethnographic approach through in-depth interviews with purposively selected respondents. Qualitative descriptive analysis was conducted systematically based on themes that bind the types of animals studied. The results showed that the practice of ethnozoology includes utilization for ritual purposes related to local community belief values. Some animal species that are utilized for special purposes include Cuscuses (Phalanger spp.) and wild boar (Sus scrofa) which are useful for traditional ceremonies. The animals are killed and the meat is divided to be eaten during the ceremony. Gosong Bird (Eulipoa wallacei) as a Season Marker helps the community in managing agricultural land, marked by the presence or absence of these animals at certain locations. Deer (Rusa timorensis) as a Source of Food and Social Status by utilizing its meat. Cassowary (Casuarius spp.) as a symbol of strength. Hornbill (Rhyticeros plicatus) as a symbol of longevity. These animals are utilized by indigenous people not only for traditional rituals but also for animal protein needs such as cuscuses, Wild Boar and Deer. The needs of the indigenous community for the aforementioned animals have encouraged the community to maintain and preserve them based on their local wisdom such as the application of Sasi.
Polimorfisme Genetik dari Daerah 3'-Non Koding Region (3'-UTR) dari Gen HSP 70 pada Kerbau Moa (Bubalus bubalis) Rumanta, Maman; Hutasoit, Leonard Raden; Kunda, Rony Marsyal; Utami, Prasetyarti
Jurnal Penelitian Pendidikan IPA Vol 11 No 9 (2025): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i9.11685

Abstract

The Heat Shock Protein 70 (HSP70) gene, specifically the 3-Untranslated Region (3-UTR), is critical for regulating mRNA stability and translation under heat stress. This study aimed to identify genetic polymorphisms in the 3-UTR of the HSP70 gene in Moa buffalo (Bubalus bubalis) and evaluate its potential role in heat adaptation. A total of 65 hair follicle samples (55 Moa buffalo and 10 Banten buffalo) were collected, followed by amplification by PCR and sequencing of the PCR product of the 3'-UTR regions. Alignment results from the 3'-UTR region showed the presence of 2 major polymorphic SNPs, i.e g.1904C>A, and g.1910A>G. SNP (g.1904C>A) was found to be a cytosine (C) to adenine (A) substitution mutation type, and SNP g.1910A>G an adenine (A) to guanine (G) substitution. The discovery of 2 SNPs in the 3-UTR of the HSP70 gene in Moa buffalo is very important for the development of genetic markers, helping to understand the mechanism of heat adaptation, to the conservation and improvement of livestock performance, especially Moa buffalo living in tropical environments.
IDENTIFICATION OF GENETIC DIVERSITY CYTOCHROME OXIDASE SUBUNIT II (COII) MITOCHONDRIAL GENE AS GENETIC MARKER FOR ANISAKIS SPECIES IN Euthynnus affinis Utami, Prasetyarti; Handayani, Niken Satuti Nur; Kunda, Rony Marsyal
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.2960

Abstract

ABSTRACTThis study aimed to get specific genetic marker for Anisakis sp. identification on mackerel tuna using gene sequence cytochrome oxidase subunit II (COII) mitochondrial deoxyribonucleic acid (mtDNA) and to identify taxonomic affiliation between Anisakis sp. from Indonesia and others Anisakis sp. from GenBank database. This study started with sample collections at three fish auctions in Cilacap (Central Java), morphology classification, DNA isolation, and molecular based identification using polymerase chain reaction (PCR) and sequencing methods. Molecular based identification of Anisakis used gene amplification COII mtDNA as a cell target prior to sequence. Morphology characteristic results showed that Anisakis nematodes which infected mackerel tuna classified as type II L3 larvae. Molecular based identification showed significant result, which found 530 bp COII DNA gene fragment similar to target cell. Gene sequencing alignment results of COII Anisakis gene compared with GenBank showed 11 different nucleotide sites that can be used as genetic barcode for Indonesian Anisakis sp. This study showed that Anisakis sp. infected mackerel tuna in Java Sea is Anisakis physeteris and considered as zoonosis.
Pelatihan PELATIHAN PENGGUNAAN ALAT PRAKTIKUM IPA TERINTEGRASI PADA SISWA DAN GURU DI SMA NEGERI 2 SERAM BAGIAN BARAT Kunda, Rony Marsyal; Lokollo, Richard Rudolf; Manuhutu, Fredrik; Salamor, Reinhard; El Anshori, Yuli Tirtariandi
PAKEM : Jurnal Pengabdian Kepada Masyarakat Vol 4 No 1 (2024): Pakem : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pakem.4.1.88-93

Abstract

Pembelajaran IPA mestinya lebih menekankan pada dimensi pedagogik modern, yaitu menggunakan scientific approach. Pembelajaran yang relevan diterapkan adalah pembelajaran melalui eksperimen (praktikum). Melalui praktikum siswa memahami fenomena alam yang dikaji dari berbagai sudut sehingga siswa dapat belajar lebih bermakna (meaningful learning). Namun, kendala yang sering dihadapi yaitu kegiatan praktikum memerlukan waktu yang lama jika pembelajarannya dikemas dalam praktikum. Oleh karena itu diperlukan adanya suatu kegiatan pemberdayaan untuk meningkatkan kompetensi guru dalam melaksanakan praktikum. Kegiatan pemberdayaan ini dikemas dalam bentuk inservice training yaitu pelatihan dan pendampingan penggunaan alat praktikum IPA secara terintegrasi pada siswa dan guru di SMA. Pelatihan yang dilaksanakan adalah pelatihan mengembangkan keterampilan dan kreativitas guru IPA dalam menggunakan perangkat praktikum IPA terintegrasi yang memanfaatkan KIT IPA yang sudah diproduksi. Pendampingan juga dilakukan terkait dengan meningkatkan kemampuan guru dalam mengeksplorasi, mengelaborasi dan merefleksi materi ajar IPA. Kegiatan pelatihan dan pendampingan kepada guru dan siswa ini akan dilakukan di SMA Negeri 2 Seram Bagian Barat.
Impact of Environmental Geographic toward Point Mutations in Exon 1 of Growth Differentiation Factor (GDF9) Gene in Kosta and Lakor Goat Breeds Rumanta, Maman; Kunda, Rony Marsyal; Volkandari, Slamet Diah; Munir, Ivan Mambaul
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.3794

Abstract

This study aims to analyze the impact of environmental biogeography on point mutations in Kosta and Lakor goat breeds as local Indonesian germplasm using the GDF9 Exon 1 gene sequence. A total 43 folicle samples of Kosta and Lakor goats which collected in Serang regency and Lakor island were analyzed. To isolate the total genome of the samples, the tissue isolation method was used. The polymerase chain reaction (PCR) method was used for amplification of the exon-1 region of the GDF9 gene with two pairs of primers. Analysis of genetic variation and single nucleotide polymorphisms (SNP) using MEGA software version X. Multiple alignment results showed that there are 2 nucleotides that have mutations, but not encode different amino acids. This results indicates that the mutation has association with litter size of two breeds. This finding shows that Lakor goats have polymorphism i.e SNP g.54C>T which is significantly associated with litter size. The analysis of GDF9 gene showed that all samples from the Two local Indonesian goat breeds (Kosta and Lakor) are  monomorphic. But, although both are monomorphic, geographical isolation still contributes to genetic drift within and between populations.
Biosensors Perancangan dan Pengembangan Biosensor untuk Deteksi Kualitas Perairan Laut Berbasis Piranti Mikrokontroler ESP32: Perancangan dan Pengembangan Biosensor untuk Deteksi Kualitas Perairan Laut Berbasis Piranti Mikrokontroler ESP32 Rumanta, Maman; Kunda, Rony Marsyal; Manuhutu, Fredrik
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.6944

Abstract

Water quality in cultivating marine organisms is a very important part to pay attention to. Prevention of pollution in marine waters needs to be done by recognizing changes in physical, chemical and biological parameters in these waters from normal conditions. To find out these changes, it is necessary to carry out measurements which include parameters that determine the quality of a liquid and carry them out periodically. The measurement parameters measured include pH, Dissolved Oxygen (DO), Electrical Conductivity (EC) and temperature. The use of technological developments, especially in the world of microcontrollers, namely the ESP32 device, makes it possible to design measurement instruments in determining the quality of sea waters to be more efficient and comprehensive. The instrument created in this research will be equipped with several sensors that will read the parameters that determine water quality. The sensors used are pH sensors, DO sensors, EC sensors and temperature sensors. This research aims to create an integrated instrument (pH, DO, ED and Temperature) with instrument performance specifications that have good accuracy and a portable design so that it is easy to use in the field. The research results show thatdevelopment of a biosensor instrument for measuring seawater quality based on the ESP32 DevKit V1 can work well and can display temperature, degree of acidity (pH), DO and EC parameters on the LCD screen according to actual conditions with a difference range in percent between 0.2% - 4.16 %. The accuracy level of the designed tool is classified as having a high accuracy above 95%
Bioaccumulation of Pb in the Gills of Perna viridis (Bivalvia: Mytilidae) in Jakarta Bay, Indonesia and its Histological Alteration Kunda, Rony Marsyal; Rumanta, Maman; Hutasoit, Leonard Raden
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.7109

Abstract

Pollution in Jakarta Bay associated with terrestrial run-off and eutrophication. Bivalves have been recognized as aquatic organisms that play the most dominant role in aquatic ecosystems. Pb is an element that in many cases causes health problems in the liver, kidneys, hematopoietic system and nervous system. This study aims to analyze the bioaccumulation of Pb in the gills of P. viridis in Jakarta Bay, and its histological changes. Bioaccumulation analysis using the AAS and histology changes using transmission electron microscopy (TEM) methods. The results of AAS, showed that the value of Pb concentration in gill tissue at the two seasons did not show a noticeable difference. Pb concentrations in gills were lower at 1, 4, and 5 than at 2 and 3 stations. The results of ultrastructure analysis as an impact of Pb accumulation showed that thickening of the nucleus membrane, and a decrease in the number of microvilli. The thickening of the nucleus membrane, and the decrease in the number of microvilli are cellular responses to the entry of the Pb heavy metal into the cell. Besides, the results of this study showed inflammation, a decrease in the number of mitochondria, as well as causing cristae to be empty.
Design and Construction of a Real-Time Air Quality Monitoring System Using IoT-Based ESP32 to Strengthen Environmental Policies El Anshori , Yuli Tirtariandi; Kunda, Rony Marsyal; Manuhutu, Fredrik
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.9820

Abstract

Air quality monitoring is one of the important steps in maintaining public health and the environment. With the development of Internet of Things (IoT) technology, air quality monitoring can be done in real-time and more efficiently. This study aims to environmental policy and design of an IoT-based air quality monitoring system using the ESP32 microcontroller. This system is designed to measure air quality parameters such as CO, NO2, temperature, and humidity using factory-calibrated sensors (DFRobot) connected to the ESP32 microcontroller. Data obtained from the sensors are processed by the ESP32 and sent to a cloud server via Wi-Fi, allowing real-time monitoring via the ThingSpeak platform which can be monitored via mobile devices or the web. The results of the air quality monitoring system design show that devices using electrochemical CO and NO sensors₂and the SHT30 sensor connected to the ESP32 is capable of reading and measuring CO, NOconcentrations., temperature, and humidity with good accuracy with a sample time of ± 20 seconds. In addition, this system can be connected online with the ThingSpeak platform, allowing visualization of measurement data in graphical form in real-time. Thus, the designed system not only functions optimally in detecting air quality parameters, but also supports efficient remote monitoring through Internet of Things (IoT) technology
EKSPLORASI METAGENOMIK DARI SERANGGA BLACK SOLDIER FLY (Hermetia illucens) PENDEGRADASI SAMPAH ORGANIK DALAM UPAYA BIOREMEDIASI LINGKUNGAN irwanto; Rumanta, Maman; Kunda, Rony Marsyal
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.9926

Abstract

The Black Soldier Fly (BSF) (Hermetia illucens Linnaeus, 1758) has long been recognized as an organism used in organic waste processing through bioconversion methods. H. illucens is known to digest organic materials into nutrient sources utilized for biomass formation with the assistance of decomposer microbiota. However, research on the structure and composition of its microbiota remains limited. This study aims to identify microbiota and their structural composition in both the larval and adult fly phases, based on organic waste feeding in tropical regions. Additionally, it seeks to provide recommendations for relevant stakeholders in identifying potential environmental bioremediation agents. The research method employed is a survey study with quantitative sample analysis. The amplification process in this study uses primers from the (V1-V9) regions of the 16S rRNA gene. Data analysis is conducted using the QIIME (Quantitative Insights into Microbial Ecology) method, utilizing high-throughput sequencing community data with QIIME2 software version 3.5.3. Microbiota from the families Lactobacillaceae and Morganellaceae have been identified as dominant in larvae, while Staphylococcaceae and Bacillaceae dominate in adult flies. Morganella morganii, Herbaspirillum piri, Dysgonomonas capnocytophagoides, and Clostridium intestinale are potential candidates for organic waste bioremediation from BSF larvae. Meanwhile, Sphingobacterium wenxiniae, Lachnoclostridium phytofermentans, Mammaliicoccus sciuri, and Corticicoccus populi are bioremediation candidates from BSF flies. The genera Enterococcus, Morganella, and Dysgonomonas are found in both temperate and tropical climate regions. However, Providencia, Klebsiella, Scrofimicrobium, and Actinomyces, which are found in the gut of BSF larvae in temperate regions, are absent in BSF larvae from tropical Indonesia. Conversely, Limosilactobacillus, Entomomonas, Lachnoclostridium, and Clostridium are not found in the gut of BSF larvae in temperate regions.
Co-Authors El Anshori , Yuli Tirtariandi Fredrik Manuhutu, Fredrik Habibi Habibi Hendrina Lelloltery Hery Wijayanto Heryanus Jesajas Hutasoit, Leonard Raden IRWANTO Jamhari Jamhari Lakoan, Milda Rianty Lokollo, Richard R. Lokollo, Richard Rudolf Maman Rumanta Marleny Leasa Maya M. S. Puttileihalat Moniharapon, Mechiavel Munir, Ivan Mambaul Niken Satuti Nur Handayani Niken Satuti Nur Handayani Pieter Kakisina Porumau, Yandri Yanes Prasetyarti Utami Rini Widayanti Salamor, Reinhard Sandra Sukmaning Aji Selanno, Frenly M. Slamet Diah Volkandari Slamet Diah Volkandari, Slamet Diah Sri Kurniati Handayani Tjoa, Marthina Ucu Rahayu Utami, Prasetyarti Woro Danur Wendo Yuli Tirtariandi El Anshori