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All Journal JBIO: jurnal biosains (the journal of biosciences) Jurnal Pelita Pendidikan Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Jurnal Biologi dan Pembelajarannya (JBP) JURNAL PENELITIAN SAINTIKA Biology, Medicine, & Natural Product Chemistry JURNAL PENGABDIAN KEPADA MASYARAKAT Al Qodiri : Jurnal Pendidikan, Sosial dan Keagamaan EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA LENSA (Lentera Sains): Jurnal Pendidikan IPA JURNAL PENDIDIKAN MIPA Biopendix: Jurnal Biologi, Pendidikan dan Terapan IJOEP : International Journal of Ecophysiology Didaktik : Jurnal Ilmiah PGSD STKIP Subang Keluwih: Jurnal Kesehatan dan Kedokteran QISTINA: Jurnal Multidisiplin Indonesia Aurelia: Jurnal Penelitian dan Pengabdian Masyarakat Indonesia BIO-CONS: Jurnal Biologi dan Konservasi Jurnal Pengabdian Masyarakat dan Riset Pendidikan Metamorfosa: Journal of Biological Science Open Access DRIVERset
Endang Sulistyarini Gultom
Department Of Biology, Universitas Negeri Medan
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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AUDIO VISUAL MEDIA DESIGN FOR PRACTICAL MICROBIOLOGY LEARNING Aryeni Aryeni; Endang Sulistyarini Gultom; Qisthy Adha Fajriati; Nila Zusmita Wasni
Jurnal Pelita Pendidikan Vol 11, No 2 (2023): Jurnal Pelita Pendidikan
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpp.v11i2.44979

Abstract

This study aims to describe the process of making audio-visual media and to determine the feasibility of it based on the assessment of material experts, media experts and student responses. This research is a descriptive study that adopts the ADDIE development step which has five stages. However, this research is limited to only three stages, the analysis, design, and development stages. The population used in this study were all majoring students of Biology in the 2018 at Unimed and the sample of this study were 40 majoring students of Biology in the 2018 who had taken microbiology courses at Unimed. Theinstrument used in this study was a questionnaire using a Likert scale. The results showed that videos of sterilization and media making, videos of observing bacterial colonies and counting the number of bacteria using the pour plate dilution method, isolation videos of pure bacteria cultures, videos of bacterial staining, videos of bacterial biochemical activity, and video of testing of antibacterial activity on sponge symbionts against bacteria MDR (Multi Drug Resistance) is categorized as very feasible based on the assessment of material experts, media experts and student responses.
Development of Student Worksheet Based on Guided Inquiry Using Articulate Storyline in Fungi Subject Matter for Tenth Grade at SMAN 2 Plus Panyabungan Fitri Afifah Nst; Endang Sulistyarini Gultom
Jurnal Pelita Pendidikan Vol 11, No 2 (2023): Jurnal Pelita Pendidikan
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpp.v11i2.38919

Abstract

This study aims to determine the feasibility of worksheets based on guided inquiry using Articulate storyline on fungi material. This study uses the ADDIE development model which consists of five steps, namely analysis, design, development, implementation, and evaluation. The results showed that teachers and students needed worksheet so that the worksheet  was designed based on guided inquiry. The validation of material experts, learning experts, and design experts are in the very good category with an average score of 96.87%, 96.87%, and 88.46%. The biology teacher's assessment obtained an average of 98.4% which was included in the very good category, and the student response to the worksheet obtained an average of 96.07% which was included in the good category. The product developed is effective in achieving learning indicators on fungi subject matter in terms of the N-gain test with a medium category of 0.65%. So, it can be concluded that worksheet is suitable for use in the learning process.
Uji Aktivitas Antibakteri Dari Ekstrak Daun Sirih Terhadap Bakteri Bacillus cereus Hasibuan, Ali Ahmatul Umri; Br Tarigan, Grace Amelia; Ulfah Rambe, Khadra; Tarigan, Sisilia Anabina; Sulistyarini Gultom, Endang
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 11 No 1 (2024): APRIL 2024
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v11i1.21662

Abstract

This study elucidates the role of secondary metabolites in green betel leaves (Piper betle) as antibacterial agents against Bacillus cereus, a bacterium causing various foodborne illnesses in humans. The experimental research was conducted at the Biology Laboratory, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, utilizing Bacillus cereus bacterial isolates and extracting betel leaves using water. The measurement of inhibition zones was performed to evaluate the antibacterial activity of the betel leaf extract. The research results indicate the presence of an inhibition zone against Bacillus cereus growth, albeit categorized as weak. The measurements varied across repetitions, namely 2.6 mm, 3.35 mm, and 1.05 mm. The average of these three results was 3 mm.
Screening of Extract Secondary Metabolites of Bacteria Which Have Symbiosis with Sponges from Central Tapanuli Bakar Island as an Antibacteria mirnawati, mirnawati; Rasyidah; Mayasari , Ulfayani; Gultom, Endang Sulistyarini
International Journal of Ecophysiology Vol. 6 No. 2 (2024): International Journal of Ecophysiology
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/ijoep.v6i1.17821

Abstract

This study aims to determine the presence of antibacterial-producing bacteria from sponges from Bakar Island, Central Tapanuli Regency and to determine the antibacterial activity of methanol extracts from potential bacterial isolates against Staphylococcus aureus and Escherichiacoli. 21 pure isolates were found and it was known that 9 isolates produced antibacterial against S. aureus and 4 isolates against E. coli. Bacterial isolates with the highest inhibition zone against bothS.aureus and E. coli were found in isolates with code SP20 with each large inhibition zone are 14.5 mm and 16.04 mm. Then microscopic observations were carried out, namely gram staining and were obtained 8 isolates were gram positive, and 1 gram was negative. The biochemical test showed that SP20 showed positive results for the Motility, TSIA and Catalase Test but negative for Citrate Test. Bacterial isolates withthe code SP20 had the largest inhibition zone so that these isolates were to be extracted.The resultsof the screening test showed that the secondary metabolitesof the sponge symbiont bacterial extract from Bakar Island were positive for flavonoids and saponins. And then test the activity of secondary metabolites with 3 concentrations, 10%, 20%, and 30%.The results showed that the best concentration of the test bacteria was S. aureus at a concentration of 30% with an inhibition zone of 3.08 mm and E. coli at 5.04 mm.
FIG ENDOPHYTIC BACTERIA PRODUCE ENZYMES AMYLASE, LIPASE, PROOTEASE, CELLULASE Habsari, Lilis; Gultom, Endang Sulistyarini; Nasution, Mhd Yusuf; Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.52820

Abstract

The use of enzymes in Indonesia is increasing every year, so action is needed to harmonize the production and needs of enzymes in Indonesia. Enzymes can be produced from various sources such as plants, animals and microorganisms. The advantage of utilizing microorganisms in enzyme production is that they can be produced in large quantities with a shorter time and can be produced continuously. One of the sources of bacterial screening to produce enzymes is figs. This study aims to look at the extracellular enzyme activity of fig endophytic bacteria and identify endophytic bacteria capable of producing extracellular enzymes such as proteases, amylase, cellulase and lipase. Isolation and purification of bacteria using NA media for 24 hours. Test of extracellular enzyme activity using NA media enriched with amylum, tween 80, skim milk, CMC. Biochemical tests are performed to identify endophytic bacteria of figs producing extracellular enzymes. The results of the study were 21 isolates of fig endophytic bacteria, as many as 14 isolates were able to produce extracellular enzymes. The identification results of fig endophytic bacteria producing extracellular enzymes obtained 8 isolates suspected to be from the genus Bacillus, 3 isolates suspected to be from the genus Micrococcus, 1 isolate suspected to be from the genus Cellulomonas, 2 isolates suspected to be from the genus Acetobacter
UJI AKTIVITAS ANTI BAKTERI MADU TRIGONA TERHADAP BAKTERI Escherichia coli dan Klebsiella pneumoniae Gultom, Endang Sulistyarini; Pohan, Selvia Dewi; Situmorang, Nurbaity; Hafzari, Rini
Eksakta : Jurnal Penelitian dan Pembelajaran MIPA Vol 10, No 1 (2025): Eksakta : Jurnal Penelitian dan Pembelajaran MIPA
Publisher : Fakultas Keguruan Dan Ilmu Pendidikan, UM-Tapsel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/eksakta.v10i1.299-304

Abstract

Antibiotic resistance has the greatest impact on human health, at least 2 million people are infected by antibiotic-resistant bacteria. The use of natural medicines is increasingly in demand by the public, one of which is often used to treat diseases is trigona honey. This study aims to determine the antibacterial activity of Trigona honey against Escherichia coli and Klebsiella pneumoniae bacteria. The test bacteria used were Multidrug Resistant Escherichia coli and Klebsiella pneumoniae bacteria obtained from the collection of the Microbiology Laboratory of the University of North Sumatera Hospital. Antimicrobial activity testing was carried out using the hole / well diffusion method on Mueller Hinton Agar (MHA) media with chloramphenicol as a positive control, acetone as a negative control and trigona honey with a concentration of 500 ppm. Antibacterial activity was seen by measuring the inhibition zone formed. The results showed that Trigona honey was able to inhibit the growth of Escherichia coli and Klebsiella pneumoniae bacteria with inhibition zone activity included in the weak category, while the positive control showed antibacterial activity in the strong category.
Profil Pertumbuhan dan Produksi Mutan Bawang Putih (Allium sativum L.) Kultivar Doulu Generasi MV5 Marbun, Jumita Sari; Gultom, Endang Sulistyarini
BIO-CONS : Jurnal Biologi dan Konservasi Vol. 7 No. 1 (2025): BIO-CONS: Jurnal Biologi dan Konservasi
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31537/biocons.v7i1.2291

Abstract

Penelitian ini  bertujuan untuk mengetahui pengaruh radiasi sinar gamma terhadap pertumbuhan dan produksi dari tanaman bawang putih (Allium sativum L.) kultivar Doulu pada generasi Mutasi Vegetatif ke 5. Penelitian ini dilakukan selama 4 bulan dari tanggal 3 September 2022 - 2 Januari 2023. Penelitian ini dilaksanakan di Desa Sionggang Utara, Kabupaten Toba Samosir, Sumatera Utara menggunakan 240 sampel siung bawang putih (Allium sativum L.) kultivar Doulu generasi MV4. Rancangan yang digunakan dalam penelitian ini yaitu Rancangan Acak Kelompok (RAK), menggunakan uji AVONA dan memakai uji lanjut BNJ 5%. Dosis perlakuan dari sinar gamma ini adalah G0 (kontrol), G1 (2 Gy), G2 (4 Gy), G3 (6 Gy), G4 (8 Gy), dan G5 (10 Gy). Parameter yang diamati yaitu umur tumbuh, jumlah daun, diameter umbi, berat umbi, jumlah siung, berat siung, dan diameter siung. Susunan daun pada tanaman bawang putih sama yaitu rendah atau low. Struktur umbi bawang putih nya regular two-fan groups, regular multi-cloved radial, regular multi-fan groups, dan irregular. Pertumbuhan bawang putih pada dosis 4 Gy yang lebih baik untuk digunakan. Sedangkan untuk umur tumbuh (4 Gy), jumlah daun (10 Gy), diameter umbi (4 Gy), berat umbi (4 Gy), jumlah siung (8 Gy), berat siung (4 Gy), dan diameter siung (4 Gy). 
Identification of Secondary Metabolites of Kirinyuh Leaves (Chromolaena odorata L.) as Antibacterial Using GS-MS grasella, martha; Sulistyarini Gultom, Endang
JURNAL BIOSAINS Vol. 11 No. 1 (2025): JURNAL BIOSAINS
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v11i1.64589

Abstract

This study aims to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against pathogenic bacteria and to determine secondary metabolites in kirinyuh leaves (Chromolaena odorata) using the GC-MS (Gas Chromatography-Mass Spectroscopy) method. The test bacteria used were Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. The extraction method was carried out by maceration using 96% ethanol solvent. The crude ethanol extract of kirinyuh leaves in this study used concentrations of 30%, 40%, 50%, 60% and 70%. Using a UV-VIS spectrophotometer, the most effective concentration in inhibiting and killing the growth of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa bacteria was a concentration of 70%, with an average OD (absorbance) value of -0.2029, -0.055, and -0.0523. Then secondary metabolite identification was carried out using GC-MS (Gas Chromatography-Mass Spectroscopy) ethanol-water (9:1). The test results showed that a concentration of 70% was the most effective in inhibiting and killing Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa bacteria. The content of secondary metabolites in the ethanol extract of kirinyuh leaves contains 33 secondary metabolite compounds, 6 of which are dominant compounds as antibacterials.
IMPLEMENTATION OF LEARNING BASED ON E-WORKSHEETS WITH A SCIENTIFIC APPROACH ON THE MATERIAL OF THE DIGESTIVE SYSTEM IN CLASS XI SMA UNGGULAN AL-MANAR Gultom, Endang Sulistyarini; Yenda, Azura Nur
Jurnal Pelita Pendidikan Vol. 13 No. 1 (2025): JURNAL PELITA PENDIDIKAN
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to determine the application of e-worksheet with a scientific approach in class XI IPA SMA Unggulan Al-Manar in the learning process as measured by students' critical thinking skills. The instrument for measuring the question is administered both before and after the implementation of the learning. The data analysis technique employs quantitative analysis, specifically the calculation of the average value, to assess the students' critical thinking skills. The results of the data analysis indicate that the implementation of learning using e-worksheet with a scientific approach can enhance the critical thinking skills of students in class XI IPA at SMA Unggulan Al-Manar. This is evidenced by the change in the cognitive value of students during the pretest and posttest, which fell within the very good category. The average value of students during the pretest was 54.13, while the average value increased to 86.75 during the posttest, indicating an improvement in critical thinking skills.
Identification of Bacterial Symbiont Sponge with Antibacterial Activity against Multi Drug Resistant Organism (MDRO) Bacteria from Sea Waters in Sibolga, North Sumatra Indonesia Gultom, Endang Sulistyarini; Hasruddin, Hasruddin; Sitompul, Aida Fitriani; Situmorang, Albert D; Prasetya, Eko
Biosfer: Jurnal Tadris Biologi Vol 12 No 2 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v12i2.10138

Abstract

This research employed the experimental method with descriptive data analysis. It aimed to isolate and identify sponge symbiont bacteria with antibacterial potential against Multi-Drug Resistance Organisms (MDRO) with the 16S rRNA gene marker. Based on the study results, two sponges were obtained, namely Clathrina sp and Agelas sp, each having 11 and 7 isolates of symbiont bacteria, respectively. Based on the antibacterial activity test results, out of 18 isolates of symbiont bacteria, only 4 had antibacterial potential against MDRO (Klebsiella pneumoniae ESBL, Pseudomonas aeruginosa ESBL, or Staphylococcus lugdunensis MRSA) with inhibition zones of 9.3 mm, 8.3 mm, and 8.1 mm, respectively. The results of bacterial identification using the 16S rRNA gene sequence isolate S1I3, S1I9, and A1I3 belong to the Bacillus cereus and Bacillus paramycoides types. This research is expected to provide important information about sponge symbiont bacteria with antibacterial potential against MDRO.
Co-Authors Adinda Laura Munthe Adinda Rizky Pratiwi Agustina Matontang Ahmad Shafwan S. Pulungan Aida Fitriani Sitompul Albert D Situmorang Alyvia Salsabila Alztara, Salsha Amelia Sasmita Br Sianturi Aryeni Aryeni Astrida Joice Angelica Batubara Atika Khovivah Baringbing, Siska Juliana Bazura, Hughes Br Tarigan, Grace Amelia Dalimunthe, Aina Mardiah Damaiyani, Damaiyani Deka Wira Pratama Barus Denisa, Rifka Dewi Peronika Hutasoit Efimah Sidabutar Eko Prasetya Eko Prasetya, Eko Elisabet, Melfa Romanna Ester Ester Fadilla, Raudatul Fanny Hafifa Fauziah, Ennita Fitri Afifah Nst Ginting, Silvia Margaretta br grasella, martha Greace Enita Purba Gultom, Wirda Resinta Habsari, Lilis Harahap, Armansyah Maulana Hasibuan, Ali Ahmatul Umri Hasnaul Maritsa Hasruddin Helen Veronita Hutapea Hutasoit, Dewi Peronika Jayawarsa, A.A. Ketut Kaban, Hairen Br Lumbantoruan, Lamtiurma Lydia Siburian Manda, Yutha Nadiroh Manullang, Hanna Mawarni Manurung, Dhea Amelia Marbun, Jumita Sari Martina Restuati Mayasari , Ulfayani Mustasfa, Sinta Wulida Mutia, Diska Qolbu Mutiara Sakinah Nafisa, Katri Nainggolan, Tiara Najwa Sarip Nasution, Ayyu Rizkia Nasution, Mhd Yusuf Ndaha, Gebyta Yani Nila Zusmita Wasni Ninta Dumaria Matanari Nur hasanah Nura S, Alfi Nurbaity Situmorang Odor Debora Pita Uli Sinaga Padang, Enmia Septia Pakpahan, Amkani Angelita Pandiangan, Putri Patricia Pasaribu, Maria Benedikta Priawan, Indra Pulungan, Ahmad Shafwan S Purba, Melpa Yusmawati Qisthy Adha Fajriati Rangkuti, Marlinda Nilan Sari Rasyidah Reni Anggraini Rini Hafzari RR. Ella Evrita Hestiandari Salsabilah, Talita Saragih, Elsa Angelina Saragih, Putri Fransiska Sari, Vio Joana Selvia Dewi Pohan Shela Estica Saragi Sihotang, Joseph Raja Oloan Siregar, Sania Situmorang, Albert D Situmorang, Nurbaity Susanto, Rafli Syarief Saadillah Lubis Tamba, Gelora Betania Tania Valentina Pasribu Tarigan, Sisilia Anabina Tri Hartanti Ulfah Rambe, Khadra Uswatun Hasanah Widyanti Saragi Wijaya, Ulfah Rianda wulandari, atika Yenda, Azura Nur Zain, Ulfi Khairani