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Journal : Cassowary

Analisis Molekuler Bakteri Endofit SH 14 dari Mangrove Scyphiphora hydrophyllacea Pantai Rendani Yamco, Fallen; Massora, Maria; A. Mogea, Rina
Cassowary Vol 7 No 2 (2024): Juni
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v7.i2.284

Abstract

ABSTRACT: The mangrove endophytic bacterial isolate Scyphiphora hydrophyllacea has antimicrobial capabilities and also has other bioactive compounds to be developed in the field of biotechnology. The aim of this study was to identify the endophytic bacterial isolate SH 14 obtained from the S. hydrophyllacea mangrove based on 16S rDNA sequence analysis. Molecular-based identification of bacteria, namely DNA amplified by Polymerase Chain Reaction (PCR) using primers 27F and 1492R and has a gene amplification of 1481 bp. Sequence data analyzed using the BLAST program showed that isolate SH 14 had a homology level of 99.43% with Brevibacillus brevis. This similarity value is a reference in determining bacterial identification, where bacteria are categorized as the same species if they have a similarity of 99%.
Identifikasi molekuler bakteri indigenous penghasil enzim amilase dari pati sagu asal Poom Kepulauan Yapen Hesron; Massora, Maria; A. Mogea, Rina; Wanggai. I. M; Utami. P
Cassowary Vol 8 No 3 (2025): Juli
Publisher : Program Pascasarjana Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/casssowary.cs.v8.i3.320

Abstract

ABSTRACT: This study aimed to identify indigenous bacterial isolates producing Amylase ezyme from sago starch from Poom, Yapen Islands, using the 16S rDNA sequence molecular method. The isolates identified were IA4 and IA8, which exhibited the highest values of assimilase enzyme activity. Bacteria were identified based on DNA amplification using Polymerase Chain Reaction (PCR) with primers 27F and 1492R. Based on the results of molecular identification, the similarity index of Isolate IA4 and Bacillus cereus D21 is 97.80%, while isolate IA8 had a similarity index of 99.79% with Alcaligenes faecalis Y5. Therefore, the amylolytic bacterial isolates were named Bacillus cereus strain IA4 and Alcaligenes faecalis strain IA8.