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Aktivitas Antibakteri Ekstrak Kulit Batang Belimbing Wuluh Terhadap Escherichia coli Penyebab Diare Jade Septhimoranie; Dwi Aditiyarini; Vinsa Cantya Prakasita
Bioeksperimen: Jurnal Penelitian Biologi Vol 8, No 2: September 2022
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v8i2.15749

Abstract

Diare merupakan salah satu gejala infeksi pada saluran cerna, yang dapat disebabkan oleh adanya organisme (bakteri, virus, dan/atau parasit), sehingga menyebabkan feses menjadi lebih cair dan frekuensinya lebih sering dari biasanya. Escherichia coli memiliki presentase yang tinggi dibandingkan bakteri lain pada feses penderita diare. Pengobatan yang dilakukan biasanya dengan menggunakan antibiotik, namun sekarang ini banyak ditemukan antibiotik yang telah resisten. Tanaman obat-obatan dapat digunakan sebagai alternatif dalam mengatasi diare. Salah satunya ialah kulit batang Belimbing Wuluh. Adanya kandungan senyawa yang bersifat antibakteri pada kulit batang Belimbing Wuluh dimungkinkan dapat menghambat pertumbuhan E. coli penyebab diare. Tujuan penelitian adalah untuk mengetahui aktivitas antibakteri ekstrak kulit batang Belimbing Wuluh terhadap E. coli penyebab diare. Pada penelitian ini menggunakan metode ekstraksi dekoktasi. Uji fitokimia dilakukan terhadap flavonoid, alkaloid, dan saponin. Uji total fenol dilakukan dengan menggunakan metode Folin-Ciocalteu. Uji antibakteri dilakukan dengan menggunakan disk diffusion method Kirby-Bauer, dilanjutkan uji MIC dengan metode mikrodilusi serta uji MBC. Analisis data dengan One-Way ANOVA dan post-hoc Duncan. Kulit batang Belimbing Wuluh mengandung senyawa saponin, alkaloid, flavonoid, dan fenol. Hasil rata-rata dari nilai total fenol adalah 25,12 ± 7,73(mg GAE)⁄g. Hasil uji aktivitas antibakteri menunjukkan bahwa ekstrak kulit batang Belimbing Wuluh dengan konsentrasi 2,5% masih dapat menghambat E. coli secara signifikan (P≤0,05).
Profiling secondary metabolites and antioxidant activity of tea mistletoe leaves (Scurrula artopurpurea (Bl.) Danser) in Nglinggo, Kulon Progo, Yogyakarta Dwi Aditiyarini; Ratih Restiani; Evieyana Evieyana
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 2 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i2.31258

Abstract

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Antioxidant and Antibacterial Activity of Pomegranate Extract (Punica granatum L.) in Lip Balm Formulation Maria Grasela Kase; Aniek Prasetyaningsih; Dwi Aditiyarini
Biology, Medicine, & Natural Product Chemistry Vol 12, No 1 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.121.109-117

Abstract

Lips are a part of the face that does not have hair follicles making them easily dry and crack. Lipbalm is a beauty product that could be used to solve this problem by increasing lip moisture. However, the synthetic ingredient in commercial lip balm can have side effects on the body in the long term. Pomegranate fruit is one of the natural ingredients containing anthocyanin which can be used as a natural dye, antioxidant, and antibacterial. Therefore, this study was performed to study the potency of pomegranates as natural dyes, antioxidants, and antibacterial in lip balm. Pure pomegranate juice was obtained through squeeze step. Lip balm was prepared in several concentrations of pomegranate juice which were 0%, 12.5%, 18.75%, and 25%. Phytochemical screening shows the content of anthocyanin, saponin, tannin, and flavonoid. In this study, pomegranate juice has low antioxidant activity with IC50 449 ppm. Lip balm formula with 18.75% and 25% of pomegranate juice can inhibit the growth of the bacteria Staphylococcus aureus.
Potensi Minyak Sereh Wangi (Cymbopogon nardus) sebagai Antibakteri terhadap Propionibacterium acnes dan Staphylococcus aureus pada Sediaan Gel Antijerawat Kania Apenta Olisvelos; Dwi Aditiyarini; Aniek Prasetyaningsih
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Acne can be caused by the bacteria Propionibacterium acnes and Staphylococcus aureus. One way to treat acne is to use antibiotics. However, this can create resistance. In this study, citronella oil will be formulated into an anti-acne gel preparation with various concentrations of 10%, 15% and 20%. The study began with distillation of citronella oil and analysis and identification of compounds. Then the gel was made by mixing citronella oil in the HPMC gel base and then the gel preparation was tested for antibacterial activity by the disc diffusion method and physical preparation tests included organoleptic tests, pH measurements, viscosity tests, homogeneity tests, dispersion tests, gel stability tests. Based on the results of compound analysis using GC-MS, citronella oil contains geraniol (55.05%). Of the three formulations, the preparation that has good gel physical properties based on its physical properties is F2 with 15% citronella oil content. In the antibacterial test, the results showed that citronella oil gel had the ability to inhibit Propionibacterium acnes and Staphylococcus aureus with the most optimal formulation being F2 with an average inhibition of 1.5 cm.
Aktivitas Antioksidan dan Antibakteri Ekstrak Etanol Adas Pagar (Eupatorium Capillifolium (Lam.) Small) terhadap Staphylococcus Aureus dan Escherichia Coli Feblicya Palimbunga; Dwi Aditiyarini; Vinsa Cantya Prakasita
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 6 No 1 (2023): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v6i1.5361

Abstract

The purpose of the study was to investigate the presence of second-order metabolites and the antibacterial and antifungal activities against S. aureus and E. coli in an experimental manner. This study is being conducted using an experimental method. Metabolit is quickly identified by scrining fitokimia. Antioxidant activity was measured using the DPPH method. Antibacterial activity is carried out using the cakram diffusion method. Ekstraksi was carried out by the 96% etanol-maseration process. The results show that the extracted etanol adas pagar contains flavonoid, alkaloid, tanin, saponin, and terpenoid content. Ekstrak etanol adas pagar has strong anti-oxidant activity with an IC50 of 80,56 ppm. Due to its antibacterial potential, ekstrak etanol adas pagar can kill gram-positive Staphylococcus aureus as well as gram-negative Escherichia coli bacteria when applied in concentrations of 100% and 50%, respectively. The main finding of this study is that ekstrak etanol adas pagar can be used as a safe antibacterial and antifungal against the growth of S. aureus and E. coli. Keywords: Adas Pagar, Antibacterial, Antioxidant, Extraction, Metabolite
The Effect of Citric Acid on Antioxidant and Antibacterial Activities of Butterfly Flower Extract (Clitoria ternatea L.) Dwi Aditiyarini; Aniek Prasetyaningsih; Marsauli Manalu
Metamorfosa: Journal of Biological Sciences Vol 10 No 2 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i02.p05

Abstract

Telang (Clitoria ternatea L.) merupakan suatu tanaman perdu dengan bunga berwarna biru yang telah dimanfaatkan oleh masyarakat sebagai pewarna alami pada minuman karena dipercaya memiliki aktivitas antioksidan. Saat dikonsumsi, minuman ini ditambahkan perasan air jeruk untuk memberikan rasa. Penelitian ini bertujuan untuk menganalisis pengaruh asam sitrat terhadap aktivitas antioksidan dan antibakteri bunga telang. Penelitian ini diawali dengan ekstraksi bunga telang pada tiga perlakuan asam yaitu tanpa penambahan asam, pH 2 dan pH 4 dengan penambahan asam sitrat. Skrining fitokimia, uji daya hambat dan aktivitas antioksidan dilakukan terhadap tiga ekstrak. Skrining fitokimia menunjukkan adanya kandungan tanin, saponin, terpenoid, flavonoid, alkaloid dan antosianin. Hasil uji daya hambat menunjukkan aktivitas antibakteri tertinggi pada ekstrak pH 2 dengan diameter zona hambat sebesar 39 ± 0,153 mm pada Staphylococcus aureus dan 28 ± 0,10 mm pada Escherichia coli, yang tergolong sangat kuat. Selain itu, ekstrak pH 2 juga memiliki aktivitas antioksidan paling tinggi dibandingkan dua perlakuan lainnya dengan nilai IC50 136 ppm yang dikategorikan aktivitas antioksidan sedang. Data tersebut menunjukkan bahwa ekstraksi bunga telang dengan asam sitrat pada kondisi pH 2 mampu meningkatkan aktivitas antibakteri dan antioksidan ekstrak bunga telang yang dapat digunakan dalam pengembangan makanan fungsional. Kata kunci: antibakteri, antioksidan, ekstrak bunga telang, pH.
Effect of Plant Growth Regulators (PGRs) on Biomass and Flavonoid Production of Talinum paniculatum Callus Culture Sarah Mega Pratenna Kaban; Ratih Restiani; Dwi Aditiyarini
Jurnal Biodjati Vol 9, No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.33788

Abstract

Talinum paniculatum Jacq (Gaertn), locally known as Javanese Ginseng, is an essential medicinal plant characterized by high flavonoid content with antioxidant and antimicrobial activities. Compared to other natural sources, a larger number of T. paniculatum leaves is required to obtain high amounts of flavonoid for drug development. In this context, in vitro culture can be used to increase flavonoid production in a controlled condition without reducing plant population. The concentration of Plant Growth Regulators (PGRs) is capable of influencing biomass and flavonoid accumulation in callus culture from several species. Despite these benefits, there is limited information about the optimal concentration of PGRs for flavonoid production from T. paniculatum callus culture. Therefore, this study aimed to investigate the optimum concentration of 2,4-D and kinetin for callus growth, as well as flavonoid production from T. paniculatum. The investigation was carried out using a completely randomized design (CRD), where young leaves explants were cultured on MS media supplemented with various concentrations of 2,4-D (0, 0.5, 1, 2) mg/L and kinetin (0, 0.5, 1, 2) mg/L for callus and flavonoid production. The results showed that the highest callus biomass of 0.105 g was produced from MS medium with 2 mg/L of 2,4-D and 1 mg/L of kinetin. Meanwhile, the highest total flavonoid content of 25.66 mg QE/g DW was produced from media supplemented with 1 mg/Lof 2,4-D and 2 mg/L of kinetin. These results showed that different combinations of 2,4-D and kinetin were required to increase biomass and flavonoid production. In conclusion, this study provided valuable information for the development of bioactive compounds through in vitro culture.
Antibacterial Activity of Scurrula ferruginea (Roxb. Ex Jack) Leaves Extract Against Staphylococcus aureus and Escherichia coli Dwi Aditiyarini; Nita Sonia Sipayung; Vinsa Cantya Prakasita
Jurnal Biodjati Vol 9, No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30445

Abstract

The mistletoe (Scurrula ferruginea) is a parasitic plant obtaining nutrients and water from host plants at Nglinggo tea plantation, Kulon Progo, Yogyakarta. Despite the numerous benefits of Scurrula ferruginea leaf, substantiation regarding its pharmacological activity is still lacking. Therefore, this research aimed to identify Scurrula ferruginea species and obtain secondary metabolites with antibacterial activity. The experiment was carried out by extracting Scurrula ferruginea leaf using maceration methods with 96% ethanol, followed by qualitatively and quantitatively phytochemical screening, inhibitory zone test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. Several compounds, such as flavonoids, tannins, phenols, saponins, alkaloids, terpenoids, and steroids, were identified in the mistletoe extract. The results showed total flavonoid, phenolic, and tannin levels were 7.41 ± 0.38 mg QE/mg, 1672 ± 50.99 mg GAE/g, and 1.43 ± 0.15 mg TAE/g, respectively. Scurrula ferruginea leaf ethanolic extract showed the potential to form an inhibition zone against S. aureus in the moderate category at 525 mg/mL, but no significant activity was observed against E. coli. The MIC results of Scurrula ferruginea leaf ethanolic extract against S. aureus were 0.6%, and the MBC was at 2.4%. Furthermore, the MIC value against E. coli was 14%, and the MBC value was 28%. Based on these results, it could be concluded that Scurrula ferruginea leaf ethanolic extract showed high effectiveness in inhibiting and killing S. aureus bacteria compared to E. coli, with MIC and MBC values of 0.6% and 2.4%, respectively
Antidiabetic Potential of Ethanol Extract from Moringa oleifera Leaves in Streptozotocin-Nicotinamide-Induced Female Mus musculus Vinsa Cantya Prakasita; Nadya Aprina Theodora; Enjelin Anjung Susilowati; Dwi Aditiyarini; Aniek Prasetyaningsih
Jurnal Biodjati Vol 9, No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30699

Abstract

Type 2 diabetes mellitus (DM) is a chronic metabolic dis­order characterized by elevated blood glucose levels due to insulin resistance. Type 2 diabetes is considerably more prevalent than oth­er forms (85–90%). The risk of type 2 diabetes is higher in women (53.2%). There is an urgent requirement for better and more afforda­ble treatment options considering DM therapy is expensive and may have adverse health effects. The study's objective is to examine how acute toxicity, blood glucose levels, and body weight are affected by Moringa leaf ethanol extract (MLEE). Maceration was employed to eliminate the leaves of the moringa plant. Phytochemical screening was completed to assess the total flavonoid content and screen for alkaloids, flavonoids, phenolics, saponins, and tannins. Acute toxicity testing was performed following OECD guideline 423. Clinical symp­toms of acute toxicity were observed every 30 minutes for the first 24 hours post-treatment, followed by observations every 24 hours up to 14 days. The estimated LD50 range was determined. Streptozotocin/ nicotinamide-induced female Mus musculus was administered to eval­uate the antidiabetic potential of MLEE. Six groups of mice were uti­lized, which included a healthy control group (aquades not induced), a negative control group (induced aquades), a positive control group (induced glimepiride 0.8 mg/kg BW), and three treatment groups with varying dosages of MLEE (induced; 0, 100, and 150 mg/kg BW). A semi-auto chemical analyzer was employed on days 0 through 31 to determine blood glucose levels. An analytical digital balance was utilized to calculate the body weight. With a total flavonoid concen­tration of 20.75%, MLEE incorporated alkaloids, flavonoids, pheno­lics, saponins, and tannins. MLEE demonstrated a significant effect in lowering blood glucose levels at a dose of 100 mg/kg BW (P<0.05). A significant positive correlation has been identified between body weight and blood glucose levels (P<0.05).
KOMPOSISI NUTRIEN SAMPAH ORGANIK SISA NASI DAN TULANG IKAN NILA DAN PENGARUHNYA BAGI PERTUMBUHAN LARVA BLACK SOLDIER FLY (Hermetia illucens L.) Abrillian, Albert; Aditiyarini, Dwi; Madyaningrana, Kukuh
Jurnal Pro-Life Vol. 10 No. 3 (2023): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Organic waste as a by-product of household activities that are produced continuously is still a major problem in Indonesia. Conventional organic waste management still has weaknesses and limitations. Black soldier fly larvae are decomposers that can be used in organic waste management. This study aims to measure the nutrient levels of organic waste used, such as leftover rice, tilapia bones, or a combination of both and study the use of organic waste for the growth of BSF larvae. The research used 150 BSF larvae aged 12 days with three repeats and four test treatments: T51 feed (control), leftover rice, tilapia bones, and a combination of leftover rice and tilapia bones (50:50). The highest levels of carbohydrates and fats were detected in the remaining rice and tilapia bones, respectively. The type of feed has an impact on the nutrients and growth of BSF larvae. The combination treatment produces larvae with the largest biomass and shortest growth time, on the other hand, a single treatment extends the growth time of larvae with a relatively small biomass. The combination treatment between leftover rice and tilapia bones (50:50) is the best type of feed in supporting the growth of BSF larvae, while single feed treatment is considered inappropriate for the growth of BSF larvae.