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Nurbaity Situmorang
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PERBANDINGAN UJI DIAGNOSTIK GENEXPERT MTB/RIF DENGAN KULTUR SENSITIVITAS ANTIBIOTIK DALAM MENDETEKSI RESISTENSI RIFAMPICIN Mycobacterium tuberculosis PADA PASIEN TB PARU DI RSUP H. ADAM MALIK MEDAN Nurbaity Situmorang; Perry Boy Chandra Siahaan; Suharsih; Dewi Novina Sukapiring
Jurnal Biogenerasi Vol. 7 No. 2 (2022): Jurnal Biogenerasi Vol 7 Nomor 2 tahun 2022
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v7i2.1912

Abstract

WHO recommends GeneXpert Mycobacterium tuberculosis; Rifampicin as an initial examination for the diagnose of drug-resistant tuberculosis and drug-sensitive tuberculosis in pulmonary TB patients. The GeneXpert MTB/RIF examination is a molecular examination method with Nucleic Acid Amplification Technology (NAAT) which can diagnose TB and resistance to Rifampicin within 2 hours. The aims of this study is to determine the comparison of GeneXpert MTB/Rif Diagnostic Test with Drug Sensitivity Culture in Detecting Rifampicin M. tuberculosis resistance in TB patients at H. Adam Malik Hospital, Medan. The sample in this study was the entire population of suspected pulmonary TB at H. Adam Malik Hospital Medan who had MTB examination results. Post; rif. res. The sample size was using total sampling, namely the sampling technique where the number of samples was the same as the population, namely 128 patients in 2020. The results of the Rifampicin TCM Antibiotic Test were found that 128 people had Rifampicin Resistant results. Based on the percentage of the results of the MGIT Antibiotic Sensitivity Test, 125 people were found to have Rifampicin Resistant results and 3 people had Rifampicin Sensitive results. Based on the results, it can be seen that the sensitivity value of the TCM tool to DST MGIT culture is 97.7%. The Conclusions of these Research is the ability of the GeneXpert MTB/Rif method to detect rifampin resistance can be used as a screening tool to diagnose rifampin-resistant TB.
Peningkatan Literasi Siswa SD Negeri 106204 Tanjung Raja Melalui Pojok Baca Hendra; Nuri Ramadhan; Leni Malinda; Emy Hariati; Sisilia Florina Yanti; Nurbaity Situmorang; Dewi Novina Sukapiring; Hendri Irawan; Muhammad Khaibar Putra Adhitia; Nurul Ajima Ritonga; Astria Ayu Ramadianti; Syarifah Ainun Harahap
Jurnal Pengabdian Masyarakat Vol. 2 No. 1 (2023): Juni 2023
Publisher : Unity Academy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70340/japamas.v2i1.57

Abstract

With children's interest in reading still low, the government launched the School Literacy Movement (GLS). This program is expected to foster interest in reading in students as well as to build a literacy climate in schools. However, not all schools have been able to implement GLS in accordance with the guidelines provided. The problems include inadequate facilities and infrastructure, limited reading materials, limited reading time. The purpose of this community service is to develop reading literacy in students at UPT Elementary School in launching GLS through books and reading trees provided by the PKM team to each partner school. Activities in assisting the making of this class reading corner are carried out by the team and class teachers at the State Elementary School, starting from preparing the class that will later be made a class reading corner, the materials needed in making the reading corner, the books to be donated, and the teacher's role in mentoring later. The team will also donate basic education level books and folklore books to fulfill the bookshelves that will be made in the reading corner later. The end result of this activity is expected that the reading corner that has been made will be emulated by teachers in other classes and the donated books will be useful for teachers and students and schools.
SOSIALISASI MENCUCI TANGAN DENGAN BENAR DAN PELATIHAN PEMBUATAN PAPER SOAP DI SMP NEGERI 1 SORKAM, KECAMATAN SORKAM KABUPATEN TAPANULI TENGAH Emy Hariati; Hendra; Nuri Ramadhan; Leni Malinda; Sisilia Florina Yanti; Nurbaity Situmorang; Dewi Novina Sukapiring; Mastari Ramadhani; Juhardi Sembiring; Rizky Ananda; Fatimah Pohan; Suharsih; Syarifah Ainun Harahap; Rara Astili Siregar
Jurnal Pengabdian Masyarakat Vol. 3 No. 1 (2024): Juni 2024
Publisher : Unity Academy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70340/japamas.v3i1.117

Abstract

Regular handwashing is the first 5M health protocol and is effective in preventing the transmission of viruses and diseases. However, mobility and activities cause many to neglect to always have soap on hand when travelling. For this reason, a simple innovation for the use of soap is needed, namely by using paper soap. Paper soap is much more practical in its use and easy to carry anywhere. Based on the survey of the service team, several problems were found, such as students who were often absent from school due to illness, students' low understanding of hand washing with soap, and students' lack of knowledge in making paper soap. The purpose of this community service is to improve students' knowledge and skills in applying the six steps of handwashing with soap and to improve students' skills in making paper soap with natural ingredients. This community service was carried out by providing counselling on the importance of hand washing, assistance in making paper soap from natural ingredients to students. The results obtained from this service are an increase in knowledge and creativity of students who initially did not know how to make paper soap to know, and increased student understanding of the steps of good and correct hand washing.
Penerapan Aspek Bioetika dalam Penggunaan Pestisida Nabati terhadap Pencegahan Hama Lingkungan: Studi Kasus di UPT Pengembangan Benih Hortikultura Dinas Ketahanan Pangan Pertanian dan Perikanan Kota Medan Bunga Ria Sitanggang; Nur Afliza; Rika Lidia Sibarani; Ahmad Shafwan Pulungan; Nurbaity Situmorang
Jurnal Riset Rumpun Ilmu Tanaman Vol. 4 No. 1 (2025): April : Jurnal Riset Rumpun Ilmu Tanaman
Publisher : Pusat riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrit.v4i1.4317

Abstract

The study explores the application of bioethical aspects in the use of botanical pesticides for pest prevention in the environment at the Horticultural Seed Development Unit (UPT) under the Department of Agriculture and Fisheries. Utilizing a qualitative approach with quantitative elements, this research aims to deepen understanding regarding bioethical practices in botanical pesticide usage and evaluate its effectiveness in controlling pests. The population consists of employees and students involved in pesticide-related activities, selected through purposive sampling to ensure knowledgeable respondents. Key variables include the independent variable of botanical pesticide application and dependent variables such as pest population reduction, environmental impact, and the presence of beneficial non-target organisms like pollinators. The descriptive qualitative method combined with survey techniques provides insights into how bioethical principles are integrated into pest control strategies, promoting sustainable agricultural practices. This research contributes to advancing environmentally friendly pest management solutions while maintaining ecological balance.
KAJIAN ETIKA DALAM PENGGUNAAN BAHAN ORGANIK DAN ANORGANIK PADA MEDIA KULTUR JARINGAN ANGGREK Habil Akbar; Nurbaity Situmorang; Rizka Juliana Siregar; Widya Purnama Sitanggang
Jurnal Saintifik (Multi Science Journal) Vol 23 No 2 (2025): MEI
Publisher : Fakultas Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58222/js.v23i2.443

Abstract

Kultur jaringan anggrek merupakan teknik perbanyakan tanaman yang penting, namun penggunaan bahan organik dan anorganik dalam media kultur menimbulkan permasalahan etika terkait keberlanjutan dan dampak lingkungan. Penelitian ini bertujuan untuk menganalisis penerapan prinsip etika dalam penggunaan bahan organik (seperti ekstrak pisang, air kelapa) dan anorganik (seperti garam mineral, ZPT sintetik) pada media kultur jaringan anggrek. Dampak penggunaan kedua jenis bahan terhadap lingkungan dan keseimbangan ekosistem juga dikaji. Selain itu, penelitian ini bertujuan untuk menemukan solusi optimal dalam pembuatan media kultur jaringan anggrek yang efisien, ramah lingkungan, dan berkelanjutan. Diharapkan hasil penelitian ini dapat memberikan pemahaman tentang prinsip etika, potensi dampak lingkungan, dan alternatif media kultur yang lebih berkelanjutan bagi industri dan konservasi tanaman anggrek. Penelitian ini dilakukan dengan studi pustaka dan analisis mendalam terhadap berbagai penelitian terkait, serta mempertimbangkan aspek bioetika dalam pemilihan bahan media kultur.
Penerapan Prinsip Bioetika dalam Pemeriksaan Kualitas Air (Pb dan Cd) dengan Metode AAS (Atomic Absorption Spectro-Photometer): (Studi Kasus di UPT Laboratorium Pengujian Mutu dan Keamanan Pangan Kota Medan) Nita Maya Sari Pane; Zihan Zahriani Batubara; Ahmad Shafwan Pulungan; Nurbaity Situmorang
Jurnal Pendidikan Kimia, Fisika dan Biologi Vol. 1 No. 2 (2025): Maret : Jurnal Pendidikan Kimia, Fisika dan Biologi
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/jupenkifb.v1i2.225

Abstract

Current water safety issues in society are very diverse. Water quality safety issues are closely related to the bioethics of water testing, because water testing involves protecting human health and the environment. Water quality testing is an important part of ensuring that the water consumed is safe for human health. The purpose of this study was to evaluate the application of bioethical principles in water quality testing (Pb and Cd). This study was conducted in March 2025 at the UPT Food Security Laboratory of Medan City. The data analysis used in this study used qualitative descriptive methods to see the pattern of observation responses. The results of the study showed that in the bioethical aspect of sample testing, analysts who conducted the testing had implemented bioethics or SOPs that apply in food quality and safety testing laboratories. In conducting water testing for lead and cadmium examination, the procedures used were in accordance with applicable requirements using testing procedures from SNI (Indonesian National Standard).
Pemanfaatan Ekstrak Etanol Daun Bawang Batak (Allium chinense G. Don) Sebagai Antibakteri Sari, Vio Joana; Adinda Rizky Pratiwi; Amelia Sasmita Br Sianturi; Fanny Hafifa; Najwa Sarip; Endang Sulistyarini Gultom; Nurbaity Situmorang
Al Qodiri : Jurnal Pendidikan, Sosial dan Keagamaan Vol. 23 No. 3 (2025): Al Qodiri : Jurnal Pendidikan, Sosial dan Keagamaan (In Progress)
Publisher : Lembaga Penelitian, Pengabdian kepada Masyarakat dan Publikasi Ilmiah (LP3M) Institut Agama Islam (IAI) Al-Qodiri Jember, Jawa Timur Indonesia bekerjasama dengan Kopertais Wilayah 4 Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53515/qodiri.2025.23.3.587-597

Abstract

Abstract: This study aimed to evaluate the antibacterial activity of the ethanol extract of Batak onion leaves (Allium chinense G. Don) against soil bacteria using the disk diffusion method. The treatments included ethanol extract of Batak onion leaves, chloramphenicol as a positive control, and dimethyl sulfoxide (DMSO) as a negative control. The results showed that the ethanol extract exhibited antibacterial activity with an average inhibition zone of 3.16 mm, categorized as weak antibacterial activity. In contrast, the positive control (chloramphenicol) produced an inhibition zone of 28.07 mm, indicating very strong antibacterial activity, while the negative control (DMSO) showed no inhibition zone. These results confirm that the observed antibacterial effect originated from the active compounds in the extract or antibiotic rather than the solvent. The antibacterial potential of A. chinense G. Don is attributed to the presence of secondary metabolites such as flavonoids, saponins, and terpenoids, which can damage bacterial cell walls or inhibit protein synthesis. Therefore, although the inhibitory effect is relatively weak, Batak onion leaves have promising potential as a natural antibacterial source with further optimization of extract concentration and purification methods. Keywords: Allium chinense G. Don, Antibacterial Activity, Ethanol Extract, DMSO, Chloramphenicol, Disk Diffusion Method Abstrak: Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun bawang Batak (Allium chinense G. Don) terhadap bakteri tanah dengan menggunakan metode uji kertas cakram (disk diffusion). Perlakuan yang digunakan meliputi ekstrak etanol daun bawang Batak, antibiotik kloramfenikol sebagai kontrol positif, dan dimetil sulfoksida (DMSO) sebagai kontrol negatif. Hasil pengujian menunjukkan bahwa ekstrak etanol daun bawang Batak memiliki daya hambat terhadap bakteri tanah dengan rata-rata zona hambat sebesar 3,16 mm, tergolong dalam kategori aktivitas antibakteri lemah. Sebaliknya, kontrol positif (kloramfenikol) menghasilkan zona hambat sebesar 28,07 mm dengan aktivitas antibakteri sangat kuat, sedangkan kontrol negatif (DMSO) tidak menimbulkan zona hambat sama sekali. Hasil tersebut menunjukkan bahwa aktivitas antibakteri yang dihasilkan murni berasal dari zat aktif ekstrak maupun antibiotik. Aktivitas antibakteri pada ekstrak daun bawang Batak diduga berasal dari kandungan senyawa metabolit sekunder seperti flavonoid, saponin, dan terpenoid yang bekerja dengan cara merusak dinding sel bakteri atau menghambat sintesis protein. Dengan demikian, meskipun daya hambatnya masih lemah, daun bawang Batak berpotensi dikembangkan sebagai sumber antibakteri alami melalui optimasi konsentrasi dan metode ekstraksi yang lebih efektif. Kata Kunci: Allium chinense G. Don, Aktivitas Antibakteri, Ekstrak Etanol, DMSO, kloramfenikol, Kertas Cakram
Uji Efektivitas Ekstrak Etanol Moringa oleifera L. terhadap Pertumbuhan Escherichia coli Endang Sulistyarini Gultom; Nurbaity Situmorang; Adinda Laura Munthe; Agustina Matontang; Alyvia Salsabila; Deka Wira Pratama Barus; Lydia Siburian; Widyanti Saragi
Keluwih: Jurnal Kesehatan dan Kedokteran Vol. 7 No. 1 (2025): Keluwih: Jurnal Kesehatan dan Kedokteran (December) - In Progress
Publisher : Direktorat Penerbitan dan Publikasi Ilmiah, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/kesdok.V7i1.8012

Abstract

Abstract—Escherichia coli. is a Gram-negative bacterium that commonly lives as normal flora in the digestive tract however, certain strains can cause serious diseases such as diarrhea and urinary tract infections. The high rate of antibiotic resistance has encouraged the search for alternative treatments based on natural ingredients. Moringa (Moringa oleifera L.) leaves are known to contain bioactive compounds such as flavonoids, tannins, saponins, and alkaloids, which have potential antibacterial properties. This study aimed to determine the effectiveness of ethanol extract of Moringa leaves against the growth of E. coli using the disc diffusion method on Eosin Methylene Blue Agar(EMBA) medium. The treatments consisted of three extract concentrations—55%, 75%, and 95%—along with a positive control (chloramphenicol) and a negative control (96% ethanol). The results showed that the higher the extract concentration, the larger the inhibition zone diameter formed, namely 7.68 mm at 55%, 9.85 mm at 75%, and 10.86 mm at 95%. ANOVA analysis indicated a significant difference among treatments (p < 0.001). The antibacterial activity of Moringa leaf ethanol extract was categorized as moderate to strong and bacteriostatic, meaning it inhibits bacterial growth without completely killing the bacteria. Therefore, the ethanol extract of Moringa leaves has the potential to be developed as a natural antibacterial agent against Escherichia coli. Keywords: antibacterial, ethanol extract, Escherichia coli., inhibition, Moringa oleifera L., Abstrak—Escherichia coli. merupakan bakteri Gram negatif yang umumnya hidup sebagai flora normal di saluran pencernaan, namun beberapa strainnya dapat menyebabkan penyakit serius seperti diare dan infeksi saluran kemih. Tingginya angka resistensi antibiotik mendorong perlunya pencarian alternatif pengobatan berbasis bahan alami. Daun kelor (Moringa oleifera L.) diketahui mengandung senyawa bioaktif seperti flavonoid, tanin, saponin, dan alkaloid yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol daun kelor terhadap pertumbuhan E. coli menggunakan metode difusi cakram pada media Eosin Methylene Blue Agar (EMBA). Perlakuan terdiri atas tiga konsentrasi ekstrak, yaitu 55%, 75%, dan 95%, serta kontrol positif (kloramfenikol) dan kontrol negatif (etanol 96%). Hasil penelitian menunjukkan bahwa semakin tinggi konsentrasi ekstrak, semakin besar diameter zona hambat yang terbentuk, yaitu 7,68 mm pada 55%, 9,85 mm pada 75%, dan 10,86 mm pada 95%. Nilai ANOVA menunjukkan adanya perbedaan yang signifikan antarperlakuan (p < 0,001). Aktivitas antibakteri ekstrak daun kelor tergolong sedang hingga kuat dan bersifat bakteriostatik, yaitu menghambat pertumbuhan tanpa membunuh bakteri secara total. Dengan demikian, ekstrak etanol daun kelor berpotensi dikembangkan sebagai agen antibakteri alami alternatif terhadap Escherichia coli. Kata kunci: antibakteri, ekstrak etanol, Escherichia coli., zona hambat, Moringa oleifera L.,
Co-Authors Adinda Laura Munthe Adinda Rizky Pratiwi Agustina Matontang Ahmad Shafwan Pulungan Alyvia Salsabila Amelia Sasmita Br Sianturi Astria Ayu Ramadianti Bunga Ria Sitanggang Deka Wira Pratama Barus Dewi Novina Sukapiring, Dewi Novina Emy Hariati Fanny Hafifa Fatimah Pohan Gultom, Endang Sulistyarini Habil Akbar Hendri Irawan Juhardi Sembiring Leni Malinda Lydia Siburian Mastari Ramadhani Muhammad Khaibar Putra Adhitia Najwa Sarip Nita Maya Sari Pane Nur Afliza Nuri Ramadhan Nurul Ajimah Ritonga, Merdayana, Sakdan Azwan Nurwijayanti Perry Boy Chandra Siahaan Rara Astili Siregar Rika Lidia Sibarani Rizka Juliana Siregar Rizky Ananda Sari, Vio Joana Sisilia Florina Yanti Suharsih Suharsih Syarifah Ainun Harahap Widya Purnama Sitanggang Widyanti Saragi Zihan Zahriani Batubara