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All Journal Jurnal Sain Veteriner Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Indonesian Journal of Cancer Chemoprevention Journal of Mathematical and Fundamental Sciences Pharmacon INDONESIAN JOURNAL OF PHARMACY Universa Medicina Jurnal Ilmu Kefarmasian Indonesia Jurnal Layanan Masyarakat (Journal of Public Service) Jurnal Farmasi Sains dan Praktis
Iwan Sahrial Hamid
Faculty Of Pharmacy, Universitas Airlangga Surabaya
Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
CYP1A1 and GST expression of hepatocytes induced by 7,12-dimethylbenz(a)anthracene and the influence of ethanolic extract of Gynura procumbens Iwan Sahrial Hamid
Indonesian Journal of Pharmacy Vol 20 No 4, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.093 KB) | DOI: 10.14499/indonesianjpharm0iss0pp198-206

Abstract

The cytochrome P-450 (CYP) and glutathione S-transferase (GST) enzyme systems may influence the biological effects of carcinogens. As such, these enzymes may predict the developmental risk of breast cancer, as well as be potential targets for chemoprevention. The purpose of this study was to compare the expression of CYP1A1 and GSTµ between treatment groups. Expression of CYP1A1 and GSTµ was quantified in liver tissue from 18 female Sprague Dawley rats aged 40 days, which randomly divided into six treatment groups. Those are base line group (without DMBA and ethanolic extract treatment), DMBA induced cancer group, the other two groups was administrated by DMBA after treated by ethanolic extract in two different doses, 300 mg /kg BW and 750 mg/kg BW.  The last two groups were given two doses of extract ethanolic only, without initiated of DMBA. The ingestion of the extract was carried for three weeks and the ingestion of DMBA was performed twice in the third week. The expression CYP1A1 and GST was quantified by immunohistochemistry. CYP1A1 expression was significantly higher (P < 0.05) in cancer group (DMBA) as compared with other groups. On the other hand, GST expression was lower in cancer group (P < 0.05). Result of this study demonstrated that ethanolic extract leaves of  G. procumbens in 300 mg/kg BW dose could  inhibit CYP1A1 stronger than are other and induced GSTµ level. Has effect on ethanolic extract leaves of G. procumbens has ability in played role of as blocking agent in preventing initiation stage of carsinogenesis, therefore it should be taken into account for chemopreventing agent in mammary carsinogenesis.Key words : Gynura procumbens, breast cancer,  hemopreventive, CYP1A1, GSTμ
CYP1A1 and GSTm expression of hepatocytes induced by 7,12-dimethylbenz(a)anthracene and the influence of ethanolic extract of Gynura procumbens Iwan Sahrial Hamid; Sugiyanto .; Edy Meiyanto; Sitarina Widyarini
Indonesian Journal of Pharmacy Vol 20 No 4, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.093 KB) | DOI: 10.14499/indonesianjpharm0iss0pp198-206

Abstract

The cytochrome P-450 (CYP) and glutathione S-transferase (GST) enzyme systems may influence the biological effects of carcinogens. As such, these enzymes may predict the developmental risk of breast cancer, as well as be potential targets for chemoprevention. The purpose of this study was to compare the expression of CYP1A1 and GST* between treatment groups. Expression of CYP1A1 and GST* was quantified in liver tissue from 18 female Sprague Dawley rats aged 40 days, which randomly divided into six treatment groups. Those are base line group (without DMBA and ethanolic extract treatment), DMBA induced cancer group, the other two groups was administrated by DMBA after treated by ethanolic extract in two different doses, 300 mg /kg BW and 750 mg/kg BW. The last two groups were given two doses of extract ethanolic only, without initiated of DMBA. The ingestion of the extract was carried for three weeks and the ingestion of DMBA was performed twice in the third week. The expression CYP1A1 and GST was quantified by immunohistochemistry. CYP1A1 expression was significantly higher (P < 0.05) in cancer group (DMBA) as compared with other groups. On the other hand, GST expression was lower in cancer group (P < 0.05). Result of this study demonstrated that ethanolic extract leaves of G. procumbens in 300 mg/kg BW dose could inhibit CYP1A1 stronger than are other and induced GST* level. Has effect on ethanolic extract leaves of G. procumbens has ability in played role of as blocking agent in preventing initiation stage of carsinogenesis, therefore it should be taken into account for chemopreventing agent in mammary carsinogenesis.Key words : Gynura procumbens, breast cancer, Chemopreventive, CYP1A1, GSTμ
Co-Authors Alif Ekacahya Wahyudi Angelica Kresnamurti Bimo Aksono Chintya Wandasari Diyah, Nuzul Wahyuning Dwi Damayanti Rasdianto Edy Meiyanto Faisal Fikri Febriana Siswulandari Hardiyono Hardiyono Jennifer Rachel Willyanto Juni Ekowati Kholis Amalia Nofianti Lailatul Muniroh Lucia Hendriati Lucia Hendriati Muhammad Thohawi Elziyad Purnama Ngakan Made Rai Widjaja Putu Mirah Risata Regiskha Hermandanie Surya Retno Bijanti Rondius Solfaine Saputro, Amung Logam Siswandono, Siswandono Sitarina Widyarini Sugiyanto . Suko Hardjono Teguh Widodo Teguh Widodo Teguh Widodo Vivi Kariyaningtias Yuseni Kusuma P.